Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0615
(725 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.) 131 6e-31
SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0) 129 3e-30
SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.) 75 4e-14
SB_37955| Best HMM Match : 14-3-3 (HMM E-Value=6.5861e-44) 49 3e-06
SB_27280| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.8
SB_23213| Best HMM Match : SH3_1 (HMM E-Value=9.2e-12) 29 3.8
SB_9205| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 5.1
SB_12646| Best HMM Match : SSrecog (HMM E-Value=0) 28 6.7
SB_3346| Best HMM Match : Spectrin (HMM E-Value=0.012) 28 8.9
>SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 309
Score = 131 bits (316), Expect = 6e-31
Identities = 62/81 (76%), Positives = 72/81 (88%), Gaps = 1/81 (1%)
Frame = +2
Query: 260 KMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLIM 439
K+ PT PIRLGLALNFSVF+YEI + ++AC+LAKQAFDDAIAELD+LNED YKDSTLIM
Sbjct: 229 KLAPTDPIRLGLALNFSVFHYEIQENQEEACKLAKQAFDDAIAELDSLNEDQYKDSTLIM 288
Query: 440 QLLRDNLTLWTSDTQGD-GDE 499
QLLRDNLTLW+S+ Q D GD+
Sbjct: 289 QLLRDNLTLWSSENQEDQGDD 309
Score = 121 bits (292), Expect = 5e-28
Identities = 61/96 (63%), Positives = 70/96 (72%), Gaps = 2/96 (2%)
Frame = +2
Query: 260 KMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLIM 439
K+ PT PIRLGLALNFSVFYYEI+ +AC LAK+AFDDAIAELDTL+ED YKDSTLIM
Sbjct: 140 KLPPTDPIRLGLALNFSVFYYEIVEDSKQACDLAKKAFDDAIAELDTLSEDQYKDSTLIM 199
Query: 440 QLLRDNLTLWTSDTQG--DGDEPAEAATTNISPHSP 541
QLLRDNLT+ Q + E AE ++P P
Sbjct: 200 QLLRDNLTVVEKALQAYKEAKEAAETGDGKLAPTDP 235
Score = 99.5 bits (237), Expect = 2e-21
Identities = 50/95 (52%), Positives = 62/95 (65%), Gaps = 2/95 (2%)
Frame = +3
Query: 3 ERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEV 182
ERK+Q + YR +E EL E+C VL LL+ LIP A + ESKVFYLKMKGDYYRY EV
Sbjct: 52 ERKKQNTETYRQTIENELNEVCETVLKLLESKLIPNAQSTESKVFYLKMKGDYYRYEGEV 111
Query: 183 ATGETRHSVVEDSQKAYQDAFEISRR--KCSPHTP 281
A + R VV+ + KAY +A EI+ + K P P
Sbjct: 112 AGADRRREVVQKAMKAYSEAQEIAEKDPKLPPTDP 146
>SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0)
Length = 248
Score = 129 bits (311), Expect = 3e-30
Identities = 58/80 (72%), Positives = 69/80 (86%)
Frame = +2
Query: 260 KMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLIM 439
++ P+HPIRLGLALNFSVFYYEI N P +AC+LAK+AFDDAIA LD L ++SYKDSTLIM
Sbjct: 167 ELSPSHPIRLGLALNFSVFYYEIENKPPEACKLAKEAFDDAIAVLDNLKDESYKDSTLIM 226
Query: 440 QLLRDNLTLWTSDTQGDGDE 499
QLLRDNLTLWTS+ +G +
Sbjct: 227 QLLRDNLTLWTSEQDQEGQD 246
Score = 78.6 bits (185), Expect = 5e-15
Identities = 40/96 (41%), Positives = 58/96 (60%), Gaps = 3/96 (3%)
Frame = +3
Query: 3 ERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKAS---NPESKVFYLKMKGDYYRYL 173
E + K+YR + EL C +VL +L+ +L+ N E+KVFYLKM+GDY+RYL
Sbjct: 79 EEMAALTKKYREDITNELNGKCAEVLDILENYLLKDGQDDINTEAKVFYLKMRGDYHRYL 138
Query: 174 AEVATGETRHSVVEDSQKAYQDAFEISRRKCSPHTP 281
