BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0644
(633 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23C4.19 |spt5||transcription elongation factor Spt5|Schizosa... 30 0.24
SPAC19B12.06c |||rhomboid family protease|Schizosaccharomyces po... 26 5.2
SPAC27F1.07 |||dolichyl-diphospho-oligosaccharide-protein glycos... 25 6.9
>SPAC23C4.19 |spt5||transcription elongation factor
Spt5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 990
Score = 30.3 bits (65), Expect = 0.24
Identities = 23/74 (31%), Positives = 35/74 (47%), Gaps = 1/74 (1%)
Frame = -3
Query: 235 DESVEVS-SDSFTVIVYREDGAAXXGERRSHNRVDRQQQRFTDLRASPMKEVKYNIPFSQ 59
DE E+ D F + E GA G+ R H +DRQ+Q + A + E +Y + +
Sbjct: 162 DEEDEIGREDGF---IEEEVGADYVGDDRRHRELDRQRQELQSVDAERLAE-EYREKYGR 217
Query: 58 IETVRCSSSAQ*QR 17
+TV +S QR
Sbjct: 218 SQTVVGDTSNVPQR 231
>SPAC19B12.06c |||rhomboid family protease|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 258
Score = 25.8 bits (54), Expect = 5.2
Identities = 14/34 (41%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
Frame = +3
Query: 534 PDDANIVEVLHTTAGLIGYDYPLGDLDFY-PSGG 632
P D +I VLH TA +I + +DF+ P GG
Sbjct: 178 PLDFSITIVLHLTAVVISIIFSFAYMDFFLPRGG 211
>SPAC27F1.07 |||dolichyl-diphospho-oligosaccharide-protein
glycosyltransferase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 450
Score = 25.4 bits (53), Expect = 6.9
Identities = 13/37 (35%), Positives = 24/37 (64%), Gaps = 2/37 (5%)
Frame = +1
Query: 214 WKL--QRFRHTRSPRREDVNVIAVDWSPGSKLYNEGL 318
WK+ + FR +++ RRE ++ I + WSPG +Y + +
Sbjct: 325 WKMPYEEFRSSKN-RREYLD-IPLAWSPGDMIYEKAV 359
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,399,565
Number of Sequences: 5004
Number of extensions: 45914
Number of successful extensions: 109
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 107
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 109
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 281707720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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