BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0577 (605 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g72370.1 68414.m08371 40S ribosomal protein SA (RPSaA) identi... 117 5e-27 At3g04770.2 68416.m00514 40S ribosomal protein SA (RPSaB) identi... 112 2e-25 At3g04770.1 68416.m00513 40S ribosomal protein SA (RPSaB) identi... 111 5e-25 At5g23690.1 68418.m02777 polynucleotide adenylyltransferase fami... 31 0.78 At3g03600.1 68416.m00363 ribosomal protein S2, mitochondrial (RP... 31 0.78 At5g54855.1 68418.m06833 pollen Ole e 1 allergen and extensin fa... 29 3.2 At4g08550.1 68417.m01406 glutaredoxin-related contains Pfam prof... 28 4.2 At3g63180.1 68416.m07097 expressed protein 27 7.3 At3g54570.1 68416.m06038 calmodulin-binding protein-related cont... 27 7.3 At5g10470.1 68418.m01213 kinesin motor protein-related TH65 prot... 27 9.6 >At1g72370.1 68414.m08371 40S ribosomal protein SA (RPSaA) identical to laminin receptor-like protein GB:U01955 [Arabidopsis thaliana]; identical to cDNA laminin receptor homologue GI:16379 Length = 298 Score = 117 bits (282), Expect = 5e-27 Identities = 51/82 (62%), Positives = 63/82 (76%) Frame = +2 Query: 8 RLLIVLDPAQDHQPITEASYVNIPVIALCNTDSPLRFVDIAIPCNTKSSHSIGLMWWLLA 187 RLLI+ DP DHQPI E + NIP+IA C+TDSP+RFVDI IP N K HSIG ++WLLA Sbjct: 124 RLLILTDPRTDHQPIKEGALGNIPIIAFCDTDSPMRFVDIGIPANNKGKHSIGCLFWLLA 183 Query: 188 REVLRLRGVLPRDQRWDVVVDL 253 R VL++RG + Q+WDV+VDL Sbjct: 184 RMVLQMRGTIAAGQKWDVMVDL 205 >At3g04770.2 68416.m00514 40S ribosomal protein SA (RPSaB) identical to p40 protein homolog GB:AAB67866 [Arabidopsis thaliana]; similar to 40S ribosomal protein SA (P40) GB:O65751 [Cicer arietinum] Length = 280 Score = 112 bits (269), Expect = 2e-25 Identities = 50/82 (60%), Positives = 61/82 (74%) Frame = +2 Query: 8 RLLIVLDPAQDHQPITEASYVNIPVIALCNTDSPLRFVDIAIPCNTKSSHSIGLMWWLLA 187 RLLI+ DP DHQPI E + NIP IA C+TDSP+ FVDI IP N K HSIG ++WLLA Sbjct: 125 RLLILTDPRTDHQPIKEGALGNIPTIAFCDTDSPMGFVDIGIPANNKGKHSIGCLFWLLA 184 Query: 188 REVLRLRGVLPRDQRWDVVVDL 253 R VL++RG + Q+WDV+VDL Sbjct: 185 RMVLQMRGTILAAQKWDVMVDL 206 >At3g04770.1 68416.m00513 40S ribosomal protein SA (RPSaB) identical to p40 protein homolog GB:AAB67866 [Arabidopsis thaliana]; similar to 40S ribosomal protein SA (P40) GB:O65751 [Cicer arietinum] Length = 332 Score = 111 bits (266), Expect = 5e-25 Identities = 49/86 (56%), Positives = 63/86 (73%) Frame = +2 Query: 8 RLLIVLDPAQDHQPITEASYVNIPVIALCNTDSPLRFVDIAIPCNTKSSHSIGLMWWLLA 187 RLLI+ DP DHQPI E + NIP IA C+TDSP+ FVDI IP N K HSIG ++WLLA Sbjct: 125 RLLILTDPRTDHQPIKEGALGNIPTIAFCDTDSPMGFVDIGIPANNKGKHSIGCLFWLLA 184 Query: 188 REVLRLRGVLPRDQRWDVVVDLSSTV 265 R VL++RG + Q+WDV+V+ +T+ Sbjct: 185 RMVLQMRGTILAAQKWDVMVNSRNTI 210 >At5g23690.1 68418.m02777 polynucleotide adenylyltransferase family protein low similarity to SP|P13685 Poly(A) polymerase (EC 2.7.7.19) {Escherichia coli O157:H7}; contains Pfam profile PF01743: polyA polymerase family protein Length = 527 Score = 30.7 bits (66), Expect = 0.78 Identities = 16/38 (42%), Positives = 24/38 (63%) Frame = +2 Query: 284 RMNNKPRNRLWYQLNQK*LLLSMKTGMKHWSQWLHGLK 