BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0340 (718 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 30 0.38 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 28 1.5 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 27 2.7 SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor ... 27 2.7 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 27 3.5 SPBC2A9.03 |||conserved protein |Schizosaccharomyces pombe|chr 2... 26 4.7 SPAC343.19 ||SPAC824.01|phosphatidylinositol 4-kinase Lsb6 |Schi... 26 4.7 SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 26 6.2 SPBC354.10 |||RNAPII degradation factor |Schizosaccharomyces pom... 25 8.2 SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence orphan|Schizos... 25 8.2 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 25 8.2 SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual 25 8.2 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 29.9 bits (64), Expect = 0.38 Identities = 22/84 (26%), Positives = 34/84 (40%), Gaps = 1/84 (1%) Frame = -1 Query: 610 TSNPVGSATSLSSSVTDNVRAQAEANQVDSTPIAPPLLMSQTKKLPRNCPTLGTPLTAV- 434 TS PV S +++ T + + V STP+ + T + P TPLT Sbjct: 436 TSTPVTSTPLATTNCTTSTSVPYTSTPVTSTPLTTTNCTTSTSIPYTSTPVTSTPLTTTN 495 Query: 433 LYRPLARPRQSTTITLQSETASKA 362 + P ST +T + T S + Sbjct: 496 CTTSTSVPYTSTPVTSSNYTISSS 519 Score = 27.9 bits (59), Expect = 1.5 Identities = 20/57 (35%), Positives = 27/57 (47%) Frame = -1 Query: 610 TSNPVGSATSLSSSVTDNVRAQAEANQVDSTPIAPPLLMSQTKKLPRNCPTLGTPLT 440 TS P G ++SLS+ +T V + ++ S PI PP S T PT T T Sbjct: 233 TSIPTGGSSSLSTPITPTVPPTSTSS--TSIPI-PPTSTSSTDTNSSPLPTTSTSCT 286 Score = 27.1 bits (57), Expect = 2.7 Identities = 20/73 (27%), Positives = 31/73 (42%), Gaps = 1/73 (1%) Frame = -1 Query: 577 SSSVTDNVRAQAEANQVDSTPIAPPLLMSQTKKLPRNCPTLGTPL-TAVLYRPLARPRQS 401 S+ VT + + + V STP+ + T L + P TPL T + P S Sbjct: 506 STPVTSSNYTISSSTPVTSTPVTTTNCTTSTSVLYTSTPVTSTPLATTNCTTSTSVPYTS 565 Query: 400 TTITLQSETASKA 362 T +T + T S + Sbjct: 566 TPVTSSNYTISSS 578 Score = 26.2 bits (55), Expect = 4.7 Identities = 21/76 (27%), Positives = 32/76 (42%), Gaps = 2/76 (2%) Frame = -1 Query: 610 TSNPVGSATSLSSS-VTDNVRAQAEANQVDSTPIAPPLLMSQTKKLPRNCPTLGTPLTAV 434 T P S+T L+++ T + + V STP+A + T + P TPLT Sbjct: 412 TVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTSVPYTSTPVTSTPLTTT 471 Query: 433 -LYRPLARPRQSTTIT 389 + P ST +T Sbjct: 472 NCTTSTSIPYTSTPVT 487 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 27.9 bits (59), Expect = 1.5 Identities = 13/44 (29%), Positives = 20/44 (45%) Frame = -2 Query: 639 TTPPGPAGSKPVTLLGLPPACRPALPTTCAPKLKPIKWTRPQLP 508 + PP P+ + P+ LPP +PA P P + P +P Sbjct: 156 SAPPIPSKAPPIPS-SLPPPAQPAAPVKSPPSAPSLPSAVPPMP 198 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 27.1 bits (57), Expect = 2.7 Identities = 12/28 (42%), Positives = 17/28 (60%) Frame = -2 Query: 591 LPPACRPALPTTCAPKLKPIKWTRPQLP 508 L P+ P LP + +L P+ TRPQ+P Sbjct: 1046 LSPSVTPQLPPVSS-RLPPVSATRPQIP 1072 >SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor Ste6|Schizosaccharomyces pombe|chr 3|||Manual Length = 911 Score = 27.1 bits (57), Expect = 2.7 Identities = 10/32 (31%), Positives = 20/32 (62%) Frame = +2 Query: 170 LCQGTVQTTNTGFSPDVTKTTIKLLQMASQLY 265 LC +VQ+ +T PD+T + + +L ++ + Y Sbjct: 248 LCSNSVQSAHTNLQPDIT-SFVAILSLSHEAY 278 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 26.6 bits (56), Expect = 3.5 Identities = 13/37 (35%), Positives = 22/37 (59%) Frame = +1 Query: 70 SHYVEGVSRYVWMPDGEGVPHLVDLEETAEEDILMSR 180 S Y +G+S V + G+GV H+V + E+ + L+ R Sbjct: 141 SLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVGR 177 >SPBC2A9.03 |||conserved protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 426 Score = 26.2 bits (55), Expect = 4.7 Identities = 10/19 (52%), Positives = 11/19 (57%) Frame = -1 Query: 106 SKRIGLHLPHSDCCLQGSW 50 S + LPHSD L GSW Sbjct: 290 SNSVSAKLPHSDSVLTGSW 308 >SPAC343.19 ||SPAC824.01|phosphatidylinositol 4-kinase Lsb6 |Schizosaccharomyces pombe|chr 1|||Manual Length = 624 Score = 26.2 bits (55), Expect = 4.7 Identities = 13/42 (30%), Positives = 25/42 (59%) Frame = +3 Query: 237 SYYKWQVNSIRNSNYNGNLPLFVIVHGWNSNGNSAVNTMIRP 362 S+ +Q NS+ N+N PL + GW S+ +S+ ++++ P Sbjct: 12 SFPNYQENSL-NTNEEQTNPLESLRDGWASSNSSSSSSLLLP 52 >SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 670 Score = 25.8 bits (54), Expect = 6.2 Identities = 16/38 (42%), Positives = 19/38 (50%) Frame = +1 Query: 412 VLPTVYTTLPSMEFPVSDSSLATFWSGSSTTEGQLGSS 525 VLPT T S VS SSL++F ST GS+ Sbjct: 244 VLPTSIITSTSTPVTVSSSSLSSFTPSYSTNLTTTGST 281 >SPBC354.10 |||RNAPII degradation factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 963 Score = 25.4 bits (53), Expect = 8.2 Identities = 10/29 (34%), Positives = 18/29 (62%) Frame = -1 Query: 622 SRIQTSNPVGSATSLSSSVTDNVRAQAEA 536 S QTSNP+ +AT+ +S +T+ ++ Sbjct: 810 SGTQTSNPINNATANTSGMTEKAAGHRDS 838 >SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 1563 Score = 25.4 bits (53), Expect = 8.2 Identities = 15/35 (42%), Positives = 15/35 (42%) Frame = -2 Query: 690 CGLPPHKLCVTVQGLVVTTPPGPAGSKPVTLLGLP 586 C P L VTV TT G GS TL G P Sbjct: 188 CPPPTTILIVTVPTTTTTTTVGYPGSVTTTLTGTP 222 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 25.4 bits (53), Expect = 8.2 Identities = 14/34 (41%), Positives = 19/34 (55%) Frame = -2 Query: 633 PPGPAGSKPVTLLGLPPACRPALPTTCAPKLKPI 532 PP + S+ V+ PPA PA+P AP L P+ Sbjct: 376 PPPLSSSRAVSN---PPAPPPAIPGRSAPALPPL 406 >SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual Length = 978 Score = 25.4 bits (53), Expect = 8.2 Identities = 13/26 (50%), Positives = 17/26 (65%) Frame = +3 Query: 258 NSIRNSNYNGNLPLFVIVHGWNSNGN 335 +SIRNS++N L LF V S+GN Sbjct: 604 SSIRNSDFNSQLYLFETVGVLISSGN 629 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,337,995 Number of Sequences: 5004 Number of extensions: 72198 Number of successful extensions: 269 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 245 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 267 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 335201398 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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