BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0583 (648 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g20290.1 68418.m02415 40S ribosomal protein S8 (RPS8A) riboso... 106 1e-23 At5g59240.1 68418.m07424 40S ribosomal protein S8 (RPS8B) 40S ri... 105 3e-23 At2g22830.1 68415.m02711 squalene monooxygenase, putative / squa... 30 1.5 At1g73260.1 68414.m08478 trypsin and protease inhibitor family p... 29 2.7 At4g37760.1 68417.m05345 squalene monooxygenase, putative / squa... 28 6.1 At3g28360.1 68416.m03544 ABC transporter family protein similar ... 27 8.1 >At5g20290.1 68418.m02415 40S ribosomal protein S8 (RPS8A) ribosomal protein S8 - Zea mays, PIR:T04088 Length = 222 Score = 106 bits (254), Expect = 1e-23 Identities = 48/73 (65%), Positives = 59/73 (80%), Gaps = 1/73 (1%) Frame = +1 Query: 37 GKRAPIRKKRKYELGRPAANTRLGPQR-IHSVRSRGGNTKYRALRLDTGNFSWGSECSTR 213 GK+ RKKRKYE+GR ANT+L + + +R RGGN K+RALRLDTGN+SWGSE +TR Sbjct: 16 GKQKQWRKKRKYEMGRQPANTKLSSNKTVRRIRVRGGNVKWRALRLDTGNYSWGSEATTR 75 Query: 214 KTRIIDVVYNASN 252 KTR++DVVYNASN Sbjct: 76 KTRVLDVVYNASN 88 Score = 81.4 bits (192), Expect = 5e-16 Identities = 49/95 (51%), Positives = 60/95 (63%), Gaps = 10/95 (10%) Frame = +3 Query: 255 ELVRTKTLVKNAIVVVDATPFRQWYESHYTLPLGR-KKGAKLT-----EAEEAII----N 404 ELVRTKTLVK+AIV VDA PF+QWY SHY + LGR KK A T E EEA + Sbjct: 90 ELVRTKTLVKSAIVQVDAAPFKQWYLSHYGVELGRKKKSASSTKKDGEEGEEAAVAAPEE 149 Query: 405 KKRSQKTARKYLARQRLAKVEGALEEQFHTGRLLA 509 K+S RK +RQ ++ +E+QF +GRLLA Sbjct: 150 VKKSNHLLRKIASRQEGRSLDSHIEDQFASGRLLA 184 Score = 68.5 bits (160), Expect = 4e-12 Identities = 27/35 (77%), Positives = 33/35 (94%) Frame = +2 Query: 509 CVASRPGQCGRADGYILEGKELEFYLRKIKSKRAK 613 C++SRPGQCGRADGYILEGKELEFY++KI+ K+ K Sbjct: 185 CISSRPGQCGRADGYILEGKELEFYMKKIQKKKGK 219 >At5g59240.1 68418.m07424 40S ribosomal protein S8 (RPS8B) 40S ribosomal protein S8, Prunus armeniaca, EMBL:AF071889 Length = 210 Score = 105 bits (252), Expect = 3e-23 Identities = 50/73 (68%), Positives = 57/73 (78%), Gaps = 1/73 (1%) Frame = +1 Query: 37 GKRAPIRKKRKYELGRPAANTRLGPQR-IHSVRSRGGNTKYRALRLDTGNFSWGSECSTR 213 GK+ RKKRKYELGR ANT+L + + +R RGGN K+RALRLDTGNFSWGSE TR Sbjct: 16 GKQKMWRKKRKYELGRQPANTKLSSNKTVRRIRVRGGNVKWRALRLDTGNFSWGSEAVTR 75 Query: 214 KTRIIDVVYNASN 252 KTRI+DV YNASN Sbjct: 76 KTRILDVAYNASN 88 Score = 77.4 bits (182), Expect = 8e-15 Identities = 43/85 (50%), Positives = 53/85 (62%) Frame = +3 Query: 255 ELVRTKTLVKNAIVVVDATPFRQWYESHYTLPLGRKKGAKLTEAEEAIINKKRSQKTARK 434 ELVRT+TLVK+AIV VDA PF+Q Y HY + +GRKK + EE K+S RK Sbjct: 90 ELVRTQTLVKSAIVQVDAAPFKQGYLQHYGVDIGRKKKGEAVTTEEV----KKSNHVQRK 145 Query: 435 YLARQRLAKVEGALEEQFHTGRLLA 509 RQ ++ LEEQF +GRLLA Sbjct: 146 LEMRQEGRALDSHLEEQFSSGRLLA 170 Score = 68.9 bits (161), Expect = 3e-12 Identities = 27/35 (77%), Positives = 33/35 (94%) Frame = +2 Query: 509 CVASRPGQCGRADGYILEGKELEFYLRKIKSKRAK 613 C+ASRPGQCGRADGYILEGKELEFY++K++ K+ K Sbjct: 171 CIASRPGQCGRADGYILEGKELEFYMKKLQKKKGK 205 >At2g22830.1 68415.m02711 squalene monooxygenase, putative / squalene epoxidase, putative similar to SP|O65404 (SE 1,1), SP|O65402 (SE 1,2), SP|O65403 (SE 2) Length = 585 Score = 29.9 bits (64), Expect = 1.5 Identities = 16/49 (32%), Positives = 23/49 (46%) Frame = -3 Query: 337 CDSYHCLNGVASTTTIAFLTRVFVRTNSC*MHYTQHQ*YGFCELNIPIP 191 C Y L GV S+ +A L+ + R S +H+ Y C L +P P Sbjct: 492 CFDYLSLGGVFSSGPVALLSGLNPRPLSLVLHFFAVAIYAVCRLMLPFP 540 >At1g73260.1 68414.m08478 trypsin and protease inhibitor family protein / Kunitz family protein similar to trypsin inhibitor propeptide [Brassica oleracea] GI:841208; contains Pfam profile PF00197: Trypsin and protease inhibitor Length = 215 Score = 29.1 bits (62), Expect = 2.7 Identities = 18/44 (40%), Positives = 23/44 (52%), Gaps = 1/44 (2%) Frame = +3 Query: 258 LVRTKTLVKNAI-VVVDATPFRQWYESHYTLPLGRKKGAKLTEA 386 L T L NA VVD ++ES+Y LP+ R +G LT A Sbjct: 16 LALTAVLASNAYGAVVDIDGNAMFHESYYVLPVIRGRGGGLTLA 59 >At4g37760.1 68417.m05345 squalene monooxygenase, putative / squalene epoxidase, putative similar to SP|O65404 (SE 1,1), SP|O65402 (SE 1,2), SP|O65403 (SE 2) Length = 525 Score = 27.9 bits (59), Expect = 6.1 Identities = 15/49 (30%), Positives = 24/49 (48%) Frame = -3 Query: 337 CDSYHCLNGVASTTTIAFLTRVFVRTNSC*MHYTQHQ*YGFCELNIPIP 191 C Y L GV S+ +A L+ + R S +H+ +G L +P+P Sbjct: 424 CFDYLSLGGVCSSGPVALLSGLNPRPMSLVLHFFAVAIFGVGRLLVPLP 472 >At3g28360.1 68416.m03544 ABC transporter family protein similar to P-glycoprotein homologue GI:2292907 from [Hordeum vulgare subsp. vulgare] Length = 1158 Score = 27.5 bits (58), Expect = 8.1 Identities = 12/51 (23%), Positives = 27/51 (52%) Frame = -2 Query: 308 CIYYNNCILDKGLCTHQFLLDALYTTSMIRVLRVEHSDPQEKLPVSRRSAR 156 C+ +N CI++ G +D Y TS++R+ ++++ + + V + R Sbjct: 483 CVLHNGCIVETGSHDKLMEIDGKY-TSLVRLQQMKNEESCDNTSVGVKEGR 532 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,453,679 Number of Sequences: 28952 Number of extensions: 267370 Number of successful extensions: 720 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 697 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 716 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1344285648 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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