BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0586
(613 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY217747-1|AAP45005.1| 246|Apis mellifera short-chain dehydroge... 30 0.021
DQ026034-1|AAY87893.1| 569|Apis mellifera nicotinic acetylcholi... 25 0.77
DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholi... 25 0.77
DQ342041-1|ABC69933.1| 828|Apis mellifera STIP protein. 23 3.1
AY500239-1|AAR92109.1| 555|Apis mellifera neuronal nicotinic ac... 21 7.2
>AY217747-1|AAP45005.1| 246|Apis mellifera short-chain
dehydrogenase/reductase protein.
Length = 246
Score = 29.9 bits (64), Expect = 0.021
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = +2
Query: 77 VGRLALVTGAGSGIGRAACQVLSKEGATVI 166
+ +ALVTGA SGIG+ + L +G VI
Sbjct: 6 IDEVALVTGANSGIGKCLIECLVGKGMKVI 35
>DQ026034-1|AAY87893.1| 569|Apis mellifera nicotinic acetylcholine
receptor alpha4subunit protein.
Length = 569
Score = 24.6 bits (51), Expect = 0.77
Identities = 19/65 (29%), Positives = 31/65 (47%)
Frame = +1
Query: 250 AYAVELDVSNSKAVQDLVQDTLKQYKKPPCIIVNSAGITRDNWMLKLSEEDYDSVLNVNL 429
A V V+ + + L D L Y K +VN++ + R LKLS+ +++VNL
Sbjct: 16 ALVVHGAVAGNPDAKRLYDDLLSNYNKLVRPVVNTSDVLRVCIKLKLSQ-----LIDVNL 70
Query: 430 KGTFL 444
K +
Sbjct: 71 KNQIM 75
>DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholine
receptor alpha4subunit protein.
Length = 569
Score = 24.6 bits (51), Expect = 0.77
Identities = 19/65 (29%), Positives = 31/65 (47%)
Frame = +1
Query: 250 AYAVELDVSNSKAVQDLVQDTLKQYKKPPCIIVNSAGITRDNWMLKLSEEDYDSVLNVNL 429
A V V+ + + L D L Y K +VN++ + R LKLS+ +++VNL
Sbjct: 16 ALVVHGAVAGNPDAKRLYDDLLSNYNKLVRPVVNTSDVLRVCIKLKLSQ-----LIDVNL 70
Query: 430 KGTFL 444
K +
Sbjct: 71 KNQIM 75
>DQ342041-1|ABC69933.1| 828|Apis mellifera STIP protein.
Length = 828
Score = 22.6 bits (46), Expect = 3.1
Identities = 7/13 (53%), Positives = 10/13 (76%)
Frame = -1
Query: 286 LYCWKRLIPQHML 248
+Y WK LIP H++
Sbjct: 630 VYEWKELIPSHIM 642
>AY500239-1|AAR92109.1| 555|Apis mellifera neuronal nicotinic
acetylcholine receptoralpha7-1 protein.
Length = 555
Score = 21.4 bits (43), Expect = 7.2
Identities = 10/34 (29%), Positives = 18/34 (52%)
Frame = -2
Query: 183 YFLSAAITVAPSFDKTWHAARPIPEPAPVTRARR 82
Y+L + ++ DK A+ +P+PVT A +
Sbjct: 338 YWLPCILRMSRPSDKEEREAQKSQKPSPVTGASK 371
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 182,315
Number of Sequences: 438
Number of extensions: 4095
Number of successful extensions: 7
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7
length of database: 146,343
effective HSP length: 55
effective length of database: 122,253
effective search space used: 18093444
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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