Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0368
(677 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 262 2e-70
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 262 2e-70
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 262 2e-70
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 262 2e-70
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 140 6e-34
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 129 1e-30
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 77 1e-14
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 73 1e-13
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 42 5e-04
At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 42 5e-04
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 39 0.003
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 39 0.003
At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 38 0.005
At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 38 0.005
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 34 0.099
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 34 0.099
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 33 0.17
At4g11160.1 68417.m01808 translation initiation factor IF-2, mit... 33 0.23
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 33 0.23
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 33 0.23
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 32 0.40
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 31 0.93
At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 30 1.6
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 30 1.6
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 30 1.6
At5g28840.1 68418.m03547 NAD-dependent epimerase/dehydratase fam... 29 2.1
At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 29 2.1
At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s... 28 4.9
At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5) M... 28 6.5
At5g46380.1 68418.m05708 hypothetical protein 28 6.5
At3g19510.1 68416.m02472 homeobox protein (HAT 3.1) identical to... 28 6.5
At3g13020.1 68416.m01622 hAT dimerisation domain-containing prot... 28 6.5
At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 28 6.5
At3g05470.1 68416.m00599 formin homology 2 domain-containing pro... 27 8.6
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 262 bits (641), Expect = 2e-70
Identities = 130/211 (61%), Positives = 153/211 (72%)
Frame = +2
Query: 44 MGKEKTHINIVVIGHVDSGKSTTTGHLHLQMWWY*QTYHREVREGGPGNG*RILQICLGI 223
MGKEK HINIVVIGHVDSGKSTTTGHL ++ + + R + +
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 224 GQTKG*A*AWFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 403
+ K TIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 404 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMIPLNHHYSEPRFEEIKKEVSSYIKK 583
G FEAGISK+GQTREHALLAFTLGVKQ+I NKM YS+ R++EI KEVSSY+KK
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180
Query: 584 IGYNPAAVAFVPISGWHGDNMLEPSTKMPWF 676
+GYNP + FVPISG+ GDNM+E ST + W+
Sbjct: 181 VGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 262 bits (641), Expect = 2e-70
Identities = 130/211 (61%), Positives = 153/211 (72%)
Frame = +2
Query: 44 MGKEKTHINIVVIGHVDSGKSTTTGHLHLQMWWY*QTYHREVREGGPGNG*RILQICLGI 223
MGKEK HINIVVIGHVDSGKSTTTGHL ++ + + R + +
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 224 GQTKG*A*AWFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 403
+ K TIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 404 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMIPLNHHYSEPRFEEIKKEVSSYIKK 583
G FEAGISK+GQTREHALLAFTLGVKQ+I NKM YS+ R++EI KEVSSY+KK
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180
Query: 584 IGYNPAAVAFVPISGWHGDNMLEPSTKMPWF 676
+GYNP + FVPISG+ GDNM+E ST + W+
Sbjct: 181 VGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 262 bits (641), Expect = 2e-70
Identities = 130/211 (61%), Positives = 153/211 (72%)
Frame = +2
Query: 44 MGKEKTHINIVVIGHVDSGKSTTTGHLHLQMWWY*QTYHREVREGGPGNG*RILQICLGI 223
MGKEK HINIVVIGHVDSGKSTTTGHL ++ + + R + +
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 224 GQTKG*A*AWFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 403
+ K TIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 404 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMIPLNHHYSEPRFEEIKKEVSSYIKK 583
G FEAGISK+GQTREHALLAFTLGVKQ+I NKM YS+ R++EI KEVSSY+KK
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180
Query: 584 IGYNPAAVAFVPISGWHGDNMLEPSTKMPWF 676
+GYNP + FVPISG+ GDNM+E ST + W+
Sbjct: 181 VGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 262 bits (641), Expect = 2e-70
Identities = 130/211 (61%), Positives = 153/211 (72%)
Frame = +2
Query: 44 MGKEKTHINIVVIGHVDSGKSTTTGHLHLQMWWY*QTYHREVREGGPGNG*RILQICLGI 223
MGKEK HINIVVIGHVDSGKSTTTGHL ++ + + R + +
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 224 GQTKG*A*AWFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 403
+ K TIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 404 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMIPLNHHYSEPRFEEIKKEVSSYIKK 583
G FEAGISK+GQTREHALLAFTLGVKQ+I NKM YS+ R++EI KEVSSY+KK
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180
Query: 584 IGYNPAAVAFVPISGWHGDNMLEPSTKMPWF 676
+GYNP + FVPISG+ GDNM+E ST + W+
Sbjct: 181 VGYNPDKIPFVPISGFEGDNMIERSTNLDWY 211
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 140 bits (340), Expect = 6e-34
Identities = 77/199 (38%), Positives = 113/199 (56%), Gaps = 1/199 (0%)
Frame = +2
Query: 53 EKTHINIVVIGHVDSGKSTTTGHLHLQMWWY*QTYHREVREGGPGNG*RILQICLGIGQT 232
+K H+N+V IGHVD+GKST G + ++ + + +
Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157
Query: 233 KG*A*AWFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEF 412
+ T+++ FET TI+DAPGH+ ++ NMI+G SQAD VL+++A GEF
Sbjct: 158 EEERLKGKTVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKGEF 217
Query: 413 EAGISKNGQTREHALLAFTLGVKQLIVGVNKMIPLNHHYSEPRFEEIKKEVSSYIKKIGY 592
E G + GQTREH LA TLGV +LIV VNKM ++S+ R++EI++++ ++K GY
Sbjct: 218 ETGYERGGQTREHVQLAKTLGVSKLIVVVNKMDDPTVNWSKERYDEIEQKMVPFLKASGY 277
Query: 593 NPAA-VAFVPISGWHGDNM 646
N V F+PISG G NM
Sbjct: 278 NTKKDVVFLPISGLMGKNM 296
Score = 37.5 bits (83), Expect = 0.008
Identities = 15/43 (34%), Positives = 29/43 (67%)
Frame = +3
Query: 126 IYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERG 254
++ G +D R I+K+EKEA++ + S+ A+++D + ER +G
Sbjct: 122 LFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTNEEERLKG 164
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 129 bits (312), Expect = 1e-30
Identities = 69/200 (34%), Positives = 117/200 (58%), Gaps = 3/200 (1%)
Frame = +2
Query: 59 THINIVVIGHVDSGKSTTTGHL-HLQMWWY*QTYHREVREGG-PGNG*RILQICLGIGQT 232
+ +N+ ++GHVDSGKST +G L HL + H+ +E G G + ++
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKG--SFAYAWALDES 295
Query: 233 KG*A*AWFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEF 412
T+ +A+ F + +++V ++D+PGH+DF+ NMI G +QAD A+L++ A G F
Sbjct: 296 AEERERGITMTVAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAF 355
Query: 413 EAGISK-NGQTREHALLAFTLGVKQLIVGVNKMIPLNHHYSEPRFEEIKKEVSSYIKKIG 589
EAG GQTREHA + GV+Q+IV +NKM + YS+ RF+ IK+ V S+++
Sbjct: 356 EAGFDNLKGQTREHARVLRGFGVEQVIVAINKMDIVG--YSKERFDLIKQHVGSFLQSCR 413
Query: 590 YNPAAVAFVPISGWHGDNML 649
+ +++ ++P+S N++
Sbjct: 414 FKDSSLTWIPLSAMENQNLV 433
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 76.6 bits (180), Expect = 1e-14
Identities = 52/155 (33%), Positives = 77/155 (49%)
Frame = +2
Query: 41 KMGKEKTHINIVVIGHVDSGKSTTTGHLHLQMWWY*QTYHREVREGGPGNG*RILQICLG 220
K ++K H+NI IGHVD GK+T T L + + + ++ E R I
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDEIDAAPEERARGI--- 128
Query: 221 IGQTKG*A*AWFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 400
TI+ A ++ET + +D PGH D++KNMITG +Q D A+L+V+
Sbjct: 129 ------------TINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 176
Query: 401 TGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 505
G QT+EH LLA +GV ++V +NK
Sbjct: 177 DGPMP-------QTKEHILLAKQVGVPDMVVFLNK 204
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 73.3 bits (172), Expect = 1e-13
Identities = 58/198 (29%), Positives = 91/198 (45%)
Frame = +2
Query: 50 KEKTHINIVVIGHVDSGKSTTTGHLHLQMWWY*QTYHREVREGGPGNG*RILQICLGIGQ 229
