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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0554
         (636 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g61600.1 68414.m06941 expressed protein                             31   0.64 
At1g10870.1 68414.m01249 ARF GTPase-activating domain-containing...    31   0.85 
At4g16080.1 68417.m02438 hypothetical protein contains Pfam prof...    29   3.4  
At5g25170.1 68418.m02984 expressed protein                             28   4.5  

>At1g61600.1 68414.m06941 expressed protein 
          Length = 421

 Score = 31.1 bits (67), Expect = 0.64
 Identities = 28/104 (26%), Positives = 46/104 (44%), Gaps = 3/104 (2%)
 Frame = +2

Query: 167 RQGCEHRTSNTCHPLRPKHPGGCYYPTRCLIPQGIFVDLLGWGTTVQGGSVSDGNLHKLE 346
           ++G E RTS + HP RP+   G     R  +P   F       +T++ GSV  G  +   
Sbjct: 172 KKGWELRTSRSLHPRRPREALGLDEELRARLPAFGFP-----VSTIRSGSVIVGEWYCPF 226

Query: 347 LIVTNKENC---REQYKGHDRVVTDNKFCAGLVRAGGRDYDNTD 469
           + V  KENC   ++  K     +T +++   +   G  D D  +
Sbjct: 227 MFV--KENCSVSQQMRKSMFYRITLSQYWERIYHCGNNDLDENN 268


>At1g10870.1 68414.m01249 ARF GTPase-activating domain-containing
           protein 
          Length = 775

 Score = 30.7 bits (66), Expect = 0.85
 Identities = 16/49 (32%), Positives = 27/49 (55%), Gaps = 1/49 (2%)
 Frame = -1

Query: 603 VNVL*DPFSERTDAGEYYGVDVIGIALSKRYEPT-SAFWKKAGAPKSVL 460
           VN++   F + TD   Y+ +D    A+ KR++PT     K++  P+S L
Sbjct: 633 VNIINTKFDDITDIDAYHHIDAAEKAVKKRHDPTVCQRIKESNEPRSCL 681


>At4g16080.1 68417.m02438 hypothetical protein contains Pfam profile
           PF03478: Protein of unknown function (DUF295)
          Length = 379

 Score = 28.7 bits (61), Expect = 3.4
 Identities = 13/26 (50%), Positives = 16/26 (61%)
 Frame = -1

Query: 390 PLYCSLQFSLLVTMSSSLWRLPSDTL 313
           P Y  L FS+LVT  +SLW L  D +
Sbjct: 212 PQYHELNFSILVTGHTSLWSLLQDNV 237


>At5g25170.1 68418.m02984 expressed protein
          Length = 218

 Score = 28.3 bits (60), Expect = 4.5
 Identities = 12/26 (46%), Positives = 16/26 (61%)
 Frame = +1

Query: 415 QILRRFGPGWWPRLRQY*LGCPCLLP 492
           Q+ RR  P W  RL ++ L C C+LP
Sbjct: 127 QLTRRSIPSWVNRLARFGLFCNCVLP 152


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,103,139
Number of Sequences: 28952
Number of extensions: 344177
Number of successful extensions: 849
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 819
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 849
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1305036432
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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