Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0648
(674 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 152 2e-37
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 152 2e-37
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 152 2e-37
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 152 2e-37
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 100 1e-21
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 94 9e-20
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 68 7e-12
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 66 3e-11
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 40 0.001
At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 40 0.001
At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 38 0.006
At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 38 0.008
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 37 0.014
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 37 0.014
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 34 0.075
At4g11160.1 68417.m01808 translation initiation factor IF-2, mit... 33 0.23
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 33 0.23
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 33 0.23
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 31 0.53
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 31 0.53
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 31 0.70
At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 31 0.70
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 31 0.93
At4g33430.1 68417.m04750 brassinosteroid insensitive 1-associate... 31 0.93
At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 30 1.2
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 30 1.2
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 29 2.1
At5g63930.1 68418.m08028 leucine-rich repeat transmembrane prote... 29 2.8
At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329... 29 3.8
At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329... 29 3.8
At1g62660.1 68414.m07071 beta-fructosidase (BFRUCT3) / beta-fruc... 29 3.8
At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s... 28 5.0
At1g22730.1 68414.m02840 MA3 domain-containing protein contains ... 28 5.0
At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 28 5.0
At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5) M... 28 6.6
At2g28000.1 68415.m03393 RuBisCO subunit binding-protein alpha s... 28 6.6
At5g64600.1 68418.m08118 expressed protein similar to axi 1 [Nic... 27 8.7
At5g06810.1 68418.m00770 mitochondrial transcription termination... 27 8.7
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 152 bits (369), Expect = 2e-37
Identities = 71/84 (84%), Positives = 77/84 (91%)
Frame = +2
Query: 257 IDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQ 436
IDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+GQ
Sbjct: 73 IDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQ 132
Query: 437 TREHALLAFTLGVKQLIVGVNKMD 508
TREHALLAFTLGVKQ+I NKMD
Sbjct: 133 TREHALLAFTLGVKQMICCCNKMD 156
Score = 136 bits (328), Expect = 2e-32
Identities = 64/71 (90%), Positives = 65/71 (91%)
Frame = +3
Query: 42 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 221
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 222 DKLKAERERGI 254
DKLKAERERGI
Sbjct: 61 DKLKAERERGI 71
Score = 81.0 bits (191), Expect = 7e-16
Identities = 33/55 (60%), Positives = 44/55 (80%)
Frame = +1
Query: 508 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWF 672
+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+
Sbjct: 157 ATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 152 bits (369), Expect = 2e-37
