Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0632
(719 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 133 1e-31
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 103 9e-23
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 103 9e-23
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 101 5e-22
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 68 5e-12
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 42 5e-04
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 42 5e-04
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 39 0.003
At5g13650.2 68418.m01585 elongation factor family protein contai... 35 0.047
At5g13650.1 68418.m01584 elongation factor family protein contai... 35 0.047
At2g03500.1 68415.m00309 myb family transcription factor contain... 31 0.58
At3g49750.1 68416.m05439 leucine-rich repeat family protein cont... 31 1.0
At5g03415.1 68418.m00294 DPB-1 transcription factor, putative (D... 30 1.3
At5g61930.1 68418.m07774 expressed protein contains Pfam PF05634... 29 3.1
At2g35490.1 68415.m04347 plastid-lipid associated protein PAP, p... 29 3.1
At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /... 27 9.5
At4g14990.1 68417.m02303 expressed protein 27 9.5
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 133 bits (321), Expect = 1e-31
Identities = 59/84 (70%), Positives = 71/84 (84%)
Frame = +1
Query: 256 DAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVF 435
DAIHRGGGQ+IPT RR +YA +TA+PRL+EPVY+ EIQ PE A+GGIY VLN++RGHVF
Sbjct: 697 DAIHRGGGQVIPTARRVIYASQITAKPRLLEPVYMVEIQAPEGALGGIYSVLNQKRGHVF 756
Query: 436 EESQVAGTPMFIVKAYLPVNESFG 507
EE Q GTP++ +KAYLPV ESFG
Sbjct: 757 EEMQRPGTPLYNIKAYLPVVESFG 780
Score = 119 bits (286), Expect = 2e-27
Identities = 53/84 (63%), Positives = 66/84 (78%)
Frame = +2
Query: 2 DFKTRARYLTEKYEYDVTEARKIWCFGPEGTAPNILVDCSKGVQYLNEIKDSVVAGFQWA 181
D K R++ L E++ +D A+KIW FGPE T PN++VD KGVQYLNEIKDSVVAGFQWA
Sbjct: 612 DPKIRSKILAEEFGWDKDLAKKIWAFGPETTGPNMVVDMCKGVQYLNEIKDSVVAGFQWA 671
Query: 182 AKEGVMAEENLRGVRFNIYDVTLH 253
+KEG +AEEN+RG+ F + DV LH
Sbjct: 672 SKEGPLAEENMRGICFEVCDVVLH 695
Score = 79.4 bits (187), Expect = 2e-15
Identities = 33/63 (52%), Positives = 47/63 (74%)
Frame = +3
Query: 507 FTADLRSNTGGQAFPQCVFDHWQVLPGDPCEPQSKPYNVVQETRKRKGLKEGLPDLTQYL 686
F++ LR+ T GQAFPQCVFDHW+++ DP EP ++ +V + RKRKGLKE + L+++
Sbjct: 781 FSSQLRAATSGQAFPQCVFDHWEMMSSDPLEPGTQASVLVADIRKRKGLKEAMTPLSEFE 840
Query: 687 DKL 695
DKL
Sbjct: 841 DKL 843
>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 103 bits (248), Expect = 9e-23
Identities = 50/84 (59%), Positives = 58/84 (69%)
Frame = +1
Query: 256 DAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVF 435
+ +HRG GQ+IPT RR Y+ L A PRLMEPVY EIQ P V IY VL+RRRGHV
Sbjct: 813 EPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVT 872
Query: 436 EESQVAGTPMFIVKAYLPVNESFG 507
+ GTP +IVKA+LPV ESFG
Sbjct: 873 SDVPQPGTPAYIVKAFLPVIESFG 896
Score = 73.7 bits (173), Expect = 1e-13
Identities = 33/84 (39%), Positives = 50/84 (59%), Gaps = 4/84 (4%)
Frame = +2
