Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0416
         (737 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha...    46   4e-06
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch...    43   4e-05
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz...    39   6e-04
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch...    38   0.001
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac...    38   0.001
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc...    36   0.006
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch...    36   0.008
SPAC23A1.04c |mnl1||alpha mannosidase-like protein|Schizosacchar...    28   1.6  
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos...    27   2.8  
SPCC1259.13 |chk1|rad27|Chk1 protein kinase|Schizosaccharomyces ...    26   4.9  
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch...    26   6.4  
SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2 |Schizosacc...    25   8.5  
SPAC1D4.10 |||tRNA endonuclease|Schizosaccharomyces pombe|chr 1|...    25   8.5  

>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
           Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 147

 Score = 46.4 bits (105), Expect = 4e-06
 Identities = 28/81 (34%), Positives = 42/81 (51%)
 Frame = +2

Query: 197 QYTTQRPPPAERKGGKRLYFLNIIFKETYPFEPPFVRVVYPIISGGYVLVGGAICMELLT 376
           Q T   P  +   GG  ++FL+I F   YPF+PP V     I     +   G+IC+++L 
Sbjct: 34  QATIMGPADSPYAGG--VFFLSIHFPTDYPFKPPKVNFTTRIYHPN-INSNGSICLDILR 90

Query: 377 KQGWSSAYTVEAVIMQIAATL 439
            Q WS A T+  V++ I + L
Sbjct: 91  DQ-WSPALTISKVLLSICSLL 110


>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
           Hus5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 157

 Score = 43.2 bits (97), Expect = 4e-05
 Identities = 23/71 (32%), Positives = 36/71 (50%), Gaps = 1/71 (1%)
 Frame = +2

Query: 218 PPAERKGGKRLYFLNIIFKETYPFEPPFVRVVYPIISGGYVLVGGAICMELLT-KQGWSS 394
           P    +GG  LY L + F E YP  PP  R   P+     V   G +C+ +L  ++GW  
Sbjct: 49  PKTSWEGG--LYKLTMAFPEEYPTRPPKCRFTPPLFHPN-VYPSGTVCLSILNEEEGWKP 105

Query: 395 AYTVEAVIMQI 427
           A T++ +++ I
Sbjct: 106 AITIKQILLGI 116


>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
           E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 151

 Score = 39.1 bits (87), Expect = 6e-04
 Identities = 22/73 (30%), Positives = 36/73 (49%)
 Frame = +2

Query: 221 PAERKGGKRLYFLNIIFKETYPFEPPFVRVVYPIISGGYVLVGGAICMELLTKQGWSSAY 400
           PA+       + L + F E YP +PP V+ V  +     V   G +C+++L  + WS  Y
Sbjct: 43  PADTPFEDGTFKLVLSFDEQYPNKPPLVKFVSTMFHPN-VYANGELCLDILQNR-WSPTY 100

Query: 401 TVEAVIMQIAATL 439
            V A++  I + L
Sbjct: 101 DVAAILTSIQSLL 113


>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
           Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 148

 Score = 38.3 bits (85), Expect = 0.001
 Identities = 24/79 (30%), Positives = 38/79 (48%)
 Frame = +2

Query: 203 TTQRPPPAERKGGKRLYFLNIIFKETYPFEPPFVRVVYPIISGGYVLVGGAICMELLTKQ 382
           T + P  +  +GGK  + L +   + YP  PP VR +  I       + G IC+  L K+
Sbjct: 37  TMEGPQQSAYEGGK--FHLELFLPDEYPMMPPNVRFLTKIYHPNVDKL-GRICLSTL-KK 92

Query: 383 GWSSAYTVEAVIMQIAATL 439
            WS A  +  V++ I A +
Sbjct: 93  DWSPALQIRTVLLSIQALM 111


>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
           Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 160

 Score = 38.3 bits (85), Expect = 0.001
 Identities = 22/75 (29%), Positives = 37/75 (49%)
 Frame = +2

Query: 215 PPPAERKGGKRLYFLNIIFKETYPFEPPFVRVVYPIISGGYVLVGGAICMELLTKQGWSS 394
           P     +GG+  + L+I   E YP  PP V     I+        G +CM++L K  WS 
Sbjct: 44  PTETPYEGGQ--WVLDIHVHEGYPISPPSVYFQTKIVHPNISWTNGEVCMDIL-KTHWSP 100

Query: 395 AYTVEAVIMQIAATL 439
           A+++++  + I + L
Sbjct: 101 AWSLQSACLAIISLL 115


>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
           Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 227

 Score = 35.9 bits (79), Expect = 0.006
 Identities = 19/80 (23%), Positives = 37/80 (46%), Gaps = 2/80 (2%)
 Frame = +2

