BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0516 (703 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g15230.1 68415.m01737 lipase family protein similar to SP|P07... 41 0.001 At5g14180.1 68418.m01658 lipase family protein similar to SP|Q64... 31 0.56 At1g15060.1 68414.m01800 expressed protein 31 0.56 At3g23280.2 68416.m02935 zinc finger (C3HC4-type RING finger) fa... 28 5.2 At3g23280.1 68416.m02934 zinc finger (C3HC4-type RING finger) fa... 28 5.2 At3g57570.1 68416.m06410 expressed protein 28 6.9 At5g01400.1 68418.m00053 expressed protein contains low similari... 27 9.1 At2g34620.1 68415.m04253 mitochondrial transcription termination... 27 9.1 >At2g15230.1 68415.m01737 lipase family protein similar to SP|P07098 Triacylglycerol lipase, gastric precursor (EC 3.1.1.3) {Homo sapiens}; contains Pfam profile PF04083: ab-hydrolase associated lipase region Length = 393 Score = 40.7 bits (91), Expect = 0.001 Identities = 21/63 (33%), Positives = 32/63 (50%) Frame = +3 Query: 291 CTNMLFAFAGRSEDMHNATMLPVKLGHTPAGIAVRQIAHYGQLINNNFFRRYDHGRLKNW 470 CT+ L + G + NA+ + L + P +V+ I H Q+I F +YD+G KN Sbjct: 246 CTDFLTSITG-TNCCFNASKIEYYLDYEPHPSSVKNIRHLFQMIRKGTFAQYDYGYFKNL 304 Query: 471 RVY 479 R Y Sbjct: 305 RTY 307 Score = 35.1 bits (77), Expect = 0.045 Identities = 21/60 (35%), Positives = 30/60 (50%) Frame = +2 Query: 524 PVFLHYVDDDIFADVRDVRKLQIELGRPVGMFRVPHATFSHLDFMWGSGAKELLYDRTIQ 703 P+++ Y D ADV DV EL + + + H+DF+ GS AKE +Y IQ Sbjct: 325 PMWMGYGGTDGLADVTDVEHTLAELPSSPELLYLED--YGHIDFVLGSSAKEDVYKHMIQ 382 Score = 30.3 bits (65), Expect = 1.3 Identities = 18/81 (22%), Positives = 41/81 (50%), Gaps = 1/81 (1%) Frame = +1 Query: 10 RLHYIGHSQGTTVFWAMGSLRPEYNSKIIAMQAYAPVAYLE-FNANRLLKLIAPHANSIE 186 ++ +GHSQGT + +A +P + A P++YL+ A + +++ H ++ Sbjct: 159 KIFLVGHSQGTIMSFA-ALTQPHVAEMVEAAALLCPISYLDHVTAPLVERMVFMH---LD 214 Query: 187 ALTSLIGINELFGRSDFFTNV 249 + +G++++ RSD + Sbjct: 215 QMVVALGLHQINFRSDMLVKL 235 >At5g14180.1 68418.m01658 lipase family protein similar to SP|Q64194 Lysosomal acid lipase/cholesteryl ester hydrolase precursor (EC 3.1.1.13) {Rattus norvegicus}; contains Pfam profile PF04083: ab-hydrolase associated lipase region Length = 418 Score = 31.5 bits (68), Expect = 0.56 Identities = 30/117 (25%), Positives = 51/117 (43%) Frame = +1 Query: 7 RRLHYIGHSQGTTVFWAMGSLRPEYNSKIIAMQAYAPVAYLEFNANRLLKLIAPHANSIE 186 +++HY+GHS GT + +A S + + ++ + +PVAYL + + +I I Sbjct: 182 QKIHYLGHSLGTLIGFASFSEKGLVD-QVRSAAMLSPVAYL----SHMTTVI----GDIA 232 Query: 187 ALTSLIGINELFGRSDFFTNVGIDFALMAYFSRRCAQICYSLLLDAVKTCTMRLCCL 357 A T L + G +F G+ + + CY D V T + CCL Sbjct: 233 AKTFLAEATSILGWPEFNPKSGLVGDFIKAICLKAGIDCY----DLVSVITGKNCCL 285 Score = 29.9 bits (64), Expect = 1.7 Identities = 18/59 (30%), Positives = 30/59 (50%), Gaps = 2/59 (3%) Frame = +2 Query: 524 PVFLHYVDDDIFADVRDVRKLQIELG-RPVGMFRVPHAT-FSHLDFMWGSGAKELLYDR 694 P+F Y D ADV+DV L + + V ++H DF+ G AK+++Y++ Sbjct: 351 PLFFSYGGLDSLADVKDVEFLLDQFKYHDIDKMNVQFVKDYAHADFIMGVTAKDVVYNQ 