Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0425
(499 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_7713| Best HMM Match : Lipase (HMM E-Value=0.0064) 48 3e-06
SB_26408| Best HMM Match : Lipase (HMM E-Value=0) 48 6e-06
SB_43441| Best HMM Match : Lipase (HMM E-Value=8.19998e-41) 44 9e-05
SB_43904| Best HMM Match : Lipase (HMM E-Value=5e-07) 43 1e-04
SB_19823| Best HMM Match : No HMM Matches (HMM E-Value=.) 43 2e-04
SB_5209| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.2
SB_58542| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 1.6
SB_6080| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 1.6
SB_19258| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 2.1
SB_27171| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 2.8
SB_22961| Best HMM Match : Abhydrolase_1 (HMM E-Value=0.043) 29 2.8
SB_25694| Best HMM Match : RVT_1 (HMM E-Value=1.9e-22) 28 4.9
SB_42891| Best HMM Match : DUF537 (HMM E-Value=1.2e-23) 28 4.9
>SB_7713| Best HMM Match : Lipase (HMM E-Value=0.0064)
Length = 131
Score = 48.4 bits (110), Expect = 3e-06
Identities = 25/63 (39%), Positives = 35/63 (55%), Gaps = 4/63 (6%)
Frame = +2
Query: 17 LSTYSTAVMAVTGVGSSIATFLKNLK----LPLNKVHIVGFNLGAHVAGVTGRNLEGKVA 184
L+ Y A VG+ +A + ++ L +VH++G +LGAHVAG G L GKV
Sbjct: 68 LNLYHVAAGNTRVVGAQLAELITTIQRVFDFDLRRVHLIGHSLGAHVAGYAGERLSGKVG 127
Query: 185 RIT 193
RIT
Sbjct: 128 RIT 130
>SB_26408| Best HMM Match : Lipase (HMM E-Value=0)
Length = 714
Score = 47.6 bits (108), Expect = 6e-06
Identities = 29/85 (34%), Positives = 39/85 (45%), Gaps = 9/85 (10%)
Frame = +1
Query: 259 YVEVIHTDGSGVNKNGLGIAIGHIDFFVNGRLVQPGCTN--------NLCSHNRAYEVFA 414
+V+VIHT G+ IGH DF+ NG QPGC N C H RA +F
Sbjct: 246 FVDVIHTSAD----YGITSTIGHADFYPNGGKKQPGCDNFFRGFSSYLFCGHKRAPALFT 301
Query: 415 ATI-THGKHYGNQCSTEAEITANNC 486
++ T Y C +E + + NC
Sbjct: 302 TSLYTKTPLYSYPCRSEDDFNSGNC 326
Score = 46.0 bits (104), Expect = 2e-05
Identities = 32/86 (37%), Positives = 48/86 (55%), Gaps = 10/86 (11%)
Frame = +2
Query: 26 YSTAVMAVTGVGSSIATFLKNLK----LPLNKVHIVGFNLGAHVAGVTGRNLEGK---VA 184
++ AV VG+ A L+ L+ L VH++GF+ GAHVAG GR ++ + +
Sbjct: 159 FTRAVANTRLVGAQTARLLQILEERSGRKLAYVHVIGFSFGAHVAGYVGRRMKKRGRMID 218
Query: 185 RITGLDPSARDW---ENNVLRLGTND 253
RIT LDP+A W + +RL T+D
Sbjct: 219 RITALDPAAM-WFHKHHEDVRLDTSD 243
>SB_43441| Best HMM Match : Lipase (HMM E-Value=8.19998e-41)
Length = 291
Score = 43.6 bits (98), Expect = 9e-05
Identities = 28/69 (40%), Positives = 42/69 (60%), Gaps = 8/69 (11%)
Frame = +2
