Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0543
(390 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 138 4e-34
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 79 3e-16
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 60 1e-10
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 46 3e-06
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 40 1e-04
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 39 3e-04
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 36 0.002
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 33 0.016
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 29 0.19
SPAP27G11.03 |||D123 family|Schizosaccharomyces pombe|chr 1|||Ma... 27 1.0
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 1.8
SPCP1E11.04c |pal1||membrane associated protein Pal1 |Schizosacc... 26 2.4
SPBC23G7.08c |rga7||GTPase activating protein Rga7|Schizosacchar... 25 3.1
SPAC3H5.11 |||NAD/NADH kinase |Schizosaccharomyces pombe|chr 1||... 25 4.1
SPAC821.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 5.5
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 25 5.5
SPBC1703.11 |||optic atrophy 3 family protein|Schizosaccharomyce... 24 7.2
SPAC4D7.10c |||SAGA complex subunit Spt20 |Schizosaccharomyces p... 24 9.5
SPBC14C8.02 |tim44||TIM23 translocase complex subunit Tim44|Schi... 24 9.5
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 24 9.5
SPBC31F10.11c |cwf4|syf3|complexed with Cdc5 protein Cwf4 |Schiz... 24 9.5
SPBC17A3.01c |tim50|SPBC8D2.21c|TIM23 translocase complex subuni... 24 9.5
SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ... 24 9.5
SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo... 24 9.5
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 138 bits (333), Expect = 4e-34
Identities = 62/69 (89%), Positives = 66/69 (95%)
Frame = +3
Query: 57 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 236
+EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Query: 237 RGILTLNTP 263
RGILTL P
Sbjct: 62 RGILTLKYP 70
Score = 81.0 bits (191), Expect = 6e-17
Identities = 33/44 (75%), Positives = 36/44 (81%)
Frame = +2
Query: 254 QYPIVHGIVTNWDDMVKIWHHNFYNELRVVPDEDPVLLTDAALN 385
+YPI HGIV NWDDM KIWHH FYNELRV P+E P LLT+A LN
Sbjct: 68 KYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLN 111
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 78.6 bits (185), Expect = 3e-16
Identities = 34/63 (53%), Positives = 45/63 (71%)
Frame = +3
Query: 75 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 254
+ +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG+L +
Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRGLLKI 71
Query: 255 NTP 263
P
Sbjct: 72 QRP 74
Score = 44.4 bits (100), Expect = 6e-06
Identities = 19/44 (43%), Positives = 29/44 (65%), Gaps = 1/44 (2%)
Frame = +2
Query: 254 QYPIVHGIVTNWDDMVKIWHHNFYN-ELRVVPDEDPVLLTDAAL 382
Q PI GI+ NW DM +IW + + + +L +P+E P+LLT+ L
Sbjct: 72 QRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPL 115
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 60.1 bits (139), Expect = 1e-10
Identities = 32/69 (46%), Positives = 43/69 (62%), Gaps = 2/69 (2%)
Frame = +3
Query: 63 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSK 236
E A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++
Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAV 61
Query: 237 RGILTLNTP 263
R +L + P
Sbjct: 62 RSLLQVKYP 70
Score = 40.3 bits (90), Expect = 1e-04
Identities = 13/44 (29%), Positives = 31/44 (70%)
Frame = +2
Query: 254 QYPIVHGIVTNWDDMVKIWHHNFYNELRVVPDEDPVLLTDAALN 385
+YP+ +GI+ ++++M ++W + F+ +L++ P +LLT+ +N
Sbjct: 68 KYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMN 111
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 45.6 bits (103), Expect = 3e-06
Identities = 18/43 (41%), Positives = 25/43 (58%)
Frame = +2
Query: 257 YPIVHGIVTNWDDMVKIWHHNFYNELRVVPDEDPVLLTDAALN 385
YPI HG + NWD M + W + + LR P++ LLT+ LN
