BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0385 (616 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g62670.1 68418.m07865 ATPase, plasma membrane-type, putative ... 31 0.61 At5g09620.1 68418.m01113 octicosapeptide/Phox/Bem1p (PB1) domain... 30 1.4 At4g30180.1 68417.m04291 expressed protein 29 1.9 At4g13800.1 68417.m02139 permease-related contains 9 predicted t... 29 1.9 At3g47950.1 68416.m05228 ATPase, plasma membrane-type, putative ... 29 1.9 At5g57180.2 68418.m07143 expressed protein ; supporting cDNA gi|... 29 3.2 At5g57180.1 68418.m07142 expressed protein ; supporting cDNA gi|... 29 3.2 At5g22890.1 68418.m02677 zinc finger (C2H2 type) family protein ... 29 3.2 At5g67370.1 68418.m08495 expressed protein similar to unknown pr... 27 7.5 At5g10380.1 68418.m01204 zinc finger (C3HC4-type RING finger) fa... 27 7.5 At1g11520.1 68414.m01323 pliceosome associated protein-related c... 27 7.5 At3g42640.1 68416.m04431 ATPase, plasma membrane-type, putative ... 27 9.9 At1g60220.1 68414.m06782 Ulp1 protease family protein contains P... 27 9.9 >At5g62670.1 68418.m07865 ATPase, plasma membrane-type, putative / proton pump, putative strong similarity to P-type H(+)-transporting ATPase from Nicotiana plumbaginifolia [SP|Q08435, SP|Q08436], Lycopersicon esculentum [GI:5901757, SP|P22180], Solanum tuberosum [GI:435003]; contains InterPro accession IPR001757: ATPase, E1-E2 type Length = 956 Score = 31.1 bits (67), Expect = 0.61 Identities = 24/95 (25%), Positives = 45/95 (47%) Frame = -3 Query: 512 VVLPLARLMRWMLLVKPTWLWLMMATSLWRVSML*LPCIWTLEVVTLVPPEKMLAVPRAR 333 V LA+L+ ++ V W + + W + + IW +V +P + + + R Sbjct: 797 VAFILAQLVATLIAVYANWSFAAIEGIGWGWAGV----IWLYNIVFYIPLDIIKFLIRYA 852 Query: 332 VN*RAWXLMVLQQ*AAVTERVLVSKELRRWKFCHS 228 ++ RAW L V++Q A T + KE R ++ H+ Sbjct: 853 LSGRAWDL-VIEQRVAFTRQKDFGKEQRELQWAHA 886 >At5g09620.1 68418.m01113 octicosapeptide/Phox/Bem1p (PB1) domain-containing protein predicted proteins, Arabidopsis thaliana and Drosophila melanogaster contains Pfam profile PF00564: PB1 domain Length = 531 Score = 29.9 bits (64), Expect = 1.4 Identities = 15/50 (30%), Positives = 23/50 (46%) Frame = +1 Query: 427 HNDVAIINHNHVGFTNNIQRINLASGSTTLLVLGPGLPASEGPPMLLREP 576 H D + N G+ + + G + +GP + S GPPM L+EP Sbjct: 415 HPDAYMEQQNQPGYNVVQPQPTFSGGPQVMTSVGPQVMTSVGPPMGLQEP 464 >At4g30180.1 68417.m04291 expressed protein Length = 158 Score = 29.5 bits (63), Expect = 1.9 Identities = 16/40 (40%), Positives = 22/40 (55%) Frame = +3 Query: 18 SSQQY*WRWRPWSWQKSPSNSTTTSRSVSPGPRVLDAPRK 137 S+Q++ W S SN TTT+ S S G R+L+ P K Sbjct: 62 SAQEFAWSRFLLQKLSSSSNPTTTTSSSSDGIRILERPDK 101 >At4g13800.1 68417.m02139 permease-related contains 9 predicted transmembrane domains; contains Pfam PF05653: Protein of unknown function (DUF803); identified as COG0697, Permeases of the drug/metabolite transporter (DMT) superfamily Length = 336 Score = 29.5 bits (63), Expect = 1.9 Identities = 14/34 (41%), Positives = 21/34 (61%) Frame = -1 Query: 217 HKSSKMGVSTSVGGRTTHNPGTVEVSGFLGASKT 116 HK+ MG STS+ G T+H+P V G+S++ Sbjct: 294 HKTKDMGNSTSLRGSTSHSPRDTPVFINSGSSRS 327 >At3g47950.1 68416.m05228 ATPase, plasma membrane-type, putative / proton pump, putative strong similarity to P-type H(+)-transporting ATPase from Nicotiana plumbaginifolia [SP|Q08435, SP|Q08436], Lycopersicon esculentum [GI:5901757, SP|P22180], Solanum tuberosum [GI:435003]; contains InterPro accession IPR001757: ATPase, E1-E2 type Length = 960 Score = 29.5 bits (63), Expect = 1.9 Identities = 22/91 (24%), Positives = 43/91 (47%) Frame = -3 Query: 500 LARLMRWMLLVKPTWLWLMMATSLWRVSML*LPCIWTLEVVTLVPPEKMLAVPRARVN*R 321 LA+L+ ++ V W + + W + + IW ++ +P + + R ++ R Sbjct: 805 LAQLVATLIAVYANWSFAAIEGIGWGWAGV----IWLYNIIFYIPLDFIKFFIRYALSGR 860 Query: 320 AWXLMVLQQ*AAVTERVLVSKELRRWKFCHS 228 AW L V++Q A T + KE R ++ H+ Sbjct: 861 AWDL-VIEQRVAFTRQKDFGKEQRELQWAHA 890 >At5g57180.2 68418.m07143 expressed protein ; supporting cDNA gi|13991645|gb|AF359387.1|AF359387 Length = 435 Score = 28.7 bits (61), Expect = 3.2 Identities = 11/25 (44%), Positives = 17/25 (68%) Frame = +3 Query: 69 PSNSTTTSRSVSPGPRVLDAPRKPL 143 PS+STTT+R+ SP + ++ PL Sbjct: 27 PSSSTTTTRATSPSSTISESSNSPL 51 >At5g57180.1 68418.m07142 expressed protein ; supporting cDNA gi|13991645|gb|AF359387.1|AF359387 Length = 424 Score = 28.7 bits (61), Expect = 3.2 Identities = 11/25 (44%), Positives = 17/25 (68%) Frame = +3 Query: 69 PSNSTTTSRSVSPGPRVLDAPRKPL 143 PS+STTT+R+ SP + ++ PL Sbjct: 27 PSSSTTTTRATSPSSTISESSNSPL 51 >At5g22890.1 68418.m02677 zinc finger (C2H2 type) family protein contains Pfam domain PF00096: Zinc finger, C2H2 type Length = 373 Score = 28.7 bits (61), Expect = 3.2 Identities = 18/58 (31%), Positives = 27/58 (46%) Frame = +1 Query: 361 SGGTRVTTSNVQMHGSYNMDTLHNDVAIINHNHVGFTNNIQRINLASGSTTLLVLGPG 534 +G + +V + YN+ TLH V I + + IN +SGST+L V G Sbjct: 42 TGTKSMEDDDVSLSLLYNLSTLHEKVHQIQSLVSFYMVSTNNINQSSGSTSLAVANIG 99 >At5g67370.1 68418.m08495 expressed protein similar to unknown protein (gb|AAC18972.1) Length = 327 Score = 27.5 bits (58), Expect = 7.5 Identities = 9/23 (39%), Positives = 12/23 (52%) Frame = +1 Query: 154 YQDCGWFCRQRWCSPPSCWTCDR 222 Y++ GW+ Q W PP DR Sbjct: 185 YEESGWYDGQMWVKPPEVLARDR 207 >At5g10380.1 68418.m01204 zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) Length = 301 Score = 27.5 bits (58), Expect = 7.5 Identities = 12/26 (46%), Positives = 12/26 (46%) Frame = +2 Query: 530 LGCRLRKDLRCCFGSQQPTKTPSEPP 607 L C KDLR CF P P PP Sbjct: 10 LSCLQFKDLRFCFRQYPPPPPPPPPP 35 >At1g11520.1 68414.m01323 pliceosome associated protein-related contains similarity to spliceosome associated protein SAP 145 GI:1173904 from [Homo sapiens] Length = 196 Score = 27.5 bits (58), Expect = 7.5 Identities = 16/42 (38%), Positives = 24/42 (57%) Frame = -2 Query: 600 SLGVFVGCWLPKQHRRSFRSRQPRPKYQQSCASTG*VDALDV 475 S+ V LPK+ R R R+ R + +++ AS VDA+DV Sbjct: 48 SIVAHVDSVLPKKSREIDRRRRRRKRKKKNKASQADVDAMDV 89 >At3g42640.1 68416.m04431 ATPase, plasma membrane-type, putative / proton pump, putative strong similarity to P-type H+-ATPase from [Lycopersicon esculentum] GI:1621440, [Solanum tuberosum] GI:435001, SP|Q03194 {Nicotiana plumbaginifolia}; contains InterPro accession IPR001757: ATPase, E1-E2 type Length = 948 Score = 27.1 bits (57), Expect = 9.9 Identities = 11/42 (26%), Positives = 23/42 (54%) Frame = -3 Query: 398 IWTLEVVTLVPPEKMLAVPRARVN*RAWXLMVLQQ*AAVTER 273 IW ++T +P + + + R + +AW M+ Q+ A T++ Sbjct: 826 IWVYSIITYIPLDILKFIIRYALTGKAWDNMINQKTAFTTKK 867 >At1g60220.1 68414.m06782 Ulp1 protease family protein contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain Length = 604 Score = 27.1 bits (57), Expect = 9.9 Identities = 9/13 (69%), Positives = 11/13 (84%) Frame = +3 Query: 45 RPWSWQKSPSNST 83 R WSW+KSPS S+ Sbjct: 302 REWSWEKSPSQSS 314 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,069,209 Number of Sequences: 28952 Number of extensions: 210542 Number of successful extensions: 797 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 775 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 797 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1236350304 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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