SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0494
         (722 letters)

Database: bee 
           438 sequences; 146,343 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

D79208-1|BAA11466.1|  567|Apis mellifera alpha-glucosidase protein.   107   8e-26
AB253417-1|BAE86928.1|  567|Apis mellifera alpha-glucosidase pro...   107   8e-26
AB253416-1|BAE86927.1|  580|Apis mellifera alpha-glucosidase pro...   107   1e-25
AB253415-1|BAE86926.1|  588|Apis mellifera alpha-glucosidase pro...    98   8e-23
AY463910-1|AAR24352.1|  843|Apis mellifera metabotropic glutamat...    21   8.9  
AB161181-1|BAD08343.1|  933|Apis mellifera metabotropic glutamat...    21   8.9  

>D79208-1|BAA11466.1|  567|Apis mellifera alpha-glucosidase protein.
          Length = 567

 Score =  107 bits (258), Expect = 8e-26
 Identities = 43/84 (51%), Positives = 61/84 (72%)
 Frame = +3

Query: 255 WLSPIFKSPMVDFGYDIANFYEIHHEYGTMEDFEALLKKANELDIKVVLDLVPNHTSNES 434
           WLSPI+ SPMVDFGYDI+N+ ++H  +GT+ D + L+  A+E  +K++LD VPNHTS++ 
Sbjct: 70  WLSPIYPSPMVDFGYDISNYTDVHPIFGTISDLDNLVSAAHEKGLKIILDFVPNHTSDQH 129

Query: 435 VWFQEALNGNEKYYNYFVWEDGII 506
            WFQ +L   E Y NY++W  G I
Sbjct: 130 EWFQLSLKNIEPYNNYYIWHPGKI 153



 Score = 95.1 bits (226), Expect = 6e-22
 Identities = 39/67 (58%), Positives = 49/67 (73%)
 Frame = +2

Query: 512 NGNRQPPNNWLSHFRGSAWEYKEEVGKYYLHQFAVGQPDLNYRNQDVVDEMKNIIRFWLG 691
           NG R PP NW+  F GSAW ++EE   YYLHQFA  QPDLNY N  V+D+M+N++RFWL 
Sbjct: 155 NGKRVPPTNWVGVFGGSAWSWREERQAYYLHQFAPEQPDLNYYNPVVLDDMQNVLRFWLR 214

Query: 692 KGIAGFQ 712
           +G  GF+
Sbjct: 215 RGFDGFR 221



 Score = 58.0 bits (134), Expect = 8e-11
 Identities = 24/39 (61%), Positives = 31/39 (79%)
 Frame = +1

Query: 130 ETSILYQIYPRSFADSDGDGIGDLNGITSKLEYIKELGV 246
           E  I+YQ+YPRSF DS+GDGIGD+ GI  KL++  E+GV
Sbjct: 28  EDLIVYQVYPRSFKDSNGDGIGDIEGIKEKLDHFLEMGV 66


>AB253417-1|BAE86928.1|  567|Apis mellifera alpha-glucosidase
           protein.
          Length = 567

 Score =  107 bits (258), Expect = 8e-26
 Identities = 43/84 (51%), Positives = 61/84 (72%)
 Frame = +3

Query: 255 WLSPIFKSPMVDFGYDIANFYEIHHEYGTMEDFEALLKKANELDIKVVLDLVPNHTSNES 434
           WLSPI+ SPMVDFGYDI+N+ ++H  +GT+ D + L+  A+E  +K++LD VPNHTS++ 
Sbjct: 70  WLSPIYPSPMVDFGYDISNYTDVHPIFGTISDLDNLVSAAHEKGLKIILDFVPNHTSDQH 129

Query: 435 VWFQEALNGNEKYYNYFVWEDGII 506
            WFQ +L   E Y NY++W  G I
Sbjct: 130 EWFQLSLKNIEPYNNYYIWHPGKI 153



 Score = 95.1 bits (226), Expect = 6e-22
 Identities = 39/67 (58%), Positives = 49/67 (73%)
 Frame = +2

Query: 512 NGNRQPPNNWLSHFRGSAWEYKEEVGKYYLHQFAVGQPDLNYRNQDVVDEMKNIIRFWLG 691
           NG R PP NW+  F GSAW ++EE   YYLHQFA  QPDLNY N  V+D+M+N++RFWL 
Sbjct: 155 NGKRVPPTNWVGVFGGSAWSWREERQAYYLHQFAPEQPDLNYYNPVVLDDMQNVLRFWLR 214

Query: 692 KGIAGFQ 712
           +G  GF+
Sbjct: 215 RGFDGFR 221



 Score = 58.0 bits (134), Expect = 8e-11
 Identities = 24/39 (61%), Positives = 31/39 (79%)
 Frame = +1

Query: 130 ETSILYQIYPRSFADSDGDGIGDLNGITSKLEYIKELGV 246
           E  I+YQ+YPRSF DS+GDGIGD+ GI  KL++  E+GV
Sbjct: 28  EDLIVYQVYPRSFKDSNGDGIGDIEGIKEKLDHFLEMGV 66


>AB253416-1|BAE86927.1|  580|Apis mellifera alpha-glucosidase
           protein.
          Length = 580

 Score =  107 bits (256), Expect = 1e-25
 Identities = 43/84 (51%), Positives = 62/84 (73%)
 Frame = +3

Query: 255 WLSPIFKSPMVDFGYDIANFYEIHHEYGTMEDFEALLKKANELDIKVVLDLVPNHTSNES 434
           WLSPI+KSP VDFGYDI+NF ++   YGT+ DF+ L+++A  L +KV+LD VPNH+S+E 
Sbjct: 70  WLSPIYKSPQVDFGYDISNFTDVDPVYGTLADFDRLVRRAKSLGLKVILDFVPNHSSHEH 129