Identities = 71/84 (84%), Positives = 77/84 (91%)
Frame = +2
Query: 257 IDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQ 436
IDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+GQ
Sbjct: 73 IDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQ 132
Query: 437 TREHALLAFTLGVKQLIVGVNKMD 508
TREHALLAFTLGVKQ+I NKMD
Sbjct: 133 TREHALLAFTLGVKQMICCCNKMD 156
Score = 136 bits (328), Expect = 2e-32
Identities = 64/71 (90%), Positives = 65/71 (91%)
Frame = +3
Query: 42 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 221
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 222 DKLKAERERGI 254
DKLKAERERGI
Sbjct: 61 DKLKAERERGI 71
Score = 81.0 bits (191), Expect = 7e-16
Identities = 33/55 (60%), Positives = 44/55 (80%)
Frame = +1
Query: 508 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWF 672
+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+
Sbjct: 157 ATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 152 bits (369), Expect = 2e-37
Identities = 71/84 (84%), Positives = 77/84 (91%)
Frame = +2
Query: 257 IDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQ 436
IDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+GQ
Sbjct: 73 IDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQ 132
Query: 437 TREHALLAFTLGVKQLIVGVNKMD 508
TREHALLAFTLGVKQ+I NKMD
Sbjct: 133 TREHALLAFTLGVKQMICCCNKMD 156
Score = 136 bits (328), Expect = 2e-32
Identities = 64/71 (90%), Positives = 65/71 (91%)
Frame = +3
Query: 42 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 221
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 222 DKLKAERERGI 254
DKLKAERERGI
Sbjct: 61 DKLKAERERGI 71
Score = 81.0 bits (191), Expect = 7e-16
Identities = 33/55 (60%), Positives = 44/55 (80%)
Frame = +1
Query: 508 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWF 672
+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+
Sbjct: 157 ATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 152 bits (369), Expect = 2e-37
Identities = 71/84 (84%), Positives = 77/84 (91%)
Frame = +2
Query: 257 IDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQ 436
IDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+GQ
Sbjct: 73 IDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQ 132
Query: 437 TREHALLAFTLGVKQLIVGVNKMD 508
TREHALLAFTLGVKQ+I NKMD
Sbjct: 133 TREHALLAFTLGVKQMICCCNKMD 156
Score = 136 bits (328), Expect = 2e-32
Identities = 64/71 (90%), Positives = 65/71 (91%)
Frame = +3
Query: 42 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 221
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 222 DKLKAERERGI 254
DKLKAERERGI
Sbjct: 61 DKLKAERERGI 71
Score = 81.0 bits (191), Expect = 7e-16
Identities = 33/55 (60%), Positives = 44/55 (80%)
Frame = +1
Query: 508 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWF 672
+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+
Sbjct: 157 ATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 100 bits (239), Expect = 1e-21
Identities = 46/84 (54%), Positives = 59/84 (70%)
Frame = +2
Query: 257 IDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQ 436
+++ FET TI+DAPGH+ ++ NMI+G SQAD VL+++A GEFE G + GQ
Sbjct: 167 VEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKGEFETGYERGGQ 226
Query: 437 TREHALLAFTLGVKQLIVGVNKMD 508
TREH LA TLGV +LIV VNKMD
Sbjct: 227 TREHVQLAKTLGVSKLIVVVNKMD 250
Score = 70.1 bits (164), Expect = 1e-12
Identities = 29/67 (43%), Positives = 48/67 (71%)
Frame = +3
Query: 51 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 230
+K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA++ + S+ A+++D
Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157
Query: 231 KAERERG 251
+ ER +G