Query: 200 YTTQRPPPAERKGGKRLYFLNIIFKETYPFEPPFVRVVYPIISGGYVLVGGAICMEL--L 373
           Y    PP    +GG+  Y   +IF   YPF+PP +R++ P    G       +C+     
Sbjct: 39  YIITGPPDTPYEGGQ--YHGTLIFPPDYPFKPPAIRMITP---SGRFQTNTRLCLSFSDF 93

Query: 374 TKQGWSSAYTVEAVIMQIAA 433
             + W+ ++ V  +++ + +
Sbjct: 94  HPKSWNPSWMVSTILVGLVS 113


>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
           Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 217

 Score = 35.5 bits (78), Expect = 0.008
 Identities = 17/53 (32%), Positives = 28/53 (52%)
 Frame = +2

Query: 281 YPFEPPFVRVVYPIISGGYVLVGGAICMELLTKQGWSSAYTVEAVIMQIAATL 439
           YPF PP +     I         GAIC+++L  Q WS  YT+++ ++ + + L
Sbjct: 63  YPFRPPKMNFDTKIYHPNVSSQTGAICLDILKDQ-WSPVYTMKSALISLQSLL 114


>SPAC23A1.04c |mnl1||alpha mannosidase-like
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 787

 Score = 27.9 bits (59), Expect = 1.6
 Identities = 14/36 (38%), Positives = 20/36 (55%)
 Frame = +1

Query: 283 SIRAAFCACCLSYNLRRLCISRRCDMYGAINEARLV 390
           S+ + FC C +      LCI +   + GAIN AR+V
Sbjct: 3   SLHSIFCVCLIL-----LCIFKENSIVGAINSARMV 33


>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
           Mms2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 139

 Score = 27.1 bits (57), Expect = 2.8
 Identities = 20/80 (25%), Positives = 31/80 (38%), Gaps = 1/80 (1%)
 Frame = +2

Query: 221 PAERKGGKRLYFLNIIFKETYPFEPPFVRVVYPI-ISGGYVLVGGAICMELLTKQGWSSA 397
           PA      R+Y L I     YP  PP V  V  I + G     G     ++   + W   
Sbjct: 48  PAHSVHENRIYSLKIHCDANYPDAPPIVTFVSRINLPGVDGETGKVNPHKIDCLRHWKRE 107

Query: 398 YTVEAVIMQIAATLVKGKAR 457
           Y++E V++ +   +     R
Sbjct: 108 YSMETVLLDLKKEMASSSNR 127


>SPCC1259.13 |chk1|rad27|Chk1 protein kinase|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 496

 Score = 26.2 bits (55), Expect = 4.9
 Identities = 8/20 (40%), Positives = 16/20 (80%)
 Frame = -2

Query: 160 YSESFTNSMEYILFLKECER 101
           + E+ +N+ +Y+L+ K+CER
Sbjct: 213 WDEAISNTGDYLLYKKQCER 232


>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
           Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 184

 Score = 25.8 bits (54), Expect = 6.4
 Identities = 12/60 (20%), Positives = 29/60 (48%)
 Frame = +2

Query: 221 PAERKGGKRLYFLNIIFKETYPFEPPFVRVVYPIISGGYVLVGGAICMELLTKQGWSSAY 400
           P+E      ++ +++     YP++ P +  V  I       + G++C++++  Q WS  +
Sbjct: 39  PSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSVCLDVI-NQTWSPMF 97


>SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 758

 Score = 25.4 bits (53), Expect = 8.5
 Identities = 14/42 (33%), Positives = 18/42 (42%)
 Frame = +1

Query: 229 KKRREKTLFSQHYIQGNLSIRAAFCACCLSYNLRRLCISRRC 354
           KK  E     Q +I+     R A C+ CL  N  +L    RC
Sbjct: 396 KKMAEAEGLDQIFIEAGFDWREAGCSMCLGMNPDQLKPYERC 437


>SPAC1D4.10 |||tRNA endonuclease|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 809

 Score = 25.4 bits (53), Expect = 8.5
 Identities = 13/56 (23%), Positives = 25/56 (44%)
 Frame = +2

Query: 359 CMELLTKQGWSSAYTVEAVIMQIAATLVKGKARIQFSATKVVSQTQYSLAVHSRAS 526
           CME+    GW  AY+ +    +  A + K    +   AT  +  + + +A+  + S
Sbjct: 673 CMEITNSSGWKIAYSGDTRPSEDFANIAKDSTLLIHEAT--LEDSMHEIAIKKQHS 726


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,297,738
Number of Sequences: 5004
Number of extensions: 70620
Number of successful extensions: 188
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 182
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 187
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)