Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0531
(663 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 113 1e-25
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 92 3e-19
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 92 3e-19
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 89 2e-18
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 71 5e-13
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 52 5e-07
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 52 5e-07
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 49 3e-06
At5g13650.2 68418.m01585 elongation factor family protein contai... 35 0.042
At5g13650.1 68418.m01584 elongation factor family protein contai... 35 0.042
At5g61930.1 68418.m07774 expressed protein contains Pfam PF05634... 31 0.68
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 29 2.8
At2g44010.1 68415.m05472 expressed protein 28 4.8
At2g35490.1 68415.m04347 plastid-lipid associated protein PAP, p... 28 6.4
At1g27750.1 68414.m03391 ubiquitin system component Cue domain-c... 28 6.4
At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /... 27 8.4
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 113 bits (271), Expect = 1e-25
Identities = 51/82 (62%), Positives = 66/82 (80%)
Frame = +2
Query: 11 RRCLYACLLTAQPRLMEPVNLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVK 190
RR +YA +TA+PRL+EPV + EIQ PE A+GGIY VLN++RGHVFEE Q GTP++ +K
Sbjct: 711 RRVIYASQITAKPRLLEPVYMVEIQAPEGALGGIYSVLNQKRGHVFEEMQRPGTPLYNIK 770
Query: 191 AYLPVNESFGFTADLRSNTGGR 256
AYLPV ESFGF++ LR+ T G+
Sbjct: 771 AYLPVVESFGFSSQLRAATSGQ 792
Score = 58.4 bits (135), Expect = 4e-09
Identities = 23/41 (56%), Positives = 31/41 (75%)
Frame = +1
Query: 256 AFPQCVFDHWQVLPGDPCEPQSKPYNVVQETRKRKGLKEGL 378
AFPQCVFDHW+++ DP EP ++ +V + RKRKGLKE +
Sbjct: 793 AFPQCVFDHWEMMSSDPLEPGTQASVLVADIRKRKGLKEAM 833
>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 91.9 bits (218), Expect = 3e-19
Identities = 47/82 (57%), Positives = 54/82 (65%)
Frame = +2
Query: 11 RRCLYACLLTAQPRLMEPVNLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVK 190
RR Y+ L A PRLMEPV EIQ P V IY VL+RRRGHV + GTP +IVK
Sbjct: 827 RRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVK 886
Query: 191 AYLPVNESFGFTADLRSNTGGR 256
A+LPV ESFGF DLR +T G+
Sbjct: 887 AFLPVIESFGFETDLRYHTQGQ 908
Score = 32.7 bits (71), Expect = 0.22
Identities = 19/51 (37%), Positives = 27/51 (52%), Gaps = 12/51 (23%)
Frame = +1
Query: 256 AFPQCVFDHWQVLPGDPCEP--QSKPY----------NVVQETRKRKGLKE 372
AF VFDHW ++PGDP + Q +P + +TR+RKG+ E
Sbjct: 909 AFCLSVFDHWAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRKGMSE 959
>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 91.9 bits (218), Expect = 3e-19
Identities = 47/82 (57%), Positives = 54/82 (65%)
Frame = +2
Query: 11 RRCLYACLLTAQPRLMEPVNLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVK 190
RR Y+ L A PRLMEPV EIQ P V IY VL+RRRGHV + GTP +IVK
Sbjct: 827 RRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVK 886
Query: 191 AYLPVNESFGFTADLRSNTGGR 256
A+LPV ESFGF DLR +T G+
Sbjct: 887 AFLPVIESFGFETDLRYHTQGQ 908
Score = 32.7 bits (71), Expect = 0.22
Identities = 19/51 (37%), Positives = 27/51 (52%), Gaps = 12/51 (23%)
Frame = +1
