Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0428
(748 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g18840.1 68416.m02392 pentatricopeptide (PPR) repeat-containi... 31 0.61
At3g59370.1 68416.m06621 expressed protein hypothetical protein ... 31 1.1
At1g25540.1 68414.m03171 phytochrome and flowering time regulato... 31 1.1
At4g21450.2 68417.m03102 vesicle-associated membrane family prot... 30 1.9
At4g21450.1 68417.m03103 vesicle-associated membrane family prot... 30 1.9
At5g31412.1 68418.m03722 hypothetical protein contains Pfam doma... 29 2.5
At5g14280.1 68418.m01670 DNA-binding storekeeper protein-related... 29 2.5
At2g45000.1 68415.m05603 expressed protein contains Pfam profile... 29 2.5
At5g62580.1 68418.m07855 expressed protein 29 3.3
At1g64790.1 68414.m07346 translational activator family protein ... 29 3.3
At4g25270.1 68417.m03635 pentatricopeptide (PPR) repeat-containi... 28 5.7
At3g25500.1 68416.m03171 formin homology 2 domain-containing pro... 28 5.7
At3g20540.1 68416.m02600 DNA-directed DNA polymerase family prot... 28 7.6
At1g56520.1 68414.m06499 disease resistance protein (TIR-NBS-LRR... 28 7.6
At5g63290.1 68418.m07943 coproporphyrinogen oxidase-related low ... 27 10.0
At5g48790.1 68418.m06037 expressed protein 27 10.0
At5g10560.1 68418.m01222 glycosyl hydrolase family 3 protein bet... 27 10.0
At5g09730.1 68418.m01127 glycosyl hydrolase family 3 protein bet... 27 10.0
At3g16800.2 68416.m02145 protein phosphatase 2C, putative / PP2C... 27 10.0
At3g16800.1 68416.m02146 protein phosphatase 2C, putative / PP2C... 27 10.0
>At3g18840.1 68416.m02392 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 698
Score = 31.5 bits (68), Expect = 0.61
Identities = 16/51 (31%), Positives = 29/51 (56%), Gaps = 1/51 (1%)
Frame = -2
Query: 354 EAYRVSREIGILNWSFDIFYEVYRINREIDVVNGI-SMSKIATLSTFSLNS 205
EAY +S E G D++ + YR+++ I+++ GI + K A + LN+
Sbjct: 522 EAYNISPETGHYTCMIDLYGKAYRLDKAIELMEGIDQVEKDAVILGAFLNA 572
>At3g59370.1 68416.m06621 expressed protein hypothetical protein
F12F1.4 - Arabidopsis thaliana, EMBL:AC002131
Length = 95
Score = 30.7 bits (66), Expect = 1.1
Identities = 21/69 (30%), Positives = 31/69 (44%)
Frame = +3
Query: 522 PVNIVDEPVEKTNEEIENDSEVGSISWVEYPAELDEDGNGLVIVDLPIEAQPEDLEKAQL 701
P + E EKTNE+ E + V E P ++E+ + + P E + E LE+ Q
Sbjct: 25 PEEVFVEDAEKTNEDEEEKAAV----ITETPTVVEEEKKAEEVTETPEEKKTEALEEKQT 80
Query: 702 VDLPVENVA 728
E VA
Sbjct: 81 EVAAAEEVA 89
>At1g25540.1 68414.m03171 phytochrome and flowering time regulatory
protein (PFT1) PMID: 12815435
Length = 836
Score = 30.7 bits (66), Expect = 1.1
Identities = 15/55 (27%), Positives = 26/55 (47%)
Frame = -1
Query: 466 QNQPGHRRPQLEHRRCLRSLQNQPGHRRPQLEHRGFLRSLQSQPGNRHSQLELRH 302
Q+ + PQL+ ++ Q Q H+ QL+H + Q Q +H +L+H
Sbjct: 707 QHLQQQQMPQLQQQQQQHQQQQQQQHQLSQLQHHQQQQQQQQQQQQQHQLTQLQH 761
Score = 29.5 bits (63), Expect = 2.5
Identities = 19/67 (28%), Positives = 34/67 (50%), Gaps = 2/67 (2%)
Frame = -1
Query: 511 KRRPQLEHRRCLRS*QNQPGHRRPQLEH--RRCLRSLQNQPGHRRPQLEHRGFLRSLQSQ 338
++ PQL+ ++ Q Q H+ QL+H ++ + Q Q H+ QL+H + Q+
Sbjct: 712 QQMPQLQQQQQQHQQQQQQQHQLSQLQHHQQQQQQQQQQQQQHQLTQLQHH-HQQQQQAS 770
Query: 337 PGNRHSQ 317
P N+ Q
Sbjct: 771 PLNQMQQ 777
>At4g21450.2 68417.m03102 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 212
Score = 29.9 bits (64), Expect = 1.9
Identities = 14/32 (43%), Positives = 19/32 (59%)
Frame = -1
Query: 691 FSKSSGCASIGRSTITSPFPSSSNSAGYSTQL 596
F SSG + S TSPFPS ++S+ S+ L
Sbjct: 23 FRNSSGHRNAASSAATSPFPSGASSSSTSSHL 54
>At4g21450.1 68417.m03103 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 295
Score = 29.9 bits (64), Expect = 1.9
Identities = 14/32 (43%), Positives = 19/32 (59%)
Frame = -1
Query: 691 FSKSSGCASIGRSTITSPFPSSSNSAGYSTQL 596
F SSG + S TSPFPS ++S+ S+ L
Sbjct: 23 FRNSSGHRNAASSAATSPFPSGASSSSTSSHL 54
>At5g31412.1 68418.m03722 hypothetical protein contains Pfam domain,
PF04937: Protein of unknown function (DUF 659)
Length = 433
Score = 29.5 bits (63), Expect = 2.5
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +3
Query: 606 EYPAELDEDGNGLVIVDLPIEAQPEDLEKAQLVDLPVE 719
E P+E DE+ NG + P E EDLE + D+ ++
Sbjct: 375 EGPSERDEEPNGEPVYQTPNEKPREDLESHEEHDITID 412
>At5g14280.1 68418.m01670 DNA-binding storekeeper protein-related
contains similarity to storekeeper protein [Solanum
tuberosum] gi|14268476|emb|CAC39398; contains PF04504:
Protein of unknown function, DUF573
Length = 572
Score = 29.5 bits (63), Expect = 2.5
Identities = 13/39 (33%), Positives = 20/39 (51%)
Frame = +3
Query: 549 EKTNEEIENDSEVGSISWVEYPAELDEDGNGLVIVDLPI 665
E N++ + S +SWVE P + + + I DLPI
Sbjct: 295 EAANDDYSSSSSSSIVSWVEIPISMSINIGEISIPDLPI 333
>At2g45000.1 68415.m05603 expressed protein contains Pfam profile:
PF05064 Nsp1-like C-terminal region
Length = 739
Score = 29.5 bits (63), Expect = 2.5
Identities = 22/50 (44%), Positives = 28/50 (56%), Gaps = 2/50 (4%)
Frame = -1
Query: 739 SSGSATFSTGRSTSW--AFSKSSGCASIGRSTITSPFPSSSNSAGYSTQL 596
SS S +TG S S+ A S S SI +T +SPF SS+SAG + L
Sbjct: 243 SSSSIFGATGSSPSFSVASSASGSSPSIFGATGSSPFFGSSSSAGSTPSL 292
>At5g62580.1 68418.m07855 expressed protein
Length = 615
Score = 29.1 bits (62), Expect = 3.3
Identities = 16/35 (45%), Positives = 19/35 (54%)
Frame = +3
Query: 90 HQSNPSKSRISQYRNTKASTLECTSSPTKSFKNSP 194
H S S SR+S YRN A T E +S + F SP
Sbjct: 541 HDSKYSASRMSTYRNRNAETTEIQNSRHR-FNGSP 574
>At1g64790.1 68414.m07346 translational activator family protein
similar to HsGCN1 [Homo sapiens] GI:2282576
Length = 2440
Score = 29.1 bits (62), Expect = 3.3
Identities = 16/60 (26%), Positives = 28/60 (46%)
Frame = +1
Query: 529 TSWMNQLKRRMRKSRMTVKLARSAGLNILLNWTKMETDLLLLIYLLRHNPKI*RKPSLLT 708
TS + L + + ++++V L L+ NW +L + LL+HNP SL +
Sbjct: 2224 TSMLGVLSQYLEAAQLSVLLQEVNDLSASQNWGARHGSVLCISSLLKHNPSTIMTSSLFS 2283
>At4g25270.1 68417.m03635 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 527
Score = 28.3 bits (60), Expect = 5.7
Identities = 14/40 (35%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = +3
Query: 57 LSSHSLERRPPHQSNPSKSRISQYRNTKASTLECTS-SPT 173
+SS S++++P H + R +QY N ++L T SPT
Sbjct: 16 VSSSSMKKKPRHHQQLKQHRQNQYNNNGFTSLSFTKPSPT 55
>At3g25500.1 68416.m03171 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 1051
Score = 28.3 bits (60), Expect = 5.7
Identities = 16/51 (31%), Positives = 26/51 (50%)
Frame = +3
Query: 57 LSSHSLERRPPHQSNPSKSRISQYRNTKASTLECTSSPTKSFKNSPWPGLN 209
+S E +PP S P + ++ YR ++ +L +S + KNS GLN
Sbjct: 306 MSPKRSEPKPPVISTPEPAELTDYRFVRSPSLS-LASLSSGLKNSDEVGLN 355
>At3g20540.1 68416.m02600 DNA-directed DNA polymerase family protein
similar to PolI-like DNA polymerase [Oryza sativa]
