Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0575
(734 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g36190.1 68415.m04442 beta-fructosidase, putative / beta-fruc... 58 8e-09
At1g55120.1 68414.m06295 beta-fructosidase, putative / beta-fruc... 58 8e-09
At3g13790.1 68416.m01742 beta-fructosidase (BFRUCT1) / beta-fruc... 56 2e-08
At1g62660.1 68414.m07071 beta-fructosidase (BFRUCT3) / beta-fruc... 54 1e-07
At5g11920.1 68418.m01394 glycosyl hydrolase family 32 protein si... 54 1e-07
At3g52600.1 68416.m05794 beta-fructosidase, putative / beta-fruc... 51 7e-07
At1g12240.1 68414.m01416 beta-fructosidase (BFRUCT4) / beta-fruc... 51 7e-07
At3g13784.1 68416.m01741 beta-fructosidase, putative / beta-fruc... 46 3e-05
At4g14900.1 68417.m02288 hydroxyproline-rich glycoprotein family... 30 1.4
At4g11560.1 68417.m01853 bromo-adjacent homology (BAH) domain-co... 28 5.6
At1g26860.1 68414.m03276 hypothetical protein 28 5.6
At1g12230.1 68414.m01415 transaldolase, putative similar to Swis... 28 5.6
>At2g36190.1 68415.m04442 beta-fructosidase, putative /
beta-fructofuranosidase, putative similar to
beta-fructofuranosidase GI:18324 from [Daucus carota]
Length = 591
Score = 57.6 bits (133), Expect = 8e-09
Identities = 22/47 (46%), Positives = 30/47 (63%)
Frame = +1
Query: 103 EEYIADKKAEINPRYRPHYHISPPVGWMNDPNGFSYYKEKFHLFYQF 243
EE ++ +N +RP +H PP W+NDPNG YYK +HLFYQ+
Sbjct: 35 EELQSESVESVNHLHRPSFHFQPPKHWINDPNGPVYYKGLYHLFYQY 81
Score = 56.0 bits (129), Expect = 2e-08
Identities = 32/73 (43%), Positives = 37/73 (50%), Gaps = 2/73 (2%)
Frame = +2
Query: 506 PSYVPDNSADFRDPKI-WKFKD-HWYVVIGSSSNKRGRVLLYRSGDLFNWEFLSVLGESD 679
P Y + SA FRDP W KD HW V+GS +RG +YRS D +W S
Sbjct: 177 PDYTMNGSA-FRDPTTAWFSKDGHWRTVVGSKRKRRGIAYIYRSRDFKHWVKAKHPVHSK 235
Query: 680 GDMGYMWECPDLF 718
G MWECPD F
Sbjct: 236 QSTG-MWECPDFF 247
Score = 36.7 bits (81), Expect = 0.016
Identities = 19/54 (35%), Positives = 26/54 (48%), Gaps = 5/54 (9%)
Frame = +3
Query: 261 WGPMHWGHVSSSNLIDWEHLPTALIPETEMCFSGG-----AVVHGDDLVLLYTG 407
WG + W H S +L++WE L AL P G +V G ++LYTG
Sbjct: 88 WGNIIWAHSVSKDLVNWEALEPALSPSKWFDIGGTWSGSITIVPGKGPIILYTG 141
>At1g55120.1 68414.m06295 beta-fructosidase, putative /
beta-fructofuranosidase, putative similar to
beta-fructofuranosidase GI:402740
Length = 594
Score = 57.6 bits (133), Expect = 8e-09
Identities = 24/48 (50%), Positives = 28/48 (58%)
Frame = +1
Query: 115 ADKKAEINPRYRPHYHISPPVGWMNDPNGFSYYKEKFHLFYQFYPYEA 258
A ++N YR YH P WMNDPNG YK +HLFYQ+ PY A
