Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0547
         (470 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC11E3.15 |rpl22|SPAP8A3.01|60S ribosomal protein L22|Schizosa...    46   2e-06
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb...    27   1.4  
SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|...    27   1.9  
SPBC409.04c |mis12||kinetochore protein Mis12|Schizosaccharomyce...    26   2.5  
SPCC794.03 |||amino acid permease, unknown 13|Schizosaccharomyce...    25   4.4  
SPBC16C6.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2||...    25   4.4  

>SPAC11E3.15 |rpl22|SPAP8A3.01|60S ribosomal protein
           L22|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 117

 Score = 46.4 bits (105), Expect = 2e-06
 Identities = 24/66 (36%), Positives = 35/66 (53%), Gaps = 1/66 (1%)
 Frame = +1

Query: 265 VVVARD-KTKVAITADIPFSXXXXXXXXXXXXXXXXXXDWLRVVASAHDAYELRYFNINA 441
           VVV+R+  +K+A+ A I FS                  DWLRVV++    YELRY+N+  
Sbjct: 51  VVVSREGSSKIAVIAHIDFSGRYLKYLTKKFLKKHSLRDWLRVVSTKKGVYELRYYNVVV 110

Query: 442 DSDNED 459
            +D E+
Sbjct: 111 GNDEEE 116



 Score = 44.0 bits (99), Expect = 1e-05
 Identities = 19/36 (52%), Positives = 23/36 (63%)
 Frame = +2

Query: 146 KFTIDCTHPAEDSILDVGNFEKYLKEHVKVEGKTNN 253
           K+ ID T    D I DV  FEKYL + +KV+GKT N
Sbjct: 11  KYIIDATAAVNDKIFDVAAFEKYLIDRIKVDGKTGN 46


>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1778

 Score = 27.1 bits (57), Expect = 1.4
 Identities = 12/30 (40%), Positives = 20/30 (66%)
 Frame = +2

Query: 191  DVGNFEKYLKEHVKVEGKTNNQAITLSSPG 280
            + GN +KY + ++KV GK N+  +T  +PG
Sbjct: 955  EAGNLKKYDQPNLKVSGK-NDSFVTHHTPG 983


>SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 1064

 Score = 26.6 bits (56), Expect = 1.9
 Identities = 13/50 (26%), Positives = 26/50 (52%), Gaps = 1/50 (2%)
 Frame = -3

Query: 213 YFSKLPTSKILSSAGCVQSIVNFKLIFR-LMPLPRILPPFTPFLASLLVQ 67
           ++ +    ++    G +++I    ++F  L  L RI+ PFTPF+   + Q
Sbjct: 729 WYIRFNRRRLKGEDGEIETINALNVLFEVLFTLVRIMGPFTPFITENIYQ 778


>SPBC409.04c |mis12||kinetochore protein Mis12|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 259

 Score = 26.2 bits (55), Expect = 2.5
 Identities = 15/47 (31%), Positives = 24/47 (51%)
 Frame = +3

Query: 231 KLRAKQITKQSRCRRQG*DESRYHRRHSFFKEVPEVFNKTLPQEEQF 371
           +LR     + SR R +  +  ++  R SF  +VPE +  TLP+   F
Sbjct: 125 QLRRDLRMRLSRKREKKTEIEKHLERISFLNKVPENWQVTLPETTDF 171


>SPCC794.03 |||amino acid permease, unknown 13|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 554

 Score = 25.4 bits (53), Expect = 4.4
 Identities = 8/22 (36%), Positives = 16/22 (72%)
 Frame = -1

Query: 341 KYFRYLFEKGMSAVIATFVLSL 276
           K+FRY+F   ++ ++  FVL++
Sbjct: 198 KWFRYIFRVSVAVILLDFVLNM 219


>SPBC16C6.04 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 350

 Score = 25.4 bits (53), Expect = 4.4
 Identities = 18/53 (33%), Positives = 30/53 (56%), Gaps = 7/53 (13%)
 Frame = +2

Query: 188 LDVGNFEKYLKEHVKVEG------KTNNQAITLS-SPGIRRKSLSPQTFLFQR 325
           LD  +F K   +H++V+        +++  I+ S  P +RRKSL P+  +FQR
Sbjct: 111 LDQRSFSKTPNKHIEVKNLKDLCSPSHSGRISKSLCPVLRRKSLLPKPKMFQR 163


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,722,912
Number of Sequences: 5004
Number of extensions: 31120
Number of successful extensions: 86
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 85
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 86
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 180421690
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)