Query: 23 YLTEKYEYDVTEARKIWCFGPEGTAPNILVDCSKGVQY----LNEIKDSVVAGFQWAAKE 190
+ KY++D+ AR IW FGP+ PNIL+D + + + +KDS+V GFQW A+E
Sbjct: 731 FFRTKYDWDLLAARSIWAFGPDKQGPNILLDDTLPTEVDRNLMMAVKDSIVQGFQWGARE 790
Query: 191 GVMAEENLRGVRFNIYDVTLHMMP 262
G + +E +R V+F I D + P
Sbjct: 791 GPLCDEPIRNVKFKIVDARIAPEP 814
Score = 46.0 bits (104), Expect = 3e-05
Identities = 26/63 (41%), Positives = 35/63 (55%), Gaps = 12/63 (19%)
Frame = +3
Query: 507 FTADLRSNTGGQAFPQCVFDHWQVLPGDPCEP--QSKPY----------NVVQETRKRKG 650
F DLR +T GQAF VFDHW ++PGDP + Q +P + +TR+RKG
Sbjct: 897 FETDLRYHTQGQAFCLSVFDHWAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRKG 956
Query: 651 LKE 659
+ E
Sbjct: 957 MSE 959
>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 103 bits (248), Expect = 9e-23
Identities = 50/84 (59%), Positives = 58/84 (69%)
Frame = +1
Query: 256 DAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVF 435
+ +HRG GQ+IPT RR Y+ L A PRLMEPVY EIQ P V IY VL+RRRGHV
Sbjct: 813 EPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVT 872
Query: 436 EESQVAGTPMFIVKAYLPVNESFG 507
+ GTP +IVKA+LPV ESFG
Sbjct: 873 SDVPQPGTPAYIVKAFLPVIESFG 896
Score = 73.7 bits (173), Expect = 1e-13
Identities = 33/84 (39%), Positives = 50/84 (59%), Gaps = 4/84 (4%)
Frame = +2
Query: 23 YLTEKYEYDVTEARKIWCFGPEGTAPNILVDCSKGVQY----LNEIKDSVVAGFQWAAKE 190
+ KY++D+ AR IW FGP+ PNIL+D + + + +KDS+V GFQW A+E
Sbjct: 731 FFRTKYDWDLLAARSIWAFGPDKQGPNILLDDTLPTEVDRNLMMAVKDSIVQGFQWGARE 790
Query: 191 GVMAEENLRGVRFNIYDVTLHMMP 262
G + +E +R V+F I D + P
Sbjct: 791 GPLCDEPIRNVKFKIVDARIAPEP 814
Score = 46.0 bits (104), Expect = 3e-05
Identities = 26/63 (41%), Positives = 35/63 (55%), Gaps = 12/63 (19%)
Frame = +3
Query: 507 FTADLRSNTGGQAFPQCVFDHWQVLPGDPCEP--QSKPY----------NVVQETRKRKG 650
F DLR +T GQAF VFDHW ++PGDP + Q +P + +TR+RKG
Sbjct: 897 FETDLRYHTQGQAFCLSVFDHWAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRKG 956
Query: 651 LKE 659
+ E
Sbjct: 957 MSE 959
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 101 bits (242), Expect = 5e-22
Identities = 49/84 (58%), Positives = 58/84 (69%)
Frame = +1
Query: 256 DAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVF 435
+ +HRG GQ+IPT RR Y+ L A PRLMEPVY EIQ P V IY VL+RRRG+V
Sbjct: 799 EPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGYVT 858
Query: 436 EESQVAGTPMFIVKAYLPVNESFG 507
+ GTP +IVKA+LPV ESFG
Sbjct: 859 SDVPQPGTPAYIVKAFLPVIESFG 882
Score = 70.1 bits (164), Expect = 1e-12
Identities = 32/84 (38%), Positives = 49/84 (58%), Gaps = 4/84 (4%)
Frame = +2
Query: 23 YLTEKYEYDVTEARKIWCFGPEGTAPNILVDCSKGVQY----LNEIKDSVVAGFQWAAKE 190
+ KY++D+ AR IW FGP+ NIL+D + + + +KDS+V GFQW A+E
Sbjct: 717 FFRTKYDWDLLAARSIWAFGPDKQGTNILLDDTLPTEVDRNLMMGVKDSIVQGFQWGARE 776
Query: 191 GVMAEENLRGVRFNIYDVTLHMMP 262
G + +E +R V+F I D + P
Sbjct: 777 GPLCDEPIRNVKFKIVDARIAPEP 800
Score = 46.0 bits (104), Expect = 3e-05
Identities = 26/63 (41%), Positives = 35/63 (55%), Gaps = 12/63 (19%)
Frame = +3
Query: 507 FTADLRSNTGGQAFPQCVFDHWQVLPGDPCEP--QSKPY----------NVVQETRKRKG 650
F DLR +T GQAF VFDHW ++PGDP + Q +P + +TR+RKG
