BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0344
(624 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC607.08c |||DUF726 family protein|Schizosaccharomyces pombe|c... 27 2.9
SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S... 26 3.8
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 26 5.1
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 26 5.1
>SPAC607.08c |||DUF726 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 579
Score = 26.6 bits (56), Expect = 2.9
Identities = 16/45 (35%), Positives = 22/45 (48%), Gaps = 1/45 (2%)
Frame = -2
Query: 368 AGSATI-TGATLGPSATITGLGTMMAGSAMITGETAGFSQHLIVQ 237
AG T+ G LG T LGT++ + MIT GF + +Q
Sbjct: 199 AGLGTLFAGLGLGTMIGATYLGTLITSAPMITALFGGFGAKMSMQ 243
>SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 971
Score = 26.2 bits (55), Expect = 3.8
Identities = 13/35 (37%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
Frame = +2
Query: 95 GPALVEA-PIVPSPVHVGPLVPGQLTPLVHILINI 196
G AL+ PIV S H+ P G+L P +H +++
Sbjct: 781 GAALLSKFPIVNSTHHLLPSPQGELAPAIHATLDV 815
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 25.8 bits (54), Expect = 5.1
Identities = 21/70 (30%), Positives = 22/70 (31%), Gaps = 4/70 (5%)
Frame = -1
Query: 522 RGHNGGSAXXXXXXXXXXXRALG*----GHNGRVSHDHGGHQGHVTNVHWARGHNGGVSH 355
RGHNGG G G G H HGGH G G GG
Sbjct: 183 RGHNGGGFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGG 242
Query: 354 DHRGYTRSLG 325
G+ LG
Sbjct: 243 GPGGFGGGLG 252
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 25.8 bits (54), Expect = 5.1
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +2
Query: 86 PDVGPALVEAPIVPSPVHVGPLVP 157
P V P + EAP VP P V P+ P
Sbjct: 592 PPVAPVVPEAPSVPQP-PVAPVAP 614
Score = 25.0 bits (52), Expect = 8.9
Identities = 12/24 (50%), Positives = 13/24 (54%)
Frame = +2
Query: 86 PDVGPALVEAPIVPSPVHVGPLVP 157
P V P E P VP P V P+VP
Sbjct: 577 PPVAPVAPEVPSVPQP-PVAPVVP 599
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,796,032
Number of Sequences: 5004
Number of extensions: 30395
Number of successful extensions: 99
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 91
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 98
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 275671126
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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