Sbjct: 28 ASSHQDLNQPYRTGYHFQPLKNWMNDPNGPMIYKGIYHLFYQYNPYGA 75
Score = 54.8 bits (126), Expect = 6e-08
Identities = 30/68 (44%), Positives = 40/68 (58%), Gaps = 3/68 (4%)
Frame = +2
Query: 524 NSADFRDPKI-WKFKD-HWYVVIGSSSN-KRGRVLLYRSGDLFNWEFLSVLGESDGDMGY 694
N FRDP W +D W V++GSS++ +RG +LY+S D FNW S+ D+
Sbjct: 174 NPDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWT-QSMKPLHYEDLTG 232
Query: 695 MWECPDLF 718
MWECPD F
Sbjct: 233 MWECPDFF 240
Score = 31.9 bits (69), Expect = 0.45
Identities = 17/49 (34%), Positives = 26/49 (53%), Gaps = 5/49 (10%)
Frame = +3
Query: 276 WGHVSSSNLIDWEHLPTALIP----ETEMCFSGG-AVVHGDDLVLLYTG 407
WGH +S +L++W P A P + C+SG ++ V+LYTG
Sbjct: 83 WGHSTSVDLVNWISQPPAFNPSQPSDINGCWSGSVTILPNGKPVILYTG 131
>At3g13790.1 68416.m01742 beta-fructosidase (BFRUCT1) /
beta-fructofuranosidase / cell wall invertase identical
to beta-fructofuranosidase GI:402740 from [Arabidopsis
thaliana]
Length = 584
Score = 56.4 bits (130), Expect = 2e-08
Identities = 23/52 (44%), Positives = 29/52 (55%)
Frame = +1
Query: 94 RELEEYIADKKAEINPRYRPHYHISPPVGWMNDPNGFSYYKEKFHLFYQFYP 249
+ L + K +N YR +H PP WMNDPNG YK +HLFYQ+ P
Sbjct: 34 KRLTQSTNTKSPSVNQPYRTGFHFQPPKNWMNDPNGPMIYKGIYHLFYQWNP 85
Score = 52.8 bits (121), Expect = 2e-07
Identities = 28/67 (41%), Positives = 35/67 (52%), Gaps = 2/67 (2%)
Frame = +2
Query: 524 NSADFRDPKI-WKFKDH-WYVVIGSSSNKRGRVLLYRSGDLFNWEFLSVLGESDGDMGYM 697
N++ FRDP W +D W V+IGS ++RG + Y S D WE D G M
Sbjct: 186 NASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSG-M 244
Query: 698 WECPDLF 718
WECPD F
Sbjct: 245 WECPDFF 251
Score = 41.5 bits (93), Expect = 6e-04
Identities = 20/54 (37%), Positives = 31/54 (57%), Gaps = 5/54 (9%)
Frame = +3
Query: 261 WGPMHWGHVSSSNLIDWEHLPTALIPETEM----CFSGGA-VVHGDDLVLLYTG 407
WG + W H +S++LI+W+ P A+ P C+SG A ++ V+LYTG
Sbjct: 90 WGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGCWSGSATILPNGKPVILYTG 143
>At1g62660.1 68414.m07071 beta-fructosidase (BFRUCT3) /
beta-fructofuranosidase / invertase, vacuolar identical
to beta-fructosidase GB:CAA67560 GI:1429209 [Arabidopsis
thaliana]; supported by full-length cDNA GI:14517549;
identical to cDNA Beta-fructosidase GI:3115854
Length = 648
Score = 54.0 bits (124), Expect = 1e-07
Identities = 37/99 (37%), Positives = 52/99 (52%), Gaps = 10/99 (10%)
Frame = +3
Query: 261 WGPMHWGHVSSSNLIDWEHLPTALIPETEMCFSG---GAVVHGDD--LVLLYTGRVTTDT 425
WG + WGH S +LI W +LP A++P+ +G G+ DD +V+LYTG T
Sbjct: 144 WGDIVWGHAVSKDLIHWLYLPIAMVPDQWYDANGVWTGSATFLDDGSIVMLYTG----ST 199
