Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0494
(722 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1683.07 |mal1||alpha-glucosidase Mal1 |Schizosaccharomyces p... 87 2e-18
SPAC23D3.14c |aah2||alpha-amylase homolog Aah2|Schizosaccharomyc... 45 1e-05
SPCC11E10.09c ||SPCC188.01c|alpha-amylase homolog |Schizosacchar... 37 0.003
SPAC27E2.01 |||alpha-amylase homolog |Schizosaccharomyces pombe|... 36 0.008
SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 33 0.055
SPCC63.02c |aah3||alpha-amylase homolog Aah3|Schizosaccharomyces... 33 0.055
SPAC458.06 |||phosphoinositide binding protein|Schizosaccharomyc... 29 0.51
SPBC16A3.13 |meu7|aah4|alpha-amylase homolog Aah4|Schizosaccharo... 29 0.89
SPCC757.12 |||alpha-amylase homolog |Schizosaccharomyces pombe|c... 28 1.2
SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor, ... 28 1.6
SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|ch... 27 2.1
SPCC16A11.17 |cdc21|mcm4, SPCC24B10.01|MCM complex subunit Cdc21... 27 2.1
SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog Mde5|S... 27 2.7
SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosacch... 27 3.6
SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-gluca... 27 3.6
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 26 4.7
SPCC1795.08c |||histone acetyltransferase complex subunit |Schiz... 26 4.7
SPAC19A8.11c |||recombination protein Irc6 |Schizosaccharomyces ... 26 6.3
SPCC18B5.03 |wee1||dual specificity protein kinase Wee1|Schizosa... 26 6.3
SPCC1795.11 |sum3|ded1, slh3, moc2|ATP-dependent RNA helicase Su... 25 8.3
>SPBC1683.07 |mal1||alpha-glucosidase Mal1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 579
Score = 87.4 bits (207), Expect = 2e-18
Identities = 35/70 (50%), Positives = 49/70 (70%)
Frame = +2
Query: 503 HRENGNRQPPNNWLSHFRGSAWEYKEEVGKYYLHQFAVGQPDLNYRNQDVVDEMKNIIRF 682
+ E G R PPNNW S+F SAWE+ E +YYLH ++VGQPDLN+ V + + +I+RF
Sbjct: 141 YNEKGERLPPNNWRSYFDTSAWEWDEATQEYYLHLWSVGQPDLNWETPKVREAVHDILRF 200
Query: 683 WLGKGIAGFQ 712
WL +G+ GF+
Sbjct: 201 WLDRGVDGFR 210
Score = 86.6 bits (205), Expect = 3e-18
Identities = 39/95 (41%), Positives = 62/95 (65%), Gaps = 2/95 (2%)
Frame = +3
Query: 216 KIGIYQRIRCRSCWLSPIFKSPMVDFGYDIANFYEIHHEYGTMEDFEALLKKANELDIKV 395
K+ + + S WL PI+ SP+ D GYD++++ +I YGT+ED + L+K +E D+K+
Sbjct: 44 KVDYLKALNVESIWLCPIYPSPLKDMGYDVSDYKQIDSRYGTLEDLDRLMKALHERDMKL 103
Query: 396 VLDLVPNHTSNESVWFQEALNG--NEKYYNYFVWE 494
V+DLV NHTS++ WF+E+ + N K YF W+
Sbjct: 104 VMDLVLNHTSDQHEWFKESRSSKTNPKRDWYF-WK 137
Score = 64.1 bits (149), Expect = 2e-11
Identities = 25/45 (55%), Positives = 34/45 (75%)
Frame = +1
Query: 121 DWWETSILYQIYPRSFADSDGDGIGDLNGITSKLEYIKELGVGAV 255
+WW + +YQIYP SF DS+GDG GDL GI SK++Y+K L V ++
Sbjct: 12 NWWRETSVYQIYPASFKDSNGDGFGDLEGIISKVDYLKALNVESI 56
>SPAC23D3.14c |aah2||alpha-amylase homolog Aah2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 581
Score = 45.2 bits (102), Expect = 1e-05
Identities = 27/93 (29%), Positives = 52/93 (55%), Gaps = 12/93 (12%)
Frame = +3
Query: 255 WLSPIFKSPM---VD----FGYDIANFYEIHHEYGTMEDFEALLKKANELDIKVVLDLVP 413
W+SPIF++ +D GY N YE++H +GT E+F L+++ ++ DI ++LD+
Sbjct: 87 WISPIFENVEGNDIDGSSYHGYWTTNLYELNHHFGTKEEFMELIQELHKRDIWILLDVAI 146
Query: 414 NHTS----NESVWFQEALNGNE-KYYNYFVWED 497
N + E + F++ + N+ +++ W D
Sbjct: 147 NSMAINGPLEQMSFEKVIPFNDASFFHPHCWVD 179
>SPCC11E10.09c ||SPCC188.01c|alpha-amylase homolog
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 478
Score = 37.1 bits (82), Expect = 0.003
Identities = 25/92 (27%), Positives = 46/92 (50%), Gaps = 12/92 (13%)
Frame = +3
Query: 243 CRSCWLSPIFK--SPMVD-----FGYDIANFYEIHHEYGTMEDFEALLKKANELDIKVVL 401
C + W+SPI K S D GY + +++ +GT ED + L+ +E ++ ++
Sbjct: 68 CTAIWISPIVKNISETTDCGQAYHGYWAQDMTQLNENFGTEEDLKELVNAIHEKNMLCMV 127
Query: 402 DLVPN---HTSNESVWF--QEALNGNEKYYNY 482
D+V N H ++ V F + N + Y+N+
Sbjct: 128 DIVVNHMGHAGSKPVNFLLYQPFNSGKYYHNW 159
Score = 28.3 bits (60), Expect = 1.2
Identities = 22/67 (32%), Positives = 29/67 (43%), Gaps = 10/67 (14%)
Frame = +1
Query: 85 CSGIIIKNGEVQDWWETSILYQIYPRSFA-DSDG---DGIGDL------NGITSKLEYIK 234
CS + K W ++YQ+ FA D D G+L GIT L+YIK
Sbjct: 5 CSILPKKEPSYLKLWRKQVIYQVLTDRFALDEDNFYAKASGNLYLGGTWKGITRNLDYIK 64
Query: 235 ELGVGAV 255
LG A+
Sbjct: 65 SLGCTAI 71
>SPAC27E2.01 |||alpha-amylase homolog |Schizosaccharomyces pombe|chr
1|||Manual
Length = 491
Score = 35.5 bits (78), Expect = 0.008
Identities = 19/78 (24%), Positives = 41/78 (52%), Gaps = 7/78 (8%)
Frame = +3
Query: 216 KIGIYQRIRCRSCWLSPIFKSPMVDFGYDIA-------NFYEIHHEYGTMEDFEALLKKA 374
K+ Q + C + W+SPI K+ GY A + +++ +GT +D L+ +
Sbjct: 64 KLDYIQSLGCTAVWISPIVKNIEGVTGYGEAYHGYWAEDLTQLNPHFGTKQDLTELVDQL 123
Query: 375 NELDIKVVLDLVPNHTSN 428
++ ++ ++D+V NH ++
Sbjct: 124 HKRNMLCMIDIVVNHMAH 141
>SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase
Mok11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2397
Score = 32.7 bits (71), Expect = 0.055
Identities = 20/57 (35%), Positives = 28/57 (49%), Gaps = 2/57 (3%)
Frame = +1
Query: 121 DWWETSILYQIYPRSFADSDGDGIGDLNGITSKLEYIKELGVGAVGF--RRFSNRPW 285
D+ T Y IY F + GD+ G+ L+Y++ +GV AV F F N PW
Sbjct: 83 DFSGTRFEYDIYETEFRNG-----GDIIGVRQSLDYLQGMGVKAVYFAGTPFVNMPW 134
>SPCC63.02c |aah3||alpha-amylase homolog Aah3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 564
Score = 32.7 bits (71), Expect = 0.055
Identities = 23/74 (31%), Positives = 34/74 (45%), Gaps = 12/74 (16%)
Frame = +1
Query: 70 LLFVACSGIIIKNGEVQDWWETSILYQIYPRSFADSDGDGI------------GDLNGIT 213
LLF++ + I + N W I+YQI FA DG G GI
Sbjct: 8 LLFLSSALIHVANAGSNAEWRKRIIYQILTDRFAVDDGSTDNPCDPDANQYCGGTWKGIE 67
Query: 214 SKLEYIKELGVGAV 255
+KL+YI+++G A+
Sbjct: 68 NKLDYIEDMGFNAI 81
Score = 29.9 bits (64), Expect = 0.38
Identities = 19/75 (25%), Positives = 37/75 (49%), Gaps = 8/75 (10%)
Frame = +3
Query: 216 KIGIYQRIRCRSCWLSPIFKSPMVDF--------GYDIANFYEIHHEYGTMEDFEALLKK 371
K+ + + + W+SPI K+ D GY ++ ++ +GT +D +L+
Sbjct: 69 KLDYIEDMGFNAIWISPIDKNIEGDIDGAGYAYHGYWNTDYESLNEHFGTEDDLVSLITA 128
Query: 372 ANELDIKVVLDLVPN 416
A++ I V+LD + N
Sbjct: 129 AHKAGIWVMLDSIVN 143
>SPAC458.06 |||phosphoinositide binding protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 364
Score = 29.5 bits (63), Expect = 0.51
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = +2
Query: 416 SYIKRKCLVPRSSQWQRKILQLFRLGRRNH 505
S K PR + W+RK+L+L G+R H
Sbjct: 269 SEFKSSTTEPRQTHWKRKLLKLIDSGKRAH 298
>SPBC16A3.13 |meu7|aah4|alpha-amylase homolog
Aah4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 774
Score = 28.7 bits (61), Expect = 0.89
Identities = 12/46 (26%), Positives = 25/46 (54%)
Frame = +3
Query: 294 GYDIANFYEIHHEYGTMEDFEALLKKANELDIKVVLDLVPNHTSNE 431
G+ N+Y ++ +G ED + L + + +++D+ NHT +E
Sbjct: 103 GHRPKNYYRLNPHFGNEEDLKELSDALHGKGMYLMVDVAINHTISE 148
>SPCC757.12 |||alpha-amylase homolog |Schizosaccharomyces pombe|chr
3|||Manual
Length = 625
Score = 28.3 bits (60), Expect = 1.2
Identities = 25/78 (32%), Positives = 36/78 (46%), Gaps = 11/78 (14%)
Frame = +1
Query: 55 VCLLSLLFVACSGIIIKNGEVQDWWETSILYQIYPRSFA-DSDG-------DGI---GDL 201
+ L SL A G+ + + W I+YQ+ FA DSD D G
Sbjct: 6 IALFSLF--ALFGLPTSLAKSSEEWRDRIIYQVITDRFAVDSDNTPDCSFDDSSYCGGTW 63
Query: 202 NGITSKLEYIKELGVGAV 255
+GI SKL+YI+ +G A+
Sbjct: 64 SGIRSKLDYIQGMGFNAI 81
>SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor,
zf-fungal binuclear cluster type |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 977
Score = 27.9 bits (59), Expect = 1.6
Identities = 15/41 (36%), Positives = 20/41 (48%), Gaps = 1/41 (2%)
Frame = -1
Query: 278 RFENRRKPTAPTPNSLIYSNFEVIPFKSPIPS-PSLSAKDL 159
+F++ R P + IYSNF P + PS P S K L
Sbjct: 302 KFDSNRISQVPNSDYSIYSNFNNFPIANHAPSFPQSSVKKL 342
>SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 425
Score = 27.5 bits (58), Expect = 2.1
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = +2
Query: 416 SYIKRKCLVPRSSQWQRKILQLFRLGRRNHRE 511
+++ K + +S +WQ+ +LFRL RN RE
Sbjct: 77 AHLSSKEIWEKSGRWQKTGSELFRLHDRNDRE 108
>SPCC16A11.17 |cdc21|mcm4, SPCC24B10.01|MCM complex subunit
Cdc21|Schizosaccharomyces pombe|chr 3|||Manual
Length = 911
Score = 27.5 bits (58), Expect = 2.1
Identities = 11/39 (28%), Positives = 21/39 (53%)
Frame = -1
Query: 263 RKPTAPTPNSLIYSNFEVIPFKSPIPSPSLSAKDLG*IW 147
++P TP+SL++S + + F PS ++ + IW
Sbjct: 122 QRPGVSTPSSLLFSGSDALTFSQAHPSSEVADDTVRVIW 160
>SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog
Mde5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 513
Score = 27.1 bits (57), Expect = 2.7
Identities = 18/75 (24%), Positives = 34/75 (45%), Gaps = 7/75 (9%)
Frame = +3
Query: 216 KIGIYQRIRCRSCWLSPIFKS-------PMVDFGYDIANFYEIHHEYGTMEDFEALLKKA 374
K+ Q + + W+SPI K+ GY + Y ++ +GT +D L
Sbjct: 70 KLDYIQGMGFTAIWISPIIKNIEGRTKYGEAYHGYWPQDLYTLNPHFGTEQDLIDLADAL 129
Query: 375 NELDIKVVLDLVPNH 419
++ + +++D V NH
Sbjct: 130 HDRGMYLMVDTVVNH 144
>SPBC16D10.05 |mok13||alpha-1,3-glucan synthase