GI:19912795; contains Pfam profiles PF01612: 3'-5'
exonuclease, PF00476: DNA polymerase I family A
Length = 1034
Score = 27.9 bits (59), Expect = 7.6
Identities = 11/34 (32%), Positives = 18/34 (52%)
Frame = +3
Query: 108 KSRISQYRNTKASTLECTSSPTKSFKNSPWPGLN 209
K R +++RN K + PT+ F S WP ++
Sbjct: 590 KKRATKFRNIKLHRISDRPLPTEKFTASGWPSVS 623
>At1g56520.1 68414.m06499 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 897
Score = 27.9 bits (59), Expect = 7.6
Identities = 12/37 (32%), Positives = 23/37 (62%)
Frame = -2
Query: 462 ISRDIGVLNWSIDVVYEVYRISQDIGVLNWSIEVFYE 352
+S++ +WS+D + E+ + +DIG + + VFYE
Sbjct: 73 LSKNYASSSWSLDELLEILKCKEDIGQI--VMTVFYE 107
>At5g63290.1 68418.m07943 coproporphyrinogen oxidase-related low
similarity to coproporphyrinogen III oxidase from
Geobacillus stearothermophilus [GI:2104798]; contains
Pfam profile PF04055: radical SAM domain protein
Length = 484
Score = 27.5 bits (58), Expect = 10.0
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = -2
Query: 489 IDVVYEADRISRDIGVLNWSIDVV 418
+ VYEA ++ GV NWS+D++
Sbjct: 210 VSQVYEAIEFVKECGVENWSMDLI 233
>At5g48790.1 68418.m06037 expressed protein
Length = 316
Score = 27.5 bits (58), Expect = 10.0
Identities = 16/52 (30%), Positives = 25/52 (48%)
Frame = +1
Query: 172 RSHSRTRLGRD*IERKCRQRCDF*H*NSVDNVDFPVDSVNFVENVEAPVENA 327
+++ T+ R+ R C + + SVDNV FP D V + + VE A
Sbjct: 34 KNNDITKTNRNLKFRACSVSGGYNNNTSVDNVPFPRDYVELINQAKEAVEMA 85
>At5g10560.1 68418.m01222 glycosyl hydrolase family 3 protein
beta-xylosidase, Aspergllus nidulans, EMBL:ANXLND
Length = 792
Score = 27.5 bits (58), Expect = 10.0
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = +3
Query: 570 ENDSEVGSISWVEYPAE 620
+ND +GSI W+ YP E
Sbjct: 560 KNDPRIGSIIWIGYPGE 576
>At5g09730.1 68418.m01127 glycosyl hydrolase family 3 protein
beta-xylosidase, Hypocrea jecorina, EMBL:Z69257
Length = 773
Score = 27.5 bits (58), Expect = 10.0
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = +3
Query: 570 ENDSEVGSISWVEYPAELDEDGNGLVIVDL 659
+ND ++ SI WV YP E GL I D+
Sbjct: 546 KNDKKITSIMWVGYPGE----AGGLAIADV 571
>At3g16800.2 68416.m02145 protein phosphatase 2C, putative / PP2C,
putative similar to protein phosphatase-2C GB:AAC36699
from [Mesembryanthemum crystallinum]
Length = 351
Score = 27.5 bits (58), Expect = 10.0
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +3
Query: 615 AELDEDGNGLVIVDLPIEAQPEDLEKAQLV 704
A +DGNGLV V L ++ +P E+A+ +
Sbjct: 196 ATTSDDGNGLVPVQLSVDFKPNIPEEAERI 225
>At3g16800.1 68416.m02146 protein phosphatase 2C, putative / PP2C,
putative similar to protein phosphatase-2C GB:AAC36699
from [Mesembryanthemum crystallinum]
Length = 351
Score = 27.5 bits (58), Expect = 10.0
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +3
Query: 615 AELDEDGNGLVIVDLPIEAQPEDLEKAQLV 704
A +DGNGLV V L ++ +P E+A+ +
Sbjct: 196 ATTSDDGNGLVPVQLSVDFKPNIPEEAERI 225
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,658,271
Number of Sequences: 28952
Number of extensions: 297002
Number of successful extensions: 1152
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1078
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1150
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1653386488
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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