Bombyx BLAST Result against bee

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0579
         (653 letters)

Database: bee 
           438 sequences; 146,343 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF469010-1|AAL93136.1|  678|Apis mellifera cGMP-dependent protei...    74   1e-15
AY739658-1|AAU85297.1|  664|Apis mellifera hyperpolarization-act...    35   8e-04
AY280848-1|AAQ16312.1|  632|Apis mellifera hyperpolarization-act...    35   8e-04
DQ667195-1|ABG75747.1|  469|Apis mellifera cys-loop ligand-gated...    30   0.022
EF625897-1|ABR45904.1|  684|Apis mellifera hexamerin protein.          23   3.4  
EF591128-1|ABQ59246.1|  684|Apis mellifera hexamerin 70a protein.      23   3.4  
AB204559-1|BAD89804.1|  832|Apis mellifera soluble guanylyl cycl...    21   7.9  

>AF469010-1|AAL93136.1|  678|Apis mellifera cGMP-dependent protein
           kinase foraging protein.
          Length = 678

 Score = 73.7 bits (173), Expect = 1e-15
 Identities = 33/84 (39%), Positives = 51/84 (60%)
 Frame = +2

Query: 263 LFVSASGQFEALKSGQVVKNFGPGEVFGELAILYKAKRFASIRCITEARVWTLERRVFQK 442
           ++V   G+ E  + G+ +    PG+V GELAILY  KR A+I   T+ ++W ++R+ FQ 
Sbjct: 139 VYVMEEGKVEVSRDGKYLSTLAPGKVLGELAILYNCKRTATITAATDCQLWAIDRQCFQT 198

Query: 443 IMVRSERQEQEDNIRFLSSVPLCK 514
           IM+R+    Q +   FL SVP+ K
Sbjct: 199 IMMRTGLSRQAEYTDFLKSVPIFK 222



 Score = 46.0 bits (104), Expect = 3e-07
 Identities = 24/65 (36%), Positives = 38/65 (58%), Gaps = 3/65 (4%)
 Frame = +1

Query: 466 GTGRQYSFSFFCAALQEIHHIE---LAQISDFLKREFFSTGTAVVRQGDRGDKFYIIRGG 636
           G  RQ  ++ F  ++    ++    L +ISD L+  F++ G  ++RQG RGD F+II  G
Sbjct: 204 GLSRQAEYTDFLKSVPIFKNLPEETLIKISDVLEETFYNNGDYIIRQGARGDTFFIISRG 263

Query: 637 EVVVT 651
           +V VT
Sbjct: 264 QVRVT 268



 Score = 43.2 bits (97), Expect = 2e-06
 Identities = 29/82 (35%), Positives = 44/82 (53%)
 Frame = +3

Query: 99  KDERSAEQIRKAIMMNDFLRNLMDDERLNAVVGAMMTQDFEAGSLIIREGESEVICLFRL 278
           K +RS E I+ AI+ NDF++NL +  ++    G  ++  F AGS IIREG+   I     
Sbjct: 85  KRDRSRELIKAAILANDFMKNL-ELTQIRRDRGLHVSCSFSAGSTIIREGDVGSIVYVME 143

Query: 279 QDNLKR*KVAKSLRTLDQAKFL 344
           +  ++  +  K L TL   K L
Sbjct: 144 EGKVEVSRDGKYLSTLAPGKVL 165


>AY739658-1|AAU85297.1|  664|Apis mellifera
           hyperpolarization-activated ion channelvariant L
           protein.
          Length = 664

 Score = 34.7 bits (76), Expect = 8e-04
 Identities = 15/51 (29%), Positives = 28/51 (54%)
 Frame = +2

Query: 296 LKSGQVVKNFGPGEVFGELAILYKAKRFASIRCITEARVWTLERRVFQKIM 448
           + +G+V  +   G  FGE+ +L  A+R AS+R  T   +++L    F  ++
Sbjct: 505 MANGEVATSLSDGSYFGEICLLTNARRVASVRAETYCNLFSLSVDHFNAVL 555


>AY280848-1|AAQ16312.1|  632|Apis mellifera
           hyperpolarization-activated ion channel protein.
          Length = 632

 Score = 34.7 bits (76), Expect = 8e-04
 Identities = 15/51 (29%), Positives = 28/51 (54%)
 Frame = +2

Query: 296 LKSGQVVKNFGPGEVFGELAILYKAKRFASIRCITEARVWTLERRVFQKIM 448
           + +G+V  +   G  FGE+ +L  A+R AS+R  T   +++L    F  ++
Sbjct: 473 MANGEVATSLSDGSYFGEICLLTNARRVASVRAETYCNLFSLSVDHFNAVL 523


>DQ667195-1|ABG75747.1|  469|Apis mellifera cys-loop ligand-gated
           ion channel subunit protein.
          Length = 469

 Score = 29.9 bits (64), Expect = 0.022
 Identities = 19/43 (44%), Positives = 24/43 (55%)
 Frame = -1

Query: 638 SPPRIM*NLSPRSPCLTTAVPVEKNSLFKKSDICANSIWCISC 510
           SPPRI+   +     +T A  VE NSL K S I A+ IW + C
Sbjct: 260 SPPRIVLGTNTILTFMTLASKVE-NSLPKVSYIKASEIWFLGC 301


>EF625897-1|ABR45904.1|  684|Apis mellifera hexamerin protein.
          Length = 684

 Score = 22.6 bits (46), Expect = 3.4
 Identities = 10/26 (38%), Positives = 13/26 (50%)
 Frame = -1

Query: 416 STPSPRLYIVLKRIVWLYIRLPVHQK 339
           S   P  Y + KRI+  Y    +HQK
Sbjct: 412 SMKDPAFYRIYKRIIDYYHSYKMHQK 437


>EF591128-1|ABQ59246.1|  684|Apis mellifera hexamerin 70a protein.
          Length = 684

 Score = 22.6 bits (46), Expect = 3.4
 Identities = 10/26 (38%), Positives = 13/26 (50%)
 Frame = -1

Query: 416 STPSPRLYIVLKRIVWLYIRLPVHQK 339
           S   P  Y + KRI+  Y    +HQK
Sbjct: 412 SMKDPAFYRIYKRIIDYYHSYKMHQK 437


>AB204559-1|BAD89804.1|  832|Apis mellifera soluble guanylyl cyclase
           beta-3 protein.
          Length = 832

 Score = 21.4 bits (43), Expect = 7.9
 Identities = 12/47 (25%), Positives = 23/47 (48%)
 Frame = +3

Query: 192 VGAMMTQDFEAGSLIIREGESEVICLFRLQDNLKR*KVAKSLRTLDQ 332
           +  +   DF    ++    +S  + L   Q+ LK  K+ +S+R LD+
Sbjct: 340 INDLSMHDFSRDLMLAGTQQSVELKLALDQEQLKSKKLEESMRKLDE 386


  Database: bee
    Posted date:  Oct 23, 2007  1:17 PM
  Number of letters in database: 146,343
  Number of sequences in database:  438
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 185,999
Number of Sequences: 438
Number of extensions: 3750
Number of successful extensions: 11
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 10
length of database: 146,343
effective HSP length: 55
effective length of database: 122,253
effective search space used: 19804986
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)