Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0516
         (703 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC16A3.12c |||triglyceride lipase-cholesterol esterase |Schizo...    37   0.003
SPCC1672.09 |||triglyceride lipase-cholesterol esterase |Schizos...    36   0.007
SPAC56E4.03 |||aromatic aminotransferase |Schizosaccharomyces po...    30   0.37 
SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces po...    28   1.1  
SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyce...    28   1.5  
SPCC1020.13c ||SPCC14G10.05|phospholipase |Schizosaccharomyces p...    28   1.5  
SPAC4H3.07c |||protein phosphatase Fmp31 |Schizosaccharomyces po...    27   2.0  
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce...    27   3.4  
SPCC16A11.12c |ubp1||ubiquitin C-terminal hydrolase Ubp1|Schizos...    26   4.5  
SPBC31F10.04c |srb4|med17|mediator complex subunit Srb4|Schizosa...    26   6.0  
SPAC144.09c |sfc2||RNA polymerase III transcription factor TFIII...    25   7.9  

>SPBC16A3.12c |||triglyceride lipase-cholesterol esterase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 443

 Score = 36.7 bits (81), Expect = 0.003
 Identities = 20/57 (35%), Positives = 33/57 (57%), Gaps = 4/57 (7%)
 Frame = +1

Query: 1   GKRRLHYIGHSQGTTVFWAMGSLRPEYNSKI---IAM-QAYAPVAYLEFNANRLLKL 159
           G+ +L+YIG SQGT    A  S+ P+ N K+   I +  AYAP  +  +  + ++K+
Sbjct: 200 GREKLNYIGFSQGTAQAMAALSINPDLNDKVNIFIGLAPAYAPKGFSNYFVDYIVKV 256



 Score = 27.5 bits (58), Expect = 2.0
 Identities = 9/19 (47%), Positives = 13/19 (68%)
 Frame = +2

Query: 632 ATFSHLDFMWGSGAKELLY 688
           A + HLDF+WG   KE ++
Sbjct: 400 AHYEHLDFLWGQDVKEEVF 418


>SPCC1672.09 |||triglyceride lipase-cholesterol esterase
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 467

 Score = 35.5 bits (78), Expect = 0.007
 Identities = 27/84 (32%), Positives = 41/84 (48%)
 Frame = +1

Query: 1   GKRRLHYIGHSQGTTVFWAMGSLRPEYNSKIIAMQAYAPVAYLEFNANRLLKLIAPHANS 180
           G+ +L YIG SQGT   +A  S+ P  N KI ++ A AP    +   NR++         
Sbjct: 212 GQTKLTYIGFSQGTAQAFASLSIHPLLNDKINSLIALAPAISPKGLHNRVVDAF------ 265

Query: 181 IEALTSLIGINELFGRSDFFTNVG 252
           ++A  S++    LFGR     + G
Sbjct: 266 VKARPSILFF--LFGRKSILPSAG 287


>SPAC56E4.03 |||aromatic aminotransferase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 474

 Score = 29.9 bits (64), Expect = 0.37
 Identities = 16/56 (28%), Positives = 30/56 (53%)
 Frame = -3

Query: 500 SWRCETSVNPPIFQTSVIISSEEIVVDELTVMRYLPDSNACGCMS*FHRQHSRIVH 333
           SW+ ++S +  +   SV +SS +  V  L+V       +    +S F ++H+RI+H
Sbjct: 65  SWKKDSSNSGKLDTVSVPMSSSDSDVLPLSVALQYGQGSGAALLSQFLKEHTRIIH 120


>SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 811

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 16/45 (35%), Positives = 24/45 (53%), Gaps = 3/45 (6%)
 Frame = +2

Query: 506 PSLITAPVFL--H-YVDDDIFADVRDVRKLQIELGRPVGMFRVPH 631
           PS+ +   FL  H Y+D   F +       ++ LG+PVG+FR  H
Sbjct: 608 PSMGSRSAFLTKHIYIDRSDFREEASSDFFRLTLGQPVGLFRASH 652


>SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 242

 Score = 27.9 bits (59), Expect = 1.5
 Identities = 12/43 (27%), Positives = 23/43 (53%), Gaps = 1/43 (2%)
 Frame = +1

Query: 13  LHYIGHSQGTTVFWAMGSLRPEYNSKII-AMQAYAPVAYLEFN 138
           LH++ +S+ T + W +  L+  Y  K+   +   AP AY + +
Sbjct: 4   LHHLKNSRSTRIVWMLEELKVPYEIKVYDRVDGRAPPAYTKLS 46


>SPCC1020.13c ||SPCC14G10.05|phospholipase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 669

 Score = 27.9 bits (59), Expect = 1.5
 Identities = 13/30 (43%), Positives = 20/30 (66%)
 Frame = +1

Query: 4   KRRLHYIGHSQGTTVFWAMGSLRPEYNSKI 93
           K R+  IGHS G+TV + + SL+P +  +I
Sbjct: 391 KGRIFIIGHSLGSTVVFDILSLQPTFVKEI 420


>SPAC4H3.07c |||protein phosphatase Fmp31 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 171

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 11/31 (35%), Positives = 16/31 (51%)
 Frame = -1

Query: 367 PSFTGNIVALCMSSLRPAKANNIFVHIAWKN 275
           P F  N+V  C S  R   A++I   + +KN
Sbjct: 123 PVFEDNVVVYCRSGRRSTTASDILTKLGYKN 153


>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 2100

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 14/39 (35%), Positives = 21/39 (53%)
 Frame = +3

Query: 264 ADGVFFQAMCTNMLFAFAGRSEDMHNATMLPVKLGHTPA 380
           A+G   +++C N+LFA    S+   N  +  VK   TPA
Sbjct: 859 AEGEIEESVCDNILFAINNLSQLNFNEKLKDVKDNLTPA 897


>SPCC16A11.12c |ubp1||ubiquitin C-terminal hydrolase
           Ubp1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 849

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 14/42 (33%), Positives = 21/42 (50%)
 Frame = -3

Query: 521 RL*EKVISWRCETSVNPPIFQTSVIISSEEIVVDELTVMRYL 396
           R+  K   W C   +    F+   I+  EEIV+D+ TV+  L
Sbjct: 625 RMESKAGKWECIKEIEVKRFE---IVEEEEIVIDDKTVIMCL 663


>SPBC31F10.04c |srb4|med17|mediator complex subunit
           Srb4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 545

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 10/19 (52%), Positives = 12/19 (63%)
 Frame = +1

Query: 580 KTTDRIGSSSWNVQSTTCN 636
           K  D IGSS+W+  S  CN
Sbjct: 273 KDRDEIGSSTWSWPSQNCN 291


>SPAC144.09c |sfc2||RNA polymerase III transcription factor
           TFIIIA|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 374

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 9/31 (29%), Positives = 16/31 (51%)
 Frame = +2

Query: 293 HKYVIRFCWTQ*RHAQCDYVACETRTYTRRH 385
           H  + + C T  +   C Y  C+ + YT++H
Sbjct: 69  HLKIHKRCHTNVKPFSCHYDGCDAQFYTQQH 99


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,913,212
Number of Sequences: 5004
Number of extensions: 58641
Number of successful extensions: 166
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 166
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 325165428
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)