Query: 256 AFPQCVFDHWQVLPGDPCEP--QSKPY----------NVVQETRKRKGLKE 372
AF VFDHW ++PGDP + Q +P + +TR+RKG+ E
Sbjct: 909 AFCLSVFDHWAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRKGMSE 959
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 89.4 bits (212), Expect = 2e-18
Identities = 46/82 (56%), Positives = 54/82 (65%)
Frame = +2
Query: 11 RRCLYACLLTAQPRLMEPVNLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVK 190
RR Y+ L A PRLMEPV EIQ P V IY VL+RRRG+V + GTP +IVK
Sbjct: 813 RRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGYVTSDVPQPGTPAYIVK 872
Query: 191 AYLPVNESFGFTADLRSNTGGR 256
A+LPV ESFGF DLR +T G+
Sbjct: 873 AFLPVIESFGFETDLRYHTQGQ 894
Score = 32.7 bits (71), Expect = 0.22
Identities = 19/51 (37%), Positives = 27/51 (52%), Gaps = 12/51 (23%)
Frame = +1
Query: 256 AFPQCVFDHWQVLPGDPCEP--QSKPY----------NVVQETRKRKGLKE 372
AF VFDHW ++PGDP + Q +P + +TR+RKG+ E
Sbjct: 895 AFCLSVFDHWAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRKGMSE 945
>At3g22980.1 68416.m02898 elongation factor Tu family protein similar
to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 71.3 bits (167), Expect = 5e-13
Identities = 33/86 (38%), Positives = 49/86 (56%)
Frame = +2
Query: 5 ATRRCLYACLLTAQPRLMEPVNLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFI 184
A + A +L PR++E + CE+ +G +Y VL+RRR + +E G+ +F
Sbjct: 858 AVKDACRAAVLQTNPRIVEAMYFCELNTAPEYLGPMYAVLSRRRARILKEEMQEGSSLFT 917
Query: 185 VKAYLPVNESFGFTADLRSNTGGRPS 262
V AY+PV+ESFGF +LR T G S
Sbjct: 918 VHAYVPVSESFGFADELRKGTSGGAS 943
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 51.6 bits (118), Expect = 5e-07
Identities = 25/76 (32%), Positives = 42/76 (55%)
Frame = +2
Query: 29 CLLTAQPRLMEPVNLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVN 208
C A+P ++EPV L E++ P G + G +N+R+G + Q ++ A +P+N
Sbjct: 649 CYTAARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLN 706
Query: 209 ESFGFTADLRSNTGGR 256
FG++ LRS T G+
Sbjct: 707 NMFGYSTSLRSMTQGK 722
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 51.6 bits (118), Expect = 5e-07
Identities = 25/76 (32%), Positives = 42/76 (55%)
Frame = +2
Query: 29 CLLTAQPRLMEPVNLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVN 208
C A+P ++EPV L E++ P G + G +N+R+G + Q ++ A +P+N
Sbjct: 649 CYTAARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLN 706
Query: 209 ESFGFTADLRSNTGGR 256
FG++ LRS T G+
Sbjct: 707 NMFGYSTSLRSMTQGK 722
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 48.8 bits (111), Expect = 3e-06
Identities = 26/74 (35%), Positives = 40/74 (54%)
Frame = +2
Query: 41 AQPRLMEPVNLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFG 220
A PR++EP+ E+ PE +G + G LN RRG + G + +V + +P+ E F
Sbjct: 683 AGPRMLEPIMRVEVVTPEEHLGDVIGDLNSRRGQINSFGDKPG-GLKVVDSLVPLAEMFQ 741
Query: 221 FTADLRSNTGGRPS 262
+ + LR T GR S
Sbjct: 742 YVSTLRGMTKGRAS 755
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 35.1 bits (77), Expect = 0.042
Identities = 18/80 (22%), Positives = 37/80 (46%), Gaps = 1/80 (1%)
Frame = +2
Query: 50 RLMEPVNLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGF-T 226
+L+EP + ++ PE +G + +L +RRG +F+ V ++ +P G
Sbjct: 473 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRN 532
Query: 227 ADLRSNTGGRPSRSAYSTIG 286
A L ++ G + + + G