Query: 435 VWFQEALNGNEKYYNYFVWEDGII 506
            WF++++   + Y  Y+VW D  I
Sbjct: 130 PWFKKSVQRIKPYDEYYVWRDARI 153



 Score =  105 bits (251), Expect = 6e-25
 Identities = 42/67 (62%), Positives = 53/67 (79%)
 Frame = +2

Query: 512 NGNRQPPNNWLSHFRGSAWEYKEEVGKYYLHQFAVGQPDLNYRNQDVVDEMKNIIRFWLG 691
           NG RQPPNNWLS F GSAW++ EE  +YYLHQFA GQPDLNYR+  +  EMKN++ FW+ 
Sbjct: 155 NGTRQPPNNWLSVFWGSAWQWNEERKQYYLHQFATGQPDLNYRSAALDQEMKNVLTFWMN 214

Query: 692 KGIAGFQ 712
           +G+ GF+
Sbjct: 215 RGVDGFR 221



 Score = 70.9 bits (166), Expect = 1e-14
 Identities = 31/68 (45%), Positives = 50/68 (73%)
 Frame = +1

Query: 52  TVCLLSLLFVACSGIIIKNGEVQDWWETSILYQIYPRSFADSDGDGIGDLNGITSKLEYI 231
           T+  ++ L +A S I   +    +W++ +++YQIYPRSF DSDGDGIGDLNGIT+++++I
Sbjct: 5   TIVTVACLLLAASPIDCVDA---NWYKNALVYQIYPRSFQDSDGDGIGDLNGITARMDHI 61

Query: 232 KELGVGAV 255
            ++G  A+
Sbjct: 62  ADIGADAL 69


>AB253415-1|BAE86926.1|  588|Apis mellifera alpha-glucosidase
           protein.
          Length = 588

 Score = 97.9 bits (233), Expect = 8e-23
 Identities = 42/88 (47%), Positives = 63/88 (71%), Gaps = 5/88 (5%)
 Frame = +3

Query: 255 WLSPIFKSPMVDFGYDIANFYEIHHEYGTMEDFEALLKKANELDIKVVLDLVPNHTSNES 434
           WLSPI +SPMVDFGYDI++F ++   +GT++D E L  +A + ++KV+LDLVPNHTS++ 
Sbjct: 68  WLSPINRSPMVDFGYDISDFKDVDPIFGTIKDLEDLTAEAKKQNLKVILDLVPNHTSDQH 127

Query: 435 VWFQEAL-----NGNEKYYNYFVWEDGI 503
            WFQ ++     N   KY +Y++W D +
Sbjct: 128 KWFQMSINNTNNNNTNKYKDYYIWVDPV 155



 Score = 89.0 bits (211), Expect = 4e-20
 Identities = 37/61 (60%), Positives = 45/61 (73%)
 Frame = +2

Query: 530 PNNWLSHFRGSAWEYKEEVGKYYLHQFAVGQPDLNYRNQDVVDEMKNIIRFWLGKGIAGF 709
           PNNWLS F G+ W + E   ++Y HQF   QPDLNYRN DV +EMKNI++FWL KGI GF
Sbjct: 168 PNNWLSVFNGTGWTFHEGRKQFYFHQFYKQQPDLNYRNSDVREEMKNIMKFWLDKGIDGF 227

Query: 710 Q 712
           +
Sbjct: 228 R 228



 Score = 65.7 bits (153), Expect = 4e-13
 Identities = 32/79 (40%), Positives = 45/79 (56%)
 Frame = +1

Query: 46  MKTVCLLSLLFVACSGIIIKNGEVQDWWETSILYQIYPRSFADSDGDGIGDLNGITSKLE 225
           MK++ ++ LL     G    N   + WW+ +I YQ+YPRSF DS+ DGIGDL GI  KL 
Sbjct: 1   MKSLVVVVLLLAVGLGAGQNN---KGWWKNAIFYQVYPRSFMDSNSDGIGDLKGIKDKLS 57

Query: 226 YIKELGVGAVGFRRFSNRP 282
           +  E G+ A+     +  P
Sbjct: 58  HFIESGITAIWLSPINRSP 76


>AY463910-1|AAR24352.1|  843|Apis mellifera metabotropic glutamate
           receptor 1 protein.
          Length = 843

 Score = 21.4 bits (43), Expect = 8.9
 Identities = 10/32 (31%), Positives = 16/32 (50%)
 Frame = +3

Query: 297 YDIANFYEIHHEYGTMEDFEALLKKANELDIK 392
           Y+I NF +  H       ++ + K  N LDI+
Sbjct: 400 YEILNFRKSEHNGTNGYQYQVVGKWFNSLDIR 431


>AB161181-1|BAD08343.1|  933|Apis mellifera metabotropic glutamate
           receptor protein.
          Length = 933

 Score = 21.4 bits (43), Expect = 8.9
 Identities = 10/32 (31%), Positives = 16/32 (50%)
 Frame = +3

Query: 297 YDIANFYEIHHEYGTMEDFEALLKKANELDIK 392
           Y+I NF +  H       ++ + K  N LDI+
Sbjct: 490 YEILNFRKSEHNGTNGYQYQVVGKWFNSLDIR 521


  Database: bee
    Posted date:  Oct 23, 2007  1:17 PM
  Number of letters in database: 146,343
  Number of sequences in database:  438
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 219,130
Number of Sequences: 438
Number of extensions: 5058
Number of successful extensions: 20
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 19
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 22413960
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -