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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0433
         (632 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_26408| Best HMM Match : Lipase (HMM E-Value=0)                      62   4e-10
SB_19823| Best HMM Match : No HMM Matches (HMM E-Value=.)              60   1e-09
SB_43441| Best HMM Match : Lipase (HMM E-Value=8.19998e-41)            50   2e-06
SB_7713| Best HMM Match : Lipase (HMM E-Value=0.0064)                  48   5e-06
SB_43904| Best HMM Match : Lipase (HMM E-Value=5e-07)                  36   0.027
SB_20514| Best HMM Match : No HMM Matches (HMM E-Value=.)              31   1.0  
SB_5209| Best HMM Match : No HMM Matches (HMM E-Value=.)               30   1.8  
SB_30050| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   1.8  
SB_58542| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   2.4  
SB_34654| Best HMM Match : zf-C2H2 (HMM E-Value=8e-31)                 29   4.1  
SB_22961| Best HMM Match : Abhydrolase_1 (HMM E-Value=0.043)           29   4.1  
SB_38976| Best HMM Match : BACK (HMM E-Value=3.09967e-42)              28   5.5  
SB_27171| Best HMM Match : No HMM Matches (HMM E-Value=.)              27   9.6  

>SB_26408| Best HMM Match : Lipase (HMM E-Value=0)
          Length = 714

 Score = 62.1 bits (144), Expect = 4e-10
 Identities = 44/119 (36%), Positives = 60/119 (50%), Gaps = 14/119 (11%)
 Frame = +2

Query: 308 VHIVGFNLGAHVAGVTGRNLEGK---VARITGLDPSARDW---ENNVLRLGTNDAQLR*S 469
           VH++GF+ GAHVAG  GR ++ +   + RIT LDP+A  W    +  +RL T+DA L   
Sbjct: 191 VHVIGFSFGAHVAGYVGRRMKKRGRMIDRITALDPAAM-WFHKHHEDVRLDTSDA-LFVD 248

Query: 470 YSHRRIWCQQKRLGIAIGHIDFFVNGRLVQPGCTN--------NLCSHNRAYEVFAATI 622
             H         +   IGH DF+ NG   QPGC N          C H RA  +F  ++
Sbjct: 249 VIHT---SADYGITSTIGHADFYPNGGKKQPGCDNFFRGFSSYLFCGHKRAPALFTTSL 304



 Score = 34.3 bits (75), Expect = 0.083
 Identities = 23/63 (36%), Positives = 34/63 (53%), Gaps = 8/63 (12%)
 Frame = +3

Query: 75  FNNNWDIVVIIHGHSG-TATTTINPIV-------KDAFLRSGNYNVIVVDWSSFSLSTYS 230
           FN +   V+IIHG +G T  T+I   V       K+  L  G++NVI+VDW   +   ++
Sbjct: 101 FNASRRTVIIIHGFAGFTTLTSIRHEVNWWGFPMKNELLWEGDFNVIIVDWMRGAWFPFT 160

Query: 231 TAV 239
            AV
Sbjct: 161 RAV 163


>SB_19823| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 940

 Score = 60.1 bits (139), Expect = 1e-09
 Identities = 44/118 (37%), Positives = 63/118 (53%), Gaps = 13/118 (11%)
 Frame = +2

Query: 311  HIVGFNLGAHVAGVTGRNLE---GKVARITGLDPSARDWEN-NV-LRLGTNDAQLR*SYS 475
            ++VGF+LGAH++G  GR +     K+ RITGLDP++  + N +V +RL  +DA       
Sbjct: 690  YLVGFSLGAHISGYVGRRIAKTGQKLNRITGLDPASIHFVNAHVDVRLDPSDADFV-DVM 748

Query: 476  HRRIWCQQKRLGIAIGHIDFFVNGRLVQPGCTNNL--------CSHNRAYEVFAATIT 625
            H  +           GHIDF+ NG   QPGC + L        C H RA E +A ++T
Sbjct: 749  HTDM--DLAGTPTVSGHIDFYPNGGKKQPGCRDLLDGPINYVICDHMRAPEYYAESVT 804


