Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0398
(633 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g12030.1 68418.m01406 17.7 kDa class II heat shock protein 17... 43 1e-04
At5g12020.1 68418.m01405 17.6 kDa class II heat shock protein (... 42 3e-04
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 38 0.004
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 36 0.017
At5g51440.1 68418.m06377 23.5 kDa mitochondrial small heat shock... 36 0.022
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 36 0.030
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 35 0.039
At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein (HS... 34 0.068
At4g25200.1 68417.m03627 23.6 kDa mitochondrial small heat shock... 34 0.090
At1g63230.1 68414.m07147 pentatricopeptide (PPR) repeat-containi... 33 0.21
At3g05680.1 68416.m00634 expressed protein 31 0.84
At2g18220.1 68415.m02123 expressed protein contains Pfam domain ... 30 1.1
At3g18210.1 68416.m02316 oxidoreductase, 2OG-Fe(II) oxygenase fa... 30 1.5
At2g15630.1 68415.m01790 pentatricopeptide (PPR) repeat-containi... 29 1.9
At2g02160.1 68415.m00152 zinc finger (CCCH-type) family protein ... 29 2.6
At1g73770.1 68414.m08541 hypothetical protein 29 2.6
At3g26125.1 68416.m03258 cytochrome P450, putative 29 3.4
At2g41450.1 68415.m05121 GCN5-related N-acetyltransferase (GNAT)... 28 4.5
At1g24540.1 68414.m03089 cytochrome P450, putative similar to GB... 28 4.5
At5g37670.1 68418.m04537 15.7 kDa class I-related small heat sho... 28 5.9
At3g44610.1 68416.m04796 protein kinase family protein similar t... 28 5.9
At1g13140.1 68414.m01523 cytochrome P450 family protein similar ... 28 5.9
>At5g12030.1 68418.m01406 17.7 kDa class II heat shock protein 17.6A
(HSP17.7-CII) identical to heat shock protein 17.6A
GI:3256075 from [Arabidopsis thaliana]
Length = 156
Score = 43.2 bits (97), Expect = 1e-04
Identities = 29/80 (36%), Positives = 42/80 (52%), Gaps = 9/80 (11%)
Frame = +3
Query: 303 DEITVKASN-NTVVVEGKHEEKQDEHG---FISRQ-----FTRRYILPTGYEVNDLVSTL 455
DEI V+ N N +VV GK + E+ F+ + F R++ LP ++ + +S
Sbjct: 69 DEIQVQIENENVLVVSGKRQRDNKENEGVKFVRMERRMGKFMRKFQLPDNADL-EKISAA 127
Query: 456 SSDGVLTVTAPKRPPPNAAK 515
+DGVL VT PK PPP K
Sbjct: 128 CNDGVLKVTIPKLPPPEPKK 147
>At5g12020.1 68418.m01405 17.6 kDa class II heat shock protein
(HSP17.6-CII) identical to 17.6 kDa class II heat shock
protein SP:P29830 from [Arabidopsis thaliana]
Length = 155
Score = 41.9 bits (94), Expect = 3e-04
Identities = 30/92 (32%), Positives = 46/92 (50%), Gaps = 9/92 (9%)
Frame = +3
Query: 267 FEVILDDQQFTPDEITVKASNNTVVV---EGKHEEKQDEHGFISR------QFTRRYILP 419
+ ++D DEI V+ N+ V+V E + E K++E R +F R++ LP
Sbjct: 56 YAFVVDMPGIKGDEIKVQVENDNVLVVSGERQRENKENEGVKYVRMERRMGKFMRKFQLP 115
Query: 420 TGYEVNDLVSTLSSDGVLTVTAPKRPPPNAAK 515
++ D +S + DGVL VT K PPP K
Sbjct: 116 ENADL-DKISAVCHDGVLKVTVQKLPPPEPKK 146
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 38.3 bits (85), Expect = 0.004
Identities = 26/71 (36%), Positives = 41/71 (57%), Gaps = 8/71 (11%)
Frame = +3
Query: 303 DEITVKASNNTVV-VEG-KHEEKQDEHGFISR------QFTRRYILPTGYEVNDLVSTLS 458
