Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0368
(677 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 269 3e-73
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 268 4e-73
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 268 4e-73
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 142 6e-35
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 138 5e-34
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 73 3e-14
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 39 6e-04
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 34 0.016
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 34 0.022
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 33 0.029
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 32 0.088
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 31 0.20
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 30 0.27
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 30 0.27
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 29 0.47
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 27 1.9
SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|c... 27 3.3
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 26 5.8
SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr 1||... 25 7.6
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 269 bits (659), Expect = 3e-73
Identities = 136/215 (63%), Positives = 160/215 (74%), Gaps = 4/215 (1%)
Frame = +2
Query: 44 MGKEKTHINIVVIGHVDSGKSTTTGHLHLQMWWY*----QTYHREVREGGPGNG*RILQI 211
MGKEK HIN+VVIGHVDSGKSTTTGHL + + + +E E G G+ +
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGS----FKY 56
Query: 212 CLGIGQTKG*A*AWFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 391
+ + K TIDIALWKFET KY VT+IDAPGHRDFIKNMITGTSQADCAVLI+
Sbjct: 57 AWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAVLII 116
Query: 392 AAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMIPLNHHYSEPRFEEIKKEVSS 571
GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKM +S+ RFEEI KE S+
Sbjct: 117 GGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTTG--WSQARFEEIVKETSN 174
Query: 572 YIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWF 676
+IKK+G+NP V FVP+SG+ GDNM+EP+T MPW+
Sbjct: 175 FIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWY 209
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 268 bits (658), Expect = 4e-73
Identities = 135/215 (62%), Positives = 160/215 (74%), Gaps = 4/215 (1%)
Frame = +2
Query: 44 MGKEKTHINIVVIGHVDSGKSTTTGHLHLQMWWY*----QTYHREVREGGPGNG*RILQI 211
MGKEK HIN+VVIGHVDSGKSTTTGHL + + + +E E G G+ +
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGS----FKY 56
Query: 212 CLGIGQTKG*A*AWFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 391
+ + K TIDIALWKFET KY VT+IDAPGHRDFIKNMITGTSQADCA+LI+
Sbjct: 57 AWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILII 116
Query: 392 AAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMIPLNHHYSEPRFEEIKKEVSS 571
GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKM +S+ RFEEI KE S+
Sbjct: 117 GGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTTG--WSQARFEEIVKETSN 174
Query: 572 YIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWF 676
+IKK+G+NP V FVP+SG+ GDNM+EP+T MPW+
Sbjct: 175 FIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWY 209
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 268 bits (658), Expect = 4e-73
Identities = 135/215 (62%), Positives = 160/215 (74%), Gaps = 4/215 (1%)
Frame = +2
Query: 44 MGKEKTHINIVVIGHVDSGKSTTTGHLHLQMWWY*----QTYHREVREGGPGNG*RILQI 211
MGKEK HIN+VVIGHVDSGKSTTTGHL + + + +E E G G+ +
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGS----FKY 56
Query: 212 CLGIGQTKG*A*AWFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 391
+ + K TIDIALWKFET KY VT+IDAPGHRDFIKNMITGTSQADCA+LI+
Sbjct: 57 AWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILII 116
Query: 392 AAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMIPLNHHYSEPRFEEIKKEVSS 571
GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKM +S+ RFEEI KE S+