Sbjct: 158 EEERLKG 164
Score = 37.1 bits (82), Expect = 0.011
Identities = 17/41 (41%), Positives = 28/41 (68%), Gaps = 1/41 (2%)
Frame = +1
Query: 523 YSEPRFEEIKKEVSSYIKKIGYNPAA-VAFVPISGWHGDNM 642
+S+ R++EI++++ ++K GYN V F+PISG G NM
Sbjct: 256 WSKERYDEIEQKMVPFLKASGYNTKKDVVFLPISGLMGKNM 296
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 93.9 bits (223), Expect = 9e-20
Identities = 43/96 (44%), Positives = 64/96 (66%), Gaps = 1/96 (1%)
Frame = +2
Query: 263 IALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK-NGQT 439
+A+ F + +++V ++D+PGH+DF+ NMI G +QAD A+L++ A G FEAG GQT
Sbjct: 307 VAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEAGFDNLKGQT 366
Query: 440 REHALLAFTLGVKQLIVGVNKMDPLNHHTVSPDLRK 547
REHA + GV+Q+IV +NKMD + + DL K
Sbjct: 367 REHARVLRGFGVEQVIVAINKMDIVGYSKERFDLIK 402
Score = 83.4 bits (197), Expect = 1e-16
Identities = 35/66 (53%), Positives = 49/66 (74%)
Frame = +3
Query: 57 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKA 236
+ +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297
Query: 237 ERERGI 254
ERERGI
Sbjct: 298 ERERGI 303
Score = 33.1 bits (72), Expect = 0.17
Identities = 11/41 (26%), Positives = 26/41 (63%)
Frame = +1
Query: 523 YSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNML 645
YS+ RF+ IK+ V S+++ + +++ ++P+S N++
Sbjct: 393 YSKERFDLIKQHVGSFLQSCRFKDSSLTWIPLSAMENQNLV 433
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 67.7 bits (158), Expect = 7e-12
Identities = 34/87 (39%), Positives = 52/87 (59%)
Frame = +2
Query: 257 IDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQ 436
I+ A ++ET + +D PGH D++KNMITG +Q D A+L+V+ G Q
Sbjct: 130 INTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMP-------Q 182
Query: 437 TREHALLAFTLGVKQLIVGVNKMDPLN 517
T+EH LLA +GV ++V +NK D ++
Sbjct: 183 TKEHILLAKQVGVPDMVVFLNKEDQVD 209
Score = 39.1 bits (87), Expect = 0.003
Identities = 17/45 (37%), Positives = 25/45 (55%)
Frame = +3
Query: 39 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEK 173
K ++K H+NI IGHVD GK+T T L I +K+++
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE 116
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 65.7 bits (153), Expect = 3e-11
Identities = 32/78 (41%), Positives = 48/78 (61%)
Frame = +2
Query: 275 KFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHAL 454
++ET+K + +D PGH D++KNMITG +Q D +L+V+ G QT+EH L
Sbjct: 124 EYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGPMP-------QTKEHIL 176
Query: 455 LAFTLGVKQLIVGVNKMD 508
LA +GV L+ +NK+D
Sbjct: 177 LARQVGVPSLVCFLNKVD 194
Score = 32.7 bits (71), Expect = 0.23
Identities = 12/22 (54%), Positives = 16/22 (72%)
Frame = +3
Query: 48 KEKTHINIVVIGHVDSGKSTTT 113
+ K H+N+ IGHVD GK+T T
Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLT 84
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 40.3 bits (90), Expect = 0.001
Identities = 23/78 (29%), Positives = 39/78 (50%)
Frame = +2
Query: 284 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 463
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 464 TLGVKQLIVGVNKMDPLN 517
+ +K +I+ NK+D +N
Sbjct: 178 MMRLKHIIILQNKIDLIN 195
>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 284
Score = 40.3 bits (90), Expect = 0.001
Identities = 23/78 (29%), Positives = 39/78 (50%)
Frame = +2
Query: 284 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 463
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 464 TLGVKQLIVGVNKMDPLN 517
+ +K +I+ NK+D +N
Sbjct: 178 MMRLKHIIILQNKIDLIN 195
>At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to gb|U37354 from S. pombe. ESTs
gb|T41979, gb|N37284 and gb|N37529 come from this gene
Length = 465
Score = 37.9 bits (84), Expect = 0.006