+ K H+N+ IGHVD GK+T T + + + E G +I +
Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLTAAIT-----------KVLAEEGKAKAIAFDEIDKAPEE 111
Query: 230 TKG*A*AWFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGE 409
K TI A ++ET+K + +D PGH D++KNMITG +Q D +L+V+ G
Sbjct: 112 KK----RGITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGP 167
Query: 410 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMIPLNHHYSEPRFEEIKKEVSSYIKKIG 589
QT+EH LLA +GV L+ +NK+ ++ E +E+ S+ K G
Sbjct: 168 MP-------QTKEHILLARQVGVPSLVCFLNKVDVVDDPELLELVEMELRELLSFYKFPG 220
Query: 590 YNPAAVAFVPISGWHGDN 643
+ + +S G N
Sbjct: 221 DDIPIIRGSALSALQGTN 238
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 41.5 bits (93), Expect = 5e-04
Identities = 25/95 (26%), Positives = 47/95 (49%)
Frame = +2
Query: 287 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 466
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 467 TLGVKQLIVGVNKMIPLNHHYSEPRFEEIKKEVSS 571
+ +K +I+ NK+ +N + + E I+K +++
Sbjct: 178 MMRLKHIIILQNKIDLINEKAATEQHEAIQKFITN 212
>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 284
Score = 41.5 bits (93), Expect = 5e-04
Identities = 25/95 (26%), Positives = 47/95 (49%)
Frame = +2
Query: 287 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 466
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 467 TLGVKQLIVGVNKMIPLNHHYSEPRFEEIKKEVSS 571
+ +K +I+ NK+ +N + + E I+K +++
Sbjct: 178 MMRLKHIIILQNKIDLINEKAATEQHEAIQKFITN 212
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 39.1 bits (87), Expect = 0.003
Identities = 36/128 (28%), Positives = 55/128 (42%)
Frame = +2
Query: 68 NIVVIGHVDSGKSTTTGHLHLQMWWY*QTYHREVREGGPGNG*RILQICLGIGQTKG*A* 247
NI + H+DSGK+T T ++ +Y H E+ E +G + + + KG
Sbjct: 67 NIGISAHIDSGKTTLTE----RVLFYTGRIH-EIHEVRGRDGVGAKMDSMDLEREKG--- 118
Query: 248 AWFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 427
TI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 119 --ITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176
Query: 428 KNGQTREH 451
+ Q R +
Sbjct: 177 VDRQMRRY 184
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 39.1 bits (87), Expect = 0.003
Identities = 36/128 (28%), Positives = 55/128 (42%)
Frame = +2
Query: 68 NIVVIGHVDSGKSTTTGHLHLQMWWY*QTYHREVREGGPGNG*RILQICLGIGQTKG*A* 247
NI + H+DSGK+T T ++ +Y H E+ E +G + + + KG
Sbjct: 67 NIGISAHIDSGKTTLTE----RVLFYTGRIH-EIHEVRGRDGVGAKMDSMDLEREKG--- 118
Query: 248 AWFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 427
TI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 119 --ITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176
Query: 428 KNGQTREH 451
+ Q R +
Sbjct: 177 VDRQMRRY 184
>At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative
Length = 465
Score = 38.3 bits (85), Expect = 0.005
Identities = 23/91 (25%), Positives = 46/91 (50%)
Frame = +2
Query: 299 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGV 478
+V+ +D PGH + M+ G + D A+LI+AA QT EH + +
Sbjct: 120 HVSFVDCPGHDILMATMLNGAAIMDGALLIIAANE------TCPQPQTAEHLASVDMMHL 173
Query: 479 KQLIVGVNKMIPLNHHYSEPRFEEIKKEVSS 571
K +I+ NK+ + + + + E+I++ +++
Sbjct: 174 KDIIIIQNKIDLIQENEAIKQHEDIQRFITN 204
>At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to gb|U37354 from S. pombe. ESTs
gb|T41979, gb|N37284 and gb|N37529 come from this gene
Length = 465
Score = 38.3 bits (85), Expect = 0.005
Identities = 27/103 (26%), Positives = 49/103 (47%), Gaps = 4/103 (3%)
Frame = +2
Query: 281 FETSKY----YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTRE 448
FE SK +V+ +D PGH + M+ G + D A+L++AA QT E
Sbjct: 112 FENSKMKLLRHVSFVDCPGHDILMATMLNGAAIMDGALLLIAANE------TCPQPQTSE 165
Query: 449 HALLAFTLGVKQLIVGVNKMIPLNHHYSEPRFEEIKKEVSSYI 577
H + +K +I+ NK+ + + + + E I+K + + +
Sbjct: 166 HLAAVEIMQLKHIIILQNKIDLIQENVAINQHEAIQKFIMNTV 208
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 33.9 bits (74), Expect = 0.099
Identities = 18/49 (36%), Positives = 23/49 (46%)
Frame = +2
Query: 260 IDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 406
+ + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 197 MSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 33.9 bits (74), Expect = 0.099
