BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0514 (468 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 35 0.007 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 32 0.050 SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit Pst... 28 0.62 SPAC26A3.15c |nsp1||nucleoporin Nsp1|Schizosaccharomyces pombe|c... 28 0.82 SPAC1B1.01 |||transcription factor Rdp1|Schizosaccharomyces pomb... 27 1.1 SPCC553.01c ||SPCC736.01c|meiotic chromosome segregation protein... 27 1.9 SPBC354.10 |||RNAPII degradation factor |Schizosaccharomyces pom... 27 1.9 SPBC4F6.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 26 2.5 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 26 2.5 SPAC2G11.12 |rqh1|hus2, rad12, rec9|RecQ type DNA helicase Rqh1|... 26 2.5 SPAC29A4.08c |prp19|cwf8|ubiquitin-protein ligase E4 |Schizosacc... 26 3.3 SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe... 25 4.4 SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|c... 25 4.4 SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 5.8 SPAC13C5.02 |dre4||DNA replication protein Dre4|Schizosaccharomy... 25 5.8 SPBC28E12.05 |esf2|SPBC3H7.17c|U3 snoRNP-associated protein Esf2... 25 5.8 SPAC57A7.10c |sec21||coatomer gamma subunit Sec21 |Schizosacchar... 25 7.6 SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 25 7.6 SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizos... 25 7.6 SPBC20F10.04c |nse4|rad62|Smc5-6 complex non-SMC subunit Nse4|Sc... 25 7.6 SPAC1093.03 |||inositol polyphosphate phosphatase |Schizosacchar... 25 7.6 >SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1236 Score = 34.7 bits (76), Expect = 0.007 Identities = 26/88 (29%), Positives = 48/88 (54%), Gaps = 2/88 (2%) Frame = -2 Query: 263 SAVATGARGGLSTIAGPTEIGTNSST--MAGLTEIGSNSLTMAGPTEMGSNSSMMAGPTA 90 S++AT + G ST + T T+SST +A + GS++ ++A + S++S +A +A Sbjct: 976 SSIATASVSGSSTSSVATASATDSSTSSIAAASVTGSSTSSVATASVTDSSTSSVATASA 1035 Query: 89 IGTKSSMMGAGAATPGLTRPLVGPATAT 6 + +S + + T G + V A+AT Sbjct: 1036 TDSSTSSIAVASVT-GSSTSSVATASAT 1062 Score = 31.1 bits (67), Expect = 0.088 Identities = 26/115 (22%), Positives = 54/115 (46%), Gaps = 2/115 (1%) Frame = -2 Query: 341 NGIDTGSGDNRGRASLVDVQNDLHERSAVATGARGGLSTIAGPTEIGTNSST--MAGLTE 168 + + T S + +S+ S+VAT + ST + T T+SST +A + Sbjct: 989 SSVATASATDSSTSSIAAASVTGSSTSSVATASVTDSSTSSVATASATDSSTSSIAVASV 1048 Query: 167 IGSNSLTMAGPTEMGSNSSMMAGPTAIGTKSSMMGAGAATPGLTRPLVGPATATA 3 GS++ ++A + S++S +A + G+ SS + + T T + ++ ++ Sbjct: 1049 TGSSTSSVATASATDSSTSSVATASITGSLSSSIATASVTGSPTSSVTAVSSTSS 1103 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 31.9 bits (69), Expect = 0.050 Identities = 20/90 (22%), Positives = 46/90 (51%), Gaps = 3/90 (3%) Frame = -2 Query: 263 SAVATGARGGLSTIAGPTEIGTNSSTMAGLTEIGSNSLTMAGPTE---MGSNSSMMAGPT 