397 +++++PR+R W QLN K L LS K + L+GLK Sbjct: 54 KVSDEPRDREWKQLNSKDLGLSSSMIAKSTRKVLNGLK 91 >At3g03600.1 68416.m00363 ribosomal protein S2, mitochondrial (RPS2) identical to SP|Q9GCB9 Mitochondrial ribosomal protein S2 {Arabidopsis thaliana}; contains Pfam profile PF00318: ribosomal protein S2 Length = 219 Score = 30.7 bits (66), Expect = 0.78 Identities = 16/48 (33%), Positives = 26/48 (54%), Gaps = 1/48 (2%) Frame = +2 Query: 14 LIVLDPAQDHQPITEASYVNIPVIALCNTDSPLRFVD-IAIPCNTKSS 154 ++V D + I EAS + IPV+A+ + + PL F + I P + S Sbjct: 134 VVVFDSERKSSVILEASKLQIPVVAIVDPNVPLEFFEKITYPVPARDS 181 >At5g54855.1 68418.m06833 pollen Ole e 1 allergen and extensin family protein contains Pfam domain, PF01190: Pollen proteins Ole e I family Length = 146 Score = 28.7 bits (61), Expect = 3.2 Identities = 12/46 (26%), Positives = 22/46 (47%) Frame = +2 Query: 101 DSPLRFVDIAIPCNTKSSHSIGLMWWLLAREVLRLRGVLPRDQRWD 238 D L ++A+ C TKS + + ++ V + +P +RWD Sbjct: 49 DHVLEGAEVAVLCITKSGEVVNYQAFTNSKGVYTVAETMPESERWD 94 >At4g08550.1 68417.m01406 glutaredoxin-related contains Pfam profile PF04784: Protein of unknown function, DUF547, weak hit to PF00462: Glutaredoxin Length = 587 Score = 28.3 bits (60), Expect = 4.2 Identities = 13/49 (26%), Positives = 22/49 (44%), Gaps = 6/49 (12%) Frame = -3 Query: 603 LSCNIINQCRVFMQHSSLER------IHPTRALRWRRLCGGSCAPLILY 475 L C CR+F+ L I+PTR + ++ GG P++ + Sbjct: 232 LGCEECRGCRLFLHEKRLRYVEINIDIYPTRKVELEKISGGDVVPMVFF 280 >At3g63180.1 68416.m07097 expressed protein Length = 978 Score = 27.5 bits (58), Expect = 7.3 Identities = 20/65 (30%), Positives = 33/65 (50%), Gaps = 3/65 (4%) Frame = -2 Query: 532 EGAAVAAAVRR-QLCSTHLVPGQTILLEQGVLQKQELQRVQQAGRYLQ--PMKPLAPVFH 362 +GA ++A+ R + C+TH + I L Q L+ L + YL+ ++P A + Sbjct: 424 QGAPISASQPRPKRCATHFFIARNIQLHQHFLKTNHLPTPNKGSVYLKGGDLRPTAG--N 481 Query: 361 SSLHG 347 SLHG Sbjct: 482 PSLHG 486 >At3g54570.1 68416.m06038 calmodulin-binding protein-related contains similarity to potato calmodulin-binding protein PCBP GI:17933110 from [Solanum tuberosum] Length = 417 Score = 27.5 bits (58), Expect = 7.3 Identities = 9/16 (56%), Positives = 13/16 (81%) Frame = -2 Query: 160 RVGRLGVAWDSNVHKS 113 R GR+G WD+NVH++ Sbjct: 107 RSGRIGRCWDANVHRA 122 >At5g10470.1 68418.m01213 kinesin motor protein-related TH65 protein, Arabidopsis thaliana, EMBL:AJ001729; contains Pfam profile PF00225: Kinesin motor domain Length = 1273 Score = 27.1 bits (57), Expect = 9.6 Identities = 13/48 (27%), Positives = 24/48 (50%) Frame = -3 Query: 216 STPRSLSTSRANNHHIKPIEWEDLVLHGIAMSTNLSGESVLHKAITGM 73 +T RS STSR ++ P+ + D +HG ++ + L ++ M Sbjct: 829 NTGRSKSTSRGSSPGRSPVRYLDTQIHGFKVNIKAERRNKLASVVSRM 876 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,101,794 Number of Sequences: 28952 Number of extensions: 257267 Number of successful extensions: 706 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 692 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 706 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1206913392 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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