Query: 26 YSTAVMAVTGVGSSIATFLKN-LKLPLNK----VHIVGFNLGAHVAGVTGRNLE--GK-V 181
Y AV VG+ +A F+K L L ++ H +GF+LGAH++G G+ L+ G+ +
Sbjct: 140 YVRAVANTRLVGAQVAAFMKTILSLSGSREGGAFHSIGFSLGAHISGYVGQRLKRIGRHL 199
Query: 182 ARITGLDPS 208
RITGLDP+
Sbjct: 200 DRITGLDPA 208
Score = 30.7 bits (66), Expect = 0.70
Identities = 16/39 (41%), Positives = 22/39 (56%)
Frame = +1
Query: 256 QYVEVIHTDGSGVNKNGLGIAIGHIDFFVNGRLVQPGCT 372
Q+V+VIHT G+ GH+DF+ NG Q GC+
Sbjct: 227 QFVDVIHTS----YVFGITAPHGHMDFYPNGGTSQRGCS 261
>SB_43904| Best HMM Match : Lipase (HMM E-Value=5e-07)
Length = 408
Score = 43.2 bits (97), Expect = 1e-04
Identities = 26/65 (40%), Positives = 37/65 (56%), Gaps = 8/65 (12%)
Frame = +1
Query: 256 QYVEVIHTDGSGVNKNGLGIAIGHIDFFVNGRLVQPGC-------TNNL-CSHNRAYEVF 411
++V+V+HTD ++ G GHIDF+ NG QPGC +N L C H RA++ F
Sbjct: 24 EFVDVMHTD---MDFAGTSTQSGHIDFYPNGGKNQPGCRDIADGPSNALKCDHVRAHDYF 80
Query: 412 AATIT 426
+IT
Sbjct: 81 TESIT 85
>SB_19823| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 940
Score = 42.7 bits (96), Expect = 2e-04
Identities = 25/64 (39%), Positives = 35/64 (54%), Gaps = 8/64 (12%)
Frame = +1
Query: 259 YVEVIHTDGSGVNKNGLGIAIGHIDFFVNGRLVQPGCTNNL--------CSHNRAYEVFA 414
+V+V+HTD ++ G GHIDF+ NG QPGC + L C H RA E +A
Sbjct: 744 FVDVMHTD---MDLAGTPTVSGHIDFYPNGGKKQPGCRDLLDGPINYVICDHMRAPEYYA 800
Query: 415 ATIT 426
++T
Sbjct: 801 ESVT 804
Score = 41.9 bits (94), Expect = 3e-04
Identities = 26/74 (35%), Positives = 38/74 (51%), Gaps = 7/74 (9%)
Frame = +2
Query: 26 YSTAVMAVTGVGSSIATFLKNLKLPLNKV----HIVGFNLGAHVAGVTGRNLE---GKVA 184
Y A VG+ I +K L ++VGF+LGAH++G GR + K+
Sbjct: 657 YEQATANTRMVGAQITELIKFLNNQTGNTPASFYLVGFSLGAHISGYVGRRIAKTGQKLN 716
Query: 185 RITGLDPSARDWEN 226
RITGLDP++ + N
Sbjct: 717 RITGLDPASIHFVN 730
>SB_5209| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 67
Score = 29.9 bits (64), Expect = 1.2
Identities = 15/31 (48%), Positives = 21/31 (67%), Gaps = 4/31 (12%)
Frame = +2
Query: 116 IVGFNLGAHVAGVTG---RNLEG-KVARITG 196
++GF+LG HVAG G +N G K+ RI+G
Sbjct: 21 VIGFSLGGHVAGYAGSRIKNTTGLKLGRISG 51
>SB_58542| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 268
Score = 29.5 bits (63), Expect = 1.6
Identities = 12/47 (25%), Positives = 24/47 (51%)
Frame = +2
Query: 68 IATFLKNLKLPLNKVHIVGFNLGAHVAGVTGRNLEGKVARITGLDPS 208
+ +FL + + K H+ G ++G H+ GV +VA + + P+
Sbjct: 120 LLSFLHAVGVNKRKFHLAGISMGGHIVGVYAAQHPARVASVIMMCPA 166
>SB_6080| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 2101
Score = 29.5 bits (63), Expect = 1.6
Identities = 14/45 (31%), Positives = 20/45 (44%)
Frame = +1
