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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0527
         (446 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g38200.1 68417.m05392 guanine nucleotide exchange family prot...    30   0.62 
At4g35380.1 68417.m05026 guanine nucleotide exchange family prot...    28   2.5  
At5g28970.1 68418.m03584 Ulp1 protease family protein contains P...    28   3.3  
At4g12880.1 68417.m02016 plastocyanin-like domain-containing pro...    27   7.6  
At3g13990.1 68416.m01766 hydroxyproline-rich glycoprotein family...    27   7.6  

>At4g38200.1 68417.m05392 guanine nucleotide exchange family protein
           similar to Brefeldin A-inhibited guanine
           nucleotide-exchange protein 2 [Homo sapiens] SP|Q9Y6D5;
           contains Pfam profile PF01369: Sec7 domain
          Length = 1698

 Score = 30.3 bits (65), Expect = 0.62
 Identities = 23/76 (30%), Positives = 38/76 (50%)
 Frame = +1

Query: 208 TFFHMTMGNFLRGTKICVKLRLATKWMILFHHTLLSSVLL*IKTVEVKDENILICSHKYC 387
           T +++ +G F    +IC K  LA   +I+F  +  +S+   +KTV V D  +L  + K  
Sbjct: 164 TCYNVYLGGFNGTNQICAKSVLAQIMLIVFTRSEANSMDASLKTVNVND--LLAITDKNV 221

Query: 388 KLDTSI**YICSILIN 435
               S+  +IC   IN
Sbjct: 222 NEGNSV--HICQGFIN 235


>At4g35380.1 68417.m05026 guanine nucleotide exchange family protein
           similar to guanine nucleotide exchange factor [Homo
           sapiens] GI:5456754; contains Pfam profile PF01369: Sec7
           domain
          Length = 1706

 Score = 28.3 bits (60), Expect = 2.5
 Identities = 19/76 (25%), Positives = 38/76 (50%)
 Frame = +1

Query: 208 TFFHMTMGNFLRGTKICVKLRLATKWMILFHHTLLSSVLL*IKTVEVKDENILICSHKYC 387
           T +++ +G     T+IC K  LA   +++F  +   S+ + +KT+ V +  +L  + K  
Sbjct: 165 TCYNIYLGGLSGTTQICAKSVLAQMMLVIFTRSEEDSLDVSVKTIYVNE--LLTFTDKSV 222

Query: 388 KLDTSI**YICSILIN 435
              +S+  Y C   +N
Sbjct: 223 NEGSSV--YFCQGFVN 236


>At5g28970.1 68418.m03584 Ulp1 protease family protein contains Pfam
           profile PF02902: Ulp1 protease family, C-terminal
           catalytic domain
          Length = 1172

 Score = 27.9 bits (59), Expect = 3.3
 Identities = 15/43 (34%), Positives = 20/43 (46%)
 Frame = +1

Query: 196 SSGNTFFHMTMGNFLRGTKICVKLRLATKWMILFHHTLLSSVL 324
           SS     H+T   FL   K  VKL    +W+   H  +L S+L
Sbjct: 829 SSSPNIEHLTKCGFLISNKFFVKLAKPQQWVSTLHMEVLVSLL 871


>At4g12880.1 68417.m02016 plastocyanin-like domain-containing
           protein
          Length = 141

 Score = 26.6 bits (56), Expect = 7.6
 Identities = 12/38 (31%), Positives = 20/38 (52%)
 Frame = -3

Query: 264 FHTDLSSS*EISHCHVKKCVSRRPIR*L*KGVRDQHIP 151
           F+ D  +  E++    +KC+S RPIR   +G     +P
Sbjct: 62  FYRDQHNILEVNKADYEKCISNRPIRNYTRGAGRDIVP 99


>At3g13990.1 68416.m01766 hydroxyproline-rich glycoprotein family
           protein
          Length = 883

 Score = 26.6 bits (56), Expect = 7.6
 Identities = 12/33 (36%), Positives = 18/33 (54%), Gaps = 1/33 (3%)
 Frame = +3

Query: 15  KQTSDPAY-PEMVIVGINKNGVSLVHPQSRNSY 110
           +Q   P Y P+M + G + N  +  HP + NSY
Sbjct: 665 RQFLSPVYVPQMPMPGYSGNPAAYAHPSNGNSY 697


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,229,496
Number of Sequences: 28952
Number of extensions: 179070
Number of successful extensions: 312
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 304
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 312
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 722638680
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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