+E+ V+ ++V+ + G +H EK+D++ R QFTRR+ LP ++ D V
Sbjct: 69 EEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKM-DQVKAAM 127
Query: 459 SDGVLTVTAPK 491
+GVLTVT PK
Sbjct: 128 ENGVLTVTVPK 138
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 36.3 bits (80), Expect = 0.017
Identities = 32/95 (33%), Positives = 49/95 (51%), Gaps = 3/95 (3%)
Frame = +3
Query: 216 ELSQAGIFFDDQFTKEKFEVILDDQQFTPDEITVKASNNTVVVEGKHEEKQDEHGFISR- 392
E ++A +F D +K EV ++ + D+ +K S V + EEKQD + R
Sbjct: 54 ETAEAHVFKADLPGMKKEEVKVEIE----DDSVLKISGERHV---EKEEKQDTWHRVERS 106
Query: 393 --QFTRRYILPTGYEVNDLVSTLSSDGVLTVTAPK 491
QF+R++ LP ++ D V +GVLTVT PK
Sbjct: 107 SGQFSRKFKLPENVKM-DQVKASMENGVLTVTVPK 140
>At5g51440.1 68418.m06377 23.5 kDa mitochondrial small heat shock
protein (HSP23.5-M) similar to heat shock 22 kDa
protein, mitochondrial precursor SP:Q96331 from
[Arabidopsis thaliana]; identified in Scharf, K-D., et
al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 210
Score = 35.9 bits (79), Expect = 0.022
Identities = 26/83 (31%), Positives = 41/83 (49%), Gaps = 5/83 (6%)
Frame = +3
Query: 258 KEKFEVI---LDDQQFTPDEITVKASNNTVVV--EGKHEEKQDEHGFISRQFTRRYILPT 422
KEK + + +D + +++ + NT+V+ EG+ EE +D G R+FT R LP
Sbjct: 114 KEKDDALHLRIDMPGLSREDVKLALEQNTLVIRGEGETEEGEDVSGD-GRRFTSRIELPE 172
Query: 423 GYEVNDLVSTLSSDGVLTVTAPK 491
D + +GVL V PK
Sbjct: 173 KVYKTDEIKAEMKNGVLKVVIPK 195
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 35.5 bits (78), Expect = 0.030
Identities = 19/52 (36%), Positives = 34/52 (65%), Gaps = 3/52 (5%)
Frame = +3
Query: 351 KHEEKQDEHGFISR---QFTRRYILPTGYEVNDLVSTLSSDGVLTVTAPKRP 497
++EEK D+ + R +FTRR+ LP ++ ++ +++ +GVL+VT PK P
Sbjct: 94 ENEEKNDKWHRVERSSGKFTRRFRLPENAKMEEIKASM-ENGVLSVTVPKVP 144
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 35.1 bits (77), Expect = 0.039
Identities = 23/73 (31%), Positives = 42/73 (57%), Gaps = 8/73 (10%)
Frame = +3
Query: 303 DEITVKASNNTVV-VEGK----HEEKQDEHGFISR---QFTRRYILPTGYEVNDLVSTLS 458
+E+ V+ + V+ + G+ +EEK D+ + R +F RR+ LP ++ ++ +T+
Sbjct: 75 EEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKMEEVKATME 134
Query: 459 SDGVLTVTAPKRP 497
+ GVLTV PK P
Sbjct: 135 N-GVLTVVVPKAP 146
>At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein
(HSP17.6A-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]
Length = 155
Score = 34.3 bits (75), Expect = 0.068
Identities = 31/95 (32%), Positives = 48/95 (50%), Gaps = 3/95 (3%)
Frame = +3
Query: 216 ELSQAGIFFDDQFTKEKFEVILDDQQFTPDEITVKASNNTVVVEGKHEEKQDEHGFISRQ 395
E ++A +F D +K EV ++ + D+ +K S V + EEKQD + R
Sbjct: 52 ETAEAHVFKADLPGMKKEEVKVEIE----DDSVLKISGERHV---EKEEKQDTWHRVERS 104
Query: 396 ---FTRRYILPTGYEVNDLVSTLSSDGVLTVTAPK 491
F+R++ LP ++ D V +GVLTVT PK
Sbjct: 105 SGGFSRKFRLPENVKM-DQVKASMENGVLTVTVPK 138
>At4g25200.1 68417.m03627 23.6 kDa mitochondrial small heat shock
protein (HSP23.6-M) contains Pfam profile PF00011:
Hsp20/alpha crystallin family
Length = 210
Score = 33.9 bits (74), Expect = 0.090
Identities = 26/84 (30%), Positives = 42/84 (50%), Gaps = 6/84 (7%)
Frame = +3
Query: 258 KEKFEVI---LDDQQFTPDEITVKASNNTVVV--EGKHEEKQDEHGFI-SRQFTRRYILP 419
KEK + + +D + +++ + +T+V+ EGK+EE E G +R+FT R LP
Sbjct: 112 KEKDDALYLRIDMPGLSREDVKLALEQDTLVIRGEGKNEEDGGEEGESGNRRFTSRIGLP 171
Query: 420 TGYEVNDLVSTLSSDGVLTVTAPK 491
D + +GVL V PK
Sbjct: 172 DKIYKIDEIKAEMKNGVLKVVIPK 195
>At1g63230.1 68414.m07147 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 323
Score = 32.7 bits (71), Expect = 0.21
Identities = 18/54 (33%), Positives = 27/54 (50%)
Frame = +3
Query: 285 DQQFTPDEITVKASNNTVVVEGKHEEKQDEHGFISRQFTRRYILPTGYEVNDLV 446
++Q PD +T A N +V EGK E ++ +G RR I PT N ++
Sbjct: 139 ERQINPDVVTFSALINALVKEGKVSEAEEIYG----DMLRRGIFPTTITYNSMI 188
>At3g05680.1 68416.m00634 expressed protein
Length = 2057
Score = 30.7 bits (66), Expect = 0.84
Identities = 16/52 (30%), Positives = 24/52 (46%), Gaps = 1/52 (1%)
Frame = +2
Query: 431 GQRPGQHV-VFRRRAHRHGAETTST*CGERIVPITKTGPAKQPEAASSKPEQ 583
G+ P HV F R G ++ GE +V + PA+ E + KP+Q
Sbjct: 1522 GRPPSIHVDEFMARQRERGQNPSTIVVGEAVVQVKNPTPARDTEKVAGKPKQ 1573
>At2g18220.1 68415.m02123 expressed protein contains Pfam domain
PF03715: Uncharacterised protein family (UPF0120)
Length = 779
Score = 30.3 bits (65), Expect = 1.1
Identities = 21/87 (24%), Positives = 34/87 (39%), Gaps = 4/87 (4%)
Frame = +3
Query: 282 DDQQFTPDEITVKASNNTVVVEGKHEEKQDEHGFISRQFTRRYILPTGYEVNDLV----S 449
D ++ P+E K + K E+KQDE G ++L + E DL
Sbjct: 676 DSKEKEPEEEKTKKKKRKRGGKSKTEKKQDEQGLGEDDVVEDFVLSSDEEEEDLFDIGGD 735
Query: 450 TLSSDGVLTVTAPKRPPPNAAKGSYRS 530
D V + P+ KG+Y++
Sbjct: 736 KDEDDAVDEIADPETKTSKKTKGTYKT 762
>At3g18210.1 68416.m02316 oxidoreductase, 2OG-Fe(II) oxygenase
family protein contains Pfam domain PF03171 2OG-Fe(II)
oxygenase superfamily
Length = 394
Score = 29.9 bits (64), Expect = 1.5
Identities = 15/27 (55%), Positives = 18/27 (66%)
Frame = +2
Query: 440 PGQHVVFRRRAHRHGAETTST*CGERI 520
PGQ V+ R R HRHGA T+ CG R+
Sbjct: 316 PGQAVLHRGR-HRHGARATT--CGHRV 339
>At2g15630.1 68415.m01790 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 627
Score = 29.5 bits (63), Expect = 1.9
Identities = 19/63 (30%), Positives = 27/63 (42%)
Frame = +3
Query: 279 LDDQQFTPDEITVKASNNTVVVEGKHEEKQDEHGFISRQFTRRYILPTGYEVNDLVSTLS 458
+D PD++T + EGK EE ++ G + RR I P N L+S S
Sbjct: 493 MDMMSINPDDVTYNCLMRGLCGEGKFEEARELMG----EMKRRGIKPDHISYNTLISGYS 548
Query: 459 SDG 467
G
Sbjct: 549 KKG 551
>At2g02160.1 68415.m00152 zinc finger (CCCH-type) family protein
contains Pfam domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar)
Length = 669
Score = 29.1 bits (62), Expect = 2.6
Identities = 17/59 (28%), Positives = 27/59 (45%)
Frame = +3
Query: 255 TKEKFEVILDDQQFTPDEITVKASNNTVVVEGKHEEKQDEHGFISRQFTRRYILPTGYE 431
T+EK EV+ + + +E TV +V E +E +DE + + Y GYE
Sbjct: 566 TEEKTEVVSEPKAEVEEEGTVMEEEE-IVGEEVYEGNEDEQAYEGDELNGEYYYEEGYE 623
>At1g73770.1 68414.m08541 hypothetical protein
Length = 191
Score = 29.1 bits (62), Expect = 2.6
Identities = 27/108 (25%), Positives = 51/108 (47%), Gaps = 7/108 (6%)
Frame = +3
Query: 165 IPL-DLALQKFIIQTLEGELSQAGIFFDDQFTKE----KFEVILDD--QQFTPDEITVKA 323
+PL + A+ +F+ + E + S+ G ++ +KE + + LDD + PD
Sbjct: 35 VPLFERAISRFVFFSSESDSSR-GFRTEEVLSKEELKKRIQSFLDDGNEDAIPDLFEALM 93
Query: 324 SNNTVVVEGKHEEKQDEHGFISRQFTRRYILPTGYEVNDLVSTLSSDG 467
+ GKH++ DE R+Y + ++V+D+ S + SDG
