Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0489
(792 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g16150.1 68416.m02039 L-asparaginase, putative / L-asparagine... 71 1e-12
At5g08100.1 68418.m00945 L-asparaginase / L-asparagine amidohydr... 67 1e-11
At5g08100.2 68418.m00944 L-asparaginase / L-asparagine amidohydr... 62 3e-10
At5g61540.2 68418.m07723 L-asparaginase, putative / L-asparagine... 59 3e-09
At5g61540.1 68418.m07722 L-asparaginase, putative / L-asparagine... 59 3e-09
At4g00590.1 68417.m00083 asparaginase 2 family protein very low ... 37 0.018
At4g13530.2 68417.m02110 expressed protein predicted protein, Ar... 28 6.2
At4g13530.1 68417.m02109 expressed protein predicted protein, Ar... 28 6.2
At1g18450.1 68414.m02302 actin-related protein 4 (ARP4) neary id... 28 6.2
At5g02510.1 68418.m00185 hypothetical protein 28 8.2
At3g46620.1 68416.m05061 zinc finger (C3HC4-type RING finger) fa... 28 8.2
At2g01050.1 68415.m00010 hypothetical protein 28 8.2
>At3g16150.1 68416.m02039 L-asparaginase, putative / L-asparagine
amidohydrolase, putative similar to Swiss-Prot:P30364
L-asparaginase (EC 3.5.1.1) (L-asparagine
amidohydrolase) [Lupinus angustifolius]
Length = 325
Score = 70.5 bits (165), Expect = 1e-12
Identities = 39/83 (46%), Positives = 53/83 (63%)
Frame = +3
Query: 258 MELDENFNAGYGSVLNLNGEVEMEASIMWGQDLISGAVTLIKEFQHPISIARKVLTDTPH 437
+E D FN+G GS L G VEMEASIM G GAV+ I ++PIS+AR V+ +PH
Sbjct: 58 LETDPLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKSPH 117
Query: 438 SFLGGNGAKLFALEKGFQQVPPE 506
S+L +GA+ FA ++G + V E
Sbjct: 118 SYLAFSGAEDFARKQGVEIVDNE 140
Score = 61.7 bits (143), Expect = 5e-10
Identities = 26/44 (59%), Positives = 31/44 (70%)
Frame = +1
Query: 616 CGTIDADGHIAVATSTGGINGKMVGRIGDTPLIGGGTYADDNVG 747
C +D GH A TSTGG+ KM+GRIGD+PLIG GTYA + G
Sbjct: 198 CVVVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYASEFCG 241
>At5g08100.1 68418.m00945 L-asparaginase / L-asparagine
amidohydrolase identical to Swiss-Prot:P50287
L-asparaginase (EC 3.5.1.1) (L-asparagine
amidohydrolase) [Arabidopsis thaliana]
Length = 315
Score = 67.3 bits (157), Expect = 1e-11
Identities = 37/80 (46%), Positives = 49/80 (61%)
Frame = +3
Query: 258 MELDENFNAGYGSVLNLNGEVEMEASIMWGQDLISGAVTLIKEFQHPISIARKVLTDTPH 437
+E +FNAG GSVL G VEMEASIM G+ GAV+ + +PIS+AR V+ TPH
Sbjct: 59 LENHPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPH 118
Query: 438 SFLGGNGAKLFALEKGFQQV 497
+L + A+ FA G + V
Sbjct: 119 IYLAFDAAEAFARAHGVETV 138
Score = 62.5 bits (145), Expect = 3e-10
Identities = 33/59 (55%), Positives = 43/59 (72%), Gaps = 5/59 (8%)
Frame = +1
Query: 601 CGNSRCGTI-----DADGHIAVATSTGGINGKMVGRIGDTPLIGGGTYADDNVGGISTT 762
CG+S+ GT+ D+ G++A ATSTGG KMVGRIGDTP+IG GTYA +++ IS T
Sbjct: 176 CGDSQIGTVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYA-NHLCAISAT 233
>At5g08100.2 68418.m00944 L-asparaginase / L-asparagine
amidohydrolase identical to Swiss-Prot:P50287
L-asparaginase (EC 3.5.1.1) (L-asparagine
amidohydrolase) [Arabidopsis thaliana]
Length = 235
Score = 62.5 bits (145), Expect = 3e-10
Identities = 33/59 (55%), Positives = 43/59 (72%), Gaps = 5/59 (8%)
Frame = +1
Query: 601 CGNSRCGTI-----DADGHIAVATSTGGINGKMVGRIGDTPLIGGGTYADDNVGGISTT 762
CG+S+ GT+ D+ G++A ATSTGG KMVGRIGDTP+IG GTYA +++ IS T
Sbjct: 96 CGDSQIGTVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYA-NHLCAISAT 153
Score = 45.6 bits (103), Expect = 4e-05
Identities = 25/58 (43%), Positives = 35/58 (60%)
Frame = +3
Query: 324 MEASIMWGQDLISGAVTLIKEFQHPISIARKVLTDTPHSFLGGNGAKLFALEKGFQQV 497
MEASIM G+ GAV+ + +PIS+AR V+ TPH +L + A+ FA G + V
Sbjct: 1 MEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAFDAAEAFARAHGVETV 58
>At5g61540.2 68418.m07723 L-asparaginase, putative / L-asparagine
amidohydrolase, putative similar to Swiss-Prot:O02467
N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase
(Glycosylasparaginase)
(Aspartylglucosaminidase)(N4-(N-acetyl-beta-
glucosaminyl)-L-asparagine amidase) (AGA)[Spodoptera
frugiperda]
Length = 257
Score = 59.3 bits (137), Expect = 3e-09
Identities = 26/46 (56%), Positives = 30/46 (65%)
Frame = +1
Query: 625 IDADGHIAVATSTGGINGKMVGRIGDTPLIGGGTYADDNVGGISTT 762
ID GHIAV TST G K+ GR+GD P++G YADD VGG T
Sbjct: 128 IDRMGHIAVGTSTNGATYKIPGRVGDGPIVGSSAYADDEVGGCGAT 173
Score = 29.1 bits (62), Expect = 3.5
Identities = 14/41 (34%), Positives = 21/41 (51%)
Frame = +3
Query: 363 GAVTLIKEFQHPISIARKVLTDTPHSFLGGNGAKLFALEKG 485
GAV ++ + I A V+ + H+ L G GA FA+ G
Sbjct: 4 GAVAAMRYVKDGIRAAHLVMKYSQHTLLAGEGASAFAISMG 44
>At5g61540.1 68418.m07722 L-asparaginase, putative / L-asparagine
amidohydrolase, putative similar to Swiss-Prot:O02467
N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase
(Glycosylasparaginase)
(Aspartylglucosaminidase)(N4-(N-acetyl-beta-
glucosaminyl)-L-asparagine amidase) (AGA)[Spodoptera
frugiperda]
Length = 359
Score = 59.3 bits (137), Expect = 3e-09
Identities = 26/46 (56%), Positives = 30/46 (65%)
Frame = +1
Query: 625 IDADGHIAVATSTGGINGKMVGRIGDTPLIGGGTYADDNVGGISTT 762
ID GHIAV TST G K+ GR+GD P++G YADD VGG T
Sbjct: 230 IDRMGHIAVGTSTNGATYKIPGRVGDGPIVGSSAYADDEVGGCGAT 275
Score = 39.9 bits (89), Expect = 0.002
Identities = 24/75 (32%), Positives = 37/75 (49%)
Frame = +3
Query: 261 ELDENFNAGYGSVLNLNGEVEMEASIMWGQDLISGAVTLIKEFQHPISIARKVLTDTPHS 440
EL + G G + NGE ++A +M G + GAV ++ + I A V+ + H+
Sbjct: 72 ELRCDGTVGPGGSPDENGETMIDALVMDGVTMEVGAVAAMRYVKDGIRAAHLVMKYSQHT 131
Query: 441 FLGGNGAKLFALEKG 485
L G GA FA+ G
Sbjct: 132 LLAGEGASAFAISMG 146
>At4g00590.1 68417.m00083 asparaginase 2 family protein very low
similarity to glycosylasparaginase [SP|P20933] from Homo
sapiens; contains Pfam profile PF01112: Asparaginase 2
Length = 408
Score = 36.7 bits (81), Expect = 0.018
Identities = 16/36 (44%), Positives = 23/36 (63%)
Frame = +1
Query: 625 IDADGHIAVATSTGGINGKMVGRIGDTPLIGGGTYA 732
