Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0615
         (725 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po...   141   8e-35
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po...   132   6e-32
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb...    27   2.7  
SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce...    27   3.6  
SPBC6B1.04 |mde4||monopolin-like complex subunit Mde4|Schizosacc...    25   8.3  

>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 270

 Score =  141 bits (342), Expect = 8e-35
 Identities = 64/76 (84%), Positives = 73/76 (96%)
 Frame = +2

Query: 257 AKMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLI 436
           A++ PTHPIRLGLALNFSVFYYEILNSPD+AC LAKQAFD+AI+ELD+L+E+SYKDSTLI
Sbjct: 163 AELAPTHPIRLGLALNFSVFYYEILNSPDRACYLAKQAFDEAISELDSLSEESYKDSTLI 222

Query: 437 MQLLRDNLTLWTSDTQ 484
           MQLLRDNLTLWTSD +
Sbjct: 223 MQLLRDNLTLWTSDAE 238



 Score =  104 bits (249), Expect = 1e-23
 Identities = 48/89 (53%), Positives = 63/89 (70%)
 Frame = +3

Query: 15  QMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGE 194
           ++ KEYR K+E+EL  IC D+L +L+KHLIP A++ ESKVFY KMKGDYYRYLAE A GE
Sbjct: 82  ELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESKVFYYKMKGDYYRYLAEFAVGE 141

Query: 195 TRHSVVEDSQKAYQDAFEISRRKCSPHTP 281
            R    + S + Y+ A EI+  + +P  P
Sbjct: 142 KRQHSADQSLEGYKAASEIATAELAPTHP 170


>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 270

 Score =  132 bits (318), Expect = 6e-32
 Identities = 61/85 (71%), Positives = 73/85 (85%)
 Frame = +2

Query: 257 AKMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLI 436
           A++ PT P+RLGLALNFSVFYYEIL+SP+ AC LAKQ FD+AI+ELD+L+E+SYKDSTLI
Sbjct: 162 AELPPTDPMRLGLALNFSVFYYEILDSPESACHLAKQVFDEAISELDSLSEESYKDSTLI 221

Query: 437 MQLLRDNLTLWTSDTQGDGDEPAEA 511
           MQLLRDNLTLWTSD + +     EA
Sbjct: 222 MQLLRDNLTLWTSDAEYNQSAKEEA 246



 Score =  102 bits (245), Expect = 5e-23
 Identities = 48/92 (52%), Positives = 62/92 (67%)
 Frame = +3

Query: 6   RKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVA 185
           R+  + KEYR K+E EL +IC+DVL +L+KHLIP A+  ESKVFY KMKGDYYRYLAE  
Sbjct: 78  RQAALIKEYRKKIEDELSDICHDVLSVLEKHLIPAATTGESKVFYYKMKGDYYRYLAEFT 137

Query: 186 TGETRHSVVEDSQKAYQDAFEISRRKCSPHTP 281
            GE      + S +AY+ A +I+  +  P  P
Sbjct: 138 VGEVCKEAADSSLEAYKAASDIAVAELPPTDP 169


>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1778

 Score = 27.1 bits (57), Expect = 2.7
 Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 3/49 (6%)
 Frame = +3

Query: 84   LLDKHLIPKASNPESKVFYLKMKGDYYR---YLAEVATGETRHSVVEDS 221
            +++K  IP++   E+K  Y +  GD+     +L E A  E  H V+ D+
Sbjct: 1608 MIEKLCIPESWLNEAKALYARYVGDHLNELYFLQEAALYEDAHKVLLDT 1656


>SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 817

 Score = 26.6 bits (56), Expect = 3.6
 Identities = 16/70 (22%), Positives = 34/70 (48%)
 Frame = +2

Query: 317 YYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDTQGDGD 496
           Y ++    D   QLA Q F     +++ L  +++  S  ++Q ++DN  +  S T+   +
Sbjct: 449 YDDLFPYMDNFIQLAVQLFYHISKKVNCLYVEAFSSSDAVLQRIQDNAVV-DSPTEELSE 507

Query: 497 EPAEAATTNI 526
           + A    +N+
Sbjct: 508 QAARNRKSNV 517


>SPBC6B1.04 |mde4||monopolin-like complex subunit
           Mde4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 421

 Score = 25.4 bits (53), Expect = 8.3
 Identities = 16/43 (37%), Positives = 25/43 (58%), Gaps = 2/43 (4%)
 Frame = +3

Query: 3   ERKQQMAKEYRVKVEKELREICYDV--LGLLDKHLIPKASNPE 125
           E++Q  A +YR+KVE+   +I   V  +  L+  L  + SNPE
Sbjct: 85  EQEQNEANDYRLKVERLEHKISDYVQEINSLNSQLQIQKSNPE 127


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,836,616
Number of Sequences: 5004
Number of extensions: 56728
Number of successful extensions: 190
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 184
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 190
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 341222980
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)