Sbjct: 92 YPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLN 134
Score = 42.7 bits (96), Expect = 2e-05
Identities = 20/52 (38%), Positives = 32/52 (61%), Gaps = 2/52 (3%)
Frame = +3
Query: 75 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSYVGDE 224
+++DNG+G K G+AG+DAP VFP+++ R G G + K SY+ +
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASK 58
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 40.3 bits (90), Expect = 1e-04
Identities = 16/34 (47%), Positives = 24/34 (70%)
Frame = +3
Query: 60 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 161
+EV+A+V+D GS + GF+G+D P+ V PS G
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42
Score = 36.3 bits (80), Expect = 0.002
Identities = 22/66 (33%), Positives = 32/66 (48%), Gaps = 2/66 (3%)
Frame = +2
Query: 182 DGRH--GTERLVRRRRGTEQKRYPDPQYPIVHGIVTNWDDMVKIWHHNFYNELRVVPDED 355
DGR G E + + G E K I +G V NWD V +W + +L+ P E
Sbjct: 46 DGRRLFGEEYIYKSNPGMEIKN------AIRNGWVENWDVTVDLWRYGLEQQLKTNPLEH 99
Query: 356 PVLLTD 373
P+L+T+
Sbjct: 100 PILITE 105
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 38.7 bits (86), Expect = 3e-04
Identities = 20/57 (35%), Positives = 33/57 (57%)
Frame = +3
Query: 60 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ 230
EE+ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQ 58
Score = 29.5 bits (63), Expect = 0.19
Identities = 10/39 (25%), Positives = 22/39 (56%)
Frame = +2
Query: 269 HGIVTNWDDMVKIWHHNFYNELRVVPDEDPVLLTDAALN 385
+GI+ +W+ + W +L+V P E +++T+ + N
Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWN 112
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 35.9 bits (79), Expect = 0.002
Identities = 15/32 (46%), Positives = 22/32 (68%)
Frame = +3
Query: 75 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 170
LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 33.1 bits (72), Expect = 0.016
Identities = 17/48 (35%), Positives = 26/48 (54%)
Frame = +3
Query: 120 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLNTP 263
G+ PRA F ++ P H G+++ M KD G+E S +G + TP
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 29.5 bits (63), Expect = 0.19
Identities = 19/55 (34%), Positives = 23/55 (41%), Gaps = 1/55 (1%)
Frame = -3
Query: 217 PTYESFCPM-PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 56
PT S P+ PT+ P P N S P PL+TTN TS+S
Sbjct: 401 PTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455
>SPAP27G11.03 |||D123 family|Schizosaccharomyces pombe|chr
1|||Manual
Length = 319
Score = 27.1 bits (57), Expect = 1.0
Identities = 9/33 (27%), Positives = 19/33 (57%)
Frame = +2
Query: 248 DPQYPIVHGIVTNWDDMVKIWHHNFYNELRVVP 346
+P YP G++ +W ++ + N E+R++P
Sbjct: 241 NPFYPRTDGLLFSWSELESMNSENMKPEIRLIP 273
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.2 bits (55), Expect = 1.8
Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 2/41 (4%)
Frame = -3
Query: 220 SPTYESFC--PMPTITP*WRGLPTIEGNTARGASSPAKPAL 104
S T +SF P+PT P LPT NT + P+ PAL
Sbjct: 64 SNTPKSFAAPPVPTGAP---SLPTSSNNTQQAEERPSMPAL 101
>SPCP1E11.04c |pal1||membrane associated protein Pal1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 425
Score = 25.8 bits (54), Expect = 2.4
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -1
Query: 114 NRPCTYRSHCLLPTRQLP 61
NRP YRSH + TR+ P
Sbjct: 323 NRPSNYRSHSSMGTRRSP 340
>SPBC23G7.08c |rga7||GTPase activating protein
Rga7|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 25.4 bits (53), Expect = 3.1
Identities = 20/64 (31%), Positives = 33/64 (51%), Gaps = 1/64 (1%)
Frame = -3
Query: 244 IPLLLCASSPTYESFCPMPTI-TP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAA 68
IP L ++P +F P P++ +P + T +TA ++SP L P + TN +
Sbjct: 363 IPSPLQNTNPAPSTF-PNPSVASPAFPNSSTSNPSTAPASASPLASTLK-PSTANDTNGS 420
Query: 67 TSSS 56
+SSS
Sbjct: 421 SSSS 424
>SPAC3H5.11 |||NAD/NADH kinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 393
Score = 25.0 bits (52), Expect = 4.1
