Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0494
         (722 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / sta...    49   3e-06
At5g03650.1 68418.m00324 1,4-alpha-glucan branching enzyme / sta...    46   2e-05
At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s...    44   1e-04
At1g69830.1 68414.m08034 alpha-amylase, putative / 1,4-alpha-D-g...    38   0.005
At4g25000.1 68417.m03587 alpha-amylase, putative / 1,4-alpha-D-g...    36   0.036
At1g76130.1 68414.m08841 alpha-amylase, putative / 1,4-alpha-D-g...    36   0.036
At1g07510.1 68414.m00804 FtsH protease, putative similar to AAA-...    32   0.33 
At2g39930.1 68415.m04907 isoamylase, putative / starch debranchi...    31   1.0  
At4g09020.1 68417.m01489 isoamylase, putative / starch debranchi...    29   2.4  
At5g61760.1 68418.m07749 inositol polyphosphate 6-/3-/5-kinase 2...    29   3.1  
At5g64640.1 68418.m08124 pectinesterase family protein contains ...    27   9.5  
At2g31580.1 68415.m03858 expressed protein   contains Pfam profi...    27   9.5  

>At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / starch
           branching enzyme class II (SBE2-1) nearly identical to
           starch branching enzyme class II [Arabidopsis thaliana]
           GI:619939
          Length = 858

 Score = 49.2 bits (112), Expect = 3e-06
 Identities = 20/62 (32%), Positives = 38/62 (61%)
 Frame = +3

Query: 291 FGYDIANFYEIHHEYGTMEDFEALLKKANELDIKVVLDLVPNHTSNESVWFQEALNGNEK 470
           FGY + NF+     +GT +D ++L+ KA+EL + V++D+V +H S  ++   +  +G + 
Sbjct: 378 FGYHVTNFFAPSSRFGTPDDLKSLIDKAHELGLVVLMDIVHSHASKNTLDGLDMFDGTDG 437

Query: 471 YY 476
            Y
Sbjct: 438 QY 439


>At5g03650.1 68418.m00324 1,4-alpha-glucan branching enzyme / starch
           branching enzyme class II (SBE2-2) identical to starch
           branching enzyme class II [Arabidopsis thaliana]
           GI:726490
          Length = 716

 Score = 46.4 bits (105), Expect = 2e-05
 Identities = 19/62 (30%), Positives = 37/62 (59%)
 Frame = +3

Query: 291 FGYDIANFYEIHHEYGTMEDFEALLKKANELDIKVVLDLVPNHTSNESVWFQEALNGNEK 470
           FGY + NF+      GT E+ ++L+ +A+EL + V++D+V +H S  ++      +G + 
Sbjct: 254 FGYHVTNFFAPSSRCGTPEELKSLIDRAHELGLVVLMDIVHSHASKNTLDGLNMFDGTDA 313

Query: 471 YY 476
           +Y
Sbjct: 314 HY 315


>At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein
           similar to 1,4-alpha-glucan branching enzyme [Solanum
           tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme
           (EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum
           tuberosum} SP|P30924; contains Pfam profiles: PF00128
           Alpha amylase catalytic domain, PF02922 Isoamylase
           N-terminal domain
          Length = 777

 Score = 43.6 bits (98), Expect = 1e-04
 Identities = 20/64 (31%), Positives = 38/64 (59%), Gaps = 1/64 (1%)
 Frame = +3

Query: 294 GYDIANFYEIHHEYGTMEDFEALLKKANELDIKVVLDLVPNHTSNESVWFQEALNG-NEK 470
           GY + NF+     YGT +DF+ L+ +A+ L + V LD+V ++ + + +      +G N+ 
Sbjct: 298 GYRVTNFFAASSRYGTPDDFKRLVDEAHGLGLLVFLDIVHSYAAADQMVGLSLFDGSNDC 357

Query: 471 YYNY 482
           Y++Y
Sbjct: 358 YFHY 361


>At1g69830.1 68414.m08034 alpha-amylase, putative /
           1,4-alpha-D-glucan glucanohydrolase, putative similar to
           SP|P17859 Alpha-amylase precursor (EC 3.2.1.1)
           (1,4-alpha-D-glucan glucanohydrolase) {Vigna mungo},
           alpha-amylase [Malus x domestica] GI:7532799; contains
           Pfam profile PF00128: Alpha amylase, catalytic domain
          Length = 887

 Score = 38.3 bits (85), Expect = 0.005
 Identities = 19/55 (34%), Positives = 33/55 (60%)
 Frame = +3

Query: 255 WLSPIFKSPMVDFGYDIANFYEIHHEYGTMEDFEALLKKANELDIKVVLDLVPNH 419
           WL P  +S   + GY   + Y ++  YGT+++ +  +KK +++ IKV+ D V NH
Sbjct: 532 WLPPPTESVSPE-GYMPKDLYNLNSRYGTIDELKDTVKKFHKVGIKVLGDAVLNH 585


>At4g25000.1 68417.m03587 alpha-amylase, putative /
           1,4-alpha-D-glucan glucanohydrolase, putative similar to
           alpha-amylase from Vigna mungo SP|P17859, Ipomoea nil
           GI:21670851; contains Pfam profile PF00128: Alpha
           amylase, catalytic domain
          Length = 423

 Score = 35.5 bits (78), Expect = 0.036
 Identities = 19/60 (31%), Positives = 34/60 (56%), Gaps = 1/60 (1%)
 Frame = +3

