BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0591 (688 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC736.07c |||cell polarity protein |Schizosaccharomyces pombe|... 28 1.5 SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase Abc... 27 2.5 SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomy... 27 2.5 SPAC2F7.16c |||phospholipase D |Schizosaccharomyces pombe|chr 1|... 27 2.5 SPBC3B8.04c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 5.9 >SPCC736.07c |||cell polarity protein |Schizosaccharomyces pombe|chr 3|||Manual Length = 699 Score = 27.9 bits (59), Expect = 1.5 Identities = 18/49 (36%), Positives = 26/49 (53%) Frame = +1 Query: 61 GESDPRNPTASSC*KTRCGESQRGKEAKSETPKAGQGGGSR*S*KVQTG 207 GES+ N T++S K R + K+AK ET K+G + S K+ G Sbjct: 297 GESNKDNNTSTSKHKKRPKRLSKFKQAKLETKKSGNKDHATSSEKLSLG 345 >SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase Abc2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1463 Score = 27.1 bits (57), Expect = 2.5 Identities = 17/47 (36%), Positives = 27/47 (57%), Gaps = 1/47 (2%) Frame = -2 Query: 402 AVVDVEFGFDVIHQIQDVFDDRLLLCLDHFVH-LFDLNFGLGIDLGR 265 A VDVE V I++ F+DR +L + H ++ + D N L +D G+ Sbjct: 1389 AAVDVETDAIVQRTIRERFNDRTILTIAHRINTVMDSNRILVLDHGK 1435 >SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1010 Score = 27.1 bits (57), Expect = 2.5 Identities = 16/41 (39%), Positives = 23/41 (56%), Gaps = 2/41 (4%) Frame = -3 Query: 593 HFDISFSAPCCVFF*--LSRLQENLPTYACSMICQINIQFF 477 HFDI+ A C + F LSRL E CS I ++ +++F Sbjct: 376 HFDITSDA-CMLNFSHVLSRLSEPFLDIGCSKIDRVQVEYF 415 >SPAC2F7.16c |||phospholipase D |Schizosaccharomyces pombe|chr 1|||Manual Length = 1369 Score = 27.1 bits (57), Expect = 2.5 Identities = 13/38 (34%), Positives = 20/38 (52%) Frame = +1 Query: 64 ESDPRNPTASSC*KTRCGESQRGKEAKSETPKAGQGGG 177 E+DP+NP A S G + +++K+E PK G Sbjct: 1284 ENDPKNPKAGS---QGSGNTSASEDSKTEKPKTRTNNG 1318 >SPBC3B8.04c |||membrane transporter|Schizosaccharomyces pombe|chr 2|||Manual Length = 867 Score = 25.8 bits (54), Expect = 5.9 Identities = 14/40 (35%), Positives = 21/40 (52%) Frame = -3 Query: 386 SSGLMSYTRFKTSLMTASFCVWTILFISSILTLVSASILA 267 SSGL+ K +S + +L I S LTLV +S ++ Sbjct: 732 SSGLLELIALKIGNAVSSLNTFRVLLIFSALTLVVSSFIS 771 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,426,572 Number of Sequences: 5004 Number of extensions: 42684 Number of successful extensions: 115 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 114 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 114 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 317927284 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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