Identities = 19/64 (29%), Positives = 29/64 (45%), Gaps = 5/64 (7%)
Frame = +2
Query: 143 IPLDRRKAPPSGCDGRHGTERLVRRRRGTEQKRYPDPQYPIVHGIV--TNWDDMVK---I 307
IP D +++ DGR+ TE L RYP +P VH +W + +K +
Sbjct: 333 IPQDAQQSSWISFDGRNRTELLPGDYLTVRISRYP---FPTVHSTEEDADWFESIKRTLM 389
Query: 308 WHHN 319
W+ N
Sbjct: 390 WNQN 393
>SPAC821.03c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 485
Score = 24.6 bits (51), Expect = 5.5
Identities = 17/56 (30%), Positives = 25/56 (44%), Gaps = 5/56 (8%)
Frame = +3
Query: 87 NGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMG-----QKDSYVGDEAQSKR 239
N +GM + R F S VG + VG Q+DSY+ D++ S+R
Sbjct: 318 NATGMYGVSKGHSSSTRRPFFSDVGSSQPAEEFVGSSSSHGRQQDSYIADDSDSER 373
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 24.6 bits (51), Expect = 5.5
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = +3
Query: 75 LVVDNGSGMCKAGFAG 122
+V+DNG+ KAGFAG
Sbjct: 8 IVLDNGAYHIKAGFAG 23
>SPBC1703.11 |||optic atrophy 3 family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 218
Score = 24.2 bits (50), Expect = 7.2
Identities = 10/31 (32%), Positives = 16/31 (51%)
Frame = -1
Query: 144 IRREEHHLLRNRPCTYRSHCLLPTRQLPRRT 52
+ RE H L +N P P++++PR T
Sbjct: 169 VERELHTLRQNTPSQNEQAEATPSKEIPRET 199
>SPAC4D7.10c |||SAGA complex subunit Spt20 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 473
Score = 23.8 bits (49), Expect = 9.5
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = -3
Query: 325 VEVMMPNLHHVIPVSDDSVYDGVLRVRI 242
+E++ P+ V SD YDG L VRI
Sbjct: 101 MELIPPDCLEVFRNSDVKFYDGCLTVRI 128
>SPBC14C8.02 |tim44||TIM23 translocase complex subunit
Tim44|Schizosaccharomyces pombe|chr 2|||Manual
Length = 427
Score = 23.8 bits (49), Expect = 9.5
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = +2
Query: 203 RLVRRRRGTEQKRYPDPQYPIVHGIVTNWDDMVKIWHHNF 322
RLVR+ + + K Y + ++PIV I D + +W F
Sbjct: 223 RLVRKIQELK-KSYQESEHPIVSSIRDMADSISGVWSRMF 261
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 23.8 bits (49), Expect = 9.5
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = +2
Query: 245 PDPQYPIVHGIVTNWDDMVKIWHHNFYNELRVVPDE 352
P+ PI G V +W+ + W H Y+ L P++
Sbjct: 108 PNEIQPIQRGRVVDWEALKAFWKH-LYSLLLKDPND 142
>SPBC31F10.11c |cwf4|syf3|complexed with Cdc5 protein Cwf4
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 674
Score = 23.8 bits (49), Expect = 9.5
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = +2
Query: 203 RLVRRRRGTEQKRYPDPQYPIVHGIVTNW 289
R+ RR E+ R ++ +VH VTNW
Sbjct: 179 RMERRYHENERARGIYERFVVVHPEVTNW 207
>SPBC17A3.01c |tim50|SPBC8D2.21c|TIM23 translocase complex subunit
Tim50 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 452
Score = 23.8 bits (49), Expect = 9.5
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = +2
Query: 215 RRRGTEQKRYPDPQYPIVHGIVTNWDDMVKIWHHNFYN 328
RR G ++K + QYP ++W + VK +NF++
Sbjct: 118 RRYGPDEKEL-EKQYPAAGYSPSDWWNRVKARTNNFFS 154
>SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr
2|||Manual
Length = 962
Score = 23.8 bits (49), Expect = 9.5
Identities = 12/37 (32%), Positives = 20/37 (54%)
Frame = -3
Query: 343 DNTELIVEVMMPNLHHVIPVSDDSVYDGVLRVRIPLL 233
+N L++E +H +P S S+Y L + IPL+
Sbjct: 662 ENQILLLESKNEVIHSSLPCSKSSLYQLSLSLCIPLI 698
>SPBC1683.09c |frp1||ferric-chelate reductase
Frp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 564
Score = 23.8 bits (49), Expect = 9.5
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = +3
Query: 180 VMVGMGQKDSYVGDEAQSKRGILT 251
+M+G +Y+G AQ KR +LT
Sbjct: 165 IMIGAIHGFAYIGLAAQGKRALLT 188
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,690,044
Number of Sequences: 5004
Number of extensions: 37531
Number of successful extensions: 149
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 134
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 147
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 128029482
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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