93 S+ +T LST + P+ ++SST + L+ S+S + + + S+SS + PT Sbjct: 225 SSSSTLTSSSLSTSSIPSTSSSSSSTSSSLSSSSSSSTASSSSSSSSIISSSSSSSSSPT 284 Query: 92 AIGTKSSMMGAGAATPGLTRPLVGPATATA 3 + + S + +++P T + +++++ Sbjct: 285 STSSTISSSSSSSSSPTSTSSTISSSSSSS 314 >SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit Pst2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1075 Score = 28.3 bits (60), Expect = 0.62 Identities = 12/23 (52%), Positives = 14/23 (60%) Frame = -2 Query: 413 GSIDNNHGLSYDFGFVNDNHGLW 345 G ID N +S FGF+ N GLW Sbjct: 287 GIIDRNILVSRGFGFLKSNSGLW 309 >SPAC26A3.15c |nsp1||nucleoporin Nsp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 598 Score = 27.9 bits (59), Expect = 0.82 Identities = 18/74 (24%), Positives = 29/74 (39%) Frame = -2 Query: 263 SAVATGARGGLSTIAGPTEIGTNSSTMAGLTEIGSNSLTMAGPTEMGSNSSMMAGPTAIG 84 +A +TG+ G G T +S+ + GS + P +GS ++ TA Sbjct: 165 AAASTGSLFGKPAATGTTSNAPPASSTSTTPATGSGGFSFGKPASLGSTNNASTSTTANS 224 Query: 83 TKSSMMGAGAATPG 42 S A + PG Sbjct: 225 GFSFGKPATTSAPG 238 >SPAC1B1.01 |||transcription factor Rdp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 478 Score = 27.5 bits (58), Expect = 1.1 Identities = 29/110 (26%), Positives = 42/110 (38%), Gaps = 1/110 (0%) Frame = -2 Query: 407 IDNNHGLSYDFGFVN-DNHGLWDNGIDTGSGDNRGRASLVDVQNDLHERSAVATGARGGL 231 + +N LS + ++N D + +D +N GR V+ SAVAT A L Sbjct: 15 VTSNDELSSNKIYMNADQNNSRKKEMDGSLQNNGGRLQNVNPLGGNESMSAVATSAASSL 74 Query: 230 STIAGPTEIGTNSSTMAGLTEIGSNSLTMAGPTEMGSNSSMMAGPTAIGT 81 T + S T+ T + M+G G S G T GT Sbjct: 75 PTAENGVSLNAASPTIHSNTPTVVSHPVMSGSELKGEESHNSPG-TLNGT 123 >SPCC553.01c ||SPCC736.01c|meiotic chromosome segregation protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 715 Score = 26.6 bits (56), Expect = 1.9 Identities = 24/111 (21%), Positives = 44/111 (39%), Gaps = 2/111 (1%) Frame = -2 Query: 329 TGSGDNRGRASLVDVQNDLHERSAVATGARGGLSTIAGPT--EIGTNSSTMAGLTEIGSN 156 TG N ++ D +L+ERS L + T EI + + +E + Sbjct: 363 TGKRQNFINITVTDPSKNLYERSVKTAAELASLQNVTETTDNEIPNSPDFLDAASEEDEH 422 Query: 155 SLTMAGPTEMGSNSSMMAGPTAIGTKSSMMGAGAATPGLTRPLVGPATATA 3 ++ +SS + P A+ K + P + PLV PA++++ Sbjct: 423 IPSL--------DSSSLLTPLALPNKKAFQPVKFRVPSFSSPLVKPASSSS 465 >SPBC354.10 |||RNAPII degradation factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 963 Score = 26.6 bits (56), Expect = 1.9 Identities = 22/78 (28%), Positives = 32/78 (41%), Gaps = 2/78 (2%) Frame = -2 Query: 248 GARGGLSTIAGPTEIGTNSSTMAGLTE--IGSNSLTMAGPTEMGSNSSMMAGPTAIGTKS 75 G G+S I ++ +G+TE G + G+NS + +G S Sbjct: 804 GNVSGMSGTQTSNPINNATANTSGMTEKAAGHRDSVIGNGGVTGANSMS----STLGGLS 859 Query: 74 SMMGAGAATPGLTRPLVG 21 SM G G A PG+ VG Sbjct: 860 SMSGMGRAAPGMFMGNVG 877 >SPBC4F6.