Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0337
(560 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 149 2e-37
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 149 2e-37
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 149 2e-37
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 46 5e-06
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 40 3e-04
SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr 1|||Ma... 28 0.82
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 1.9
SPBC18H10.20c |||conserved fungal protein|Schizosaccharomyces po... 25 5.8
SPAC57A7.11 |mip1||WD repeat protein Mip1|Schizosaccharomyces po... 25 7.6
SPAC7D4.04 |taf1||Taz1 interacting factor 1|Schizosaccharomyces ... 25 7.6
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 25 7.6
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 149 bits (362), Expect = 2e-37
Identities = 83/183 (45%), Positives = 98/183 (53%), Gaps = 1/183 (0%)
Frame = +3
Query: 3 NPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARPTD 182
NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G GK L+EA+D+I PPARPTD
Sbjct: 182 NPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTD 241
Query: 183 KPLRLPLQDVYKIGGIGTVPVGRVKL-VC*NQVXXXXXXXXXXXXXXXXWRAPRSSPRSC 359
KPLRLPLQDVYKIGGIGTVPVGRV+ V + S
Sbjct: 242 KPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGL 301
Query: 360 TWRQCRFQRKERVRQGIASWLCCW*LQKQPT*GCCRFYSSSHCA*PSWSNSNGYTPVLDC 539
F K + I C + P GC F + S GY+PVLDC
Sbjct: 302 PGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDC 361
Query: 540 HTA 548
HTA
Sbjct: 362 HTA 364
Score = 102 bits (245), Expect = 3e-23
Identities = 44/58 (75%), Positives = 50/58 (86%)
Frame = +1
Query: 334 HHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQ 507
HHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQ
Sbjct: 293 HHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQ 350
Score = 45.6 bits (103), Expect = 5e-06
Identities = 20/25 (80%), Positives = 22/25 (88%)
Frame = +2
Query: 257 TGVLKPGTIVVFAPANITTEVKSVE 331
TGV+KPG IV FAPA +TTEVKSVE
Sbjct: 267 TGVIKPGMIVTFAPAGVTTEVKSVE 291
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 149 bits (362), Expect = 2e-37
Identities = 83/183 (45%), Positives = 98/183 (53%), Gaps = 1/183 (0%)
Frame = +3
Query: 3 NPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARPTD 182
NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G GK L+EA+D+I PPARPTD
Sbjct: 182 NPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTD 241
Query: 183 KPLRLPLQDVYKIGGIGTVPVGRVKL-VC*NQVXXXXXXXXXXXXXXXXWRAPRSSPRSC 359
KPLRLPLQDVYKIGGIGTVPVGRV+ V + S
Sbjct: 242 KPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGL 301
Query: 360 TWRQCRFQRKERVRQGIASWLCCW*LQKQPT*GCCRFYSSSHCA*PSWSNSNGYTPVLDC 539
F K + I C + P GC F + S GY+PVLDC
Sbjct: 302 PGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDC 361
Query: 540 HTA 548
HTA
Sbjct: 362 HTA 364
Score = 102 bits (245), Expect = 3e-23
Identities = 44/58 (75%), Positives = 50/58 (86%)
Frame = +1
Query: 334 HHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQ 507
HHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQ
Sbjct: 293 HHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQ 350
Score = 45.6 bits (103), Expect = 5e-06
Identities = 20/25 (80%), Positives = 22/25 (88%)
Frame = +2
Query: 257 TGVLKPGTIVVFAPANITTEVKSVE 331
TGV+KPG IV FAPA +TTEVKSVE
Sbjct: 267 TGVIKPGMIVTFAPAGVTTEVKSVE 291
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 149 bits (362), Expect = 2e-37
Identities = 83/183 (45%), Positives = 98/183 (53%), Gaps = 1/183 (0%)
Frame = +3
Query: 3 NPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARPTD 182
NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G GK L+EA+D+I PPARPTD
Sbjct: 182 NPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTD 241
Query: 183 KPLRLPLQDVYKIGGIGTVPVGRVKL-VC*NQVXXXXXXXXXXXXXXXXWRAPRSSPRSC 359
KPLRLPLQDVYKIGGIGTVPVGRV+ V + S
Sbjct: 242 KPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGL 301
