BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0378 (744 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical... 60 1e-09 At5g55160.1 68418.m06877 small ubiquitin-like modifier 2 (SUMO) ... 60 2e-09 At5g55170.1 68418.m06878 small ubiquitin-like modifier 3 (SUMO) ... 50 2e-06 At2g32765.1 68415.m04009 small ubiquitin-like modifier 5 (SUMO) ... 40 0.001 At1g68185.1 68414.m07789 ubiquitin-related similar to ubiquitin-... 33 0.20 At5g48710.1 68418.m06029 ubiquitin-related similar to SP|O13351 ... 30 1.4 At1g67180.1 68414.m07642 zinc finger (C3HC4-type RING finger) fa... 28 5.7 >At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical to Ubiquitin-like protein SMT3 SP:P55852 from[Arabidopsis thaliana]; identical to cDNA SMT3 protein GI:1707371 Length = 100 Score = 60.5 bits (140), Expect = 1e-09 Identities = 30/59 (50%), Positives = 38/59 (64%), Gaps = 1/59 (1%) Frame = +2 Query: 98 DEKKGEN-EHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFNLMGNQ 271 D+K G+ HINLKV GQD V F+IK+ T L+KLMNAYCDR + M + F G + Sbjct: 8 DKKPGDGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDMNSIAFLFDGRR 66 Score = 38.7 bits (86), Expect = 0.004 Identities = 18/43 (41%), Positives = 26/43 (60%), Gaps = 1/43 (2%) Frame = +1 Query: 226 RSINAGS-AIQFDGQPINENDTPTSLEMEEGDTIEVYQQQTGG 351 +S++ S A FDG+ + TP L+ME+GD I+ QTGG Sbjct: 51 QSVDMNSIAFLFDGRRLRAEQTPDELDMEDGDEIDAMLHQTGG 93 >At5g55160.1 68418.m06877 small ubiquitin-like modifier 2 (SUMO) similar to ubiquitin-like protein SMT3 SP:P55852 from [Arabidopsis thaliana]; identical to cDNA small ubiquitin-like modifier 2 (SUMO) GI:22652843; contains Pfam profile PF00240: Ubiquitin family Length = 103 Score = 60.1 bits (139), Expect = 2e-09 Identities = 28/58 (48%), Positives = 36/58 (62%) Frame = +2 Query: 98 DEKKGENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFNLMGNQ 271 D+K + HINLKV GQD V F+IK+ T L+KLMNAYCDR + + F G + Sbjct: 8 DKKPDQGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDFNSIAFLFDGRR 65 Score = 39.5 bits (88), Expect = 0.002 Identities = 17/35 (48%), Positives = 21/35 (60%) Frame = +1 Query: 247 AIQFDGQPINENDTPTSLEMEEGDTIEVYQQQTGG 351 A FDG+ + TP LEME+GD I+ QTGG Sbjct: 58 AFLFDGRRLRAEQTPDELEMEDGDEIDAMLHQTGG 92 >At5g55170.1 68418.m06878 small ubiquitin-like modifier 3 (SUMO) similar to SP|O13351 Ubiquitin-like protein smt3/pmt3 {Schizosaccharomyces pombe}; identical to cDNA small ubiquitin-like modifier 3 (SUMO) GI:22652845 Length = 111 Score = 49.6 bits (113), Expect = 2e-06 Identities = 24/51 (47%), Positives = 28/51 (54%) Frame = +2 Query: 113 ENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFNLMG 265 + H+ LKV QD V FK KK PL+KLM YCDR GL + F G Sbjct: 14 QEAHVILKVKSQDGDEVLFKNKKSAPLKKLMYVYCDRRGLKLDAFAFIFNG 64 Score = 32.7 bits (71), Expect = 0.26 Identities = 14/36 (38%), Positives = 22/36 (61%) Frame = +1 Query: 247 AIQFDGQPINENDTPTSLEMEEGDTIEVYQQQTGGV 354 A F+G I +TP L+ME+GD I+ + +GG+ Sbjct: 59 AFIFNGARIGGLETPDELDMEDGDVIDACRAMSGGL 94 >At2g32765.1 68415.m04009 small ubiquitin-like modifier 5 (SUMO) similar to ubiquitin-like protein SMT3 SP:P55852 [Arabidopsis thaliana]; contains INTERPRO:IPR000626 ubiquitin domain; contains Pfam profile PF00240: Ubiquitin family; contains Pfam profile PF00240: Ubiquitin family; identical to cDNA small ubiquitin-like modifier 5 (SUMO) mRNA GI:22652847 Length = 108 Score = 40.3 bits (90), Expect = 0.001 Identities = 21/64 (32%), Positives = 31/64 (48%) Frame = +2 Query: 80 INLKMADEKKGENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFNL 259 ++ K + + + LKV Q A +KI H L+KLM+AYC + L VRF Sbjct: 13 VSKKSRSPETSPHMKVTLKVKNQQGAEDLYKIGTHAHLKKLMSAYCTKRNLDYSSVRFVY 72 Query: 260 MGNQ 271 G + Sbjct: 73 NGRE 76 >At1g68185.1 68414.m07789 ubiquitin-related similar to ubiquitin-like protein smt3/pmt3 SP:O13351 from [Fission yeast] Length = 215 Score = 33.1 bits (72), Expect = 0.20 Identities = 14/30 (46%), Positives = 21/30 (70%) Frame = +1 Query: 256 FDGQPINENDTPTSLEMEEGDTIEVYQQQT 345 FDG I+ + TP+ L ME+ D IEV+ ++T Sbjct: 186 FDGDKIDPSTTPSELGMEDHDMIEVHTKKT 215 >At5g48710.1 68418.m06029 ubiquitin-related similar to SP|O13351 Ubiquitin-like protein smt3/pmt3 {Schizosaccharomyces pombe} Length = 114 Score = 30.3 bits (65), Expect = 1.4 Identities = 14/32 (43%), Positives = 17/32 (53%) Frame = +1 Query: 256 FDGQPINENDTPTSLEMEEGDTIEVYQQQTGG 351 FDG I E TP LE ++GD I+ Q G Sbjct: 73 FDGSRIREYHTPDELERKDGDEIDAMLCQQSG 104 >At1g67180.1 68414.m07642 zinc finger (C3HC4-type RING finger) family protein / BRCT domain-containing protein contains Pfam domains PF00533: BRCA1 C Terminus (BRCT) domain and PF00097: Zinc finger, C3HC4 type (RING finger) Length = 453 Score = 28.3 bits (60), Expect = 5.7 Identities = 15/44 (34%), Positives = 19/44 (43%) Frame = -1 Query: 633 DVSTKHITMELLKLPTAFGILIIYLTTSYLRRIMLCVARLCYMC 502 D ST+ T K P +I + S R I+ C R CY C Sbjct: 281 DESTREETEATEKAPAQVSCIICWTEFSSSRGILPCGHRFCYSC 324 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,878,271 Number of Sequences: 28952 Number of extensions: 290198 Number of successful extensions: 620 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 605 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 620 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1643603136 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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