Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0614
         (575 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC4C3.07 |||translation initiation factor eIF3f|Schizosaccharo...    73   4e-14
SPCC1682.10 |rpn8||19S proteasome regulatory subunit Rpn8|Schizo...    45   7e-06
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual    27   1.5  
SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb...    27   2.6  
SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosacch...    27   2.6  
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce...    26   3.4  
SPBC725.05c |||nucleotide pyrophosphatase |Schizosaccharomyces p...    26   3.4  
SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc...    26   4.5  
SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr...    25   6.0  
SPBC19F5.03 |||inositol polyphosphate phosphatase |Schizosacchar...    25   7.9  

>SPBC4C3.07 |||translation initiation factor
           eIF3f|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 302

 Score = 72.5 bits (170), Expect = 4e-14
 Identities = 33/83 (39%), Positives = 55/83 (66%), Gaps = 2/83 (2%)
 Frame = +1

Query: 40  ISVKVHPVVLFQIVDAYERRNADSHRVIGTLLGT--SDKGVVEVTNCFCVPHKEHADQVE 213
           +++ + P VLF I+D   R++ ++ RVIGTLLGT   D   +E+ +CF VPH E ++QVE
Sbjct: 21  LNIVIEPAVLFSILDHSTRKSENNQRVIGTLLGTRSEDGREIEIKSCFAVPHNESSEQVE 80

Query: 214 AELNYAMDVYELNRESIPLKVLL 282
            E+ Y   +Y L+ ++ P +V++
Sbjct: 81  VEMEYHRAMYHLHLKANPREVVV 103



 Score = 36.3 bits (80), Expect = 0.003
 Identities = 24/91 (26%), Positives = 43/91 (47%), Gaps = 13/91 (14%)
 Frame = +3

Query: 255 RVNSSESIVGWWATGNEVTNHSSVIHEYYSRECREP------------VHVTLDTSLAGG 398
           + N  E +VGW+AT  ++   S++I   Y+    EP            VH+T++T ++  
Sbjct: 95  KANPREVVVGWYATSPDLDAFSALIQNLYASPA-EPGTAPLGTYPHPCVHLTVNTDVS-S 152

Query: 399 RMGLRAYVCVPLGVPNG-KQGCMFTPVDVTL 488
            + ++ YV  P+G+       C F P   T+
Sbjct: 153 PLAIKTYVSSPVGITERLADSCAFVPTPFTI 183


>SPCC1682.10 |rpn8||19S proteasome regulatory subunit
           Rpn8|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 324

 Score = 45.2 bits (102), Expect = 7e-06
 Identities = 22/51 (43%), Positives = 30/51 (58%), Gaps = 1/51 (1%)
 Frame = +1

Query: 46  VKVHPVVLFQIVDAYERR-NADSHRVIGTLLGTSDKGVVEVTNCFCVPHKE 195
           V VHP+VL   VD+Y R       RV+G LLG ++  VV V N + +P +E
Sbjct: 17  VIVHPLVLLSAVDSYNRSAKGTKRRVVGILLGQNNGDVVNVANSYAIPFEE 67


>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1611

 Score = 27.5 bits (58), Expect = 1.5
 Identities = 21/63 (33%), Positives = 34/63 (53%)
 Frame = -2

Query: 571  GHAARPATSTRHSLLAHL*TYISGS*HVRVTSTGVNMQPCFPFGTPNGTQTYARKPIRPP 392
            GHA   ++STR SL AH  +  S   H+  +S+  + +P     T    ++++ KP+ P 
Sbjct: 1314 GHA--DSSSTRTSL-AHQDSRKSLHRHLSRSSSRASKKPSIVSTTGPFNESFSAKPVEPC 1370

Query: 391  ASE 383
            ASE
Sbjct: 1371 ASE 1373


>SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1334

 Score = 26.6 bits (56), Expect = 2.6
 Identities = 13/42 (30%), Positives = 21/42 (50%)
 Frame = +1

Query: 88  YERRNADSHRVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVE 213
           Y+ R  DSHR I  L GT  +  +   +   + +K H  ++E
Sbjct: 483 YDHRLRDSHREIYQLQGTGYRPFLRANDNASINNKNHHKELE 524


>SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1318

 Score = 26.6 bits (56), Expect = 2.6
 Identities = 17/60 (28%), Positives = 33/60 (55%), Gaps = 1/60 (1%)
 Frame = -1

Query: 515 DLH-LRFIACESDIYRSEHATLLSIWHSQWYTNICT*THSTSSQRSVQSNMDRLTAFTGI 339
           D+H L+F+  +  +  S  A +L  +   W+ N+      T S+RS+ +N D +T+F+ +
Sbjct: 396 DIHALKFL--KRWVRASAKARILDSFCIDWHLNV-----QTMSERSILTNRDFITSFSAV 448


>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 2100

 Score = 26.2 bits (55), Expect = 3.4
 Identities = 11/29 (37%), Positives = 14/29 (48%)
 Frame = +3

Query: 24   KNGSQY*CEGPSCCFISNCGRIRTSKCRF 110
            K   Q+  + P C  IS   R   SKCR+
Sbjct: 1951 KQAPQFQSKSPECVIISTMNRSCDSKCRY 1979


>SPBC725.05c |||nucleotide pyrophosphatase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 485

 Score = 26.2 bits (55), Expect = 3.4
 Identities = 16/62 (25%), Positives = 31/62 (50%), Gaps = 5/62 (8%)
 Frame = +1

Query: 34  LNISVKVHPVVLFQIVDAYERRNADSHRVI-----GTLLGTSDKGVVEVTNCFCVPHKEH 198
           LNI ++   +V+ ++++  ++RN D H  I       +  TSD  ++ + N F +    H
Sbjct: 253 LNIIIQEVDIVIGELIEGLKKRNIDKHVNIIFLSDHGMAPTSDNRLIWLDNMFNLSAVAH 312

Query: 199 AD 204
            D
Sbjct: 313 RD 314


>SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase
           Cdc25|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 596

 Score = 25.8 bits (54), Expect = 4.5
 Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 1/49 (2%)
 Frame = +3

Query: 99  KCRFSQSYRHLIGHERQRSGG-SNQLLLRATQRTCRSSRSGT*LRDGCL 242
           + R S SY   I  ++  SGG + + L  A  RTC  S + T L + CL
Sbjct: 201 RSRSSSSYS--INKKKGTSGGQATRHLTYALSRTCSQSSNTTSLLESCL 247


>SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 131

 Score = 25.4 bits (53), Expect = 6.0
 Identities = 6/11 (54%), Positives = 10/11 (90%)
 Frame = +2

Query: 461 HVHSCRCHSHM 493
           H+H CRCH+++
Sbjct: 69  HIHCCRCHTYI 79


>SPBC19F5.03 |||inositol polyphosphate phosphatase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 598

 Score = 25.0 bits (52), Expect = 7.9
 Identities = 12/41 (29%), Positives = 19/41 (46%)
 Frame = +3

Query: 186 TQRTCRSSRSGT*LRDGCLRAEQRVNSSESIVGWWATGNEV 308
           TQ+    SR    +R  C+    R N  +S +G W   N++
Sbjct: 362 TQKYRVLSRQNGVVRSNCMDCLDRTNVVQSCIGRWVLTNQL 402


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,691,553
Number of Sequences: 5004
Number of extensions: 59232
Number of successful extensions: 182
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 172
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 180
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 246098644
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)