Mok13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2358
Score = 26.6 bits (56), Expect = 3.6
Identities = 14/49 (28%), Positives = 24/49 (48%), Gaps = 2/49 (4%)
Frame = +1
Query: 145 YQIYPRSFADSDGDGIGDLNGITSKLEYIKELGVGA--VGFRRFSNRPW 285
Y IY F + GD+ G+ L+Y++ +G+ + F N+PW
Sbjct: 90 YDIYETGFRNG-----GDIIGLKDSLDYLEIMGIKVIYIAGTPFLNQPW 133
>SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-glucan
synthase Ags1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 2410
Score = 26.6 bits (56), Expect = 3.6
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = +2
Query: 533 NNWLSHFRGSAWEYKEEVGKYYLHQFAV 616
N WLS G +W Y ++G +HQ+ +
Sbjct: 2209 NFWLSSLNGRSWSYLWDIGN--IHQWQI 2234
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 26.2 bits (55), Expect = 4.7
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = +3
Query: 258 LSPIFKSPMVDFGYDIANFYEIHHEYGTMEDFE 356
+ P K ++D D+A + EIH E + E+FE
Sbjct: 591 VDPEDKQAVLDAVEDVAEWLEIHGEDASKEEFE 623
>SPCC1795.08c |||histone acetyltransferase complex subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 985
Score = 26.2 bits (55), Expect = 4.7
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = -1
Query: 236 SLIYSNFEVIPFKSPIPSPSLSAKDL 159
SL S E+I KSPIPSP K++
Sbjct: 129 SLELSEEEIITSKSPIPSPETIHKNI 154
>SPAC19A8.11c |||recombination protein Irc6 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 246
Score = 25.8 bits (54), Expect = 6.3
Identities = 15/46 (32%), Positives = 23/46 (50%)
Frame = +2
Query: 542 LSHFRGSAWEYKEEVGKYYLHQFAVGQPDLNYRNQDVVDEMKNIIR 679
LS S E K++ G +YL + G PD+ + D + + N IR
Sbjct: 168 LSQVDKSFSEEKDDAGDFYLPERFKGSPDMQSTDLDNLMKEMNQIR 213
>SPCC18B5.03 |wee1||dual specificity protein kinase
Wee1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 877
Score = 25.8 bits (54), Expect = 6.3
Identities = 23/76 (30%), Positives = 34/76 (44%), Gaps = 4/76 (5%)
Frame = -1
Query: 269 NRRKPTAPTPNSLIYSNFEVIPFKSPIPSPSLSAKDLG*IWYKIE----VSHQS*TSPFF 102
N+R P PT N + N I PSP + +++ + + +E VS S +S F
Sbjct: 486 NQRCPATPTRNPFAFENTVSIHMDGRQPSP-IKSRNNNQMSFAMEEEADVSQPS-SSSFT 543
Query: 101 IMIPLHATKSKDSKHT 54
+ P T SK S T
Sbjct: 544 LSFPSALTSSKVSSST 559
>SPCC1795.11 |sum3|ded1, slh3, moc2|ATP-dependent RNA helicase
Sum3|Schizosaccharomyces pombe|chr 3|||Manual
Length = 636
Score = 25.4 bits (53), Expect = 8.3
Identities = 11/47 (23%), Positives = 24/47 (51%)
Frame = -1
Query: 326 VDFIKISNVVAKINHGRFENRRKPTAPTPNSLIYSNFEVIPFKSPIP 186
++F K ++ +++ G E + T+P NS + N ++ + P P
Sbjct: 148 INFEKYDDIPVEVSGGDIEPVNEFTSPPLNSHLLQNIKLSGYTQPTP 194
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,280,950
Number of Sequences: 5004
Number of extensions: 75436
Number of successful extensions: 261
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 238
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 259
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 339215786
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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