+D +GHIA +S+GGI K+ GR+G G G +A
Sbjct: 228 VDNEGHIACGSSSGGIAMKISGRVGLAATYGSGCWA 263
>At4g13530.2 68417.m02110 expressed protein predicted protein,
Arabidopsis thaliana
Length = 269
Score = 28.3 bits (60), Expect = 6.2
Identities = 23/75 (30%), Positives = 31/75 (41%)
Frame = +3
Query: 264 LDENFNAGYGSVLNLNGEVEMEASIMWGQDLISGAVTLIKEFQHPISIARKVLTDTPHSF 443
+D++ N G N G VE SI DLIS + HP+ ++ P
Sbjct: 101 VDDDVNNEMGIERNEVGIVEYSESIAQDMDLISSDERKEESLLHPVEGEGNSVSIDPGVK 160
Query: 444 LGGNGAKLFALEKGF 488
GG G + EKGF
Sbjct: 161 SGGGGGE----EKGF 171
>At4g13530.1 68417.m02109 expressed protein predicted protein,
Arabidopsis thaliana
Length = 270
Score = 28.3 bits (60), Expect = 6.2
Identities = 23/75 (30%), Positives = 31/75 (41%)
Frame = +3
Query: 264 LDENFNAGYGSVLNLNGEVEMEASIMWGQDLISGAVTLIKEFQHPISIARKVLTDTPHSF 443
+D++ N G N G VE SI DLIS + HP+ ++ P
Sbjct: 101 VDDDVNNEMGIERNEVGIVEYSESIAQDMDLISSDERKEESLLHPVEGEGNSVSIDPGVK 160
Query: 444 LGGNGAKLFALEKGF 488
GG G + EKGF
Sbjct: 161 SGGGGGE----EKGF 171
>At1g18450.1 68414.m02302 actin-related protein 4 (ARP4) neary
identical to actin-related protein 4 (ARP4) [Arabidopsis
thaliana] GI:21427463; contains Pfam profile PF00022:
Actin; supporting cDNA gi|21427462|gb|AF507912.1|
Length = 441
Score = 28.3 bits (60), Expect = 6.2
Identities = 12/46 (26%), Positives = 23/46 (50%)
Frame = -3
Query: 364 PEIRS*PHIILASISTSPFKFNTEPYPALKFSSNSIEQQQLQLNLK 227
P +R PH+++ SI+ E Y ++ + + QQL+ L+
Sbjct: 332 PSVRGLPHMVMESINKCDVDIRRELYSSILLAGGTSSMQQLKERLE 377
>At5g02510.1 68418.m00185 hypothetical protein
Length = 179
Score = 27.9 bits (59), Expect = 8.2
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = +2
Query: 512 ISESAKEALNDFLNKGDDQRTEIG 583
+ E AKE+L+ L GD RTE G
Sbjct: 7 VFEEAKESLSSILFNGDSDRTEAG 30
>At3g46620.1 68416.m05061 zinc finger (C3HC4-type RING finger)
family protein contains Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 395
Score = 27.9 bits (59), Expect = 8.2
Identities = 12/43 (27%), Positives = 21/43 (48%)
Frame = +1
Query: 97 AMKPIIIVHGGAGDISESRIQGKFDGVKVAVRAGYEKLMNGGS 225
+ P+I++HGG G + R++ + R YE + GS
Sbjct: 104 SFNPVIVLHGGGGGGAGERVENEEGDGATRERRAYEFYYDDGS 146
>At2g01050.1 68415.m00010 hypothetical protein
Length = 515
Score = 27.9 bits (59), Expect = 8.2
Identities = 15/48 (31%), Positives = 21/48 (43%)
Frame = -3
Query: 502 GGTCWKPFSSANSLAPFPPKKECGVSVKTFRAILIGCWNSLISVTAPE 359
GG WK +L P PK++ G + K R ++ G SV E
Sbjct: 393 GGFKWKRNGGMKALEPIGPKQKHGAANKPARGLIFGPTKDANSVPVGE 440
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,397,454
Number of Sequences: 28952
Number of extensions: 347681
Number of successful extensions: 953
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 898
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 947
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1785055200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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