BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0368 (677 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 316 2e-88 X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 307 8e-86 AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 274 5e-76 EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 270 6e-75 AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 35 6e-04 AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.2 DQ667195-1|ABG75747.1| 469|Apis mellifera cys-loop ligand-gated... 23 2.0 AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 2.7 D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 3.5 DQ325103-1|ABD14117.1| 182|Apis mellifera complementary sex det... 23 3.5 DQ667193-1|ABG75745.1| 510|Apis mellifera cys-loop ligand-gated... 22 4.7 DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monoo... 22 6.2 AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic ac... 21 8.2 >AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha F2 protein. Length = 461 Score = 316 bits (775), Expect = 2e-88 Identities = 162/215 (75%), Positives = 172/215 (80%), Gaps = 4/215 (1%) Frame = +2 Query: 44 MGKEKTHINIVVIGHVDSGKSTTTGHLHLQMWWY*----QTYHREVREGGPGNG*RILQI 211 MGKEK HINIVVIGHVDSGKSTTTGHL + + + +E +E G G+ + Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGS----FKY 56 Query: 212 CLGIGQTKG*A*AWFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 391 + + K TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV Sbjct: 57 AWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 116 Query: 392 AAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMIPLNHHYSEPRFEEIKKEVSS 571 AAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKM YSE RFEEIKKEVSS Sbjct: 117 AAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSS 176 Query: 572 YIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWF 676 YIKKIGYNPAAVAFVPISGWHGDNMLE S+KMPWF Sbjct: 177 YIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWF 211 >X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha protein. Length = 461 Score = 307 bits (753), Expect = 8e-86 Identities = 157/215 (73%), Positives = 170/215 (79%), Gaps = 4/215 (1%) Frame = +2 Query: 44 MGKEKTHINIVVIGHVDSGKSTTTGHLHLQMWWY*----QTYHREVREGGPGNG*RILQI 211 MGKEK HINIVVIGHVDSGKSTTTGHL + + + +E +E G G+ + Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGS----FKY 56 Query: 212 CLGIGQTKG*A*AWFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 391 + + K TIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV Sbjct: 57 AWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 116 Query: 392 AAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMIPLNHHYSEPRFEEIKKEVSS 571 AAG GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKM + YSE RFEEIKKEVSS Sbjct: 117 AAGIGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSS 176 Query: 572 YIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWF 676 YIKKIGYN A+VAFVPISGWHGDNMLEPS K PW+ Sbjct: 177 YIKKIGYNTASVAFVPISGWHGDNMLEPSPKTPWY 211 >AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-alpha protein. Length = 274 Score = 274 bits (672), Expect = 5e-76 Identities = 131/140 (93%), Positives = 132/140 (94%) Frame = +2 Query: 257 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 436 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG Sbjct: 15 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 74 Query: 437 QTREHALLAFTLGVKQLIVGVNKMIPLNHHYSEPRFEEIKKEVSSYIKKIGYNPAAVAFV 616 QTREHALLAFTLGVKQLIVGVNKM YSE RFEEIKKEVSSYIKKIGYNPAAVAFV Sbjct: 75 QTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFV 134 Query: 617 PISGWHGDNMLEPSTKMPWF 676 PISGWHGDNMLE S+KMPWF Sbjct: 135 PISGWHGDNMLEVSSKMPWF 154 Score = 31.9 bits (69), Expect = 0.006 Identities = 13/13 (100%), Positives = 13/13 (100%) Frame = +3 Query: 216 WVLDKLKAERERG 254 WVLDKLKAERERG Sbjct: 1 WVLDKLKAERERG 13 >EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-alpha protein. Length = 172 Score = 270 bits (663), Expect = 6e-75 Identities = 129/138 (93%), Positives = 130/138 (94%) Frame = +2 Query: 263 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 442 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60 Query: 443 REHALLAFTLGVKQLIVGVNKMIPLNHHYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPI 622 REHALLAFTLGVKQLIVGVNKM YSE RFEEIKKEVSSYIKKIGYNPAAVAFVPI Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 120 Query: 623 SGWHGDNMLEPSTKMPWF 676 SGWHGDNMLE S+KMPWF Sbjct: 121 SGWHGDNMLEVSSKMPWF 138 >AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation factor 2 protein. Length = 690 Score = 35.1 bits (77), Expect = 6e-04 Identities = 36/117 (30%), Positives = 52/117 (44%) Frame = +2 Query: 302 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 481 VT +D PGH FI G D VL+VAA G E QT + +A V Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV- 246 Query: 482 QLIVGVNKMIPLNHHYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE 652 +IV +NK+ N + ++E K + I+++G + V IS G N+ E Sbjct: 247 PIIVAINKIDKPNIDIIKVQYELAKHGI--VIEELG---GEIQCVKISALKGINLRE 298 Score = 25.4 bits (53), Expect = 0.50 Identities = 9/18 (50%), Positives = 13/18 (72%) Frame = +2 Query: 56 KTHINIVVIGHVDSGKST 109 K H + ++GHVD GK+T Sbjct: 143 KRHPIVTIMGHVDHGKTT 160 >AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methyltransferase protein. Length = 683 Score = 24.2 bits (50), Expect = 1.2 Identities = 11/15 (73%), Positives = 11/15 (73%) Frame = +2 Query: 65 INIVVIGHVDSGKST 109 INI IGHV GKST Sbjct: 43 INIGTIGHVAHGKST 57 >DQ667195-1|ABG75747.1| 469|Apis mellifera cys-loop ligand-gated ion channel subunit protein. Length = 469 Score = 23.4 bits (48), Expect = 2.0 Identities = 6/22 (27%), Positives = 14/22 (63%) Frame = -2 Query: 568 GYFLLDFLKSGLTVMVVQWNHF 503 G+F++D+ + ++V+ W F Sbjct: 232 GFFMMDYYIPSILIVVISWVSF 253 >AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase protein. Length = 567 Score = 23.0 bits (47), Expect = 2.7 Identities = 13/34 (38%), Positives = 18/34 (52%) Frame = +2 Query: 287 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 388 T KYY D P + FIKN+ ++ +D LI Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327 >D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. Length = 567 Score = 22.6 bits (46), Expect = 3.5 Identities = 12/34 (35%), Positives = 18/34 (52%) Frame = +2 Query: 287 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 388 T KYY D P + FIKN+ ++ +D L+ Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327 >DQ325103-1|ABD14117.1| 182|Apis mellifera complementary sex determiner protein. Length = 182 Score = 22.6 bits (46), Expect = 3.5 Identities = 8/36 (22%), Positives = 19/36 (52%) Frame = +2 Query: 515 LNHHYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPI 622 L+++Y+ + ++Y KK+ YN + +P+ Sbjct: 85 LSNNYNYSNYNNYNNNYNNYNKKLYYNINYIEQIPV 120 >DQ667193-1|ABG75745.1| 510|Apis mellifera cys-loop ligand-gated ion channel subunit protein. Length = 510 Score = 22.2 bits (45), Expect = 4.7 Identities = 13/50 (26%), Positives = 22/50 (44%) Frame = -2 Query: 568 GYFLLDFLKSGLTVMVVQWNHFVYSYDELFDTEGESEQGMLTGLTVLRDT 419 GYFL+ + ++V+ W F + D G +LT T+ D+ Sbjct: 245 GYFLIQVYVPCVLIVVLSWVSFWIHREATSDRVGLGITTVLTLSTISLDS 294 >DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monooxygenase protein. Length = 517 Score = 21.8 bits (44), Expect = 6.2 Identities = 9/15 (60%), Positives = 11/15 (73%) Frame = -3 Query: 57 FSLPIFG*SRITNCV 13 FSLPIFG I +C+ Sbjct: 57 FSLPIFGTRWIFSCI 71 >AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic acetylcholine Apisa7-2 subunit protein. Length = 461 Score = 21.4 bits (43), Expect = 8.2 Identities = 8/24 (33%), Positives = 13/24 (54%) Frame = -2 Query: 664 FG*RLQHVVSVPSRNGHESDSSWV 593 FG L H++ V +N + + WV Sbjct: 35 FGLSLHHIIDVDEKNQILTTNCWV 58 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 195,631 Number of Sequences: 438 Number of extensions: 4111 Number of successful extensions: 25 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 19 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 21 length of database: 146,343 effective HSP length: 56 effective length of database: 121,815 effective search space used: 20586735 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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