Identities = 25/81 (30%), Positives = 39/81 (48%), Gaps = 4/81 (4%)
Frame = +2
Query: 278 FETSKY----YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTRE 445
FE SK +V+ +D PGH + M+ G + D A+L++AA QT E
Sbjct: 112 FENSKMKLLRHVSFVDCPGHDILMATMLNGAAIMDGALLLIAANE------TCPQPQTSE 165
Query: 446 HALLAFTLGVKQLIVGVNKMD 508
H + +K +I+ NK+D
Sbjct: 166 HLAAVEIMQLKHIIILQNKID 186
>At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative
Length = 465
Score = 37.5 bits (83), Expect = 0.008
Identities = 22/71 (30%), Positives = 35/71 (49%)
Frame = +2
Query: 296 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGV 475
+V+ +D PGH + M+ G + D A+LI+AA QT EH + +
Sbjct: 120 HVSFVDCPGHDILMATMLNGAAIMDGALLIIAANE------TCPQPQTAEHLASVDMMHL 173
Query: 476 KQLIVGVNKMD 508
K +I+ NK+D
Sbjct: 174 KDIIIIQNKID 184
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 36.7 bits (81), Expect = 0.014
Identities = 19/50 (38%), Positives = 24/50 (48%)
Frame = +2
Query: 254 PIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 403
P+ + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 196 PMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 36.7 bits (81), Expect = 0.014
Identities = 19/50 (38%), Positives = 24/50 (48%)
Frame = +2
Query: 254 PIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 403
P+ + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 196 PMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 34.3 bits (75), Expect = 0.075
Identities = 28/93 (30%), Positives = 42/93 (45%)
Frame = +2
Query: 281 ETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLA 460
E S Y + +ID PGH DF + S A+L+V A G QT + LA
Sbjct: 131 EASGYLLNLIDTPGHVDFSYEVSRSLSACQGALLVVDAAQG-------VQAQTVANFYLA 183
Query: 461 FTLGVKQLIVGVNKMDPLNHHTVSPDLRKSRRK 559
F + ++ +NK+D T P+ K++ K
Sbjct: 184 FEANL-TIVPVINKID---QPTADPERVKAQLK 212
Score = 27.9 bits (59), Expect = 6.6
Identities = 16/40 (40%), Positives = 19/40 (47%)
Frame = +3
Query: 30 D*PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK 149
D K EK N +I H+D GKST L+ G I K
Sbjct: 57 DLTKFPSEKIR-NFSIIAHIDHGKSTLADRLMELTGTIKK 95
>At4g11160.1 68417.m01808 translation initiation factor IF-2,
mitochondrial, putative similar to SP|P46198|IF2M_BOVIN
Translation initiation factor IF-2, mitochondrial
precursor (IF-2Mt) (IF-2(Mt)) {Bos taurus}
Length = 743
Score = 32.7 bits (71), Expect = 0.23
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +2
Query: 299 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 403
+T +D PGH F + G + D VL+VAA G
Sbjct: 270 ITFLDTPGHAAFSEMRARGAAVTDIVVLVVAADDG 304
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 32.7 bits (71), Expect = 0.23
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = +3
Query: 66 NIVVIGHVDSGKSTTTGHLIYKCGG 140
NI ++ HVD GK+T HLI GG
Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG 35
Score = 32.7 bits (71), Expect = 0.23
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +2
Query: 293 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 403
Y + +ID+PGH DF + T +D A+++V A G
Sbjct: 74 YSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEG 110
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 32.7 bits (71), Expect = 0.23
Identities = 12/28 (42%), Positives = 20/28 (71%)
Frame = +3
Query: 54 KTHINIVVIGHVDSGKSTTTGHLIYKCG 137
K + NI ++ H+D+GK+TTT ++Y G
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTG 121
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 31.5 bits (68), Expect = 0.53
Identities = 17/52 (32%), Positives = 25/52 (48%)
Frame = +2
Query: 293 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREH 448
Y V IID PGH DF + D A+L++ + G I+ + Q R +
Sbjct: 133 YKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSITVDRQMRRY 184
Score = 29.1 bits (62), Expect = 2.8
Identities = 21/63 (33%), Positives = 33/63 (52%)
Frame = +3
Query: 66 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 245
NI + H+DSGK+T T +++ G R E E ++ G G+ +D + ERE
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTG----RIHEIHEVRGRD-GVGA-----KMDSMDLERE 116
Query: 246 RGI 254
+GI
Sbjct: 117 KGI 119
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 31.5 bits (68), Expect = 0.53
Identities = 17/52 (32%), Positives = 25/52 (48%)
Frame = +2
Query: 293 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREH 448
Y V IID PGH DF + D A+L++ + G I+ + Q R +
Sbjct: 133 YKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSITVDRQMRRY 184
Score = 29.1 bits (62), Expect = 2.8
Identities = 21/63 (33%), Positives = 33/63 (52%)
Frame = +3
Query: 66 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 245
NI + H+DSGK+T T +++ G R E E ++ G G+ +D + ERE
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTG----RIHEIHEVRGRD-GVGA-----KMDSMDLERE 116
Query: 246 RGI 254
+GI
Sbjct: 117 KGI 119
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 31.1 bits (67), Expect = 0.70
Identities = 22/70 (31%), Positives = 35/70 (50%)
Frame = +2
Query: 305 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 484
+ID PGH F G+S D A+L+V + + G+ QT E +L + +
Sbjct: 705 VIDTPGHESFTNLRSRGSSLCDLAILVV-----DIKHGLEP--QTIE-SLNLLRMRNTEF 756
Query: 485 IVGVNKMDPL 514
I+ +NK+D L
Sbjct: 757 IIALNKVDRL 766
>At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1088
Score = 31.1 bits (67), Expect = 0.70
Identities = 23/72 (31%), Positives = 34/72 (47%)
Frame = +2
Query: 299 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 478
+ +ID PGH F G++ D A+L+V + G+ QT E L VK
Sbjct: 558 ILVIDTPGHESFTNLRSRGSNLCDLAILVV-----DIMRGLEP--QTIESLNLLRRRNVK 610
Query: 479 QLIVGVNKMDPL 514
I+ +NK+D L
Sbjct: 611 -FIIALNKVDRL 621
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 30.7 bits (66), Expect = 0.93
Identities = 13/42 (30%), Positives = 23/42 (54%)
Frame = +2
Query: 278 FETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 403
+E + + + +ID PGH DF + + + A+L+V A G
Sbjct: 147 YEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQG 188
Score = 30.3 bits (65), Expect = 1.2
Identities = 37/124 (29%), Positives = 50/124 (40%), Gaps = 1/124 (0%)
Frame = +3
Query: 66 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 245
N +I H+D GKST L+ G + R + K F LD + ERE
Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDM-----------KEQF-----LDNMDLERE 131
Query: 246 RGIQSILLSGSSKLASTMLP-SLMLLDTEISSRT*SQEPLRLIALCSS*LPVPVNSKLVS 422
RGI L + + P L L+DT S E R +A C L V S+ V
Sbjct: 132 RGITIKLQAARMRYVYEDTPFCLNLIDTP-GHVDFSYEVSRSLAACEGALLVVDASQGVE 190
Query: 423 LRTV 434
+T+
Sbjct: 191 AQTL 194
>At4g33430.1 68417.m04750 brassinosteroid insensitive 1-associated
receptor kinase 1 (BAK1) / somatic embryogenesis
receptor-like kinase 3 (SERK3) identical to SP|Q94F62
BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase
1 precursor (EC 2.7.1.37) (BRI1-associated receptor
kinase 1) (Somatic embryogenesis receptor-like kinase 3)
{Arabidopsis thaliana}; contains Pfam domains PF00560:
Leucine Rich Repeat and PF00069: Protein kinase domain;
identical to cDNA somatic embryogenesis receptor-like
kinase 3 (SERK3) GI:14573458
Length = 615
Score = 30.7 bits (66), Expect = 0.93
Identities = 19/60 (31%), Positives = 28/60 (46%), Gaps = 2/60 (3%)
Frame = -2
Query: 370 ISLRGSCDHVLDEISVSRSINDGNIVLASFELPESN--IDWIPRSRSAFSLSNTQAYLKD 197
+ LRG C + + V + +G++ E PES +DW R R A + AYL D
Sbjct: 347 LRLRGFCMTPTERLLVYPYMANGSVASCLRERPESQPPLDWPKRQRIALGSARGLAYLHD 406