Query: 426 DPFYNETQYLAFSNDG-----VNFRKYEGNPVLPTCPTI 527
D F + Q LA+ D + + K+ GNPVL P I
Sbjct: 200 DEFV-QVQNLAYPEDPSDPLLLKWVKFSGNPVLVPPPGI 237
Score = 46.8 bits (106), Expect = 1e-05
Identities = 18/37 (48%), Positives = 22/37 (59%)
Frame = +1
Query: 148 RPHYHISPPVGWMNDPNGFSYYKEKFHLFYQFYPYEA 258
R +H P WMNDPNG +YK +H FYQ+ P A
Sbjct: 106 RTAFHFQPEKNWMNDPNGPLFYKGWYHFFYQYNPNAA 142
Score = 41.1 bits (92), Expect = 7e-04
Identities = 24/64 (37%), Positives = 31/64 (48%), Gaps = 2/64 (3%)
Frame = +2
Query: 533 DFRDPKI-WKFKD-HWYVVIGSSSNKRGRVLLYRSGDLFNWEFLSVLGESDGDMGYMWEC 706
DFRDP WK W + IGS N+ G L+Y + D +E L + G MWEC
Sbjct: 241 DFRDPTTAWKTSSGKWRITIGSKINRTGISLIYDTTDFKTYEKHETLLHQVPNTG-MWEC 299
Query: 707 PDLF 718
D +
Sbjct: 300 VDFY 303
>At5g11920.1 68418.m01394 glycosyl hydrolase family 32 protein
similar to fructan 1-exohydrolase IIa GI:13940209 from
[Cichorium intybus]; contains Pfam profile PF00251:
Glycosyl hydrolases family 32
Length = 550
Score = 53.6 bits (123), Expect = 1e-07
Identities = 35/76 (46%), Positives = 43/76 (56%), Gaps = 5/76 (6%)
Frame = +2
Query: 506 PSYVPDNSADFRDP-KIWKFKD-HWYVVIGSSSN--KRGRVLLYRSGDLFNWEFLSV-LG 670
PS VP N FRDP + WK +D W V+IG+ ++G +LYRS D W V L
Sbjct: 148 PSNVPFNC--FRDPTEAWKGQDGKWRVLIGAKEKDTEKGMAILYRSDDFVQWTKYPVPLL 205
Query: 671 ESDGDMGYMWECPDLF 718
ES+G MWECPD F
Sbjct: 206 ESEGTG--MWECPDFF 219
Score = 44.0 bits (99), Expect = 1e-04
Identities = 17/34 (50%), Positives = 20/34 (58%)
Frame = +1
Query: 148 RPHYHISPPVGWMNDPNGFSYYKEKFHLFYQFYP 249
R +H P W+NDPN YYK +HLFYQ P
Sbjct: 17 RTSFHFQPQRNWLNDPNAPMYYKGFYHLFYQNNP 50
Score = 38.3 bits (85), Expect = 0.005
Identities = 19/49 (38%), Positives = 28/49 (57%), Gaps = 5/49 (10%)
Frame = +3
Query: 276 WGHVSSSNLIDWEHLPTALIP----ETEMCFSGGAVVHGDDL-VLLYTG 407
WGH S ++++W L AL+P + C+SG A + D V+LYTG
Sbjct: 62 WGHSVSQDMVNWIQLEPALVPSESFDINSCWSGSATILPDGRPVILYTG 110
>At3g52600.1 68416.m05794 beta-fructosidase, putative /
beta-fructofuranosidase, putative similar to
beta-fructofuranosidase [Daucus carota] GI:18324
Length = 590
Score = 51.2 bits (117), Expect = 7e-07
Identities = 19/39 (48%), Positives = 25/39 (64%)
Frame = +1
Query: 133 INPRYRPHYHISPPVGWMNDPNGFSYYKEKFHLFYQFYP 249
++P +R YH PP W+NDPN YK +HLFYQ+ P
Sbjct: 43 VSPLHRTAYHFQPPRHWINDPNAPMLYKGVYHLFYQYNP 81
Score = 44.4 bits (100), Expect = 8e-05
Identities = 26/69 (37%), Positives = 34/69 (49%), Gaps = 3/69 (4%)
Frame = +2
Query: 521 DNSADFRDPKIWKF--KD-HWYVVIGSSSNKRGRVLLYRSGDLFNWEFLSVLGESDGDMG 691
+N + FRDP F KD +W +++GS RG +Y+S D W S G
Sbjct: 179 ENGSAFRDPTTAWFNKKDGYWRMLVGSKRKNRGIAYMYKSRDFKKWVKSKRPIHSRKKTG 238
Query: 692 YMWECPDLF 718
MWECPD F
Sbjct: 239 -MWECPDFF 246
Score = 39.1 bits (87), Expect = 0.003
Identities = 22/60 (36%), Positives = 29/60 (48%), Gaps = 5/60 (8%)
Frame = +3
Query: 261 WGPMHWGHVSSSNLIDWEHLPTALIPETEMCFSG---GAVVH--GDDLVLLYTGRVTTDT 425
WG + W H S +LI+WE L A+ P +G G+ H G V+LYTG T
Sbjct: 86 WGNIVWAHSVSKDLINWEALEPAIYPSKWFDINGTWSGSATHVPGKGPVILYTGITENQT 145
>At1g12240.1 68414.m01416 beta-fructosidase (BFRUCT4) /
beta-fructofuranosidase / invertase, vacuolar identical
to beta-fructosidase GI:1871503 from [Arabidopsis
thaliana]; contains Pfam profile PF00251:Glycosyl
hydrolases family 32; identical to cDNA
beta-fructosidase (vacuolar form) GI:1321683; similar to
SP:Q43857
Length = 664
Score = 51.2 bits (117), Expect = 7e-07
Identities = 34/96 (35%), Positives = 48/96 (50%), Gaps = 7/96 (7%)
Frame = +3
Query: 261 WGPMHWGHVSSSNLIDWEHLPTALIP----ETEMCFSGGAVVHGD-DLVLLYTGRVTTDT 425
WG + WGH S +LI W HLP A++ ++ ++G A D +V+LYTG +TD
Sbjct: 159 WGDIVWGHAVSRDLIHWVHLPIAMVADQWYDSNGVWTGSATFLPDGSIVMLYTG--STDK 216
Query: 426 DPFYNETQYLAFSNDGVNFR--KYEGNPVLPTCPTI 527
Y ND + + K+ GNPVL P I
Sbjct: 217 AVQVQNLAYPEDPNDPLLLKWVKFPGNPVLVPPPGI 252
Score = 46.8 bits (106), Expect = 1e-05
Identities = 18/37 (48%), Positives = 22/37 (59%)
Frame = +1
Query: 148 RPHYHISPPVGWMNDPNGFSYYKEKFHLFYQFYPYEA 258
R +H P WMNDPNG +YK +H FYQ+ P A
Sbjct: 121 RTAFHFQPEQNWMNDPNGPLFYKGWYHFFYQYNPNAA 157
Score = 44.4 bits (100), Expect = 8e-05
Identities = 26/64 (40%), Positives = 33/64 (51%), Gaps = 2/64 (3%)
Frame = +2
Query: 533 DFRDPKI-WKFKD-HWYVVIGSSSNKRGRVLLYRSGDLFNWEFLSVLGESDGDMGYMWEC 706
DFRDP WK + W + IGS NK G L+Y + D +E L L + G MWEC
Sbjct: 256 DFRDPTTAWKTSEGKWRITIGSKLNKTGISLVYDTIDFKTYEKLDTLLHRVPNTG-MWEC 314
Query: 707 PDLF 718
D +
Sbjct: 315 VDFY 318
>At3g13784.1 68416.m01741 beta-fructosidase, putative /
beta-fructofuranosidase, putative / cell wall invertase,
putative similar to beta-fructofuranosidase GI:402740
from [Arabidopsis thaliana]
Length = 569
Score = 45.6 bits (103), Expect = 3e-05
Identities = 27/68 (39%), Positives = 34/68 (50%), Gaps = 3/68 (4%)
Frame = +2
Query: 524 NSADFRDPKI-WKFKD-HWYVVIGSSSNKRGRVLLYRSGDLFNW-EFLSVLGESDGDMGY 694
NS+ FRDP W +D W + GS +RG +L+ S D W + L DG
Sbjct: 176 NSSAFRDPTTAWFGRDGRWRITTGSQEGRRGLAILHTSKDFVIWKQSPKPLHYHDGT--G 233
Query: 695 MWECPDLF 718
MWECPD F
Sbjct: 234 MWECPDFF 241
Score = 40.7 bits (91), Expect = 0.001