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 674 Score = 26.2 bits (55), Expect = 2.5 Identities = 14/36 (38%), Positives = 22/36 (61%) Frame = -2 Query: 449 GSNVSNLDRLGDGSIDNNHGLSYDFGFVNDNHGLWD 342 GSNV +++ GD S D N ++++ + DN GL D Sbjct: 246 GSNVDDVEDSGDSSSDKN-SINHE---IRDNEGLED 277 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 26.2 bits (55), Expect = 2.5 Identities = 24/80 (30%), Positives = 36/80 (45%), Gaps = 6/80 (7%) Frame = +1 Query: 34 LVSPGVAAP--APIIDDFVPIAVGPA--IIDEFEPISVGPA--IVNEFEPISVRPAIVDE 195 +++P V P AP++ + + PA + E PA +V E + RPA Sbjct: 509 VITPSVPQPPAAPVVPEAPSVHQPPAAPVAPEVPSAPQRPAAPVVPEAPSVPQRPA---- 564 Query: 196 FVPISVGPAIVDNPPLAPVA 255 VP+ V PP+APVA Sbjct: 565 -VPVVPEALSVPQPPVAPVA 583 >SPAC2G11.12 |rqh1|hus2, rad12, rec9|RecQ type DNA helicase Rqh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1328 Score = 26.2 bits (55), Expect = 2.5 Identities = 15/59 (25%), Positives = 27/59 (45%), Gaps = 1/59 (1%) Frame = -2 Query: 416 DGSIDNNH-GLSYDFGFVNDNHGLWDNGIDTGSGDNRGRASLVDVQNDLHERSAVATGA 243 D ID N L ++ GF +D+ +++ GD D+ N ++ +S TG+ Sbjct: 1211 DYPIDTNALSLDHEQGFSDDSDSVYEPSSPIEEGDEEVDGQRKDILNFMNSQSLTQTGS 1269 >SPAC29A4.08c |prp19|cwf8|ubiquitin-protein ligase E4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 488 Score = 25.8 bits (54), Expect = 3.3 Identities = 11/37 (29%), Positives = 20/37 (54%) Frame = +1 Query: 160 EPISVRPAIVDEFVPISVGPAIVDNPPLAPVATALLS 270 +P++ + +++ VP+ V + PP A ALLS Sbjct: 38 DPVTQQECTLEDLVPVKVPDFVRPRPPSATSLPALLS 74 >SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 830 Score = 25.4 bits (53), Expect = 4.4 Identities = 22/85 (25%), Positives = 35/85 (41%) Frame = -2 Query: 317 DNRGRASLVDVQNDLHERSAVATGARGGLSTIAGPTEIGTNSSTMAGLTEIGSNSLTMAG 138 DN ++L N + S+ + + S A PT + +ST LT S SL Sbjct: 155 DNEYHSNLATSSNPFSDGSSSKSASLTASSAAASPTVSFSPASTSENLTPTSSKSL---- 210 Query: 137 PTEMGSNSSMMAGPTAIGTKSSMMG 63 SN+S++ + SS+ G Sbjct: 211 ----ASNTSLVQSFNSASRSSSISG 231 >SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1274 Score = 25.4 bits (53), Expect = 4.4 Identities = 16/37 (43%), Positives = 19/37 (51%), Gaps = 2/37 (5%) Frame = -1 Query: 246 GKRRVVYDS--GSNGDRYELVDDGRPNGDRFEFVDNG 142 GKR VV S G+NG Y V G P G F+ + G Sbjct: 413 GKRNVVIVSSAGNNGPAYTTV--GAPGGTTFDVISVG 447 >SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 751 Score = 25.0 bits (52), Expect = 5.8 Identities = 15/35 (42%), Positives = 19/35 (54%) Frame = -2 Query: 215 PTEIGTNSSTMAGLTEIGSNSLTMAGPTEMGSNSS 111 PTE N +T+ LT IGS + M G + SN S Sbjct: 335 PTEGSANLNTVTELTGIGSR-IAMNGSSVKASNIS 368 >SPAC13C5.02 |dre4||DNA replication protein Dre4|Schizosaccharomyces pombe|chr 1|||Manual Length = 411 Score = 25.0 bits (52), Expect = 5.8 Identities = 10/21 (47%), Positives = 13/21 (61%) Frame = -1 Query: 120 ELIYDGRSDSDRYKVVDDGSR 58 EL+Y D DR+ V+D G R Sbjct: 262 