Query: 360 TWRQCRFQRKERVRQGIASWLCCW*LQKQPT*GCCRFYSSSHCA*PSWSNSNGYTPVLDC 539
F K + I C + P GC F + S GY+PVLDC
Sbjct: 302 PGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDC 361
Query: 540 HTA 548
HTA
Sbjct: 362 HTA 364
Score = 102 bits (245), Expect = 3e-23
Identities = 44/58 (75%), Positives = 50/58 (86%)
Frame = +1
Query: 334 HHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQ 507
HHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQ
Sbjct: 293 HHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQ 350
Score = 45.6 bits (103), Expect = 5e-06
Identities = 20/25 (80%), Positives = 22/25 (88%)
Frame = +2
Query: 257 TGVLKPGTIVVFAPANITTEVKSVE 331
TGV+KPG IV FAPA +TTEVKSVE
Sbjct: 267 TGVIKPGMIVTFAPAGVTTEVKSVE 291
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 45.6 bits (103), Expect = 5e-06
Identities = 31/83 (37%), Positives = 43/83 (51%), Gaps = 2/83 (2%)
Frame = +3
Query: 15 VAFVPISGWHGDNMLEPSTK--MPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKP 188
V FVPIS G N+++ + W+KG L+ ALD ++PP +P KP
Sbjct: 357 VHFVPISAISGTNLIQKDSSDLYKWYKG------------PTLLSALDQLVPPEKPYRKP 404
Query: 189 LRLPLQDVYKIGGIGTVPVGRVK 257
LRL + DVY+ TV GRV+
Sbjct: 405 LRLSIDDVYRSPRSVTV-TGRVE 426
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 39.9 bits (89), Expect = 3e-04
Identities = 19/42 (45%), Positives = 28/42 (66%), Gaps = 1/42 (2%)
Frame = +3
Query: 135 LIEALDAILP-PARPTDKPLRLPLQDVYKIGGIGTVPVGRVK 257
L+EA+D+ + P R TD P + ++DV+ I G GTV GRV+
Sbjct: 234 LMEAVDSYITLPERKTDVPFLMAIEDVFSISGRGTVVTGRVE 275
Score = 26.6 bits (56), Expect = 2.5
Identities = 10/27 (37%), Positives = 18/27 (66%)
Frame = +1
Query: 346 LQEAVPGDNVGFNVKNVSVKELRRGYV 426
L AV GDN G ++++ ++L+RG +
Sbjct: 308 LDAAVAGDNCGLLLRSIKREQLKRGMI 334
>SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 835
Score = 28.3 bits (60), Expect = 0.82
Identities = 15/46 (32%), Positives = 19/46 (41%)
Frame = -2
Query: 175 GRAGGRMASRASMRHFPSALPSLRSTCHPLNQGILVEGSNMLSPCH 38
G GG S+ P+ LPS + P L E +ML CH
Sbjct: 545 GAGGGHAPDIISLVQNPNILPSSTNPTRPFTTNTLDEELDMLMVCH 590
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 27.1 bits (57), Expect = 1.9
Identities = 13/28 (46%), Positives = 16/28 (57%)
Frame = -3
Query: 411 QFLDGHVLYVETYIVSRYSFLESFVVLS 328
+F DGH+L ETY+ LES V S
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGVFRS 554
>SPBC18H10.20c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 361
Score = 25.4 bits (53), Expect = 5.8
Identities = 13/45 (28%), Positives = 19/45 (42%)
Frame = -1
Query: 374 TLSPGTASWRASWCSPQTLLXXXXXXXXXXXWYLVSTHQFNSADG 240
T PGT W SW P +L +YL +T + + +G
Sbjct: 101 TYRPGTQHWPFSWLFPGSLPASVSNRYIKLEYYLEATLCYGTPEG 145
>SPAC57A7.11 |mip1||WD repeat protein Mip1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1313
Score = 25.0 bits (52), Expect = 7.6
Identities = 20/62 (32%), Positives = 27/62 (43%)
Frame = +1
Query: 4 TQLLSLSCPFLDGTETTCWSLQPKCLGSRDGRWSVRKAKLTENASLKLSMPSCHLPAPLT 183
T L L+ F T+T W++ PK L R R + A L N L + H P +
Sbjct: 360 TPLGELNWIFTAITDTIAWNVFPKHLFRRLFRQDLMVAALFRNFLLAERIMLVHSCHPQS 419
Query: 184 SP 189
SP
Sbjct: 420 SP 421
>SPAC7D4.04 |taf1||Taz1 interacting factor 1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 926
Score = 25.0 bits (52), Expect = 7.6
Identities = 9/16 (56%), Positives = 12/16 (75%)
Frame = -2
Query: 265 HTSLTLPTGTVPIPPI 218
H L LPTG++PI P+
Sbjct: 182 HDFLALPTGSLPITPL 197
>SPBC1539.08 |||ADP-ribosylation factor, Arf
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 25.0 bits (52), Expect = 7.6
Identities = 14/37 (37%), Positives = 20/37 (54%)
Frame = +1
Query: 34 LDGTETTCWSLQPKCLGSRDGRWSVRKAKLTENASLK 144
LD + W++QP C + DG A L++NA LK
Sbjct: 149 LDKLKDRLWNVQPTCALTGDGLLE-GLAWLSQNAKLK 184
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,623,475
Number of Sequences: 5004
Number of extensions: 55602
Number of successful extensions: 179
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 179
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 236012634
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -