Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0651
(837 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g15230.1 68415.m01737 lipase family protein similar to SP|P07... 90 1e-18
At5g14180.1 68418.m01658 lipase family protein similar to SP|Q64... 79 3e-15
At1g73920.2 68414.m08561 lipase family protein similar to lipase... 64 1e-10
At1g73920.1 68414.m08560 lipase family protein similar to lipase... 64 1e-10
At1g18460.1 68414.m02303 lipase family protein similar to triacy... 62 6e-10
At1g53080.1 68414.m06010 legume lectin family protein 33 0.31
At5g03350.1 68418.m00288 legume lectin family protein contains P... 32 0.54
At1g15060.1 68414.m01800 expressed protein 31 0.72
At4g25090.1 68417.m03604 respiratory burst oxidase, putative / N... 31 1.3
At4g36610.1 68417.m05196 hydrolase, alpha/beta fold family prote... 30 1.7
At2g26720.1 68415.m03205 plastocyanin-like domain-containing pro... 30 1.7
At5g40210.1 68418.m04879 nodulin MtN21 family protein similar to... 29 2.9
At1g61060.1 68414.m06874 F-box family protein contains Pfam prof... 28 6.7
At3g57570.1 68416.m06410 expressed protein 28 8.8
At1g65630.1 68414.m07444 DegP protease, putative contains simila... 28 8.8
>At2g15230.1 68415.m01737 lipase family protein similar to SP|P07098
Triacylglycerol lipase, gastric precursor (EC 3.1.1.3)
{Homo sapiens}; contains Pfam profile PF04083:
ab-hydrolase associated lipase region
Length = 393
Score = 90.2 bits (214), Expect = 1e-18
Identities = 42/102 (41%), Positives = 59/102 (57%)
Frame = +1
Query: 274 PAVLVMHGLFASAADWVLMGPGVGLAYILAEAGYDVWLGNARGTYYSRAHIKLDPDNDSE 453
P VL+ HGLF + W L P L +ILA+ G+DVW+GN RGT YS H+ L D D E
Sbjct: 73 PPVLLQHGLFMAGDVWFLNSPKESLGFILADHGFDVWVGNVRGTRYSYGHVTLS-DTDKE 131
Query: 454 FWKFSWEEIGTRDLPAMIVTL*KLLEKDDFIILVTHKELLSF 579
FW +SW+++ DL MI L + F++ + ++SF
Sbjct: 132 FWDWSWQDLAMYDLAEMIQYLYSISNSKIFLVGHSQGTIMSF 173
Score = 31.1 bits (67), Expect = 0.95
Identities = 19/78 (24%), Positives = 41/78 (52%), Gaps = 1/78 (1%)
Frame = +3
Query: 537 RLHYIGHSQGTTVFWAMGSLRPEYNSKIIAMQAYAPVAYLE-FNANRLLKLIAPHANSIE 713
++ +GHSQGT + +A +P + A P++YL+ A + +++ H ++
Sbjct: 159 KIFLVGHSQGTIMSFA-ALTQPHVAEMVEAAALLCPISYLDHVTAPLVERMVFMH---LD 214
Query: 714 ALTSLIGINELFGRSDSL 767
+ +G++++ RSD L
Sbjct: 215 QMVVALGLHQINFRSDML 232
Score = 29.1 bits (62), Expect = 3.8
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = +2
Query: 152 LIKKYRYPVEVHKVVTEDGYILEMQRI 232
LI Y H + T+DGYIL +QR+
Sbjct: 36 LIHPANYSCTEHSIQTKDGYILALQRV 62
>At5g14180.1 68418.m01658 lipase family protein similar to SP|Q64194
Lysosomal acid lipase/cholesteryl ester hydrolase
precursor (EC 3.1.1.13) {Rattus norvegicus}; contains
Pfam profile PF04083: ab-hydrolase associated lipase
region
Length = 418
Score = 79.4 bits (187), Expect = 3e-15
Identities = 34/79 (43%), Positives = 49/79 (62%)
Frame = +1
Query: 268 KKPAVLVMHGLFASAADWVLMGPGVGLAYILAEAGYDVWLGNARGTYYSRAHIKLDPDND 447
K+ VL+ HG+ W+L L ILA+ G+DVW+GN RGT +SR H L+P +
Sbjct: 95 KRQPVLIQHGILVDGMSWLLNPADQNLPLILADQGFDVWMGNTRGTRFSRRHKYLNP-SQ 153
Query: 448 SEFWKFSWEEIGTRDLPAM 504
FW ++W+E+ + DLPAM
Sbjct: 154 RAFWNWTWDELVSYDLPAM 172
Score = 42.3 bits (95), Expect = 4e-04
Identities = 18/27 (66%), Positives = 20/27 (74%)
Frame = +2
Query: 164 YRYPVEVHKVVTEDGYILEMQRIPHGR 244
+ Y E H VVT+DGYIL MQRIP GR
Sbjct: 59 FGYKCEEHDVVTQDGYILNMQRIPEGR 85
Score = 29.9 bits (64), Expect = 2.2
Identities = 23/75 (30%), Positives = 41/75 (54%)
Frame = +3
Query: 534 RRLHYIGHSQGTTVFWAMGSLRPEYNSKIIAMQAYAPVAYLEFNANRLLKLIAPHANSIE 713
+++HY+GHS GT + +A S + + ++ + +PVAYL + ++ IA E
Sbjct: 182 QKIHYLGHSLGTLIGFASFSEKGLVD-QVRSAAMLSPVAYLS-HMTTVIGDIAAKTFLAE 239
Query: 714 ALTSLIGINELFGRS 758
A TS++G E +S
Sbjct: 240 A-TSILGWPEFNPKS 253
>At1g73920.2 68414.m08561 lipase family protein similar to lipase
GB:CAA74737 [SP|O46108] from [Drosophila melanogaster]
Length = 635
Score = 63.7 bits (148), Expect = 1e-10
Identities = 36/84 (42%), Positives = 49/84 (58%)
Frame = +1
Query: 256 RPDPKKPAVLVMHGLFASAADWVLMGPGVGLAYILAEAGYDVWLGNARGTYYSRAHIKLD 435
R D +K AV + HG+ S+ WV G A+ + GYDV+LGN RG SR H+ +
Sbjct: 258 RRDARK-AVFLQHGVLDSSMGWVSNGVVGSPAFAAYDQGYDVFLGNFRG-LVSRDHVNKN 315
Query: 436 PDNDSEFWKFSWEEIGTRDLPAMI 507
+ EFW++S E GT D+PAMI
Sbjct: 316 -ISSKEFWRYSINEHGTEDIPAMI 338
Score = 34.3 bits (75), Expect = 0.10
Identities = 13/28 (46%), Positives = 20/28 (71%)
Frame = +2
Query: 152 LIKKYRYPVEVHKVVTEDGYILEMQRIP 235
+I + YP E +V+T DGY+L ++RIP
Sbjct: 230 VITELGYPYEAIRVITSDGYVLVLERIP 257
>At1g73920.1 68414.m08560 lipase family protein similar to lipase
GB:CAA74737 [SP|O46108] from [Drosophila melanogaster]
Length = 704
Score = 63.7 bits (148), Expect = 1e-10
Identities = 36/84 (42%), Positives = 49/84 (58%)
Frame = +1
Query: 256 RPDPKKPAVLVMHGLFASAADWVLMGPGVGLAYILAEAGYDVWLGNARGTYYSRAHIKLD 435
R D +K AV + HG+ S+ WV G A+ + GYDV+LGN RG SR H+ +
Sbjct: 327 RRDARK-AVFLQHGVLDSSMGWVSNGVVGSPAFAAYDQGYDVFLGNFRG-LVSRDHVNKN 384
Query: 436 PDNDSEFWKFSWEEIGTRDLPAMI 507
+ EFW++S E GT D+PAMI
Sbjct: 385 -ISSKEFWRYSINEHGTEDIPAMI 407
Score = 34.3 bits (75), Expect = 0.10
Identities = 13/28 (46%), Positives = 20/28 (71%)
Frame = +2
Query: 152 LIKKYRYPVEVHKVVTEDGYILEMQRIP 235
+I + YP E +V+T DGY+L ++RIP
Sbjct: 299 VITELGYPYEAIRVITSDGYVLVLERIP 326
>At1g18460.1 68414.m02303 lipase family protein similar to
triacylglycerol lipase, gastric precursor (EC 3.1.1.3)
{Canis familiaris} [SP|P80035]
Length = 701
Score = 61.7 bits (143), Expect = 6e-10
Identities = 35/84 (41%), Positives = 49/84 (58%)
Frame = +1
Query: 256 RPDPKKPAVLVMHGLFASAADWVLMGPGVGLAYILAEAGYDVWLGNARGTYYSRAHIKLD 435
R D +K AV + HG+ S+ WV G A+ + GYDV+LGN RG SR H+K +
Sbjct: 325 RRDARK-AVYLQHGVMDSSMGWVSNGVVGSPAFAAYDQGYDVFLGNFRG-LVSRDHVKKN 382
Query: 436 PDNDSEFWKFSWEEIGTRDLPAMI 507
+ +FW++S E T D+PAMI
Sbjct: 383 -ISSKDFWRYSINEHATEDIPAMI 405
Score = 31.5 bits (68), Expect = 0.72
Identities = 14/28 (50%), Positives = 19/28 (67%)
Frame = +2
Query: 152 LIKKYRYPVEVHKVVTEDGYILEMQRIP 235
+I + YP E +VVT DGY L ++RIP
Sbjct: 297 VITELGYPYEAIRVVTSDGYGLLLERIP 324
>At1g53080.1 68414.m06010 legume lectin family protein
Length = 283
Score = 32.7 bits (71), Expect = 0.31
Identities = 13/45 (28%), Positives = 24/45 (53%)
Frame = +1
Query: 148 WTYQKI*ISSRSSQGRYGRWLYLRNAKNSSWTRPEHRPDPKKPAV 282
W ++++ +SS + Y W+ +N+K W P H PK+P +
Sbjct: 199 WLFKEVKLSSGDN---YKAWIEYKNSKVIVWLAPAHLKKPKRPLI 240
>At5g03350.1 68418.m00288 legume lectin family protein contains Pfam
domain, PF00139: Legume lectins beta domain
Length = 274
Score = 31.9 bits (69), Expect = 0.54
Identities = 24/79 (30%), Positives = 37/79 (46%)
Frame = +1
Query: 148 WTYQKI*ISSRSSQGRYGRWLYLRNAKNSSWTRPEHRPDPKKPAVLVMHGLFASAADWVL 327
W++++ +S+ RY W+ RN+K + PE PKKP ++V H L S
Sbjct: 190 WSFKEFKLSNGE---RYKAWIEYRNSKVTVTLAPETVKKPKKP-LIVAH-LDLSKVFLQN 244
Query: 328 MGPGVGLAYILAEAGYDVW 384
M PG A +D+W
Sbjct: 245 MYPGFSGAMGRGVERHDIW 263
>At1g15060.1 68414.m01800 expressed protein
Length = 578
Score = 31.5 bits (68), Expect = 0.72
Identities = 23/64 (35%), Positives = 37/64 (57%), Gaps = 2/64 (3%)
Frame = +3
Query: 537 RLHYIGHSQGTTVFWAMGSLRPEYNSKIIAMQAYAPVAY-LEFN-ANRLLKLIAPHANSI 710
+L IGHS G + +AM S R + + ++ A A +A +++ +N LKL+ P AN
Sbjct: 356 KLFAIGHSMGGILLYAMLS-RCAFEGREPSVAAVATLASSVDYTTSNSALKLLIPLANPA 414
Query: 711 EALT 722
EAL+
Sbjct: 415 EALS 418
>At4g25090.1 68417.m03604 respiratory burst oxidase, putative /
NADPH oxidase, putative similar to respiratory burst
oxidase protein A from Arabidopsis thaliana, gb:AF055353
[gi:3242781], protein D [gi:3242789]; contains Pfam
profile PF01794 Ferric reductase like transmembrane
component
Length = 849
Score = 30.7 bits (66), Expect = 1.3
Identities = 13/41 (31%), Positives = 25/41 (60%)
Frame = +1
Query: 415 RAHIKLDPDNDSEFWKFSWEEIGTRDLPAMIVTL*KLLEKD 537
++H+K D ++E KF WE+I + + ++T L++KD
Sbjct: 152 KSHLKGDVITETELKKF-WEQINDKSFDSRLITFFDLMDKD 191
>At4g36610.1 68417.m05196 hydrolase, alpha/beta fold family protein
low similarity to
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase
[Pseudomonas fluorescens] GI:1871461; contains Pfam
profile PF00561: hydrolase, alpha/beta fold family
Length = 317
Score = 30.3 bits (65), Expect = 1.7
Identities = 14/32 (43%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
Frame = +1
Query: 205 WLYLRNAKNSSWTRPEHRPD-PKKPAVLVMHG 297
W+ K +S T +PD PKKP VL++HG
Sbjct: 37 WVPKETLKKNSGTGKPTKPDKPKKPVVLLIHG 68
>At2g26720.1 68415.m03205 plastocyanin-like domain-containing
protein / mavicyanin, putative similar to mavicyanin
SP:P80728 from [Cucurbita pepo]
Length = 206
Score = 30.3 bits (65), Expect = 1.7
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = -2
Query: 371 PASAKMYAKPTPGPIKTQSAAEANSP 294
PAS A P PGP+++QS++ + SP
Sbjct: 127 PASLGHVAVPVPGPVRSQSSSSSPSP 152
>At5g40210.1 68418.m04879 nodulin MtN21 family protein similar to
MtN21 GI:2598575 (root nodule development) from
[Medicago truncatula]
Length = 339
Score = 29.5 bits (63), Expect = 2.9
Identities = 18/53 (33%), Positives = 27/53 (50%), Gaps = 2/53 (3%)
Frame = +1
Query: 280 VLVMHG--LFASAADWVLMGPGVGLAYILAEAGYDVWLGNARGTYYSRAHIKL 432
V + HG L +S +DW++ G + L YIL Y V + + G Y S + L
Sbjct: 153 VTLYHGPMLMSSHSDWIIGGGLLALQYILVSVSYLV-MAHTMGRYPSAVVVTL 204
>At1g61060.1 68414.m06874 F-box family protein contains Pfam profile
PF00646: F-box domain
Length = 267
Score = 28.3 bits (60), Expect = 6.7
Identities = 19/77 (24%), Positives = 31/77 (40%), Gaps = 1/77 (1%)
Frame = +3
Query: 504 DCYTLKVAGKRRLHYIGHSQGTTVFWAMGSLRPEYNSKIIAMQAYAP-VAYLEFNANRLL 680
D +T K+ K + I + + W+ RP YN M P + + NA L
Sbjct: 23 DLFTAKILSKLPVKSIAQCRCVSKLWSSQIRRPYYNMLFPIMSPAPPRILFTIENAEGLF 82
Query: 681 KLIAPHANSIEALTSLI 731
+P + + TSL+
Sbjct: 83 FYTSPQPRNPDENTSLV 99
>At3g57570.1 68416.m06410 expressed protein
Length = 1057
Score = 27.9 bits (59), Expect = 8.8
Identities = 14/30 (46%), Positives = 20/30 (66%)
Frame = +3
Query: 645 VAYLEFNANRLLKLIAPHANSIEALTSLIG 734
V L F+++R+LKLI A S++ SLIG
Sbjct: 587 VTGLTFDSDRVLKLIPEWARSVQNWNSLIG 616
>At1g65630.1 68414.m07444 DegP protease, putative contains
similarity to DegP2 protease GI:13172275 from
[Arabidopsis thaliana]
Length = 559
Score = 27.9 bits (59), Expect = 8.8
Identities = 13/42 (30%), Positives = 22/42 (52%)
Frame = +2
Query: 152 LIKKYRYPVEVHKVVTEDGYILEMQRIPHGRDQNTVLTRRNQ 277
LI K +VHKV+ +D IL + +P G D + ++ +
Sbjct: 325 LINKINPLSDVHKVLKKDDIILAIDGVPIGNDSSVHFRKKER 366
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,973,845
Number of Sequences: 28952
Number of extensions: 412292
Number of successful extensions: 1076
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 1030
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1071
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1931371200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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