Sbjct: 94 IRK---LSGKHDDSDDE----VMDVVRKYPVNDAHKVDDIDSDIESDG 134
>At3g26125.1 68416.m03258 cytochrome P450, putative
Length = 541
Score = 28.7 bits (61), Expect = 3.4
Identities = 16/57 (28%), Positives = 28/57 (49%), Gaps = 1/57 (1%)
Frame = +3
Query: 249 QFTKEKFEVILDDQQFTPDEITVKASNNTVVVEGKHEEKQDEHGF-ISRQFTRRYIL 416
+F KE+F +L+D F D+ + K ++ E H + +H F +R R +L
Sbjct: 116 EFYKERFRDLLEDGIFNADDESWKEQRRIIITE-MHSTRFVDHSFQTTRDLIERKLL 171
>At2g41450.1 68415.m05121 GCN5-related N-acetyltransferase (GNAT)
family protein low similarity to Swift [Xenopus laevis]
GI:14164561; contains Pfam profiles PF00583:
acetyltransferase, GNAT family, PF00533: BRCA1 C
Terminus (BRCT) domain
Length = 991
Score = 28.3 bits (60), Expect = 4.5
Identities = 14/42 (33%), Positives = 20/42 (47%)
Frame = +3
Query: 129 SEEGRSVILHQFIPLDLALQKFIIQTLEGELSQAGIFFDDQF 254
S R +L F+PL LA F++Q G +FDD +
Sbjct: 580 SSYSRKFLLITFLPLSLACLAFLLQWRSGVNDSVTQWFDDNY 621
>At1g24540.1 68414.m03089 cytochrome P450, putative similar to
GB:AAB87111, similar to ESTs dbj|D41610, gb|T20562 and
emb|Z26058
Length = 522
Score = 28.3 bits (60), Expect = 4.5
Identities = 10/32 (31%), Positives = 18/32 (56%)
Frame = -3
Query: 499 GGRFGAVTVSTPSEDNVLTRSLTSYPVGRMYR 404
GG +G +T + +++L + +YP G YR
Sbjct: 85 GGAYGIMTADPANVEHILKTNFKNYPKGAFYR 116
>At5g37670.1 68418.m04537 15.7 kDa class I-related small heat shock
protein-like (HSP15.7-CI) contains Pfam profile: PF00011
Hsp20/alpha crystallin family; identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 137
Score = 27.9 bits (59), Expect = 5.9
Identities = 16/41 (39%), Positives = 21/41 (51%)
Frame = +3
Query: 393 QFTRRYILPTGYEVNDLVSTLSSDGVLTVTAPKRPPPNAAK 515
+F RR LP +V D V +GVLTV PK ++K
Sbjct: 88 EFLRRIELPENVKV-DQVKAYVENGVLTVVVPKDTSSKSSK 127
>At3g44610.1 68416.m04796 protein kinase family protein similar to
viroid symptom modulation protein (protein
kinase)[Lycopersicon esculentum] gi|7672777|gb|AAF66637;
contains protein kinase domain, Pfam:PF00069
Length = 451
Score = 27.9 bits (59), Expect = 5.9
Identities = 14/38 (36%), Positives = 19/38 (50%)
Frame = +1
Query: 424 DTRSTTWSARCLPTACSPSRRRNDLHLMRRKDRTDHEN 537
D R TT S+ +P P+ + RRK +TDH N
Sbjct: 259 DHRQTTSSSCMIPNCIVPAVSCFHPRIRRRKKKTDHRN 296
>At1g13140.1 68414.m01523 cytochrome P450 family protein similar to
Cytochrome P450 86A2 (SP:O23066) [Arabidopsis thaliana];
contains Pfam PF|00067 Cytochrome P450 family
Length = 519
Score = 27.9 bits (59), Expect = 5.9
Identities = 15/48 (31%), Positives = 23/48 (47%)
Frame = +3
Query: 252 FTKEKFEVILDDQQFTPDEITVKASNNTVVVEGKHEEKQDEHGFISRQ 395
F KE+F +L+D F D + K ++ E H + EH F + Q
Sbjct: 117 FFKERFNDLLEDGIFNADAESWKEQRRIIITE-MHSTRFVEHSFQTTQ 163
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,787,419
Number of Sequences: 28952
Number of extensions: 255486
Number of successful extensions: 973
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 929
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 969
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1295224128
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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