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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0517
         (762 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_26408| Best HMM Match : Lipase (HMM E-Value=0)                      63   2e-10
SB_43441| Best HMM Match : Lipase (HMM E-Value=8.19998e-41)            59   3e-09
SB_7713| Best HMM Match : Lipase (HMM E-Value=0.0064)                  58   8e-09
SB_19823| Best HMM Match : No HMM Matches (HMM E-Value=.)              56   2e-08
SB_43904| Best HMM Match : Lipase (HMM E-Value=5e-07)                  51   1e-06
SB_58542| Best HMM Match : No HMM Matches (HMM E-Value=.)              31   1.4  
SB_34654| Best HMM Match : zf-C2H2 (HMM E-Value=8e-31)                 31   1.4  
SB_22961| Best HMM Match : Abhydrolase_1 (HMM E-Value=0.043)           30   2.4  
SB_5209| Best HMM Match : No HMM Matches (HMM E-Value=.)               30   2.4  
SB_6080| Best HMM Match : No HMM Matches (HMM E-Value=.)               29   3.1  
SB_3297| Best HMM Match : No HMM Matches (HMM E-Value=.)               29   4.1  
SB_31241| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   4.1  
SB_27171| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   5.5  
SB_18788| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   9.5  

>SB_26408| Best HMM Match : Lipase (HMM E-Value=0)
          Length = 714

 Score = 63.3 bits (147), Expect = 2e-10
 Identities = 36/93 (38%), Positives = 54/93 (58%), Gaps = 7/93 (7%)
 Frame = +2

Query: 254 NAFLRSGNYNVIVVDWSSFSLSTYSTAVMAVTGVGSSIATFLKNLK----LPLNKVHIVG 421
           N  L  G++NVI+VDW   +   ++ AV     VG+  A  L+ L+      L  VH++G
Sbjct: 136 NELLWEGDFNVIIVDWMRGAWFPFTRAVANTRLVGAQTARLLQILEERSGRKLAYVHVIG 195

Query: 422 FNLGAHVAGVTGRNLEGK---VARITGLDPSAV 511
           F+ GAHVAG  GR ++ +   + RIT LDP+A+
Sbjct: 196 FSFGAHVAGYVGRRMKKRGRMIDRITALDPAAM 228



 Score = 50.4 bits (115), Expect = 2e-06
 Identities = 32/86 (37%), Positives = 42/86 (48%), Gaps = 9/86 (10%)
 Frame = +1

Query: 529 LRLGTNDAQYVEVIHTDGSGVNKNGLGIAIGHIDFFVNGRLVQPGCTN--------NLCS 684
           +RL T+DA +V+VIHT        G+   IGH DF+ NG   QPGC N          C 
Sbjct: 237 VRLDTSDALFVDVIHTSAD----YGITSTIGHADFYPNGGKKQPGCDNFFRGFSSYLFCG 292

Query: 685 HNRAYEVFAATI-THGKHYGNQCSTE 759
           H RA  +F  ++ T    Y   C +E
Sbjct: 293 HKRAPALFTTSLYTKTPLYSYPCRSE 318


>SB_43441| Best HMM Match : Lipase (HMM E-Value=8.19998e-41)
          Length = 291

 Score = 59.3 bits (137), Expect = 3e-09
 Identities = 37/94 (39%), Positives = 56/94 (59%), Gaps = 8/94 (8%)
 Frame = +2

Query: 263 LRSGNYNVIVVDWSSFSLSTYSTAVMAVTGVGSSIATFLKN-LKLPLNK----VHIVGFN 427
           LR  + NVI+VDW   +   Y  AV     VG+ +A F+K  L L  ++     H +GF+
Sbjct: 120 LRKSDDNVIIVDWIRGAKIPYVRAVANTRLVGAQVAAFMKTILSLSGSREGGAFHSIGFS 179

Query: 428 LGAHVAGVTGRNLE--GK-VARITGLDPSAVIGK 520
           LGAH++G  G+ L+  G+ + RITGLDP+ ++ K
Sbjct: 180 LGAHISGYVGQRLKRIGRHLDRITGLDPATLMFK 213



 Score = 37.9 bits (84), Expect = 0.009
 Identities = 25/73 (34%), Positives = 36/73 (49%), Gaps = 2/73 (2%)
 Frame = +1

Query: 457 KKFGRQSSKNYGSRPFGCDWENNV--LRLGTNDAQYVEVIHTDGSGVNKNGLGIAIGHID 630
           K+ GR   +  G  P    ++     +RL   DAQ+V+VIHT        G+    GH+D
Sbjct: 193 KRIGRHLDRITGLDPATLMFKGEAPDVRLDRLDAQFVDVIHTS----YVFGITAPHGHMD 248

Query: 631 FFVNGRLVQPGCT 669
           F+ NG   Q GC+
Sbjct: 249 FYPNGGTSQRGCS 261


>SB_7713| Best HMM Match : Lipase (HMM E-Value=0.0064)
          Length = 131

 Score = 58.0 bits (134), Expect = 8e-09
 Identities = 32/76 (42%), Positives = 43/76 (56%), Gaps = 6/76 (7%)
 Frame = +2

Query: 281 NVIVVDWSSFS--LSTYSTAVMAVTGVGSSIATFLKNLK----LPLNKVHIVGFNLGAHV 442
           NVI VDW S +  L+ Y  A      VG+ +A  +  ++      L +VH++G +LGAHV
Sbjct: 55  NVITVDWQSGADGLNLYHVAAGNTRVVGAQLAELITTIQRVFDFDLRRVHLIGHSLGAHV 114

Query: 443 AGVTGRNLEGKVARIT 490
           AG  G  L GKV RIT
Sbjct: 115 AGYAGERLSGKVGRIT 130


>SB_19823| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 940

 Score = 56.4 bits (130), Expect = 2e-08
 Identities = 32/93 (34%), Positives = 49/93 (52%), Gaps = 7/93 (7%)
 Frame = +2

Query: 254 NAFLRSGNYNVIVVDWSSFSLSTYSTAVMAVTGVGSSIATFLKNLKLPLNKV----HIVG 421
           +A ++  + NVI  DWS  +   Y  A      VG+ I   +K L           ++VG
Sbjct: 634 HALIKQEDANVITTDWSRGATIPYEQATANTRMVGAQITELIKFLNNQTGNTPASFYLVG 693

Query: 422 FNLGAHVAGVTGRNLE---GKVARITGLDPSAV 511
           F+LGAH++G  GR +     K+ RITGLDP+++
Sbjct: 694 FSLGAHISGYVGRRIAKTGQKLNRITGLDPASI 726



 Score = 50.4 bits (115), Expect = 2e-06
 Identities = 29/73 (39%), Positives = 41/73 (56%), Gaps = 8/73 (10%)
 Frame = +1

Query: 529 LRLGTNDAQYVEVIHTDGSGVNKNGLGIAIGHIDFFVNGRLVQPGCTNNL--------CS 684
           +RL  +DA +V+V+HTD   ++  G     GHIDF+ NG   QPGC + L        C 
Sbjct: 735 VRLDPSDADFVDVMHTD---MDLAGTPTVSGHIDFYPNGGKKQPGCRDLLDGPINYVICD 791

Query: 685 HNRAYEVFAATIT 723
           H RA E +A ++T
Sbjct: 792 HMRAPEYYAESVT 804


>SB_43904| Best HMM Match : Lipase (HMM E-Value=5e-07)
          Length = 408

 Score = 50.8 bits (116), Expect = 1e-06
 Identities = 30/73 (41%), Positives = 43/73 (58%), Gaps = 8/73 (10%)
 Frame = +1

Query: 529 LRLGTNDAQYVEVIHTDGSGVNKNGLGIAIGHIDFFVNGRLVQPGC-------TNNL-CS 684
           +RL  +DA++V+V+HTD   ++  G     GHIDF+ NG   QPGC       +N L C 
Sbjct: 16  VRLDPSDAEFVDVMHTD---MDFAGTSTQSGHIDFYPNGGKNQPGCRDIADGPSNALKCD 72

Query: 685 HNRAYEVFAATIT 723
           H RA++ F  +IT
Sbjct: 73  HVRAHDYFTESIT 85


>SB_58542| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 268

 Score = 30.7 bits (66), Expect = 1.4
 Identities = 12/49 (24%), Positives = 25/49 (51%)
 Frame = +2

Query: 365 IATFLKNLKLPLNKVHIVGFNLGAHVAGVTGRNLEGKVARITGLDPSAV 511
           + +FL  + +   K H+ G ++G H+ GV       +VA +  + P+ +
Sbjct: 120 LLSFLHAVGVNKRKFHLAGISMGGHIVGVYAAQHPARVASVIMMCPAGI 168


