Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0374
(718 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 142 4e-35
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 142 4e-35
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 142 4e-35
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 48 1e-06
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 40 4e-04
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 40 5e-04
SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosacc... 29 0.66
SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr 1|||Ma... 28 1.2
SPCC417.10 |||membrane transporter|Schizosaccharomyces pombe|chr... 27 2.0
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 3.5
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 8.2
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 142 bits (344), Expect = 4e-35
Identities = 64/84 (76%), Positives = 72/84 (85%)
Frame = +1
Query: 256 RVETGVLKPGTIVVFAPANITTEVKSVNMHHETLQEAVPGDNVGFNVKNVSVKELRRGYV 435
RVETGV+KPG IV FAPA +TTEVKSV MHHE+L +PGDNVGFNVKNVSVK++RRG V
Sbjct: 264 RVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNV 323
Query: 436 AGDSKNNPPKGAADFTAQVIVLNH 507
GDSKN+PP G A FTAQVI+LNH
Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNH 347
Score = 142 bits (343), Expect = 6e-35
Identities = 59/88 (67%), Positives = 73/88 (82%)
Frame = +2
Query: 2 IGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPAR 181
+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G GK L+EA+D+I PPAR
Sbjct: 179 VGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPAR 238
Query: 182 PTDKPLRLPLQDVYKIGGIGTMPVAELK 265
PTDKPLRLPLQDVYKIGGIGT+PV ++
Sbjct: 239 PTDKPLRLPLQDVYKIGGIGTVPVGRVE 266
Score = 115 bits (277), Expect = 6e-27
Identities = 52/93 (55%), Positives = 65/93 (69%)
Frame = +3
Query: 435 CW*LQKQPT*GCCRFYSSSHCA*PSGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGK 614
C + P GC F + GQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK
Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGK 383
Query: 615 STEVNPKSIKSGDAAIVNLVPSKPLCVESFQEF 713
E +PK +KSGDA I +VPSKP+CVE+F ++
Sbjct: 384 KIEESPKFVKSGDACIAKMVPSKPMCVEAFTDY 416
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 142 bits (344), Expect = 4e-35
Identities = 64/84 (76%), Positives = 72/84 (85%)
Frame = +1
Query: 256 RVETGVLKPGTIVVFAPANITTEVKSVNMHHETLQEAVPGDNVGFNVKNVSVKELRRGYV 435
RVETGV+KPG IV FAPA +TTEVKSV MHHE+L +PGDNVGFNVKNVSVK++RRG V
Sbjct: 264 RVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNV 323
Query: 436 AGDSKNNPPKGAADFTAQVIVLNH 507
GDSKN+PP G A FTAQVI+LNH
Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNH 347
Score = 142 bits (343), Expect = 6e-35
Identities = 59/88 (67%), Positives = 73/88 (82%)
Frame = +2
Query: 2 IGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPAR 181
+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G GK L+EA+D+I PPAR
Sbjct: 179 VGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPAR 238
Query: 182 PTDKPLRLPLQDVYKIGGIGTMPVAELK 265
PTDKPLRLPLQDVYKIGGIGT+PV ++
Sbjct: 239 PTDKPLRLPLQDVYKIGGIGTVPVGRVE 266
Score = 115 bits (277), Expect = 6e-27
Identities = 52/93 (55%), Positives = 65/93 (69%)
Frame = +3
Query: 435 CW*LQKQPT*GCCRFYSSSHCA*PSGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGK 614
C + P GC F + GQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK
Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGK 383
Query: 615 STEVNPKSIKSGDAAIVNLVPSKPLCVESFQEF 713
E +PK +KSGDA I +VPSKP+CVE+F ++
Sbjct: 384 KIEESPKFVKSGDACIAKMVPSKPMCVEAFTDY 416
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 142 bits (344), Expect = 4e-35
Identities = 64/84 (76%), Positives = 72/84 (85%)
Frame = +1
Query: 256 RVETGVLKPGTIVVFAPANITTEVKSVNMHHETLQEAVPGDNVGFNVKNVSVKELRRGYV 435
RVETGV+KPG IV FAPA +TTEVKSV MHHE+L +PGDNVGFNVKNVSVK++RRG V
Sbjct: 264 RVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNV 323
Query: 436 AGDSKNNPPKGAADFTAQVIVLNH 507
GDSKN+PP G A FTAQVI+LNH
Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNH 347
Score = 142 bits (343), Expect = 6e-35
Identities = 59/88 (67%), Positives = 73/88 (82%)
Frame = +2
Query: 2 IGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPAR 181
+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G GK L+EA+D+I PPAR
Sbjct: 179 VGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPAR 238
Query: 182 PTDKPLRLPLQDVYKIGGIGTMPVAELK 265
PTDKPLRLPLQDVYKIGGIGT+PV ++
Sbjct: 239 PTDKPLRLPLQDVYKIGGIGTVPVGRVE 266
Score = 115 bits (277), Expect = 6e-27
Identities = 52/93 (55%), Positives = 65/93 (69%)
Frame = +3
Query: 435 CW*LQKQPT*GCCRFYSSSHCA*PSGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGK 614
C + P GC F + GQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK
Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGK 383
Query: 615 STEVNPKSIKSGDAAIVNLVPSKPLCVESFQEF 713
E +PK +KSGDA I +VPSKP+CVE+F ++
Sbjct: 384 KIEESPKFVKSGDACIAKMVPSKPMCVEAFTDY 416
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 48.4 bits (110), Expect = 1e-06
Identities = 27/77 (35%), Positives = 41/77 (53%), Gaps = 2/77 (2%)
Frame = +2
Query: 2 IGYNPAAVAFVPISGWHGDNMLEPSTK--MPWFKGWQVERKEGKADGKCLIEALDAILPP 175
+G+ + V FVPIS G N+++ + W+KG L+ ALD ++PP
Sbjct: 350 VGFKTSNVHFVPISAISGTNLIQKDSSDLYKWYKG------------PTLLSALDQLVPP 397
Query: 176 ARPTDKPLRLPLQDVYK 226
+P KPLRL + DVY+
Sbjct: 398 EKPYRKPLRLSIDDVYR 414
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 39.9 bits (89), Expect = 4e-04
Identities = 21/62 (33%), Positives = 35/62 (56%), Gaps = 2/62 (3%)
Frame = +1
Query: 256 RVETGVLKPGTIV--VFAPANITTEVKSVNMHHETLQEAVPGDNVGFNVKNVSVKELRRG 429
RVE G LK G + V +++ T V + M + L AV GDN G ++++ ++L+RG
Sbjct: 273 RVERGTLKKGAEIEIVGYGSHLKTTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRG 332
Query: 430 YV 435
+
Sbjct: 333 MI 334
Score = 33.1 bits (72), Expect = 0.041
Identities = 15/36 (41%), Positives = 24/36 (66%), Gaps = 1/36 (2%)
Frame = +2
Query: 143 LIEALDAILP-PARPTDKPLRLPLQDVYKIGGIGTM 247
L+EA+D+ + P R TD P + ++DV+ I G GT+
Sbjct: 234 LMEAVDSYITLPERKTDVPFLMAIEDVFSISGRGTV 269
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 39.5 bits (88), Expect = 5e-04
Identities = 19/66 (28%), Positives = 35/66 (53%)
Frame = +3
Query: 516 ISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCV 695
++ GY+ V+ HTA FA++ K+D +T + ++ P G I L P+C+
Sbjct: 573 LTTGYSCVMHIHTAVEEVSFAKLLHKLD-KTNRKSKKPPMFATKGMKIIAELETQTPVCM 631
Query: 696 ESFQEF 713
E F+++
Sbjct: 632 ERFEDY 637
>SPBC21.01 |mis17|SPBC776.19|kinetochore protein
Mis17|Schizosaccharomyces pombe|chr 2|||Manual
Length = 441
Score = 29.1 bits (62), Expect = 0.66
Identities = 16/66 (24%), Positives = 30/66 (45%)
Frame = +1
Query: 220 IQNRWYWYHARRRVETGVLKPGTIVVFAPANITTEVKSVNMHHETLQEAVPGDNVGFNVK 399
IQ +H R +ET +I+ +P N + S++ ++ QE PG+ G
Sbjct: 139 IQRESIPFHKRDNIETSDAYSSSILENSPPNKVQRLSSLDSSQDSFQEEHPGNVTGTTFS 198
Query: 400 NVSVKE 417
+ + +E
Sbjct: 199 SQAPEE 204
>SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 835
Score = 28.3 bits (60), Expect = 1.2
Identities = 15/46 (32%), Positives = 19/46 (41%)
Frame = -2
Query: 183 GRAGGRMASRASMRHFPSALPSLRSTCHPLNQGILVEGSNMLSPCH 46
G GG S+ P+ LPS + P L E +ML CH
Sbjct: 545 GAGGGHAPDIISLVQNPNILPSSTNPTRPFTTNTLDEELDMLMVCH 590
>SPCC417.10 |||membrane transporter|Schizosaccharomyces pombe|chr
3|||Manual
Length = 508
Score = 27.5 bits (58), Expect = 2.0
Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 2/57 (3%)
Frame = -3
Query: 332 DLTSVVMLAGAKTTMVP--GFNTPVSTLRRAWYQYHRFCIRLAGEDAGACQWGGQVA 168
D+ + +M + +P G T S L + Y L G AGAC++GG +A
Sbjct: 289 DIRTWIMFVSSVLLNIPNGGIGTFSSLLIKGTMGYDTLQTLLMGLPAGACEFGGLIA 345
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 3.5
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -2
Query: 420 QFLDGHVLYVETYIVSRYSFLESFV 346
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 8.2
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = -1
Query: 661 MAASPDLMDFGLTSVDLPVRRSTFS 587
++ +PDL D L+SVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,406,507
Number of Sequences: 5004
Number of extensions: 75368
Number of successful extensions: 228
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 206
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 227
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 335201398
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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