Identities = 18/49 (36%), Positives = 23/49 (46%)
Frame = +2
Query: 260 IDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 406
+ + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 197 MSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 33.1 bits (72), Expect = 0.17
Identities = 16/41 (39%), Positives = 21/41 (51%)
Frame = +2
Query: 284 ETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 406
E S Y + +ID PGH DF + S A+L+V A G
Sbjct: 131 EASGYLLNLIDTPGHVDFSYEVSRSLSACQGALLVVDAAQG 171
>At4g11160.1 68417.m01808 translation initiation factor IF-2,
mitochondrial, putative similar to SP|P46198|IF2M_BOVIN
Translation initiation factor IF-2, mitochondrial
precursor (IF-2Mt) (IF-2(Mt)) {Bos taurus}
Length = 743
Score = 32.7 bits (71), Expect = 0.23
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +2
Query: 302 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 406
+T +D PGH F + G + D VL+VAA G
Sbjct: 270 ITFLDTPGHAAFSEMRARGAAVTDIVVLVVAADDG 304
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 32.7 bits (71), Expect = 0.23
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +2
Query: 296 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 406
Y + +ID+PGH DF + T +D A+++V A G
Sbjct: 74 YSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEG 110
Score = 27.5 bits (58), Expect = 8.6
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = +2
Query: 68 NIVVIGHVDSGKSTTTGHL 124
NI ++ HVD GK+T HL
Sbjct: 11 NICILAHVDHGKTTLADHL 29
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 32.7 bits (71), Expect = 0.23
Identities = 33/113 (29%), Positives = 51/113 (45%), Gaps = 5/113 (4%)
Frame = +2
Query: 68 NIVVIGHVDSGKSTTTGHLHLQMWWY*QTYHREVREGGPGNG*RILQICLGIG-QTKG*A 244
N+ VI HVD GKST T L Q +VR R + GI ++ G +
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDT----RADEAERGITIKSTGIS 76
Query: 245 *AWFTIDIALWKF----ETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 391
+ D +L F + ++Y + +ID+PGH DF + D A+++V
Sbjct: 77 LYYEMTDESLKSFTGARDGNEYLINLIDSPGHVDFSSEVTAALRITDGALVVV 129
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 31.9 bits (69), Expect = 0.40
Identities = 32/118 (27%), Positives = 51/118 (43%), Gaps = 1/118 (0%)
Frame = +2
Query: 56 KTHINIVVIGHVDSGKSTTTGHLHLQMWWY*QTYH-REVREGGPGNG*RILQICLGIGQT 232
K + NI ++ H+D+GK+TTT + +++ + Y EV EG + Q
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERI---LYYTGRNYKIGEVHEG------TATMDWMEQEQE 144
Query: 233 KG*A*AWFTIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 406
+G TI A K+ + IID PGH DF + D A+ + + G
Sbjct: 145 RG-----ITITSAATTTFWDKHRINIIDTPGHVDFTLEVERALRVLDGAICLFDSVAG 197
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 30.7 bits (66), Expect = 0.93
Identities = 13/42 (30%), Positives = 23/42 (54%)
Frame = +2
Query: 281 FETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 406
+E + + + +ID PGH DF + + + A+L+V A G
Sbjct: 147 YEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQG 188
>At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profile PF00009: Elongation
factor Tu GTP binding domain
Length = 630
Score = 29.9 bits (64), Expect = 1.6
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 308 IIDAPGHRDFIKNMITGTSQADCAVLIV 391
+ID PGH F G+S D A+L+V
Sbjct: 113 VIDTPGHESFTNLRSRGSSLCDLAILVV 140
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 29.9 bits (64), Expect = 1.6
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 308 IIDAPGHRDFIKNMITGTSQADCAVLIV 391
+ID PGH F G+S D A+L+V
Sbjct: 772 VIDTPGHESFTNLRSRGSSLCDLAILVV 799
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 29.9 bits (64), Expect = 1.6
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 308 IIDAPGHRDFIKNMITGTSQADCAVLIV 391
+ID PGH F G+S D A+L+V
Sbjct: 705 VIDTPGHESFTNLRSRGSSLCDLAILVV 732
>At5g28840.1 68418.m03547 NAD-dependent epimerase/dehydratase family
protein similar to sugar epimerase BlmG from
Streptomyces verticillus GI:9937230; contains Pfam
profile PF01370 NAD dependent epimerase/dehydratase
family
Length = 377
Score = 29.5 bits (63), Expect = 2.1
Identities = 12/33 (36%), Positives = 21/33 (63%)
Frame = +3
Query: 210 YAWVLDKLKAERERGSQSILLSGSSKLASTMLP 308
Y W+ ++++ E+ +GS + L GSSK+ T P
Sbjct: 333 YFWIKEQIEKEKAKGS-DVSLYGSSKVVGTQAP 364
>At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1088
Score = 29.5 bits (63), Expect = 2.1
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = +2
Query: 302 VTIIDAPGHRDFIKNMITGTSQADCAVLIV 391
+ +ID PGH F G++ D A+L+V
Sbjct: 558 ILVIDTPGHESFTNLRSRGSNLCDLAILVV 587
>At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein
similar to 1,4-alpha-glucan branching enzyme [Solanum
tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme
(EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum
tuberosum} SP|P30924; contains Pfam profiles: PF00128
Alpha amylase catalytic domain, PF02922 Isoamylase
N-terminal domain
Length = 777
Score = 28.3 bits (60), Expect = 4.9
Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 3/33 (9%)
Frame = +2
Query: 530 SEPR---FEEIKKEVSSYIKKIGYNPAAVAFVP 619
SEP+ FEE K+V ++K+ GYN + VP
Sbjct: 257 SEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVP 289
>At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5)
Monovalent cation:proton antiporter family 2 (CPA2
family) member, PMID:11500563; related to
glutathione-regulated potassium-efflux system protein
[Escherichia coli] GP|606284|gb|AAA58147
Length = 568
Score = 27.9 bits (59), Expect = 6.5
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +2
Query: 536 PRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNM 646
PRF ++ ++SS ++ Y AAVAF +S W D +
Sbjct: 354 PRFLKLMIQLSSQTNEL-YQLAAVAFCLLSAWCSDKL 389
>At5g46380.1 68418.m05708 hypothetical protein
Length = 607
Score = 27.9 bits (59), Expect = 6.5
Identities = 20/49 (40%), Positives = 27/49 (55%), Gaps = 6/49 (12%)
Frame = -3
Query: 285 SNFQRAISIVNHA-HAQPLVCPIPKHI*RILYPFP-----GPPSRTSRW 157
S FQ+ + N A H +PLV P+P H R YP P GP +R+S +
Sbjct: 472 SAFQQHGGLNNDAYHLRPLVAPLPLH--RDSYPCPPTLAAGPYTRSSAY 518
>At3g19510.1 68416.m02472 homeobox protein (HAT 3.1) identical to
homeotic protein HAT 3.1 (GI:11994474) [Arabidopsis
thaliana]
Length = 723
Score = 27.9 bits (59), Expect = 6.5
Identities = 10/49 (20%), Positives = 23/49 (46%)
Frame = +2
Query: 500 NKMIPLNHHYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNM 646
NK + + + IKK++ ++ +I Y + + + GW G ++
Sbjct: 163 NKTMNKGQVREDDEYTRIKKKLRYFLNRINYEQSLIDAYSLEGWKGSSL 211
>At3g13020.1 68416.m01622 hAT dimerisation domain-containing protein
contains Pfam profile: PF04937 domain of unknown
function (DUF659), weak hit to PF05699: hAT family
dimerisation domain
Length = 605
Score = 27.9 bits (59), Expect = 6.5
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = +2
Query: 485 LIVGVNKMIPLNHHYSEPRFEEIKKEVSSYIKKI 586
+ +GV + IP +H + +E KEV Y+K I
Sbjct: 152 MALGVGQKIPDSHDLNGRLLQEAMKEVQDYVKNI 185
>At1g17220.1 68414.m02098 translation initiation factor IF-2,
chloroplast, putative similar to SP|P57997|IF2C_PHAVU
Translation initiation factor IF-2, chloroplast
precursor (PvIF2cp) {Phaseolus vulgaris}
Length = 1026
Score = 27.9 bits (59), Expect = 6.5
Identities = 12/32 (37%), Positives = 16/32 (50%)
Frame = +2
Query: 311 IDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 406
+D PGH F G D A+++VAA G
Sbjct: 557 LDTPGHEAFGAMRARGARVTDIAIIVVAADDG 588
>At3g05470.1 68416.m00599 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 884
Score = 27.5 bits (58), Expect = 8.6
Identities = 26/94 (27%), Positives = 38/94 (40%)
Frame = -2
Query: 394 SYDEHSAISLRGSCDHVLDEISVSRSINDGNIVLASFELPESNIDCEPRSRSAFSLSNTQ 215
S D+ S S+ G + +S + S + V +S E +D SRS F +S +
Sbjct: 308 SDDDESFHSVGGGSQYSNPRLSNASSASGSVNVGSSQRFSEHKLDIPECSRSDFGISVSA 367
Query: 214 AYLKDPLPISWASFSNFSMVRLSIPPHL*MQVTS 113
P P FSN + LS P +Q S
Sbjct: 368 PPPPPPPPPPLPQFSNKRIHTLSSPETANLQTLS 401
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,156,565
Number of Sequences: 28952
Number of extensions: 317066
Number of successful extensions: 961
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 898
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 940
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1428369392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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