SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0514
         (468 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual    35   0.007
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    32   0.050
SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit Pst...    28   0.62 
SPAC26A3.15c |nsp1||nucleoporin Nsp1|Schizosaccharomyces pombe|c...    28   0.82 
SPAC1B1.01 |||transcription factor Rdp1|Schizosaccharomyces pomb...    27   1.1  
SPCC553.01c ||SPCC736.01c|meiotic chromosome segregation protein...    27   1.9  
SPBC354.10 |||RNAPII degradation factor |Schizosaccharomyces pom...    27   1.9  
SPBC4F6.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr ...    26   2.5  
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1...    26   2.5  
SPAC2G11.12 |rqh1|hus2, rad12, rec9|RecQ type DNA helicase Rqh1|...    26   2.5  
SPAC29A4.08c |prp19|cwf8|ubiquitin-protein ligase E4 |Schizosacc...    26   3.3  
SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe...    25   4.4  
SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|c...    25   4.4  
SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    25   5.8  
SPAC13C5.02 |dre4||DNA replication protein Dre4|Schizosaccharomy...    25   5.8  
SPBC28E12.05 |esf2|SPBC3H7.17c|U3 snoRNP-associated protein Esf2...    25   5.8  
SPAC57A7.10c |sec21||coatomer gamma subunit Sec21 |Schizosacchar...    25   7.6  
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces...    25   7.6  
SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizos...    25   7.6  
SPBC20F10.04c |nse4|rad62|Smc5-6 complex non-SMC subunit Nse4|Sc...    25   7.6  
SPAC1093.03 |||inositol polyphosphate phosphatase |Schizosacchar...    25   7.6  

>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1236

 Score = 34.7 bits (76), Expect = 0.007
 Identities = 26/88 (29%), Positives = 48/88 (54%), Gaps = 2/88 (2%)
 Frame = -2

Query: 263  SAVATGARGGLSTIAGPTEIGTNSST--MAGLTEIGSNSLTMAGPTEMGSNSSMMAGPTA 90
            S++AT +  G ST +  T   T+SST  +A  +  GS++ ++A  +   S++S +A  +A
Sbjct: 976  SSIATASVSGSSTSSVATASATDSSTSSIAAASVTGSSTSSVATASVTDSSTSSVATASA 1035

Query: 89   IGTKSSMMGAGAATPGLTRPLVGPATAT 6
              + +S +   + T G +   V  A+AT
Sbjct: 1036 TDSSTSSIAVASVT-GSSTSSVATASAT 1062



 Score = 31.1 bits (67), Expect = 0.088
 Identities = 26/115 (22%), Positives = 54/115 (46%), Gaps = 2/115 (1%)
 Frame = -2

Query: 341  NGIDTGSGDNRGRASLVDVQNDLHERSAVATGARGGLSTIAGPTEIGTNSST--MAGLTE 168
            + + T S  +   +S+          S+VAT +    ST +  T   T+SST  +A  + 
Sbjct: 989  SSVATASATDSSTSSIAAASVTGSSTSSVATASVTDSSTSSVATASATDSSTSSIAVASV 1048

Query: 167  IGSNSLTMAGPTEMGSNSSMMAGPTAIGTKSSMMGAGAATPGLTRPLVGPATATA 3
             GS++ ++A  +   S++S +A  +  G+ SS +   + T   T  +   ++ ++
Sbjct: 1049 TGSSTSSVATASATDSSTSSVATASITGSLSSSIATASVTGSPTSSVTAVSSTSS 1103


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 31.9 bits (69), Expect = 0.050
 Identities = 20/90 (22%), Positives = 46/90 (51%), Gaps = 3/90 (3%)
 Frame = -2

Query: 263 SAVATGARGGLSTIAGPTEIGTNSSTMAGLTEIGSNSLTMAGPTE---MGSNSSMMAGPT 93
           S+ +T     LST + P+   ++SST + L+   S+S   +  +    + S+SS  + PT
Sbjct: 225 SSSSTLTSSSLSTSSIPSTSSSSSSTSSSLSSSSSSSTASSSSSSSSIISSSSSSSSSPT 284

Query: 92  AIGTKSSMMGAGAATPGLTRPLVGPATATA 3
           +  +  S   + +++P  T   +  +++++
Sbjct: 285 STSSTISSSSSSSSSPTSTSSTISSSSSSS 314


>SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit
           Pst2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1075

 Score = 28.3 bits (60), Expect = 0.62
 Identities = 12/23 (52%), Positives = 14/23 (60%)
 Frame = -2

Query: 413 GSIDNNHGLSYDFGFVNDNHGLW 345
           G ID N  +S  FGF+  N GLW
Sbjct: 287 GIIDRNILVSRGFGFLKSNSGLW 309


>SPAC26A3.15c |nsp1||nucleoporin Nsp1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 598

 Score = 27.9 bits (59), Expect = 0.82
 Identities = 18/74 (24%), Positives = 29/74 (39%)
 Frame = -2

Query: 263 SAVATGARGGLSTIAGPTEIGTNSSTMAGLTEIGSNSLTMAGPTEMGSNSSMMAGPTAIG 84
           +A +TG+  G     G T     +S+ +     GS   +   P  +GS ++     TA  
Sbjct: 165 AAASTGSLFGKPAATGTTSNAPPASSTSTTPATGSGGFSFGKPASLGSTNNASTSTTANS 224

Query: 83  TKSSMMGAGAATPG 42
             S    A  + PG
Sbjct: 225 GFSFGKPATTSAPG 238


>SPAC1B1.01 |||transcription factor Rdp1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 478

 Score = 27.5 bits (58), Expect = 1.1
 Identities = 29/110 (26%), Positives = 42/110 (38%), Gaps = 1/110 (0%)
 Frame = -2

Query: 407 IDNNHGLSYDFGFVN-DNHGLWDNGIDTGSGDNRGRASLVDVQNDLHERSAVATGARGGL 231
           + +N  LS +  ++N D +      +D    +N GR   V+        SAVAT A   L
Sbjct: 15  VTSNDELSSNKIYMNADQNNSRKKEMDGSLQNNGGRLQNVNPLGGNESMSAVATSAASSL 74

Query: 230 STIAGPTEIGTNSSTMAGLTEIGSNSLTMAGPTEMGSNSSMMAGPTAIGT 81
            T      +   S T+   T    +   M+G    G  S    G T  GT
Sbjct: 75  PTAENGVSLNAASPTIHSNTPTVVSHPVMSGSELKGEESHNSPG-TLNGT 123


>SPCC553.01c ||SPCC736.01c|meiotic chromosome segregation
           protein|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 715

 Score = 26.6 bits (56), Expect = 1.9
 Identities = 24/111 (21%), Positives = 44/111 (39%), Gaps = 2/111 (1%)
 Frame = -2

Query: 329 TGSGDNRGRASLVDVQNDLHERSAVATGARGGLSTIAGPT--EIGTNSSTMAGLTEIGSN 156
           TG   N    ++ D   +L+ERS         L  +   T  EI  +   +   +E   +
Sbjct: 363 TGKRQNFINITVTDPSKNLYERSVKTAAELASLQNVTETTDNEIPNSPDFLDAASEEDEH 422

Query: 155 SLTMAGPTEMGSNSSMMAGPTAIGTKSSMMGAGAATPGLTRPLVGPATATA 3
             ++        +SS +  P A+  K +        P  + PLV PA++++
Sbjct: 423 IPSL--------DSSSLLTPLALPNKKAFQPVKFRVPSFSSPLVKPASSSS 465


>SPBC354.10 |||RNAPII degradation factor |Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 963

 Score = 26.6 bits (56), Expect = 1.9
 Identities = 22/78 (28%), Positives = 32/78 (41%), Gaps = 2/78 (2%)
 Frame = -2

Query: 248  GARGGLSTIAGPTEIGTNSSTMAGLTE--IGSNSLTMAGPTEMGSNSSMMAGPTAIGTKS 75
            G   G+S       I   ++  +G+TE   G     +      G+NS      + +G  S
Sbjct: 804  GNVSGMSGTQTSNPINNATANTSGMTEKAAGHRDSVIGNGGVTGANSMS----STLGGLS 859

Query: 74   SMMGAGAATPGLTRPLVG 21
            SM G G A PG+    VG
Sbjct: 860  SMSGMGRAAPGMFMGNVG 877


>SPBC4F6.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 674

 Score = 26.2 bits (55), Expect = 2.5
 Identities = 14/36 (38%), Positives = 22/36 (61%)
 Frame = -2