Sbjct: 117 GGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTTG--WSQARFEEIVKETSN 174
Query: 572 YIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWF 676
+IKK+G+NP V FVP+SG+ GDNM+EP+T MPW+
Sbjct: 175 FIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWY 209
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 142 bits (343), Expect = 6e-35
Identities = 75/211 (35%), Positives = 120/211 (56%), Gaps = 4/211 (1%)
Frame = +2
Query: 56 KTHINIVVIGHVDSGKSTTTGHLHLQMWWY*QTYHREVREGGPGNG*RILQICLGIGQTK 235
K H+NIV IGHVD+GKST G++ + ++ G + + T
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTS 295
Query: 236 G*A*AWFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFE 415
T+++ FET +++DAPGH+ ++ NMI G SQAD VL+++A GEFE
Sbjct: 296 EEREKGKTVEVGRAYFETEHRRFSLLDAPGHKGYVTNMINGASQADIGVLVISARRGEFE 355
Query: 416 AGISKNGQTREHALLAFTLGVKQLIVGVNKMIPLNHHYSEPRFEEIKKEVSSYIKKI-GY 592
AG + GQTREHA+LA T G+ L+V +NKM + +SE R++E ++S +++++ GY
Sbjct: 356 AGFERGGQTREHAVLARTQGINHLVVVINKMDEPSVQWSEERYKECVDKLSMFLRRVAGY 415
Query: 593 NPAA-VAFVPISGWHGDNMLE--PSTKMPWF 676
N V ++P+S + G N+ + S+ PW+
Sbjct: 416 NSKTDVKYMPVSAYTGQNVKDRVDSSVCPWY 446
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 138 bits (335), Expect = 5e-34
Identities = 79/200 (39%), Positives = 113/200 (56%), Gaps = 1/200 (0%)
Frame = +2
Query: 56 KTHINIVVIGHVDSGKSTTTGHLHLQMWWY*QTYHREVREGGPGNG*RILQICLGIGQTK 235
K +++VV GHVDSGKST G + ++ +++ +G + T+
Sbjct: 175 KPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTE 234
Query: 236 G*A*AWFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFE 415
T+D+A FE+ K I DAPGHRDFI MI G S AD AVL+V + FE
Sbjct: 235 EERARGVTMDVASTTFESDKKIYEIGDAPGHRDFISGMIAGASSADFAVLVVDSSQNNFE 294
Query: 416 AGISKNGQTREHALLAFTLGVKQLIVGVNKMIPLNHHYSEPRFEEIKKEVSSY-IKKIGY 592
G +NGQTREHA L LG+ +++V VNK+ ++ +SE RF+EIK VS + IK +G+
Sbjct: 295 RGFLENGQTREHAYLLRALGISEIVVSVNKLDLMS--WSEDRFQEIKNIVSDFLIKMVGF 352
Query: 593 NPAAVAFVPISGWHGDNMLE 652
+ V FVPIS G N+++
Sbjct: 353 KTSNVHFVPISAISGTNLIQ 372
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 73.3 bits (172), Expect = 3e-14
Identities = 41/106 (38%), Positives = 63/106 (59%)
Frame = +2
Query: 257 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 436
TI A ++ET+ + +D PGH D+IKNMITG + D A+++V+A G+
Sbjct: 103 TISSAHVEYETANRHYAHVDCPGHADYIKNMITGAATMDGAIIVVSATDGQMP------- 155
Query: 437 QTREHALLAFTLGVKQLIVGVNKMIPLNHHYSEPRFEEIKKEVSSY 574
QTREH LLA +GVKQ++V +NK+ + E E+++ +S Y
Sbjct: 156 QTREHLLLARQVGVKQIVVYINKVDMVEPDMIELVEMEMRELLSEY 201
Score = 33.5 bits (73), Expect = 0.029
Identities = 13/22 (59%), Positives = 17/22 (77%)
Frame = +2
Query: 50 KEKTHINIVVIGHVDSGKSTTT 115
++K H+NI IGHVD GK+T T
Sbjct: 49 RKKPHVNIGTIGHVDHGKTTLT 70
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 39.1 bits (87), Expect = 6e-04
Identities = 22/93 (23%), Positives = 42/93 (45%)
Frame = +2
Query: 299 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGV 478
+V+ +D PGH + M+ G + D A+L++A QT EH + +
Sbjct: 109 HVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNES------CPQPQTSEHLAAIEIMQL 162
Query: 479 KQLIVGVNKMIPLNHHYSEPRFEEIKKEVSSYI 577
K +I+ NK+ + +E ++ I K + +
Sbjct: 163 KHIIILQNKVDLIRESAAEEHYQSILKFIKGTV 195
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 34.3 bits (75), Expect = 0.016
Identities = 21/57 (36%), Positives = 29/57 (50%)
Frame = +2
Query: 281 FETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREH 451
FE S Y + IID PGH DF + D AVL++ A +G I+ + Q R +
Sbjct: 141 FEKS-YNINIIDTPGHIDFTIEVERALRVLDGAVLVLCAVSGVQSQTITVDRQMRRY 196
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 33.9 bits (74), Expect = 0.022
Identities = 30/113 (26%), Positives = 49/113 (43%)
Frame = +2
Query: 68 NIVVIGHVDSGKSTTTGHLHLQMWWY*QTYHREVREGGPGNG*RILQICLGIGQTKG*A* 247
N+ +I H+D+GK+T T + +++ T H G G ++ L + +G
Sbjct: 30 NVGIIAHIDAGKTTLTEKM---LYYGGFTSHF----GNVDTGDTVMDY-LPAERQRG--- 78
Query: 248 AWFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 406
TI+ A F + +ID PGH DF + + D AV I+ G
Sbjct: 79 --ITINSAAISFTWRNQRINLIDTPGHADFTFEVERSVAVLDGAVAIIDGSAG 129
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 33.5 bits (73), Expect = 0.029
Identities = 15/42 (35%), Positives = 21/42 (50%)
Frame = +2
Query: 281 FETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 406
F+ ++T +D PGH F G + AD VL+VA G
Sbjct: 215 FDKGSKFITFLDTPGHMAFEAMRKRGANIADIVVLVVAGDDG 256
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 31.9 bits (69), Expect = 0.088
Identities = 21/86 (24%), Positives = 37/86 (43%), Gaps = 1/86 (1%)
Frame = +2
Query: 296 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT-REHALLAFTL 472
Y + +ID+PGH DF + + + D A ++V A G I+ Q + + +
Sbjct: 94 YLINLIDSPGHVDFSSEVSSASRLCDGAFVLVDAVEGVCSQTITVLRQAWIDRIKVILVI 153
Query: 473 GVKQLIVGVNKMIPLNHHYSEPRFEE 550
++ K+ P+ HY R E
Sbjct: 154 NKMDRLITELKLSPIEAHYHLLRLVE 179
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 30.7 bits (66), Expect = 0.20
Identities = 12/45 (26%), Positives = 23/45 (51%)
Frame = +2
Query: 272 LWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 406
++ + Y + +ID PGH DF ++ + + +L+V A G
Sbjct: 116 IYYYHGQSYLLNLIDTPGHVDFRAEVMHSLAACEGCILLVDASQG 160
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 30.3 bits (65), Expect = 0.27
Identities = 15/27 (55%), Positives = 16/27 (59%)
Frame = +2
Query: 44 MGKEKTHINIVVIGHVDSGKSTTTGHL 124
MGK N+ VI HVD GKST T L
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSL 39
Score = 27.9 bits (59), Expect = 1.4
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +2
Query: 296 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 391
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 30.3 bits (65), Expect = 0.27
Identities = 15/27 (55%), Positives = 16/27 (59%)
Frame = +2
Query: 44 MGKEKTHINIVVIGHVDSGKSTTTGHL 124
MGK N+ VI HVD GKST T L
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSL 39
Score = 27.9 bits (59), Expect = 1.4
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +2
Query: 296 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 391
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPAC56F8.03 |||translation initiation factor IF2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1079
Score = 29.5 bits (63), Expect = 0.47
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 308 IIDAPGHRDFIKNMITGTSQADCAVLIV 391
IID PGH F GTS + A+L++
Sbjct: 553 IIDTPGHESFTNLRSRGTSLCNIAILVI 580
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 27.5 bits (58), Expect = 1.9
Identities = 24/98 (24%), Positives = 42/98 (42%), Gaps = 4/98 (4%)
Frame = +2
Query: 311 IDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEF--EAGISKNGQTREHALLAFTLGVKQ 484
ID PGH DF+ + + +D VL+V G I K+ + ++ V +
Sbjct: 214 IDTPGHVDFVDEVAAPMAISDGVVLVVDVIEGVMINTTRIIKHAILHDMPIVLVLNKVDR 273
Query: 485 LIVGVNKMIPLNHHYSEPR--FEEIKKEVSSYIKKIGY 592
LI+ + +P N Y + R +E+ + K + Y
Sbjct: 274 LILELR--LPPNDAYHKLRHVIDEVNDNICQISKDLKY 309
>SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 367
Score = 26.6 bits (56), Expect = 3.3
Identities = 10/33 (30%), Positives = 19/33 (57%)
Frame = +3
Query: 519 TTITVSPDLRKSRRKYPHTSRRLATTQLLSLSC 617
T + +PD+ ++PHT +R +T + L + C
Sbjct: 142 TNSSTNPDVLYMSSQHPHTQQRYSTLKRLMVRC 174
>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 690
Score = 25.8 bits (54), Expect = 5.8
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = -3
Query: 252 HAHAQPLVCPIPKHI*RILYPFPGPPSRTSRWY 154
H+H PL P+P +L P P P S WY
Sbjct: 135 HSHHPPLHNPLPVSCQPVLRP-PPVPQVPSHWY 166
>SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 380
Score = 25.4 bits (53), Expect = 7.6
Identities = 12/31 (38%), Positives = 18/31 (58%), Gaps = 2/31 (6%)
Frame = +2
Query: 260 IDIALWKFETSKYYV--TIIDAPGHRDFIKN 346
I+I + E +++ T+ID PG DFI N
Sbjct: 73 IEITKAELEEKNFHLRLTVIDTPGFGDFINN 103
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,830,374
Number of Sequences: 5004
Number of extensions: 58172
Number of successful extensions: 207
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 175
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 190
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 311890690
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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