ELVYPKLLDDDRFYVLDSGER 282 >SPBC28E12.05 |esf2|SPBC3H7.17c|U3 snoRNP-associated protein Esf2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 334 Score = 25.0 bits (52), Expect = 5.8 Identities = 9/18 (50%), Positives = 13/18 (72%) Frame = -1 Query: 270 REECCRNGGKRRVVYDSG 217 R + RNGG +RV+Y+ G Sbjct: 161 RAQRLRNGGNKRVMYEEG 178 >SPAC57A7.10c |sec21||coatomer gamma subunit Sec21 |Schizosaccharomyces pombe|chr 1|||Manual Length = 905 Score = 24.6 bits (51), Expect = 7.6 Identities = 12/34 (35%), Positives = 16/34 (47%) Frame = +1 Query: 34 LVSPGVAAPAPIIDDFVPIAVGPAIIDEFEPISV 135 ++ V P DD V V PA I EP+S+ Sbjct: 705 ILENAVVVSTPSTDDLVEECVVPAAIVSGEPVSI 738 >SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces pombe|chr 2|||Manual Length = 1485 Score = 24.6 bits (51), Expect = 7.6 Identities = 15/42 (35%), Positives = 26/42 (61%) Frame = -2 Query: 404 DNNHGLSYDFGFVNDNHGLWDNGIDTGSGDNRGRASLVDVQN 279 DN+HGLS + ++ + GL D+ + SG +R R+ VD ++ Sbjct: 1295 DNSHGLSVEENRISKSPGL-DS---SDSGKSRKRSQSVDSED 1332 >SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 855 Score = 24.6 bits (51), Expect = 7.6 Identities = 23/88 (26%), Positives = 37/88 (42%) Frame = -2 Query: 299 SLVDVQNDLHERSAVATGARGGLSTIAGPTEIGTNSSTMAGLTEIGSNSLTMAGPTEMGS 120 ++ DV + HE S+ + G S ++ P+ I SS SNS P + Sbjct: 371 NIFDVSRNNHEVSSPLIQSPG--SYVSMPS-INMVSSLPISAPVPNSNSQFPRRPNTFRT 427 Query: 119 NSSMMAGPTAIGTKSSMMGAGAATPGLT 36 NSS G + G S+ A + P ++ Sbjct: 428 NSSKSVGQGSSGVDSNQENAESFNPSIS 455 >SPBC20F10.04c |nse4|rad62|Smc5-6 complex non-SMC subunit Nse4|Schizosaccharomyces pombe|chr 2|||Manual Length = 300 Score = 24.6 bits (51), Expect = 7.6 Identities = 13/49 (26%), Positives = 22/49 (44%) Frame = -2 Query: 323 SGDNRGRASLVDVQNDLHERSAVATGARGGLSTIAGPTEIGTNSSTMAG 177 +G+ R ++D+ DL+E + + P E TNS+T G Sbjct: 252 AGEIRNIQLVLDMTMDLYENIIKEYNIKESIIPTRAPVETSTNSNTWYG 300 >SPAC1093.03 |||inositol polyphosphate phosphatase |Schizosaccharomyces pombe|chr 1|||Manual Length = 832 Score = 24.6 bits (51), Expect = 7.6 Identities = 18/70 (25%), Positives = 32/70 (45%), Gaps = 2/70 (2%) Frame = +1 Query: 46 GVAAPAPIIDDFVPIAVGPAIIDEFEPISVGPAIVN--EFEPISVRPAIVDEFVPISVGP 219 G+ PA D+ P I F + + P +++ F P+S+ P + F+P+S Sbjct: 600 GIKFPANYFDNVYT----PNSISSFSEVLL-PNLISTLNFAPLSLIPLLRKSFLPLSYNG 654 Query: 220 AIVDNPPLAP 249 ++ P L P Sbjct: 655 FGIEAPSLNP 664 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,481,956 Number of Sequences: 5004 Number of extensions: 28655 Number of successful extensions: 110 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 92 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 109 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 178394480 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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