409 >At1g15060.1 68414.m01800 expressed protein Length = 578 Score = 31.5 bits (68), Expect = 0.56 Identities = 23/64 (35%), Positives = 37/64 (57%), Gaps = 2/64 (3%) Frame = +1 Query: 10 RLHYIGHSQGTTVFWAMGSLRPEYNSKIIAMQAYAPVAY-LEFN-ANRLLKLIAPHANSI 183 +L IGHS G + +AM S R + + ++ A A +A +++ +N LKL+ P AN Sbjct: 356 KLFAIGHSMGGILLYAMLS-RCAFEGREPSVAAVATLASSVDYTTSNSALKLLIPLANPA 414 Query: 184 EALT 195 EAL+ Sbjct: 415 EALS 418 >At3g23280.2 68416.m02935 zinc finger (C3HC4-type RING finger) family protein / ankyrin repeat family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) and Pfam profile: PF00023 ankyrin repeat Length = 438 Score = 28.3 bits (60), Expect = 5.2 Identities = 17/35 (48%), Positives = 20/35 (57%) Frame = -1 Query: 406 CAICLTAMPAGVCPSFTGNIVALCMSSLRPAKANN 302 CAICL A VC G+ VA CMS L+ K+ N Sbjct: 387 CAICLDAPSEAVCVP-CGH-VAGCMSCLKEIKSKN 419 >At3g23280.1 68416.m02934 zinc finger (C3HC4-type RING finger) family protein / ankyrin repeat family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) and Pfam profile: PF00023 ankyrin repeat Length = 462 Score = 28.3 bits (60), Expect = 5.2 Identities = 17/35 (48%), Positives = 20/35 (57%) Frame = -1 Query: 406 CAICLTAMPAGVCPSFTGNIVALCMSSLRPAKANN 302 CAICL A VC G+ VA CMS L+ K+ N Sbjct: 411 CAICLDAPSEAVCVP-CGH-VAGCMSCLKEIKSKN 443 >At3g57570.1 68416.m06410 expressed protein Length = 1057 Score = 27.9 bits (59), Expect = 6.9 Identities = 14/30 (46%), Positives = 20/30 (66%) Frame = +1 Query: 118 VAYLEFNANRLLKLIAPHANSIEALTSLIG 207 V L F+++R+LKLI A S++ SLIG Sbjct: 587 VTGLTFDSDRVLKLIPEWARSVQNWNSLIG 616 >At5g01400.1 68418.m00053 expressed protein contains low similarity to symplekin SP:Q92797 from [Homo sapiens] Length = 1467 Score = 27.5 bits (58), Expect = 9.1 Identities = 18/76 (23%), Positives = 36/76 (47%) Frame = +1 Query: 31 SQGTTVFWAMGSLRPEYNSKIIAMQAYAPVAYLEFNANRLLKLIAPHANSIEALTSLIGI 210 +QG + W++ +RP + + + + V +LE + ++L+A S+ +T I Sbjct: 815 TQGLSAVWSLILMRPGIRNDCLNIALQSAVHHLEEIRMKAIRLVANKLYSLSFITEQI-- 872 Query: 211 NELFGRSDFFTNVGID 258 E F + F+ V D Sbjct: 873 -EEFAKDRLFSVVSDD 887 >At2g34620.1 68415.m04253 mitochondrial transcription termination factor-related / mTERF-related contains Pfam profile PF02536: mTERF Length = 303 Score = 27.5 bits (58), Expect = 9.1 Identities = 17/56 (30%), Positives = 25/56 (44%) Frame = -2 Query: 324 CVQQKRITYLCTSPGKIRHQRKIYSHVRKEVRSAE*LIYSNETCKCLNTVGMWSNK 157 C QQ + T+L T P I+ ++SH V + E CL +G+ S K Sbjct: 18 CPQQSQSTFLSTKPTTIK--TNLHSHPLFTVADQTVTLQMKEKILCLELMGIDSGK 71 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,053,486 Number of Sequences: 28952 Number of extensions: 301306 Number of successful extensions: 669 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 654 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 668 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1506636208 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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