BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0632 (719 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 125 7e-30 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 125 7e-30 SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 97 3e-21 SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 75 1e-14 SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 33 0.041 SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein ... 27 3.6 SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces pombe... 27 3.6 SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit 1|Schizosacchar... 25 8.2 SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomy... 25 8.2 SPAC11D3.15 |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||M... 25 8.2 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 125 bits (301), Expect = 7e-30 Identities = 57/84 (67%), Positives = 66/84 (78%) Frame = +1 Query: 256 DAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVF 435 DAIHRGGGQIIPT RR +YA L A P + EPV+L EIQ E A+GGIY VLN++RGHVF Sbjct: 696 DAIHRGGGQIIPTARRVVYASTLLASPIIQEPVFLVEIQVSENAMGGIYSVLNKKRGHVF 755 Query: 436 EESQVAGTPMFIVKAYLPVNESFG 507 E Q GTP++ +KAYLPVNESFG Sbjct: 756 SEEQRVGTPLYNIKAYLPVNESFG 779 Score = 124 bits (299), Expect = 1e-29 Identities = 55/84 (65%), Positives = 66/84 (78%) Frame = +2 Query: 2 DFKTRARYLTEKYEYDVTEARKIWCFGPEGTAPNILVDCSKGVQYLNEIKDSVVAGFQWA 181 DFK RAR + +++ +DVT+ARKIWCFGP+ T N++VD +K V YLNEIKDSVVA F WA Sbjct: 611 DFKVRARIMADEFGWDVTDARKIWCFGPDTTGANVVVDQTKAVAYLNEIKDSVVAAFAWA 670 Query: 182 AKEGVMAEENLRGVRFNIYDVTLH 253 +KEG M EENLR RFNI DV LH Sbjct: 671 SKEGPMFEENLRSCRFNILDVVLH 694 Score = 91.5 bits (217), Expect = 1e-19 Identities = 39/63 (61%), Positives = 46/63 (73%) Frame = +3 Query: 507 FTADLRSNTGGQAFPQCVFDHWQVLPGDPCEPQSKPYNVVQETRKRKGLKEGLPDLTQYL 686 FT +LR T GQAFPQ VFDHW + GDP +P SKP +V E RKRKGLKE +PD T+Y Sbjct: 780 FTGELRQATAGQAFPQLVFDHWSPMSGDPLDPTSKPGQIVCEARKRKGLKENVPDYTEYY 839 Query: 687 DKL 695 D+L Sbjct: 840 DRL 842 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 125 bits (301), Expect = 7e-30 Identities = 57/84 (67%), Positives = 66/84 (78%) Frame = +1 Query: 256 DAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVF 435 DAIHRGGGQIIPT RR +YA L A P + EPV+L EIQ E A+GGIY VLN++RGHVF Sbjct: 696 DAIHRGGGQIIPTARRVVYASTLLASPIIQEPVFLVEIQVSENAMGGIYSVLNKKRGHVF 755 Query: 436 EESQVAGTPMFIVKAYLPVNESFG 507 E Q GTP++ +KAYLPVNESFG Sbjct: 756 SEEQRVGTPLYNIKAYLPVNESFG 779 Score = 124 bits (299), Expect = 1e-29 Identities = 55/84 (65%), Positives = 66/84 (78%) Frame = +2 Query: 2 DFKTRARYLTEKYEYDVTEARKIWCFGPEGTAPNILVDCSKGVQYLNEIKDSVVAGFQWA 181 DFK RAR + +++ +DVT+ARKIWCFGP+ T N++VD +K V YLNEIKDSVVA F WA Sbjct: 611 DFKVRARIMADEFGWDVTDARKIWCFGPDTTGANVVVDQTKAVAYLNEIKDSVVAAFAWA 670 Query: 182 AKEGVMAEENLRGVRFNIYDVTLH 253 +KEG M EENLR RFNI DV LH Sbjct: 671 SKEGPMFEENLRSCRFNILDVVLH 694 Score = 91.5 bits (217), Expect = 1e-19 Identities = 39/63 (61%), Positives = 46/63 (73%) Frame = +3 Query: 507 FTADLRSNTGGQAFPQCVFDHWQVLPGDPCEPQSKPYNVVQETRKRKGLKEGLPDLTQYL 686 FT +LR T GQAFPQ VFDHW + GDP +P SKP +V E RKRKGLKE +PD T+Y Sbjct: 780 FTGELRQATAGQAFPQLVFDHWSPMSGDPLDPTSKPGQIVCEARKRKGLKENVPDYTEYY 839 Query: 687 DKL 695 D+L Sbjct: 840 DRL 842 >SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 983 Score = 96.7 bits (230), Expect = 3e-21 Identities = 43/84 (51%), Positives = 60/84 (71%) Frame = +1 Query: 256 DAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVF 435 + I+RGGGQIIPT RR Y+ LTA PRLMEPVY+ E+ P ++ IY +L RRRGHV Sbjct: 812 EQIYRGGGQIIPTARRVCYSSFLTASPRLMEPVYMVEVHAPADSLPIIYDLLTRRRGHVL 871 Query: 436 EESQVAGTPMFIVKAYLPVNESFG 507 ++ G+P+++V+A +PV +S G Sbjct: 872 QDIPRPGSPLYLVRALIPVIDSCG 895 Score = 65.7 bits (153), Expect = 6e-12 Identities = 29/85 (34%), Positives = 46/85 (54%), Gaps = 4/85 (4%) Frame = +2 Query: 8 KTRARYLTEKYEYDVTEARKIWCFGPEGTAPNILVDCSKGVQY----LNEIKDSVVAGFQ 175 K + + + Y++D+ +R IW FGP+ NIL D + LN +K+ + GFQ Sbjct: 725 KRISEFFQKNYDWDLLASRSIWAFGPDDRGTNILRDDTLSTDVDKNVLNSVKEYIKQGFQ 784 Query: 176 WAAKEGVMAEENLRGVRFNIYDVTL 250 W +EG + +E +R V F + DV L Sbjct: 785 WGTREGPLCDETIRNVNFRLMDVVL 809 Score = 54.8 bits (126), Expect = 1e-08 Identities = 32/74 (43%), Positives = 42/74 (56%), Gaps = 12/74 (16%) Frame = +3 Query: 507 FTADLRSNTGGQAFPQCVFDHWQVLPGDPCEPQSKP------------YNVVQETRKRKG 650 F DLR +T GQA Q VFDHWQV+PGDP + KP + + +TR+RKG Sbjct: 896 FETDLRVHTQGQAMCQMVFDHWQVVPGDPLDKSIKPKPLEPARGSDLARDFLIKTRRRKG 955 Query: 651 LKEGLPDLTQYLDK 692 L E + T+Y D+ Sbjct: 956 LVEDV-STTRYFDQ 968 >SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1000 Score = 74.5 bits (175), Expect = 1e-14 Identities = 34/77 (44%), Positives = 47/77 (61%) Frame = +1 Query: 277 GQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAG 456 GQ+I + + L PRLM +Y C++Q +G +YGV+++RRG V +E G Sbjct: 841 GQVISVVKESIRHGFLGWSPRLMLAMYSCDVQATSEVLGRVYGVVSKRRGRVIDEEMKEG 900 Query: 457 TPMFIVKAYLPVNESFG 507 TP FIVKA +PV ESFG Sbjct: 901 TPFFIVKALIPVVESFG 917 Score = 27.9 bits (59), Expect = 1.5 Identities = 24/92 (26%), Positives = 41/92 (44%), Gaps = 15/92 (16%) Frame = +2 Query: 5 FKTRARYLTEKYEYDVTEARK----IWCFGPEGTAPNILVDCSKGVQYL----------- 139 F R L E+ D+ E + I FGP+ PNIL D +K ++ Sbjct: 726 FYERLSKLLEEENSDLGELKNHLDSIIAFGPKRVGPNILFDKTKKMRDFRRQSDETKLIP 785 Query: 140 NEIKDSVVAGFQWAAKEGVMAEENLRGVRFNI 235 +++ + VV FQ +G + E ++G+ +I Sbjct: 786 SDLSEYVVTAFQLITHQGPLCAEPVQGICVSI 817 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 33.1 bits (72), Expect = 0.041 Identities = 17/61 (27%), Positives = 30/61 (49%) Frame = +1 Query: 322 LTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNES 501 L A P ++EP+ I P GG+ G L++R+ + + F ++A +P+N Sbjct: 668 LQANPMVLEPIMNVSITAPVEHQGGVIGNLDKRKATIVDSD--TDEDEFTLQAEVPLNSM 725 Query: 502 F 504 F Sbjct: 726 F 726 >SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein Lsb1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 296 Score = 26.6 bits (56), Expect = 3.6 Identities = 18/58 (31%), Positives = 27/58 (46%) Frame = +2 Query: 134 YLNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHMMPSIEVVAKSFQQLEDA 307 YLN + + FQ+ EGV+ L V NI+ L P+ V A + Q +E + Sbjct: 6 YLNHVIRGIYNDFQFLVDEGVVERSALDWVHANIH---LQDGPASPVTAPAAQPVESS 60 >SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces pombe|chr 2|||Manual Length = 230 Score = 26.6 bits (56), Expect = 3.6 Identities = 11/26 (42%), Positives = 16/26 (61%) Frame = -2 Query: 88 LGAKAPNLTGFGNIIFVLFCKIPSAS 11 +G PNL GN +F L ++PS+S Sbjct: 169 IGILPPNLNSIGNKMFSLKSRVPSSS 194 >SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit 1|Schizosaccharomyces pombe|chr 3|||Manual Length = 232 Score = 25.4 bits (53), Expect = 8.2 Identities = 12/27 (44%), Positives = 14/27 (51%) Frame = +3 Query: 567 HWQVLPGDPCEPQSKPYNVVQETRKRK 647 H+Q L GDP + PY VQ RK Sbjct: 78 HFQNLSGDPVSQMATPYPPVQIELMRK 104 >SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomyces pombe|chr 2|||Manual Length = 542 Score = 25.4 bits (53), Expect = 8.2 Identities = 17/67 (25%), Positives = 29/67 (43%), Gaps = 1/67 (1%) Frame = +1 Query: 286 IPTTRRCLYACLLTAQPRLMEPVYL-CEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTP 462 IP T RC+ + + + P+L P L + CP + + V+ H F + P Sbjct: 386 IPNTERCILSAFICSPPQLPLPNPLRMYLPCPSLNSTEV-SVITLAPQHSFLNIVINLNP 444 Query: 463 MFIVKAY 483 +K+Y Sbjct: 445 ALALKSY 451 >SPAC11D3.15 |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1317 Score = 25.4 bits (53), Expect = 8.2 Identities = 11/31 (35%), Positives = 18/31 (58%) Frame = +2 Query: 134 YLNEIKDSVVAGFQWAAKEGVMAEENLRGVR 226 YL+ + +AGFQ G+ ++N +GVR Sbjct: 248 YLSPVVRRYLAGFQSGFLHGLKTKDNSKGVR 278 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,312,324 Number of Sequences: 5004 Number of extensions: 75105 Number of successful extensions: 202 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 187 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 201 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 337208592 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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