BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0433 (632 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_26408| Best HMM Match : Lipase (HMM E-Value=0) 62 4e-10 SB_19823| Best HMM Match : No HMM Matches (HMM E-Value=.) 60 1e-09 SB_43441| Best HMM Match : Lipase (HMM E-Value=8.19998e-41) 50 2e-06 SB_7713| Best HMM Match : Lipase (HMM E-Value=0.0064) 48 5e-06 SB_43904| Best HMM Match : Lipase (HMM E-Value=5e-07) 36 0.027 SB_20514| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 1.0 SB_5209| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.8 SB_30050| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.8 SB_58542| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 2.4 SB_34654| Best HMM Match : zf-C2H2 (HMM E-Value=8e-31) 29 4.1 SB_22961| Best HMM Match : Abhydrolase_1 (HMM E-Value=0.043) 29 4.1 SB_38976| Best HMM Match : BACK (HMM E-Value=3.09967e-42) 28 5.5 SB_27171| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 9.6 >SB_26408| Best HMM Match : Lipase (HMM E-Value=0) Length = 714 Score = 62.1 bits (144), Expect = 4e-10 Identities = 44/119 (36%), Positives = 60/119 (50%), Gaps = 14/119 (11%) Frame = +2 Query: 308 VHIVGFNLGAHVAGVTGRNLEGK---VARITGLDPSARDW---ENNVLRLGTNDAQLR*S 469 VH++GF+ GAHVAG GR ++ + + RIT LDP+A W + +RL T+DA L Sbjct: 191 VHVIGFSFGAHVAGYVGRRMKKRGRMIDRITALDPAAM-WFHKHHEDVRLDTSDA-LFVD 248 Query: 470 YSHRRIWCQQKRLGIAIGHIDFFVNGRLVQPGCTN--------NLCSHNRAYEVFAATI 622 H + IGH DF+ NG QPGC N C H RA +F ++ Sbjct: 249 VIHT---SADYGITSTIGHADFYPNGGKKQPGCDNFFRGFSSYLFCGHKRAPALFTTSL 304 Score = 34.3 bits (75), Expect = 0.083 Identities = 23/63 (36%), Positives = 34/63 (53%), Gaps = 8/63 (12%) Frame = +3 Query: 75 FNNNWDIVVIIHGHSG-TATTTINPIV-------KDAFLRSGNYNVIVVDWSSFSLSTYS 230 FN + V+IIHG +G T T+I V K+ L G++NVI+VDW + ++ Sbjct: 101 FNASRRTVIIIHGFAGFTTLTSIRHEVNWWGFPMKNELLWEGDFNVIIVDWMRGAWFPFT 160 Query: 231 TAV 239 AV Sbjct: 161 RAV 163 >SB_19823| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 940 Score = 60.1 bits (139), Expect = 1e-09 Identities = 44/118 (37%), Positives = 63/118 (53%), Gaps = 13/118 (11%) Frame = +2 Query: 311 HIVGFNLGAHVAGVTGRNLE---GKVARITGLDPSARDWEN-NV-LRLGTNDAQLR*SYS 475 ++VGF+LGAH++G GR + K+ RITGLDP++ + N +V +RL +DA Sbjct: 690 YLVGFSLGAHISGYVGRRIAKTGQKLNRITGLDPASIHFVNAHVDVRLDPSDADFV-DVM 748 Query: 476 HRRIWCQQKRLGIAIGHIDFFVNGRLVQPGCTNNL--------CSHNRAYEVFAATIT 625 H + GHIDF+ NG QPGC + L C H RA E +A ++T Sbjct: 749 HTDM--DLAGTPTVSGHIDFYPNGGKKQPGCRDLLDGPINYVICDHMRAPEYYAESVT 804 >SB_43441| Best HMM Match : Lipase (HMM E-Value=8.19998e-41) Length = 291 Score = 49.6 bits (113), Expect = 2e-06 Identities = 38/116 (32%), Positives = 62/116 (53%), Gaps = 10/116 (8%) Frame = +2 Query: 254 LGSSIATFLKN-LKLPLNK----VHIVGFNLGAHVAGVTGRNLE--GK-VARITGLDPSA 409 +G+ +A F+K L L ++ H +GF+LGAH++G G+ L+ G+ + RITGLDP+ Sbjct: 150 VGAQVAAFMKTILSLSGSREGGAFHSIGFSLGAHISGYVGQRLKRIGRHLDRITGLDPAT 209 Query: 410 RDWENNV--LRLGTNDAQLR*SYSHRRIWCQQKRLGIAIGHIDFFVNGRLVQPGCT 571 ++ +RL DAQ ++ + GH+DF+ NG Q GC+ Sbjct: 210 LMFKGEAPDVRLDRLDAQFVDVIHTSYVF----GITAPHGHMDFYPNGGTSQRGCS 261 Score = 27.5 bits (58), Expect = 9.6 Identities = 13/30 (43%), Positives = 17/30 (56%) Frame = +3 Query: 150 VKDAFLRSGNYNVIVVDWSSFSLSTYSTAV 239 +KD LR + NVI+VDW + Y AV Sbjct: 115 MKDELLRKSDDNVIIVDWIRGAKIPYVRAV 144 >SB_7713| Best HMM Match : Lipase (HMM E-Value=0.0064) Length = 131 Score = 48.4 bits (110), Expect = 5e-06 Identities = 22/47 (46%), Positives = 28/47 (59%) Frame = +2 Query: 251 RLGSSIATFLKNLKLPLNKVHIVGFNLGAHVAGVTGRNLEGKVARIT 391 +L I T + L +VH++G +LGAHVAG G L GKV RIT Sbjct: 84 QLAELITTIQRVFDFDLRRVHLIGHSLGAHVAGYAGERLSGKVGRIT 130 Score = 33.1 bits (72), Expect = 0.19 Identities = 15/45 (33%), Positives = 24/45 (53%) Frame = +3 Query: 75 FNNNWDIVVIIHGHSGTATTTINPIVKDAFLRSGNYNVIVVDWSS 209 F + +V+IIHG + + +++D L+ NVI VDW S Sbjct: 19 FRGDRKLVLIIHGFMQSGNVSWIRVMRDELLKREPMNVITVDWQS 63 >SB_43904| Best HMM Match : Lipase (HMM E-Value=5e-07) Length = 408 Score = 35.9 bits (79), Expect = 0.027 Identities = 20/43 (46%), Positives = 25/43 (58%), Gaps = 8/43 (18%) Frame = +2 Query: 521 GHIDFFVNGRLVQPGC-------TNNL-CSHNRAYEVFAATIT 625 GHIDF+ NG QPGC +N L C H RA++ F +IT Sbjct: 43 GHIDFYPNGGKNQPGCRDIADGPSNALKCDHVRAHDYFTESIT 85 >SB_20514| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 250 Score = 30.7 bits (66), Expect = 1.0 Identities = 18/60 (30%), Positives = 28/60 (46%), Gaps = 1/60 (1%) Frame = +2 Query: 188 YRCG-LVIIQSINIFNGSDGCDRLGSSIATFLKNLKLPLNKVHIVGFNLGAHVAGVTGRN 364 Y+ G L+ IQ+I F G D L +++ N+K+P + + F G GRN Sbjct: 80 YKDGKLLAIQNIKKFLGEDSIQELIDFCLSYMSNIKIPKKRGTFIEFRHGLINVSPIGRN 139 >SB_5209| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 67 Score = 29.9 bits (64), Expect = 1.8 Identities = 15/31 (48%), Positives = 21/31 (67%), Gaps = 4/31 (12%) Frame = +2 Query: 314 IVGFNLGAHVAGVTG---RNLEG-KVARITG 394 ++GF+LG HVAG G +N G K+ RI+G Sbjct: 21 VIGFSLGGHVAGYAGSRIKNTTGLKLGRISG 51 >SB_30050| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 161 Score = 29.9 bits (64), Expect = 1.8 Identities = 12/46 (26%), Positives = 26/46 (56%) Frame = +3 Query: 66 AAPFNNNWDIVVIIHGHSGTATTTINPIVKDAFLRSGNYNVIVVDW 203 A+ ++ VI HG++ + +T+ ++ A L+ + NV++ DW Sbjct: 95 ASTYDGKKRTFVIAHGYTESGSTSWVGHMRQALLQKDDVNVVITDW 140 >SB_58542| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 268 Score = 29.5 bits (63), Expect = 2.4 Identities = 12/47 (25%), Positives = 24/47 (51%) Frame = +2 Query: 266 IATFLKNLKLPLNKVHIVGFNLGAHVAGVTGRNLEGKVARITGLDPS 406 + +FL + + K H+ G ++G H+ GV +VA + + P+ Sbjct: 120 LLSFLHAVGVNKRKFHLAGISMGGHIVGVYAAQHPARVASVIMMCPA 166 >SB_34654| Best HMM Match : zf-C2H2 (HMM E-Value=8e-31) Length = 624 Score = 28.7 bits (61), Expect = 4.1 Identities = 12/34 (35%), Positives = 19/34 (55%) Frame = +3 Query: 42 HFPTGNDPAAPFNNNWDIVVIIHGHSGTATTTIN 143 HF G++PA ++NN D+ + H + T IN Sbjct: 569 HFKCGDNPAGIYDNNNDLETPVPPHRESKTARIN 602 >SB_22961| Best HMM Match : Abhydrolase_1 (HMM E-Value=0.043) Length = 183 Score = 28.7 bits (61), Expect = 4.1 Identities = 12/44 (27%), Positives = 22/44 (50%) Frame = +2 Query: 275 FLKNLKLPLNKVHIVGFNLGAHVAGVTGRNLEGKVARITGLDPS 406 FL + + K H+ G ++G H+ GV +VA + + P+ Sbjct: 1 FLHAVGVNKRKFHLAGISMGGHIVGVYAAQHPARVASVIMMCPA 44 >SB_38976| Best HMM Match : BACK (HMM E-Value=3.09967e-42) Length = 603 Score = 28.3 bits (60), Expect = 5.5 Identities = 16/63 (25%), Positives = 29/63 (46%) Frame = +2 Query: 230 NGSDGCDRLGSSIATFLKNLKLPLNKVHIVGFNLGAHVAGVTGRNLEGKVARITGLDPSA 409 +G C G+++ T + + P +V V FN ++ G T +L V + +P+A Sbjct: 373 SGRVQCYNPGNNVWTTVASCLTPRCQVGAVAFNSHLYIIGGTTEHLSQPVNLVERYNPAA 432 Query: 410 RDW 418 W Sbjct: 433 DSW 435 >SB_27171| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 52 Score = 27.5 bits (58), Expect = 9.6 Identities = 11/25 (44%), Positives = 14/25 (56%) Frame = +2 Query: 524 HIDFFVNGRLVQPGCTNNLCSHNRA 598 H+D VNG VQ C+HN+A Sbjct: 24 HLDKDVNGAYVQGSFIGGFCTHNKA 48 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 19,617,793 Number of Sequences: 59808 Number of extensions: 392602 Number of successful extensions: 1261 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 1139 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1257 length of database: 16,821,457 effective HSP length: 79 effective length of database: 12,096,625 effective search space used: 1584657875 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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