BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0644 (633 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23C4.19 |spt5||transcription elongation factor Spt5|Schizosa... 30 0.24 SPAC19B12.06c |||rhomboid family protease|Schizosaccharomyces po... 26 5.2 SPAC27F1.07 |||dolichyl-diphospho-oligosaccharide-protein glycos... 25 6.9 >SPAC23C4.19 |spt5||transcription elongation factor Spt5|Schizosaccharomyces pombe|chr 1|||Manual Length = 990 Score = 30.3 bits (65), Expect = 0.24 Identities = 23/74 (31%), Positives = 35/74 (47%), Gaps = 1/74 (1%) Frame = -3 Query: 235 DESVEVS-SDSFTVIVYREDGAAXXGERRSHNRVDRQQQRFTDLRASPMKEVKYNIPFSQ 59 DE E+ D F + E GA G+ R H +DRQ+Q + A + E +Y + + Sbjct: 162 DEEDEIGREDGF---IEEEVGADYVGDDRRHRELDRQRQELQSVDAERLAE-EYREKYGR 217 Query: 58 IETVRCSSSAQ*QR 17 +TV +S QR Sbjct: 218 SQTVVGDTSNVPQR 231 >SPAC19B12.06c |||rhomboid family protease|Schizosaccharomyces pombe|chr 1|||Manual Length = 258 Score = 25.8 bits (54), Expect = 5.2 Identities = 14/34 (41%), Positives = 19/34 (55%), Gaps = 1/34 (2%) Frame = +3 Query: 534 PDDANIVEVLHTTAGLIGYDYPLGDLDFY-PSGG 632 P D +I VLH TA +I + +DF+ P GG Sbjct: 178 PLDFSITIVLHLTAVVISIIFSFAYMDFFLPRGG 211 >SPAC27F1.07 |||dolichyl-diphospho-oligosaccharide-protein glycosyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 450 Score = 25.4 bits (53), Expect = 6.9 Identities = 13/37 (35%), Positives = 24/37 (64%), Gaps = 2/37 (5%) Frame = +1 Query: 214 WKL--QRFRHTRSPRREDVNVIAVDWSPGSKLYNEGL 318 WK+ + FR +++ RRE ++ I + WSPG +Y + + Sbjct: 325 WKMPYEEFRSSKN-RREYLD-IPLAWSPGDMIYEKAV 359 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,399,565 Number of Sequences: 5004 Number of extensions: 45914 Number of successful extensions: 109 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 107 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 109 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 281707720 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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