>SB_43441| Best HMM Match : Lipase (HMM E-Value=8.19998e-41)
          Length = 291

 Score = 49.6 bits (113), Expect = 2e-06
 Identities = 38/116 (32%), Positives = 62/116 (53%), Gaps = 10/116 (8%)
 Frame = +2

Query: 254 LGSSIATFLKN-LKLPLNK----VHIVGFNLGAHVAGVTGRNLE--GK-VARITGLDPSA 409
           +G+ +A F+K  L L  ++     H +GF+LGAH++G  G+ L+  G+ + RITGLDP+ 
Sbjct: 150 VGAQVAAFMKTILSLSGSREGGAFHSIGFSLGAHISGYVGQRLKRIGRHLDRITGLDPAT 209

Query: 410 RDWENNV--LRLGTNDAQLR*SYSHRRIWCQQKRLGIAIGHIDFFVNGRLVQPGCT 571
             ++     +RL   DAQ         ++     +    GH+DF+ NG   Q GC+
Sbjct: 210 LMFKGEAPDVRLDRLDAQFVDVIHTSYVF----GITAPHGHMDFYPNGGTSQRGCS 261



 Score = 27.5 bits (58), Expect = 9.6
 Identities = 13/30 (43%), Positives = 17/30 (56%)
 Frame = +3

Query: 150 VKDAFLRSGNYNVIVVDWSSFSLSTYSTAV 239
           +KD  LR  + NVI+VDW   +   Y  AV
Sbjct: 115 MKDELLRKSDDNVIIVDWIRGAKIPYVRAV 144


>SB_7713| Best HMM Match : Lipase (HMM E-Value=0.0064)
          Length = 131

 Score = 48.4 bits (110), Expect = 5e-06
 Identities = 22/47 (46%), Positives = 28/47 (59%)
 Frame = +2

Query: 251 RLGSSIATFLKNLKLPLNKVHIVGFNLGAHVAGVTGRNLEGKVARIT 391
           +L   I T  +     L +VH++G +LGAHVAG  G  L GKV RIT
Sbjct: 84  QLAELITTIQRVFDFDLRRVHLIGHSLGAHVAGYAGERLSGKVGRIT 130



 Score = 33.1 bits (72), Expect = 0.19
 Identities = 15/45 (33%), Positives = 24/45 (53%)
 Frame = +3

Query: 75  FNNNWDIVVIIHGHSGTATTTINPIVKDAFLRSGNYNVIVVDWSS 209
           F  +  +V+IIHG   +   +   +++D  L+    NVI VDW S
Sbjct: 19  FRGDRKLVLIIHGFMQSGNVSWIRVMRDELLKREPMNVITVDWQS 63


>SB_43904| Best HMM Match : Lipase (HMM E-Value=5e-07)
          Length = 408

 Score = 35.9 bits (79), Expect = 0.027
 Identities = 20/43 (46%), Positives = 25/43 (58%), Gaps = 8/43 (18%)
 Frame = +2

Query: 521 GHIDFFVNGRLVQPGC-------TNNL-CSHNRAYEVFAATIT 625
           GHIDF+ NG   QPGC       +N L C H RA++ F  +IT
Sbjct: 43  GHIDFYPNGGKNQPGCRDIADGPSNALKCDHVRAHDYFTESIT 85


>SB_20514| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 250

 Score = 30.7 bits (66), Expect = 1.0
 Identities = 18/60 (30%), Positives = 28/60 (46%), Gaps = 1/60 (1%)
 Frame = +2

Query: 188 YRCG-LVIIQSINIFNGSDGCDRLGSSIATFLKNLKLPLNKVHIVGFNLGAHVAGVTGRN 364
           Y+ G L+ IQ+I  F G D    L     +++ N+K+P  +   + F  G       GRN
Sbjct: 80  YKDGKLLAIQNIKKFLGEDSIQELIDFCLSYMSNIKIPKKRGTFIEFRHGLINVSPIGRN 139