Sbjct: 883 FETDLRYHTQGQAFCLSVFDHWAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRKG 942
Query: 651 LKE 659
+ E
Sbjct: 943 MSE 945
>At3g22980.1 68416.m02898 elongation factor Tu family protein similar
to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 68.1 bits (159), Expect = 5e-12
Identities = 29/77 (37%), Positives = 46/77 (59%)
Frame = +1
Query: 277 GQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAG 456
GQ++ + A +L PR++E +Y CE+ +G +Y VL+RRR + +E G
Sbjct: 853 GQVMTAVKDACRAAVLQTNPRIVEAMYFCELNTAPEYLGPMYAVLSRRRARILKEEMQEG 912
Query: 457 TPMFIVKAYLPVNESFG 507
+ +F V AY+PV+ESFG
Sbjct: 913 SSLFTVHAYVPVSESFG 929
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 41.5 bits (93), Expect = 5e-04
Identities = 20/64 (31%), Positives = 34/64 (53%)
Frame = +1
Query: 316 CLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVN 495
C A+P ++EPV L E++ P G + G +N+R+G + Q ++ A +P+N
Sbjct: 649 CYTAARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLN 706
Query: 496 ESFG 507
FG
Sbjct: 707 NMFG 710
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 41.5 bits (93), Expect = 5e-04
Identities = 20/64 (31%), Positives = 34/64 (53%)
Frame = +1
Query: 316 CLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVN 495
C A+P ++EPV L E++ P G + G +N+R+G + Q ++ A +P+N
Sbjct: 649 CYTAARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLN 706
Query: 496 ESFG 507
FG
Sbjct: 707 NMFG 710
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 39.1 bits (87), Expect = 0.003
Identities = 20/59 (33%), Positives = 32/59 (54%)
Frame = +1
Query: 328 AQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESF 504
A PR++EP+ E+ PE +G + G LN RRG + G + +V + +P+ E F
Sbjct: 683 AGPRMLEPIMRVEVVTPEEHLGDVIGDLNSRRGQINSFGDKPG-GLKVVDSLVPLAEMF 740
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 35.1 bits (77), Expect = 0.047
Identities = 15/58 (25%), Positives = 29/58 (50%)
Frame = +1
Query: 337 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGL 510
+L+EP + ++ PE +G + +L +RRG +F+ V ++ +P GL
Sbjct: 473 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGL 530
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 35.1 bits (77), Expect = 0.047
Identities = 15/58 (25%), Positives = 29/58 (50%)
Frame = +1
Query: 337 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGL 510
+L+EP + ++ PE +G + +L +RRG +F+ V ++ +P GL
Sbjct: 472 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGL 529
>At2g03500.1 68415.m00309 myb family transcription factor contains
Pfam profile: PF00249 myb-like DNA-binding domain
Length = 432
Score = 31.5 bits (68), Expect = 0.58
Identities = 15/39 (38%), Positives = 23/39 (58%), Gaps = 3/39 (7%)
Frame = -1
Query: 305 HLLVVGMIWPPPLWMASYG---VLHHRC*I*HHANSLQP 198
HL+V+G IW PP + +++G L+H HH N+ P
Sbjct: 305 HLVVLGGIWVPPEYTSAHGGTPTLYHHQVHHHHTNTAGP 343
>At3g49750.1 68416.m05439 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to resistance
gene Hcr2-5B, Lycopersicon esculentum, EMBL:AF053997
Length = 274
Score = 30.7 bits (66), Expect = 1.0
