BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0503 (657 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23A1.11 |rpl1602|rpl16-2|60S ribosomal protein L13/L16|Schiz... 120 1e-28 SPBC839.13c |rpl1601||60S ribosomal protein L13/L16|Schizosaccha... 120 1e-28 SPBC2G2.05 |rpl1603|rpl16c|60S ribosomal protein L13/L16|Schizos... 118 1e-27 SPBP8B7.17c |||phosphomethylpyrimidine kinase|Schizosaccharomyce... 29 0.78 SPAC1B9.02c |sck1||serine/threonine protein kinase Sck1|Schizosa... 26 4.2 SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-pho... 26 5.5 SPCC1020.08 |||wybutosine biosynthesis protein Tyw1|Schizosaccha... 25 7.3 SPBC13E7.06 |msd1|mug172|spindle pole body protein Msd1|Schizosa... 25 7.3 >SPAC23A1.11 |rpl1602|rpl16-2|60S ribosomal protein L13/L16|Schizosaccharomyces pombe|chr 1|||Manual Length = 197 Score = 120 bits (290), Expect = 1e-28 Identities = 53/75 (70%), Positives = 65/75 (86%) Frame = +3 Query: 24 KAIVIDGRGHLLGRLAAVIAKVLLEGNKVVVVRCEQINISGNFFRNKLKLMSFLRKRCNV 203 K +VID +GHLLGRLA+V+AK LL G KVVVVRCE++NISG+FFRNKLK +++LRK C Sbjct: 6 KVVVIDAKGHLLGRLASVVAKQLLGGQKVVVVRCEELNISGHFFRNKLKYLAYLRKACRY 65 Query: 204 NPARGPFHFRAPSKI 248 NP+RG FHFRAPS+I Sbjct: 66 NPSRGAFHFRAPSRI 80 Score = 99.1 bits (236), Expect = 5e-22 Identities = 44/83 (53%), Positives = 54/83 (65%) Frame = +2 Query: 254 ETVRGMIPHKTERGKNALRRLRTYDGCPPPFDNXXXXXXXXXXXXFCLKPGRNYCHVGRL 433 + VRGM+PHKT RG+ AL L+ +G PPPFD LKPGR YC VGRL Sbjct: 83 KAVRGMLPHKTARGQAALEHLQAVEGIPPPFDKQKRVVVPAALRVLRLKPGRKYCTVGRL 142 Query: 434 SHEIGWKYRDVVRKLEDKRKGKA 502 S E+GWKY D+V KLE++RK K+ Sbjct: 143 SSEVGWKYSDIVSKLEERRKVKS 165 >SPBC839.13c |rpl1601||60S ribosomal protein L13/L16|Schizosaccharomyces pombe|chr 2|||Manual Length = 197 Score = 120 bits (290), Expect = 1e-28 Identities = 53/75 (70%), Positives = 65/75 (86%) Frame = +3 Query: 24 KAIVIDGRGHLLGRLAAVIAKVLLEGNKVVVVRCEQINISGNFFRNKLKLMSFLRKRCNV 203 K +VID +GHLLGRLA+V+AK LL G KVVVVRCE++NISG+FFRNKLK +++LRK C Sbjct: 6 KVVVIDAKGHLLGRLASVVAKQLLGGQKVVVVRCEELNISGHFFRNKLKYLAYLRKACRY 65 Query: 204 NPARGPFHFRAPSKI 248 NP+RG FHFRAPS+I Sbjct: 66 NPSRGAFHFRAPSRI 80 Score = 99.5 bits (237), Expect = 4e-22 Identities = 44/83 (53%), Positives = 54/83 (65%) Frame = +2 Query: 254 ETVRGMIPHKTERGKNALRRLRTYDGCPPPFDNXXXXXXXXXXXXFCLKPGRNYCHVGRL 433 + VRGM+PHKT RG+ AL L+ +G PPPFD LKPGR YC VGRL Sbjct: 83 KAVRGMLPHKTARGQAALEHLQAVEGIPPPFDKQKRVVVPAALRVLRLKPGRKYCTVGRL 142 Query: 434 SHEIGWKYRDVVRKLEDKRKGKA 502 S E+GWKY D+V KLE++RK K+ Sbjct: 143 SSEVGWKYNDIVAKLEERRKVKS 165 >SPBC2G2.05 |rpl1603|rpl16c|60S ribosomal protein L13/L16|Schizosaccharomyces pombe|chr 2|||Manual Length = 197 Score = 118 bits (283), Expect = 1e-27 Identities = 50/75 (66%), Positives = 64/75 (85%) Frame = +3 Query: 24 KAIVIDGRGHLLGRLAAVIAKVLLEGNKVVVVRCEQINISGNFFRNKLKLMSFLRKRCNV 203 K ++ID +GHL+GRLA+ +AK LL G KVVVVRCE++NISG+FFRNKLK +++LRK C Sbjct: 6 KLVIIDAKGHLMGRLASTVAKQLLAGQKVVVVRCEELNISGHFFRNKLKYLAYLRKACRY 65 Query: 204 NPARGPFHFRAPSKI 248 NP+RG FHFRAPS+I Sbjct: 66 NPSRGAFHFRAPSRI 80 Score = 95.1 bits (226), Expect = 8e-21 Identities = 41/83 (49%), Positives = 54/83 (65%) Frame = +2 Query: 254 ETVRGMIPHKTERGKNALRRLRTYDGCPPPFDNXXXXXXXXXXXXFCLKPGRNYCHVGRL 433 + VRGM+PHKT RG AL+ L+ +G PPPFD LKP R YC +GRL Sbjct: 83 KAVRGMLPHKTTRGNIALKNLQALEGIPPPFDKQKRLVVPAALRVLRLKPSRKYCTIGRL 142 Query: 434 SHEIGWKYRDVVRKLEDKRKGKA 502 S E+GWKY+++V KLE++RK K+ Sbjct: 143 SSEVGWKYKNIVSKLEERRKIKS 165 >SPBP8B7.17c |||phosphomethylpyrimidine kinase|Schizosaccharomyces pombe|chr 2|||Manual Length = 506 Score = 28.7 bits (61), Expect = 0.78 Identities = 14/35 (40%), Positives = 21/35 (60%) Frame = +3 Query: 51 HLLGRLAAVIAKVLLEGNKVVVVRCEQINISGNFF 155 H L + AV K++ G K VV+RC+ I + +FF Sbjct: 157 HHLEDVKAVGKKLIKAGCKNVVIRCDDIPFASDFF 191 >SPAC1B9.02c |sck1||serine/threonine protein kinase Sck1|Schizosaccharomyces pombe|chr 1|||Manual Length = 696 Score = 26.2 bits (55), Expect = 4.2 Identities = 15/55 (27%), Positives = 27/55 (49%) Frame = -3 Query: 235 ALKWKGPRAGFTLHLLRRNDISLSLFLKKLPEMLICSQRTTTTLFPSRRTLAMTA 71 AL+ G ++L+R+ND + +KK+ + LI ++ T R L T+ Sbjct: 304 ALRLIGKGTFGQVYLVRKNDTNRIYAMKKISKKLIVRKKEVTHTLGERNILVRTS 358 >SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-phosphate 5-kinase Fab1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1932 Score = 25.8 bits (54), Expect = 5.5 Identities = 10/24 (41%), Positives = 17/24 (70%) Frame = -1 Query: 336 GHPSYVRSLLRAFLPRSVLCGIMP 265 G+ SY+ + L+AFLP++V + P Sbjct: 381 GNESYLSNFLKAFLPKTVCDYLFP 404 >SPCC1020.08 |||wybutosine biosynthesis protein Tyw1|Schizosaccharomyces pombe|chr 3|||Manual Length = 688 Score = 25.4 bits (53), Expect = 7.3 Identities = 11/35 (31%), Positives = 20/35 (57%) Frame = -1 Query: 600 LDDGSEWCCRLRHLLTSILGDPLKFLFIGNSLTAL 496 L D E R+RH S++G+P+ + +I ++ L Sbjct: 408 LPDRYEEASRVRHCALSLVGEPIFYPYINEFVSML 442 >SPBC13E7.06 |msd1|mug172|spindle pole body protein Msd1|Schizosaccharomyces pombe|chr 2|||Manual Length = 331 Score = 25.4 bits (53), Expect = 7.3 Identities = 16/42 (38%), Positives = 20/42 (47%) Frame = -2 Query: 134 DLFAANHNNFVPFEKDLGDDCRQTAQQMTTAIDHYGLIAKAR 9 D+ A N + L RQ QQM T +YGL+ KAR Sbjct: 126 DVNAKNELTLKALKNQLSVSLRQHEQQMETLKGNYGLV-KAR 166 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,804,382 Number of Sequences: 5004 Number of extensions: 57268 Number of successful extensions: 155 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 148 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 155 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 297805304 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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