>SB_5209| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 67

 Score = 29.9 bits (64), Expect = 1.8
 Identities = 15/31 (48%), Positives = 21/31 (67%), Gaps = 4/31 (12%)
 Frame = +2

Query: 314 IVGFNLGAHVAGVTG---RNLEG-KVARITG 394
           ++GF+LG HVAG  G   +N  G K+ RI+G
Sbjct: 21  VIGFSLGGHVAGYAGSRIKNTTGLKLGRISG 51


>SB_30050| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 161

 Score = 29.9 bits (64), Expect = 1.8
 Identities = 12/46 (26%), Positives = 26/46 (56%)
 Frame = +3

Query: 66  AAPFNNNWDIVVIIHGHSGTATTTINPIVKDAFLRSGNYNVIVVDW 203
           A+ ++      VI HG++ + +T+    ++ A L+  + NV++ DW
Sbjct: 95  ASTYDGKKRTFVIAHGYTESGSTSWVGHMRQALLQKDDVNVVITDW 140


>SB_58542| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 268

 Score = 29.5 bits (63), Expect = 2.4
 Identities = 12/47 (25%), Positives = 24/47 (51%)
 Frame = +2

Query: 266 IATFLKNLKLPLNKVHIVGFNLGAHVAGVTGRNLEGKVARITGLDPS 406
           + +FL  + +   K H+ G ++G H+ GV       +VA +  + P+
Sbjct: 120 LLSFLHAVGVNKRKFHLAGISMGGHIVGVYAAQHPARVASVIMMCPA 166


>SB_34654| Best HMM Match : zf-C2H2 (HMM E-Value=8e-31)
          Length = 624

 Score = 28.7 bits (61), Expect = 4.1
 Identities = 12/34 (35%), Positives = 19/34 (55%)
 Frame = +3

Query: 42  HFPTGNDPAAPFNNNWDIVVIIHGHSGTATTTIN 143
           HF  G++PA  ++NN D+   +  H  + T  IN
Sbjct: 569 HFKCGDNPAGIYDNNNDLETPVPPHRESKTARIN 602


>SB_22961| Best HMM Match : Abhydrolase_1 (HMM E-Value=0.043)
          Length = 183

 Score = 28.7 bits (61), Expect = 4.1
 Identities = 12/44 (27%), Positives = 22/44 (50%)
 Frame = +2

Query: 275 FLKNLKLPLNKVHIVGFNLGAHVAGVTGRNLEGKVARITGLDPS 406
           FL  + +   K H+ G ++G H+ GV       +VA +  + P+
Sbjct: 1   FLHAVGVNKRKFHLAGISMGGHIVGVYAAQHPARVASVIMMCPA 44


>SB_38976| Best HMM Match : BACK (HMM E-Value=3.09967e-42)
          Length = 603

 Score = 28.3 bits (60), Expect = 5.5
 Identities = 16/63 (25%), Positives = 29/63 (46%)
 Frame = +2

Query: 230 NGSDGCDRLGSSIATFLKNLKLPLNKVHIVGFNLGAHVAGVTGRNLEGKVARITGLDPSA 409
           +G   C   G+++ T + +   P  +V  V FN   ++ G T  +L   V  +   +P+A
Sbjct: 373 SGRVQCYNPGNNVWTTVASCLTPRCQVGAVAFNSHLYIIGGTTEHLSQPVNLVERYNPAA 432

Query: 410 RDW 418
             W
Sbjct: 433 DSW 435


>SB_27171| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 52

 Score = 27.5 bits (58), Expect = 9.6
 Identities = 11/25 (44%), Positives = 14/25 (56%)
 Frame = +2

Query: 524 HIDFFVNGRLVQPGCTNNLCSHNRA 598
           H+D  VNG  VQ       C+HN+A
Sbjct: 24  HLDKDVNGAYVQGSFIGGFCTHNKA 48


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,617,793
Number of Sequences: 59808
Number of extensions: 392602
Number of successful extensions: 1261
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 1139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1257
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1584657875
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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