Query: 331 DFFVNGRLVQPGCTNNLCSHNRAYEVFAATITHGKHYGNQCSTEA 465
D++ ++ G T+ C YE T GK YGN+C A
Sbjct: 1278 DYYAQCQVEDDGSTSCKCPIFCTYEYMPVCGTDGKTYGNKCEMRA 1322
>SB_19258| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 459
Score = 29.1 bits (62), Expect = 2.1
Identities = 21/58 (36%), Positives = 25/58 (43%), Gaps = 2/58 (3%)
Frame = +1
Query: 325 HID-FFVNGRLVQPGCTNNLCSHNRAYEVFAATITHGKHYGNQCSTEAEITANN-CRG 492
HID +NG +V PG + Y AT+T G Y EAE T CRG
Sbjct: 33 HIDQSSINGSVVPPGALVTILQKGVQYVEAEATVTEGVQY-----VEAEATVTEVCRG 85
>SB_27171| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 52
Score = 28.7 bits (61), Expect = 2.8
Identities = 15/37 (40%), Positives = 20/37 (54%), Gaps = 3/37 (8%)
Frame = +1
Query: 298 KNGLGIAIG---HIDFFVNGRLVQPGCTNNLCSHNRA 399
K+ G+ IG H+D VNG VQ C+HN+A
Sbjct: 12 KSSEGMYIGADLHLDKDVNGAYVQGSFIGGFCTHNKA 48
>SB_22961| Best HMM Match : Abhydrolase_1 (HMM E-Value=0.043)
Length = 183
Score = 28.7 bits (61), Expect = 2.8
Identities = 12/44 (27%), Positives = 22/44 (50%)
Frame = +2
Query: 77 FLKNLKLPLNKVHIVGFNLGAHVAGVTGRNLEGKVARITGLDPS 208
FL + + K H+ G ++G H+ GV +VA + + P+
Sbjct: 1 FLHAVGVNKRKFHLAGISMGGHIVGVYAAQHPARVASVIMMCPA 44
>SB_25694| Best HMM Match : RVT_1 (HMM E-Value=1.9e-22)
Length = 1797
Score = 27.9 bits (59), Expect = 4.9
Identities = 21/64 (32%), Positives = 34/64 (53%), Gaps = 2/64 (3%)
Frame = -2
Query: 258 LTSFVPSRSTLFSQSRAEGSRPVILATLPSKFLP--VTPAT*APRLKPTMCTLLSGNLRF 85
L +F +R++L S EG++PV L + + FLP P T + +P T+ S +R
Sbjct: 1176 LVTFCTARTSLISGLTIEGTQPVQLCSGRTIFLPKETVPET-EQQHRP--LTIASAVIRL 1232
Query: 84 FKKV 73
F K+
Sbjct: 1233 FHKI 1236
>SB_42891| Best HMM Match : DUF537 (HMM E-Value=1.2e-23)
Length = 2386
Score = 27.9 bits (59), Expect = 4.9
Identities = 24/64 (37%), Positives = 30/64 (46%), Gaps = 12/64 (18%)
Frame = +2
Query: 8 RFSLSTYSTAVMAVT----GVGSSIATFLKNLKLPLNK--VHI------VGFNLGAHVAG 151
RF L T ++A T G S ++T L L LP+ K HI + N G HV
Sbjct: 946 RFDLFTADLPIIATTPNRTGDESGLSTELTILNLPVGKDVAHIKSRLRQLSDNCGGHVIS 1005
Query: 152 VTGR 163
VTGR
Sbjct: 1006 VTGR 1009
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,473,461
Number of Sequences: 59808
Number of extensions: 291680
Number of successful extensions: 848
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 812
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 847
length of database: 16,821,457
effective HSP length: 77
effective length of database: 12,216,241
effective search space used: 1075029208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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