Identities = 18/36 (50%), Positives = 20/36 (55%)
Frame = +1
Query: 136 NPRYRPHYHISPPVGWMNDPNGFSYYKEKFHLFYQF 243
N YR YH PP WMN P YK +HLFYQ+
Sbjct: 39 NQPYRTGYHFQPPKNWMNGP---MIYKGIYHLFYQW 71
Score = 33.9 bits (74), Expect = 0.11
Identities = 18/49 (36%), Positives = 28/49 (57%), Gaps = 5/49 (10%)
Frame = +3
Query: 276 WGHVSSSNLIDWEHLPTALIP----ETEMCFSGG-AVVHGDDLVLLYTG 407
WGH +S++LI+W L A+ P + C+SG ++ V+LYTG
Sbjct: 85 WGHATSTDLINWITLSPAIRPSRPSDINGCWSGSVTILPNGKPVILYTG 133
>At4g14900.1 68417.m02288 hydroxyproline-rich glycoprotein family
protein
Length = 532
Score = 30.3 bits (65), Expect = 1.4
Identities = 19/56 (33%), Positives = 25/56 (44%), Gaps = 4/56 (7%)
Frame = +1
Query: 136 NPRYR-PHYHISPPVGWMNDPNGFSYYKEKFHLFYQFYPYE---AFGVRCTGVTFP 291
+P+Y P Y SPP + N + Y E H YQ P A+G C+ V P
Sbjct: 457 SPQYGVPAYTTSPPTIYSNRSPPYQYSPEAVHGSYQTSPVSYPTAYGTYCSPVAAP 512
>At4g11560.1 68417.m01853 bromo-adjacent homology (BAH)
domain-containing protein contains Pfam profile PF01426:
BAH domain
Length = 587
Score = 28.3 bits (60), Expect = 5.6
Identities = 21/61 (34%), Positives = 32/61 (52%), Gaps = 2/61 (3%)
Frame = +2
Query: 77 KMRPLSENLKNTSRIKRQKSIHGTAR--TITSLRPSAG*MTLTAFHTTRKNSTSSISFTL 250
KMR L NLKNT+ + R + ++G TI ++ P+ LTA TT+K + +
Sbjct: 429 KMRTLVFNLKNTALLAR-RLLNGELEPATILNMSPTELKEGLTADETTKKEPDDADRMQM 487
Query: 251 T 253
T
Sbjct: 488 T 488
>At1g26860.1 68414.m03276 hypothetical protein
Length = 240
Score = 28.3 bits (60), Expect = 5.6
Identities = 11/47 (23%), Positives = 26/47 (55%)
Frame = +2
Query: 584 IGSSSNKRGRVLLYRSGDLFNWEFLSVLGESDGDMGYMWECPDLFEL 724
+G+++ +R L+ + F W ++G + D+ ++ +C DL E+
Sbjct: 188 LGAANLRRSLSPLHAELEAFLWAMKCMIGADNQDVVFLTDCSDLVEM 234
>At1g12230.1 68414.m01415 transaldolase, putative similar to
Swiss-Prot:P30148 transaldolase B (EC 2.2.1.2)
[Escherichia coli O157:H7]
Length = 405
Score = 28.3 bits (60), Expect = 5.6
Identities = 14/27 (51%), Positives = 18/27 (66%), Gaps = 1/27 (3%)
Frame = +3
Query: 351 CFSGGAVVH-GDDLVLLYTGRVTTDTD 428
CF A+V+ G DLV L GRV+T+ D
Sbjct: 141 CFFNKAIVNVGGDLVKLVPGRVSTEVD 167
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,885,051
Number of Sequences: 28952
Number of extensions: 348386
Number of successful extensions: 942
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 899
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 936
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1614253080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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