>SB_34654| Best HMM Match : zf-C2H2 (HMM E-Value=8e-31)
          Length = 624

 Score = 30.7 bits (66), Expect = 1.4
 Identities = 16/58 (27%), Positives = 29/58 (50%)
 Frame = +3

Query: 69  RFGSLNKYYYYSNAQRNPITLTEDHFPTGNDPAAPFNNNWDIVVIIHGHSGTATTTIN 242
           R+    K Y + ++ R  +   + HF  G++PA  ++NN D+   +  H  + T  IN
Sbjct: 548 RYSGCEKSYTHPSSLRKHL---KAHFKCGDNPAGIYDNNNDLETPVPPHRESKTARIN 602


>SB_22961| Best HMM Match : Abhydrolase_1 (HMM E-Value=0.043)
          Length = 183

 Score = 29.9 bits (64), Expect = 2.4
 Identities = 12/46 (26%), Positives = 23/46 (50%)
 Frame = +2

Query: 374 FLKNLKLPLNKVHIVGFNLGAHVAGVTGRNLEGKVARITGLDPSAV 511
           FL  + +   K H+ G ++G H+ GV       +VA +  + P+ +
Sbjct: 1   FLHAVGVNKRKFHLAGISMGGHIVGVYAAQHPARVASVIMMCPAGI 46


>SB_5209| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 67

 Score = 29.9 bits (64), Expect = 2.4
 Identities = 15/31 (48%), Positives = 21/31 (67%), Gaps = 4/31 (12%)
 Frame = +2

Query: 413 IVGFNLGAHVAGVTG---RNLEG-KVARITG 493
           ++GF+LG HVAG  G   +N  G K+ RI+G
Sbjct: 21  VIGFSLGGHVAGYAGSRIKNTTGLKLGRISG 51


>SB_6080| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 2101

 Score = 29.5 bits (63), Expect = 3.1
 Identities = 14/45 (31%), Positives = 20/45 (44%)
 Frame = +1

Query: 628  DFFVNGRLVQPGCTNNLCSHNRAYEVFAATITHGKHYGNQCSTEA 762
            D++   ++   G T+  C     YE      T GK YGN+C   A
Sbjct: 1278 DYYAQCQVEDDGSTSCKCPIFCTYEYMPVCGTDGKTYGNKCEMRA 1322


>SB_3297| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 153

 Score = 29.1 bits (62), Expect = 4.1
 Identities = 17/52 (32%), Positives = 23/52 (44%)
 Frame = -2

Query: 263 KTRFNNRVDRRRSSATVTVYYYDNIPIIVKGRSRIVSSGKVVFGECNWVSLC 108
           KT  N     RR S     Y+  + P  V  RS  +    +V   C+WV+LC
Sbjct: 78  KTIINQYPAARRYSVITRYYFGVSRPFSVINRSSSIVPKGLVLILCSWVTLC 129


>SB_31241| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 254

 Score = 29.1 bits (62), Expect = 4.1
 Identities = 12/23 (52%), Positives = 17/23 (73%)
 Frame = +2

Query: 455 GRNLEGKVARITGLDPSAVIGKT 523
           GR  +GK+A I+ LDP+  +GKT
Sbjct: 116 GRTQKGKLASISTLDPAREVGKT 138


>SB_27171| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 52

 Score = 28.7 bits (61), Expect = 5.5
 Identities = 15/37 (40%), Positives = 20/37 (54%), Gaps = 3/37 (8%)
 Frame = +1

Query: 595 KNGLGIAIG---HIDFFVNGRLVQPGCTNNLCSHNRA 696
           K+  G+ IG   H+D  VNG  VQ       C+HN+A
Sbjct: 12  KSSEGMYIGADLHLDKDVNGAYVQGSFIGGFCTHNKA 48


>SB_18788| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 820

 Score = 27.9 bits (59), Expect = 9.5
 Identities = 14/28 (50%), Positives = 15/28 (53%)
 Frame = +1

Query: 487 YGSRPFGCDWENNVLRLGTNDAQYVEVI 570
           YG  P GC  E N+ RLGT  A    VI
Sbjct: 603 YGQSPGGCWMEKNLSRLGTMHAPVSMVI 630


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 24,075,364
Number of Sequences: 59808
Number of extensions: 531079
Number of successful extensions: 1578
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 1438
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1569
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 2082369341
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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