>At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profile PF00009: Elongation
factor Tu GTP binding domain
Length = 630
Score = 30.3 bits (65), Expect = 1.2
Identities = 22/70 (31%), Positives = 34/70 (48%)
Frame = +2
Query: 305 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 484
+ID PGH F G+S D A+L+V + G+ QT E +L + +
Sbjct: 113 VIDTPGHESFTNLRSRGSSLCDLAILVV-----DITHGLQP--QTIE-SLNLLRMRNTEF 164
Query: 485 IVGVNKMDPL 514
I+ +NK+D L
Sbjct: 165 IIALNKVDRL 174
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 30.3 bits (65), Expect = 1.2
Identities = 23/70 (32%), Positives = 34/70 (48%)
Frame = +2
Query: 305 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 484
+ID PGH F G+S D A+L+V + G+ QT E +L + +
Sbjct: 772 VIDTPGHESFTNLRSRGSSLCDLAILVV-----DIMHGLEP--QTIE-SLNLLRMRNTEF 823
Query: 485 IVGVNKMDPL 514
IV +NK+D L
Sbjct: 824 IVALNKVDRL 833
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 29.5 bits (63), Expect = 2.1
Identities = 11/34 (32%), Positives = 20/34 (58%)
Frame = +2
Query: 287 SKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 388
++Y + +ID+PGH DF + D A+++V
Sbjct: 96 NEYLINLIDSPGHVDFSSEVTAALRITDGALVVV 129
Score = 29.1 bits (62), Expect = 2.8
Identities = 13/24 (54%), Positives = 15/24 (62%)
Frame = +3
Query: 66 NIVVIGHVDSGKSTTTGHLIYKCG 137
N+ VI HVD GKST T L+ G
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAG 44
>At5g63930.1 68418.m08028 leucine-rich repeat transmembrane protein
kinase, putative
Length = 1102
Score = 29.1 bits (62), Expect = 2.8
Identities = 19/75 (25%), Positives = 32/75 (42%), Gaps = 2/75 (2%)
Frame = -2
Query: 421 DTSF--EFTGTGSYDEHSAISLRGSCDHVLDEISVSRSINDGNIVLASFELPESNIDWIP 248
D SF E G+ + + L G C+H + + + G++ + P N+DW
Sbjct: 847 DNSFRAEILTLGNIRHRNIVKLHGFCNHQGSNLLLYEYMPKGSLGEILHD-PSCNLDWSK 905
Query: 247 RSRSAFSLSNTQAYL 203
R + A + AYL
Sbjct: 906 RFKIALGAAQGLAYL 920
>At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 442
Score = 28.7 bits (61), Expect = 3.8
Identities = 15/40 (37%), Positives = 22/40 (55%)
Frame = +1
Query: 502 NGSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 621
N +TE + P+ E K E+S++I IG+ V F P S
Sbjct: 61 NVATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
>At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 440
Score = 28.7 bits (61), Expect = 3.8
Identities = 15/40 (37%), Positives = 22/40 (55%)
Frame = +1
Query: 502 NGSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 621
N +TE + P+ E K E+S++I IG+ V F P S
Sbjct: 61 NVATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
>At1g62660.1 68414.m07071 beta-fructosidase (BFRUCT3) /
beta-fructofuranosidase / invertase, vacuolar identical
to beta-fructosidase GB:CAA67560 GI:1429209 [Arabidopsis
thaliana]; supported by full-length cDNA GI:14517549;
identical to cDNA Beta-fructosidase GI:3115854
Length = 648
Score = 28.7 bits (61), Expect = 3.8
Identities = 17/54 (31%), Positives = 24/54 (44%), Gaps = 1/54 (1%)
Frame = -2
Query: 538 IWAHCMVVQWIHFVYSYDELFDTEGESEQGMLTG-LTVLRDTSFEFTGTGSYDE 380
+W H + IH++Y + + G+ TG T L D S TGS DE
Sbjct: 148 VWGHAVSKDLIHWLYLPIAMVPDQWYDANGVWTGSATFLDDGSIVMLYTGSTDE 201
>At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein
similar to 1,4-alpha-glucan branching enzyme [Solanum
tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme
(EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum
tuberosum} SP|P30924; contains Pfam profiles: PF00128
Alpha amylase catalytic domain, PF02922 Isoamylase
N-terminal domain
Length = 777
Score = 28.3 bits (60), Expect = 5.0
Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 3/33 (9%)
Frame = +1
Query: 526 SEPR---FEEIKKEVSSYIKKIGYNPAAVAFVP 615
SEP+ FEE K+V ++K+ GYN + VP
Sbjct: 257 SEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVP 289
>At1g22730.1 68414.m02840 MA3 domain-containing protein contains
Pfam profile PF02847: MA3 domain; low similarity to
programmed cell death 4 protein [Gallus gallus]
GI:12958564
Length = 693
Score = 28.3 bits (60), Expect = 5.0
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +2
Query: 467 LGVKQLIVGVNKMDPLNHHTVSPDLRKSRRKYP 565
L + QL + + +DPL ++ DL KSRR P
Sbjct: 14 LCISQLKISSSSLDPLPQANMAEDLTKSRRHSP 46
>At1g17220.1 68414.m02098 translation initiation factor IF-2,
chloroplast, putative similar to SP|P57997|IF2C_PHAVU
Translation initiation factor IF-2, chloroplast
precursor (PvIF2cp) {Phaseolus vulgaris}
Length = 1026
Score = 28.3 bits (60), Expect = 5.0
Identities = 19/67 (28%), Positives = 29/67 (43%)
Frame = +2
Query: 308 IDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLI 487
+D PGH F G D A+++VAA G QT E A+ ++
Sbjct: 557 LDTPGHEAFGAMRARGARVTDIAIIVVAADDG-------IRPQTNE-AIAHAKAAAVPIV 608
Query: 488 VGVNKMD 508
+ +NK+D
Sbjct: 609 IAINKID 615
>At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5)
Monovalent cation:proton antiporter family 2 (CPA2
family) member, PMID:11500563; related to
glutathione-regulated potassium-efflux system protein
[Escherichia coli] GP|606284|gb|AAA58147
Length = 568
Score = 27.9 bits (59), Expect = 6.6
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +1
Query: 532 PRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNM 642
PRF ++ ++SS ++ Y AAVAF +S W D +
Sbjct: 354 PRFLKLMIQLSSQTNEL-YQLAAVAFCLLSAWCSDKL 389
>At2g28000.1 68415.m03393 RuBisCO subunit binding-protein alpha
subunit, chloroplast / 60 kDa chaperonin alpha subunit /
CPN-60 alpha identical to SWISS-PROT:P21238- RuBisCO
subunit binding-protein alpha subunit, chloroplast
precursor (60 kDa chaperonin alpha subunit, CPN-60
alpha) [Arabidopsis thaliana]
Length = 586
Score = 27.9 bits (59), Expect = 6.6
Identities = 16/58 (27%), Positives = 29/58 (50%)
Frame = -2
Query: 391 SYDEHSAISLRGSCDHVLDEISVSRSINDGNIVLASFELPESNIDWIPRSRSAFSLSN 218
++D+HS +L+ D + D + ++ N+VL F P+ D + +R A L N
Sbjct: 52 AFDQHSRAALQAGIDKLADCVGLTLGPRGRNVVLDEFGSPKVVNDGVTIAR-AIELPN 108
>At5g64600.1 68418.m08118 expressed protein similar to axi 1
[Nicotiana tabacum] GI:559921; contains Pfam profile
PF03138: Plant protein family
Length = 522
Score = 27.5 bits (58), Expect = 8.7
Identities = 19/68 (27%), Positives = 32/68 (47%), Gaps = 2/68 (2%)
Frame = +1
Query: 469 RCQTAHRRSKQNGSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHG--DNM 642
R T+H + K STE E KEV ++K +GY+ + V ++ +G D +
Sbjct: 313 RESTSHWKIKSINSTEQR-EEGLCPLTPKEVGIFLKGLGYSQSTVIYIAAGEIYGGDDRL 371
Query: 643 LEPSTKMP 666
E ++ P
Sbjct: 372 SELKSRFP 379
>At5g06810.1 68418.m00770 mitochondrial transcription termination
factor-related / mTERF-related contains Pfam profile
PF02536: mTERF
Length = 1141
Score = 27.5 bits (58), Expect = 8.7
Identities = 16/52 (30%), Positives = 27/52 (51%)
Frame = +3
Query: 117 HLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGIQSILLS 272
H + G+D + I K KEA+E+ S++ + K+KA + G + LS
Sbjct: 174 HHVLCYSGVDPKRIGKIFKEAREV--FSYETGVLASKIKAYEDLGFSRLFLS 223
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,306,234
Number of Sequences: 28952
Number of extensions: 325495
Number of successful extensions: 1137
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 1065
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1130
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1432596384
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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