Sbjct: 533 AILTASRGTAILNTVFDSYG 552
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 35.1 bits (77), Expect = 0.042
Identities = 18/80 (22%), Positives = 37/80 (46%), Gaps = 1/80 (1%)
Frame = +2
Query: 50 RLMEPVNLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGF-T 226
+L+EP + ++ PE +G + +L +RRG +F+ V ++ +P G
Sbjct: 472 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRN 531
Query: 227 ADLRSNTGGRPSRSAYSTIG 286
A L ++ G + + + G
Sbjct: 532 AILTASRGTAILNTVFDSYG 551
>At5g61930.1 68418.m07774 expressed protein contains Pfam PF05634:
Arabidopsis thaliana protein of unknown function
(DUF794)
Length = 340
Score = 31.1 bits (67), Expect = 0.68
Identities = 16/33 (48%), Positives = 18/33 (54%)
Frame = -3
Query: 343 PVQRCRACSEVRTGLQGGPANGRIRTAGRPSAG 245
PV RCR C+EV G QG IRT P +G
Sbjct: 62 PVHRCRLCAEVHIGKQG----HEIRTCTGPGSG 90
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 29.1 bits (62), Expect = 2.8
Identities = 21/69 (30%), Positives = 32/69 (46%), Gaps = 1/69 (1%)
Frame = +2
Query: 59 EPVNLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNE-SFGFTADL 235
EP + I P VG + + + RRG E + + +F+ K LP+ E F +L
Sbjct: 466 EPTVIATIILPSEYVGAVINLCSDRRGQQLEYTFIDAQRVFL-KYQLPLREIVVDFYDEL 524
Query: 236 RSNTGGRPS 262
+S T G S
Sbjct: 525 KSITSGYAS 533
>At2g44010.1 68415.m05472 expressed protein
Length = 101
Score = 28.3 bits (60), Expect = 4.8
Identities = 14/37 (37%), Positives = 19/37 (51%)
Frame = +2
Query: 209 ESFGFTADLRSNTGGRPSRSAYSTIGRSSLETRANLR 319
ES+ F +D+ N GR R +S SSL +R R
Sbjct: 32 ESYDFASDIEMNVDGRHHRRGWSVGDFSSLSSRETKR 68
>At2g35490.1 68415.m04347 plastid-lipid associated protein PAP,
putative similar to plastid-lipid associated protein
PAP3 [Brassica rapa] GI:14248552; contains Pfam profile
PF04755: PAP_fibrillin
Length = 376
Score = 27.9 bits (59), Expect = 6.4
Identities = 12/22 (54%), Positives = 13/22 (59%)
Frame = +1
Query: 271 VFDHWQVLPGDPCEPQSKPYNV 336
V D W PGD EP S+P NV
Sbjct: 71 VTDEWGEKPGDANEPDSQPDNV 92
>At1g27750.1 68414.m03391 ubiquitin system component Cue
domain-containing protein very low similarity to ASC-1
complex subunit P100 [Homo sapiens] GI:12061187;
contains Pfam profile PF02845: CUE domain
Length = 1973
Score = 27.9 bits (59), Expect = 6.4
Identities = 13/26 (50%), Positives = 14/26 (53%)
Frame = +3
Query: 228 PICVPTPADGLPAVRIRPLAGPPWRP 305
P CVP PA G PAV P + P P
Sbjct: 753 PPCVPIPAPGQPAVPATPTSQIPPSP 778
>At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein weak
similarity to polygalacturonase PG1 [Glycine max]
GI:5669846; contains PF00295: Glycosyl hydrolases family
28
Length = 444
Score = 27.5 bits (58), Expect = 8.4
Identities = 11/22 (50%), Positives = 17/22 (77%), Gaps = 1/22 (4%)
Frame = +1
Query: 133 SWSRFRRVPGGRY-TYVHCEGL 195
S+ R R +PGGRY +++H +GL
Sbjct: 123 SYGRGRELPGGRYMSFIHGDGL 144
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,041,274
Number of Sequences: 28952
Number of extensions: 296833
Number of successful extensions: 721
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 696
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 716
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1393347168
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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