EVA G++R +E S++AY+DA + SP P
Sbjct: 139 VEVAEGDSRKENIEKSREAYKDA-SAKAEELSPSHP 173
>SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 219
Score = 75.4 bits (177), Expect = 4e-14
Identities = 32/59 (54%), Positives = 48/59 (81%)
Frame = +3
Query: 18 MAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGE 194
+ K+Y+ +EKEL+++C +VLG+L++ LIP A + E+KVFY K+KGDYYRYLAE + G+
Sbjct: 158 IVKKYKACIEKELKDLCKEVLGILER-LIPGAEDEENKVFYFKLKGDYYRYLAEFSHGQ 215
>SB_37955| Best HMM Match : 14-3-3 (HMM E-Value=6.5861e-44)
Length = 251
Score = 49.2 bits (112), Expect = 3e-06
Identities = 22/47 (46%), Positives = 32/47 (68%)
Frame = +3
Query: 9 KQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKM 149
K +M + YR +E+EL+ IC ++L LLD LI + + ESKVFY K+
Sbjct: 79 KLEMIRNYRKTIEEELKTICGEILSLLDDSLIKNSQSEESKVFYNKI 125
>SB_27280| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 980
Score = 29.1 bits (62), Expect = 3.8
Identities = 11/27 (40%), Positives = 14/27 (51%)
Frame = +2
Query: 476 DTQGDGDEPAEAATTNISPHSPQHTHL 556
D +G P A T PH P+HTH+
Sbjct: 178 DIRGTNTIPVHATTHKTLPHIPRHTHI 204
>SB_23213| Best HMM Match : SH3_1 (HMM E-Value=9.2e-12)
Length = 979
Score = 29.1 bits (62), Expect = 3.8
Identities = 17/47 (36%), Positives = 24/47 (51%), Gaps = 3/47 (6%)
Frame = +2
Query: 374 DDAIAELDTLNEDSYKDSTLIMQL---LRDNLTLWTSDTQGDGDEPA 505
DD + D ++DS + + QL L+ + LW S TQGD D A
Sbjct: 850 DDDDFDTDEWSDDSDAEGSAAGQLKLCLKREILLWKSGTQGDADRRA 896
>SB_9205| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 54
Score = 28.7 bits (61), Expect = 5.1
Identities = 14/47 (29%), Positives = 24/47 (51%)
Frame = +2
Query: 236 RCF*NQQAKMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFD 376
+C +QAK++ PI G+ L+ + + E+L K + A FD
Sbjct: 2 KCVTPEQAKIEDDRPITPGIGLSSAATFEELLEKQLKLEEKANMTFD 48
>SB_12646| Best HMM Match : SSrecog (HMM E-Value=0)
Length = 783
Score = 28.3 bits (60), Expect = 6.7
Identities = 11/34 (32%), Positives = 22/34 (64%)
Frame = +3
Query: 9 KQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 110
K++M ++Y K+EKE+ Y+++ L K ++ K
Sbjct: 292 KEEMKEKYDGKIEKEMSGAIYEIISRLMKAVVGK 325
>SB_3346| Best HMM Match : Spectrin (HMM E-Value=0.012)
Length = 983
Score = 27.9 bits (59), Expect = 8.9
Identities = 17/58 (29%), Positives = 27/58 (46%)
Frame = +2
Query: 317 YYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDTQGD 490
YY I + K Q FDD + +ED+ +D+T ++L+ T+D GD
Sbjct: 505 YYNIPYNKPKKNDFKLQLFDDYKQAVTQASEDTLRDATTAIELMNAP----TTDVHGD 558
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,613,968
Number of Sequences: 59808
Number of extensions: 441044
Number of successful extensions: 1268
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 1145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1266
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1937927537
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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