Query: 255 WLSPIFKSPMVDFGYDIANFYEIHH-EYGTMEDFEALLKKANELDIKVVLDLVPNHTSNE 431
           WL P  +S   + GY     Y+++  +YG+  + ++L+K  N+  IK + D+V NH + E
Sbjct: 63  WLPPPSQSVAPE-GYLPGKLYDLNSSKYGSEAELKSLIKALNQKGIKALADIVINHRTAE 121


>At1g76130.1 68414.m08841 alpha-amylase, putative /
           1,4-alpha-D-glucan glucanohydrolase, putative strong
           similarity to alpha-amylase GI:7532799 from [Malus x
           domestica];contains Pfam profile PF00128: Alpha amylase,
           catalytic domain
          Length = 413

 Score = 35.5 bits (78), Expect = 0.036
 Identities = 17/57 (29%), Positives = 31/57 (54%)
 Frame = +3

Query: 249 SCWLSPIFKSPMVDFGYDIANFYEIHHEYGTMEDFEALLKKANELDIKVVLDLVPNH 419
           S WL P  +S +   GY   + Y ++  YG+    ++LL+K  +  ++ + D+V NH
Sbjct: 59  SAWLPPPSQS-LAPEGYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVINH 114


>At1g07510.1 68414.m00804 FtsH protease, putative similar to
           AAA-metalloprotease FtsH [Pisum sativum] GI:15021761;
           contains Pfam profiles PF01434: Peptidase family M41,
           PF00004: ATPase AAA family
          Length = 813

 Score = 32.3 bits (70), Expect = 0.33
 Identities = 16/47 (34%), Positives = 26/47 (55%)
 Frame = +3

Query: 315 YEIHHEYGTMEDFEALLKKANELDIKVVLDLVPNHTSNESVWFQEAL 455
           Y+ +   G++E FE  L++A E       D VP    +E++W+QE L
Sbjct: 222 YKYYFNIGSVESFEEKLEEAQEAIGVNSHDFVPVTYVSETIWYQELL 268


>At2g39930.1 68415.m04907 isoamylase, putative / starch debranching
           enzyme, putative similar to isoamylase from [Solanum
           tuberosum] GI:27728145, [Triticum aestivum] GI:17932898,
           [Hordeum vulgare] GI:21314275, [Oryza sativa]
           GI:3252794; contains Pfam profiles PF00128: Alpha
           amylase catalytic domain, PF02922: Isoamylase N-terminal
           domain
          Length = 783

 Score = 30.7 bits (66), Expect = 1.0
 Identities = 12/42 (28%), Positives = 24/42 (57%), Gaps = 3/42 (7%)
 Frame = +1

Query: 139 ILYQIYPRSFADSDGDGI---GDLNGITSKLEYIKELGVGAV 255
           ++Y+++ R F   +   I   G   G+  KL+++KELG+  +
Sbjct: 219 VIYEMHVRGFTRHESSKIEFPGTYQGVAEKLDHLKELGINCI 260


>At4g09020.1 68417.m01489 isoamylase, putative / starch debranching
           enzyme, putative similar to isoamylase isoform 3
           [Solanum tuberosum] GI:27728149, isoamylase [Oryza
           sativa] GI:3252794; contains Pfam profiles PF00128:
           Alpha amylase catalytic domain, PF02922: Isoamylase
           N-terminal domain
          Length = 764

 Score = 29.5 bits (63), Expect = 2.4
 Identities = 16/53 (30%), Positives = 28/53 (52%), Gaps = 8/53 (15%)
 Frame = +3

Query: 291 FGYDIANFYEIHHEYGTME--------DFEALLKKANELDIKVVLDLVPNHTS 425
           +GY   NF+     Y + E        +F+ ++K  +   I+V+LD+V NHT+
Sbjct: 307 WGYSTVNFFAPMSRYASGEGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTN 359


>At5g61760.1 68418.m07749 inositol polyphosphate 6-/3-/5-kinase 2b
           (IPK2b) contains Pfam domain, PF03770: inositol
           polyphosphate kinase
          Length = 300

 Score = 29.1 bits (62), Expect = 3.1
 Identities = 11/27 (40%), Positives = 19/27 (70%)
 Frame = +1

Query: 67  SLLFVACSGIIIKNGEVQDWWETSILY 147
           S ++  C+GI+ +  E++DW+ET  LY
Sbjct: 191 SEVYGGCNGILAQLLELKDWFETQTLY 217


>At5g64640.1 68418.m08124 pectinesterase family protein contains
           Pfam profile: PF01095 pectinesterase
          Length = 602

 Score = 27.5 bits (58), Expect = 9.5
 Identities = 15/47 (31%), Positives = 20/47 (42%), Gaps = 1/47 (2%)
 Frame = +1

Query: 175 SDGDGIGDLN-GITSKLEYIKELGVGAVGFRRFSNRPWLILATTLLI 312
           S   G   LN  I    EY+KE      G + F  RPW   + T+ +
Sbjct: 483 SQSTGFVFLNCSINGTEEYMKEFQANPEGHKNFLGRPWKEFSRTVFV 529


>At2g31580.1 68415.m03858 expressed protein   contains Pfam profile:
           PF04446 family of unknown function (DUF549)
          Length = 567

 Score = 27.5 bits (58), Expect = 9.5
 Identities = 10/27 (37%), Positives = 17/27 (62%)
 Frame = +3

Query: 303 IANFYEIHHEYGTMEDFEALLKKANEL 383
           +  F +IH  YG  +++  +LKK +EL
Sbjct: 375 LEEFEDIHFAYGVSDEYSFVLKKESEL 401


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,746,056
Number of Sequences: 28952
Number of extensions: 380358
Number of successful extensions: 1060
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1026
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1060
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1575119672
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)