Identities = 25/74 (33%), Positives = 36/74 (48%), Gaps = 9/74 (12%)
Frame = -3
Query: 375 TLNFTKIYRLHKTGLSS*QTCVQASSSCWN----DLATTSMDGII-----WSVTS*MLNL 223
T N +IY+L T LS + S+C N DL++ + G+I + V +LNL
Sbjct: 69 TCNNGRIYKLSLTNLSLRGSISPFLSNCTNLQSLDLSSNQISGVIPPEIQYLVNLAVLNL 128
Query: 222 TPRKFSSAITPSLA 181
+ S ITP LA
Sbjct: 129 SSNHLSGEITPQLA 142
>At5g03415.1 68418.m00294 DPB-1 transcription factor, putative (DPB)
similar to Swiss-Prot:Q14186 transcription factor DP-1
[Homo sapiens]; contains Pfam profile PF02319:
Transcription factor E2F/dimerisation partner (TDP)
Length = 385
Score = 30.3 bits (65), Expect = 1.3
Identities = 15/64 (23%), Positives = 37/64 (57%), Gaps = 3/64 (4%)
Frame = +2
Query: 119 SKGVQYLNEIKDSVVAGFQWAAKEGVMAEE---NLRGVRFNIYDVTLHMMPSIEVVAKSF 289
SKG NE+ D +VA F +G ++ + + +R +YD L+++ ++++++K
Sbjct: 117 SKGRTTYNEVADELVAEFALPNNDGTSPDQQQYDEKNIRRRVYD-ALNVLMAMDIISKDK 175
Query: 290 QQLE 301
++++
Sbjct: 176 KEIQ 179
>At5g61930.1 68418.m07774 expressed protein contains Pfam PF05634:
Arabidopsis thaliana protein of unknown function
(DUF794)
Length = 340
Score = 29.1 bits (62), Expect = 3.1
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = -3
Query: 630 PVQRCRACSEVRTGLQG 580
PV RCR C+EV G QG
Sbjct: 62 PVHRCRLCAEVHIGKQG 78
>At2g35490.1 68415.m04347 plastid-lipid associated protein PAP,
putative similar to plastid-lipid associated protein
PAP3 [Brassica rapa] GI:14248552; contains Pfam profile
PF04755: PAP_fibrillin
Length = 376
Score = 29.1 bits (62), Expect = 3.1
Identities = 20/61 (32%), Positives = 26/61 (42%), Gaps = 1/61 (1%)
Frame = +3
Query: 501 VRFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEPQSKPYNV-VQETRKRKGLKEGLPDLT 677
+R + S + V D W PGD EP S+P NV V G K G P+L
Sbjct: 52 IRSSLPSESESESDLDASAVTDEWGEKPGDANEPDSQPDNVTVNVITDEWGEKSG-PELE 110
Query: 678 Q 680
+
Sbjct: 111 E 111
>At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein weak
similarity to polygalacturonase PG1 [Glycine max]
GI:5669846; contains PF00295: Glycosyl hydrolases family
28
Length = 444
Score = 27.5 bits (58), Expect = 9.5
Identities = 11/22 (50%), Positives = 17/22 (77%), Gaps = 1/22 (4%)
Frame = +3
Query: 420 SWSRFRRVPGGRY-TYVHCEGL 482
S+ R R +PGGRY +++H +GL
Sbjct: 123 SYGRGRELPGGRYMSFIHGDGL 144
>At4g14990.1 68417.m02303 expressed protein
Length = 787
Score = 27.5 bits (58), Expect = 9.5
Identities = 17/58 (29%), Positives = 26/58 (44%)
Frame = -1
Query: 413 FSTP*IPPTATSGH*ISQRYTGSIRRG*AVSRHAYKHLLVVGMIWPPPLWMASYGVLH 240
FS P P + S +S G G ++R+A + M+ PP W+ G+LH
Sbjct: 214 FSAPNASPLSNSTFHLSGLSHGPSHYGNNLARYASCGPTLGNMVQQPPHWVTDPGLLH 271
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,727,477
Number of Sequences: 28952
Number of extensions: 418900
Number of successful extensions: 1203
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 1139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1197
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1565336320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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