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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0378
         (744 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical...    60   1e-09
At5g55160.1 68418.m06877 small ubiquitin-like modifier 2 (SUMO) ...    60   2e-09
At5g55170.1 68418.m06878 small ubiquitin-like modifier 3 (SUMO) ...    50   2e-06
At2g32765.1 68415.m04009 small ubiquitin-like modifier 5 (SUMO) ...    40   0.001
At1g68185.1 68414.m07789 ubiquitin-related similar to ubiquitin-...    33   0.20 
At5g48710.1 68418.m06029 ubiquitin-related similar to SP|O13351 ...    30   1.4  
At1g67180.1 68414.m07642 zinc finger (C3HC4-type RING finger) fa...    28   5.7  

>At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical to
           Ubiquitin-like protein SMT3 SP:P55852 from[Arabidopsis
           thaliana]; identical to cDNA SMT3 protein GI:1707371
          Length = 100

 Score = 60.5 bits (140), Expect = 1e-09
 Identities = 30/59 (50%), Positives = 38/59 (64%), Gaps = 1/59 (1%)
 Frame = +2

Query: 98  DEKKGEN-EHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFNLMGNQ 271
           D+K G+   HINLKV GQD   V F+IK+ T L+KLMNAYCDR  + M  + F   G +
Sbjct: 8   DKKPGDGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDMNSIAFLFDGRR 66



 Score = 38.7 bits (86), Expect = 0.004
 Identities = 18/43 (41%), Positives = 26/43 (60%), Gaps = 1/43 (2%)
 Frame = +1

Query: 226 RSINAGS-AIQFDGQPINENDTPTSLEMEEGDTIEVYQQQTGG 351
           +S++  S A  FDG+ +    TP  L+ME+GD I+    QTGG
Sbjct: 51  QSVDMNSIAFLFDGRRLRAEQTPDELDMEDGDEIDAMLHQTGG 93


>At5g55160.1 68418.m06877 small ubiquitin-like modifier 2 (SUMO)
           similar to ubiquitin-like protein SMT3 SP:P55852 from
           [Arabidopsis thaliana]; identical to cDNA small
           ubiquitin-like modifier 2 (SUMO)  GI:22652843; contains
           Pfam profile PF00240: Ubiquitin family
          Length = 103

 Score = 60.1 bits (139), Expect = 2e-09
 Identities = 28/58 (48%), Positives = 36/58 (62%)
 Frame = +2

Query: 98  DEKKGENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFNLMGNQ 271
           D+K  +  HINLKV GQD   V F+IK+ T L+KLMNAYCDR  +    + F   G +
Sbjct: 8   DKKPDQGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDFNSIAFLFDGRR 65



 Score = 39.5 bits (88), Expect = 0.002
 Identities = 17/35 (48%), Positives = 21/35 (60%)
 Frame = +1

Query: 247 AIQFDGQPINENDTPTSLEMEEGDTIEVYQQQTGG 351
           A  FDG+ +    TP  LEME+GD I+    QTGG
Sbjct: 58  AFLFDGRRLRAEQTPDELEMEDGDEIDAMLHQTGG 92


>At5g55170.1 68418.m06878 small ubiquitin-like modifier 3 (SUMO)
           similar to SP|O13351 Ubiquitin-like protein smt3/pmt3
           {Schizosaccharomyces pombe}; identical to cDNA small
           ubiquitin-like modifier 3 (SUMO) GI:22652845
          Length = 111

 Score = 49.6 bits (113), Expect = 2e-06
 Identities = 24/51 (47%), Positives = 28/51 (54%)
 Frame = +2

Query: 113 ENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFNLMG 265
           +  H+ LKV  QD   V FK KK  PL+KLM  YCDR GL +    F   G
Sbjct: 14  QEAHVILKVKSQDGDEVLFKNKKSAPLKKLMYVYCDRRGLKLDAFAFIFNG 64



 Score = 32.7 bits (71), Expect = 0.26
 Identities = 14/36 (38%), Positives = 22/36 (61%)
 Frame = +1

Query: 247 AIQFDGQPINENDTPTSLEMEEGDTIEVYQQQTGGV 354
           A  F+G  I   +TP  L+ME+GD I+  +  +GG+
Sbjct: 59  AFIFNGARIGGLETPDELDMEDGDVIDACRAMSGGL 94


>At2g32765.1 68415.m04009 small ubiquitin-like modifier 5 (SUMO)
           similar to ubiquitin-like protein SMT3 SP:P55852
           [Arabidopsis thaliana]; contains INTERPRO:IPR000626
           ubiquitin domain; contains Pfam profile PF00240:
           Ubiquitin family; contains Pfam profile PF00240:
           Ubiquitin family;  identical to cDNA small
           ubiquitin-like modifier 5 (SUMO) mRNA GI:22652847
          Length = 108

 Score = 40.3 bits (90), Expect = 0.001
 Identities = 21/64 (32%), Positives = 31/64 (48%)
 Frame = +2

Query: 80  INLKMADEKKGENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFNL 259
           ++ K    +   +  + LKV  Q  A   +KI  H  L+KLM+AYC +  L    VRF  
Sbjct: 13  VSKKSRSPETSPHMKVTLKVKNQQGAEDLYKIGTHAHLKKLMSAYCTKRNLDYSSVRFVY 72

Query: 260 MGNQ 271
            G +
Sbjct: 73  NGRE 76


>At1g68185.1 68414.m07789 ubiquitin-related similar to
           ubiquitin-like protein smt3/pmt3 SP:O13351 from [Fission
           yeast]
          Length = 215

 Score = 33.1 bits (72), Expect = 0.20
 Identities = 14/30 (46%), Positives = 21/30 (70%)
 Frame = +1

Query: 256 FDGQPINENDTPTSLEMEEGDTIEVYQQQT 345
           FDG  I+ + TP+ L ME+ D IEV+ ++T
Sbjct: 186 FDGDKIDPSTTPSELGMEDHDMIEVHTKKT 215


>At5g48710.1 68418.m06029 ubiquitin-related similar to SP|O13351
           Ubiquitin-like protein smt3/pmt3 {Schizosaccharomyces
           pombe}
          Length = 114

 Score = 30.3 bits (65), Expect = 1.4
 Identities = 14/32 (43%), Positives = 17/32 (53%)
 Frame = +1

Query: 256 FDGQPINENDTPTSLEMEEGDTIEVYQQQTGG 351
           FDG  I E  TP  LE ++GD I+    Q  G
Sbjct: 73  FDGSRIREYHTPDELERKDGDEIDAMLCQQSG 104


>At1g67180.1 68414.m07642 zinc finger (C3HC4-type RING finger)
           family protein / BRCT domain-containing protein contains
           Pfam domains PF00533: BRCA1 C Terminus (BRCT) domain and
           PF00097: Zinc finger, C3HC4 type (RING finger)
          Length = 453

 Score = 28.3 bits (60), Expect = 5.7
 Identities = 15/44 (34%), Positives = 19/44 (43%)
 Frame = -1

Query: 633 DVSTKHITMELLKLPTAFGILIIYLTTSYLRRIMLCVARLCYMC 502
           D ST+  T    K P     +I +   S  R I+ C  R CY C
Sbjct: 281 DESTREETEATEKAPAQVSCIICWTEFSSSRGILPCGHRFCYSC 324


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,878,271
Number of Sequences: 28952
Number of extensions: 290198
Number of successful extensions: 620
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 605
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 620
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1643603136
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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