Query: 449 GSNVSNLDRLGDGSIDNNHGLSYDFGFVNDNHGLWD 342
           GSNV +++  GD S D N  ++++   + DN GL D
Sbjct: 246 GSNVDDVEDSGDSSSDKN-SINHE---IRDNEGLED 277


>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 857

 Score = 26.2 bits (55), Expect = 2.5
 Identities = 24/80 (30%), Positives = 36/80 (45%), Gaps = 6/80 (7%)
 Frame = +1

Query: 34  LVSPGVAAP--APIIDDFVPIAVGPA--IIDEFEPISVGPA--IVNEFEPISVRPAIVDE 195
           +++P V  P  AP++ +   +   PA  +  E       PA  +V E   +  RPA    
Sbjct: 509 VITPSVPQPPAAPVVPEAPSVHQPPAAPVAPEVPSAPQRPAAPVVPEAPSVPQRPA---- 564

Query: 196 FVPISVGPAIVDNPPLAPVA 255
            VP+      V  PP+APVA
Sbjct: 565 -VPVVPEALSVPQPPVAPVA 583


>SPAC2G11.12 |rqh1|hus2, rad12, rec9|RecQ type DNA helicase
            Rqh1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1328

 Score = 26.2 bits (55), Expect = 2.5
 Identities = 15/59 (25%), Positives = 27/59 (45%), Gaps = 1/59 (1%)
 Frame = -2

Query: 416  DGSIDNNH-GLSYDFGFVNDNHGLWDNGIDTGSGDNRGRASLVDVQNDLHERSAVATGA 243
            D  ID N   L ++ GF +D+  +++       GD        D+ N ++ +S   TG+
Sbjct: 1211 DYPIDTNALSLDHEQGFSDDSDSVYEPSSPIEEGDEEVDGQRKDILNFMNSQSLTQTGS 1269


>SPAC29A4.08c |prp19|cwf8|ubiquitin-protein ligase E4
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 488

 Score = 25.8 bits (54), Expect = 3.3
 Identities = 11/37 (29%), Positives = 20/37 (54%)
 Frame = +1

Query: 160 EPISVRPAIVDEFVPISVGPAIVDNPPLAPVATALLS 270
           +P++ +   +++ VP+ V   +   PP A    ALLS
Sbjct: 38  DPVTQQECTLEDLVPVKVPDFVRPRPPSATSLPALLS 74


>SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 830

 Score = 25.4 bits (53), Expect = 4.4
 Identities = 22/85 (25%), Positives = 35/85 (41%)
 Frame = -2

Query: 317 DNRGRASLVDVQNDLHERSAVATGARGGLSTIAGPTEIGTNSSTMAGLTEIGSNSLTMAG 138
           DN   ++L    N   + S+  + +    S  A PT   + +ST   LT   S SL    
Sbjct: 155 DNEYHSNLATSSNPFSDGSSSKSASLTASSAAASPTVSFSPASTSENLTPTSSKSL---- 210

Query: 137 PTEMGSNSSMMAGPTAIGTKSSMMG 63
                SN+S++    +    SS+ G
Sbjct: 211 ----ASNTSLVQSFNSASRSSSISG 231


>SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1274

 Score = 25.4 bits (53), Expect = 4.4
 Identities = 16/37 (43%), Positives = 19/37 (51%), Gaps = 2/37 (5%)
 Frame = -1

Query: 246 GKRRVVYDS--GSNGDRYELVDDGRPNGDRFEFVDNG 142
           GKR VV  S  G+NG  Y  V  G P G  F+ +  G
Sbjct: 413 GKRNVVIVSSAGNNGPAYTTV--GAPGGTTFDVISVG 447


>SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 751

 Score = 25.0 bits (52), Expect = 5.8
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = -2

Query: 215 PTEIGTNSSTMAGLTEIGSNSLTMAGPTEMGSNSS 111
           PTE   N +T+  LT IGS  + M G +   SN S
Sbjct: 335 PTEGSANLNTVTELTGIGSR-IAMNGSSVKASNIS 368


>SPAC13C5.02 |dre4||DNA replication protein Dre4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 411

 Score = 25.0 bits (52), Expect = 5.8
 Identities = 10/21 (47%), Positives = 13/21 (61%)
 Frame = -1

Query: 120 ELIYDGRSDSDRYKVVDDGSR 58
           EL+Y    D DR+ V+D G R
Sbjct: 262 ELVYPKLLDDDRFYVLDSGER 282


>SPBC28E12.05 |esf2|SPBC3H7.17c|U3 snoRNP-associated protein Esf2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 334

 Score = 25.0 bits (52), Expect = 5.8
 Identities = 9/18 (50%), Positives = 13/18 (72%)
 Frame = -1

Query: 270 REECCRNGGKRRVVYDSG 217
           R +  RNGG +RV+Y+ G
Sbjct: 161 RAQRLRNGGNKRVMYEEG 178


>SPAC57A7.10c |sec21||coatomer gamma subunit Sec21
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 905

 Score = 24.6 bits (51), Expect = 7.6
 Identities = 12/34 (35%), Positives = 16/34 (47%)
 Frame = +1

Query: 34  LVSPGVAAPAPIIDDFVPIAVGPAIIDEFEPISV 135
           ++   V    P  DD V   V PA I   EP+S+
Sbjct: 705 ILENAVVVSTPSTDDLVEECVVPAAIVSGEPVSI 738


>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1485

 Score = 24.6 bits (51), Expect = 7.6
 Identities = 15/42 (35%), Positives = 26/42 (61%)
 Frame = -2

Query: 404  DNNHGLSYDFGFVNDNHGLWDNGIDTGSGDNRGRASLVDVQN 279
            DN+HGLS +   ++ + GL D+   + SG +R R+  VD ++
Sbjct: 1295 DNSHGLSVEENRISKSPGL-DS---SDSGKSRKRSQSVDSED 1332


>SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 855

 Score = 24.6 bits (51), Expect = 7.6
 Identities = 23/88 (26%), Positives = 37/88 (42%)
 Frame = -2

Query: 299 SLVDVQNDLHERSAVATGARGGLSTIAGPTEIGTNSSTMAGLTEIGSNSLTMAGPTEMGS 120
           ++ DV  + HE S+    + G  S ++ P+ I   SS         SNS     P    +
Sbjct: 371 NIFDVSRNNHEVSSPLIQSPG--SYVSMPS-INMVSSLPISAPVPNSNSQFPRRPNTFRT 427

Query: 119 NSSMMAGPTAIGTKSSMMGAGAATPGLT 36
           NSS   G  + G  S+   A +  P ++
Sbjct: 428 NSSKSVGQGSSGVDSNQENAESFNPSIS 455


>SPBC20F10.04c |nse4|rad62|Smc5-6 complex non-SMC subunit
           Nse4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 300

 Score = 24.6 bits (51), Expect = 7.6
 Identities = 13/49 (26%), Positives = 22/49 (44%)
 Frame = -2

Query: 323 SGDNRGRASLVDVQNDLHERSAVATGARGGLSTIAGPTEIGTNSSTMAG 177
           +G+ R    ++D+  DL+E        +  +     P E  TNS+T  G
Sbjct: 252 AGEIRNIQLVLDMTMDLYENIIKEYNIKESIIPTRAPVETSTNSNTWYG 300


>SPAC1093.03 |||inositol polyphosphate phosphatase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 832

 Score = 24.6 bits (51), Expect = 7.6
 Identities = 18/70 (25%), Positives = 32/70 (45%), Gaps = 2/70 (2%)
 Frame = +1

Query: 46  GVAAPAPIIDDFVPIAVGPAIIDEFEPISVGPAIVN--EFEPISVRPAIVDEFVPISVGP 219
           G+  PA   D+       P  I  F  + + P +++   F P+S+ P +   F+P+S   
Sbjct: 600 GIKFPANYFDNVYT----PNSISSFSEVLL-PNLISTLNFAPLSLIPLLRKSFLPLSYNG 654

Query: 220 AIVDNPPLAP 249
             ++ P L P
Sbjct: 655 FGIEAPSLNP 664


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,481,956
Number of Sequences: 5004
Number of extensions: 28655
Number of successful extensions: 110
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 92
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 109
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 178394480
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -