Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0324
(662 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 164 1e-41
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 105 8e-24
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 101 9e-23
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 90 3e-19
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 61 1e-10
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 58 1e-09
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 54 1e-08
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 47 2e-06
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 38 0.001
SPBC17D11.06 |spp2|pri2|DNA primase large subunit Spp2 |Schizosa... 29 0.79
SPAC144.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 27 1.8
SPCC306.05c |ins1||INSIG domain protein|Schizosaccharomyces pomb... 27 1.8
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 3.2
SPBC18E5.09c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 26 4.2
SPBC2G5.06c |hmt2|cad1|sulfide-quinone oxidoreductase|Schizosacc... 25 7.4
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 164 bits (398), Expect = 1e-41
Identities = 76/84 (90%), Positives = 81/84 (96%)
Frame = +2
Query: 2 PEEHPVLLTEAPLNPKANREKMTQIMFXTFNSPAMYVAIQAVLSLYASGRTTGIVLDSGD 181
PEEHP LLTEAPLNPK+NREKMTQI+F TFN+PA YVAIQAVLSLYASGRTTGIVLDSGD
Sbjct: 98 PEEHPCLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGD 157
Query: 182 GVSHTVPIYEGYALPHAILRLDLA 253
GV+HTVPIYEGYALPHAI+RLDLA
Sbjct: 158 GVTHTVPIYEGYALPHAIMRLDLA 181
Score = 153 bits (372), Expect = 2e-38
Identities = 74/86 (86%), Positives = 78/86 (90%)
Frame = +1
Query: 247 LGCRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDLXQEMATAAASTSLEKS 426
L RDLTDYLMKIL ERGY+F+TTAEREIVRDIKEKLCYVALD QE+ TAA S+SLEKS
Sbjct: 180 LAGRDLTDYLMKILMERGYTFSTTAEREIVRDIKEKLCYVALDFEQELQTAAQSSSLEKS 239
Query: 427 YELPDGQVITIGNERFRCPEALFQPS 504
YELPDGQVITIGNERFR PEALFQPS
Sbjct: 240 YELPDGQVITIGNERFRAPEALFQPS 265
Score = 54.4 bits (125), Expect = 1e-08
Identities = 27/48 (56%), Positives = 31/48 (64%), Gaps = 1/48 (2%)
Frame = +3
Query: 510 GMESCGIHETVYNSIMKCDVDIRKTC-TPTPSCPVVHMYPGIADRCRR 650
G+E+ GIHE YNSIMKCDVDIRK MYPGIADR ++
Sbjct: 268 GLENAGIHEATYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIADRMQK 315
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 105 bits (251), Expect = 8e-24
Identities = 48/84 (57%), Positives = 61/84 (72%)
Frame = +2
Query: 2 PEEHPVLLTEAPLNPKANREKMTQIMFXTFNSPAMYVAIQAVLSLYASGRTTGIVLDSGD 181
P +LLTE P+NP ANREKM + MF + +YVAIQAVLSLYA G ++G+V+DSGD
Sbjct: 98 PRGRKILLTEPPMNPVANREKMCETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGD 157
Query: 182 GVSHTVPIYEGYALPHAILRLDLA 253
GV+H VP+YE L H + RLD+A
Sbjct: 158 GVTHIVPVYESVVLNHLVGRLDVA 181
Score = 96.7 bits (230), Expect = 3e-21
Identities = 42/82 (51%), Positives = 59/82 (71%)
Frame = +1
Query: 256 RDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDLXQEMATAAASTSLEKSYEL 435
RD T YL+ +L +GY+F TA+ E VR++KEKLCYV+ DL + + +T L ++Y L
Sbjct: 183 RDATRYLISLLLRKGYAFNRTADFETVREMKEKLCYVSYDLELDHKLSEETTVLMRNYTL 242
Query: 436 PDGQVITIGNERFRCPEALFQP 501
PDG+VI +G+ER+ CPE LFQP
Sbjct: 243 PDGRVIKVGSERYECPECLFQP 264
Score = 28.3 bits (60), Expect = 1.0
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +3
Query: 504 LPGMESCGIHETVYNSIMKCDVDIRK 581
L G E G+ E ++++I DVDIRK
Sbjct: 266 LVGSEQPGLSEFIFDTIQAADVDIRK 291
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 101 bits (242), Expect = 9e-23
Identities = 44/78 (56%), Positives = 61/78 (78%)
Frame = +2
Query: 2 PEEHPVLLTEAPLNPKANREKMTQIMFXTFNSPAMYVAIQAVLSLYASGRTTGIVLDSGD 181
PEEHP+LLTE PL N+EK+ + + T N PA+ ++Q VL+LYAS RTTGIVL+ GD
Sbjct: 103 PEEHPLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGD 162
Query: 182 GVSHTVPIYEGYALPHAI 235
G++H+VPIY+G+++P AI
Sbjct: 163 GLTHSVPIYDGFSIPSAI 180
Score = 85.0 bits (201), Expect = 9e-18
Identities = 40/85 (47%), Positives = 53/85 (62%)
Frame = +1
Query: 247 LGCRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDLXQEMATAAASTSLEKS 426
+G RD+TDYL L + G+ ++AE+EIVR+IKEK CYVA D E+ + +
Sbjct: 185 IGGRDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYVASDFRSEIESWTEHKPQIHT 244
Query: 427 YELPDGQVITIGNERFRCPEALFQP 501
Y+LPD Q IT+G E F PE LF P
Sbjct: 245 YQLPDNQTITLGTECFSAPEVLFNP 269
Score = 28.3 bits (60), Expect = 1.0
Identities = 10/25 (40%), Positives = 17/25 (68%)
Frame = +3
Query: 510 GMESCGIHETVYNSIMKCDVDIRKT 584
G E+ G+H ++ SI+ D+D+R T
Sbjct: 273 GSEASGLHIQLFKSILLSDIDLRST 297
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 89.8 bits (213), Expect = 3e-19
Identities = 45/92 (48%), Positives = 60/92 (65%), Gaps = 8/92 (8%)
Frame = +2
Query: 2 PEEHPVLLTEAPLNPKANREKMTQIMFXTFNSPAMYVAIQAVLSLYASGRT--------T 157
PE+H LLTE PLNP NRE +IMF +FN +Y+A+QAVL+L AS + T
Sbjct: 121 PEDHYFLLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLT 180
Query: 158 GIVLDSGDGVSHTVPIYEGYALPHAILRLDLA 253
G V+DSGDGV+H +P+ EGY + +I + LA
Sbjct: 181 GTVVDSGDGVTHIIPVAEGYVIGSSIKTMPLA 212
Score = 37.1 bits (82), Expect = 0.002
Identities = 32/91 (35%), Positives = 43/91 (47%), Gaps = 6/91 (6%)
Frame = +1
Query: 247 LGCRDLTDYLMKILTERGY--SFTTTAEREIVRDIKEKLCYVALDLXQEMATAAASTS-- 414
L RD+T ++ +L +R S TAER IKE+ CYV D+ +E +
Sbjct: 211 LAGRDVTYFVQSLLRDRNEPDSSLKTAER-----IKEECCYVCPDIVKEFSRFDREPDRY 265
Query: 415 LEKSYELPDGQVITI--GNERFRCPEALFQP 501
L+ + E G TI G ERF PE F P
Sbjct: 266 LKYASESITGHSTTIDVGFERFLAPEIFFNP 296
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 61.3 bits (142), Expect = 1e-10
Identities = 30/74 (40%), Positives = 42/74 (56%)
Frame = +2
Query: 2 PEEHPVLLTEAPLNPKANREKMTQIMFXTFNSPAMYVAIQAVLSLYASGRTTGIVLDSGD 181
P EHP+L+TE NP NR K + MF + PA Y+A Q + +ASG+ T ++D G
Sbjct: 96 PLEHPILITEPFDNPPENRVKTLETMFESLRCPATYLAKQETCAAFASGKGTACLVDIGA 155
Query: 182 GVSHTVPIYEGYAL 223
S IY+G+ L
Sbjct: 156 ERSSVSAIYDGFVL 169
Score = 35.5 bits (78), Expect = 0.007
Identities = 15/42 (35%), Positives = 24/42 (57%)
Frame = +1
Query: 376 LXQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQP 501
L ++ ++ +T E +E PDG + G ER++ PE LF P
Sbjct: 247 LISDVPFSSETTIAESEFEFPDGSRMMFGAERYQIPEHLFVP 288
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 58.0 bits (134), Expect = 1e-09
Identities = 25/71 (35%), Positives = 43/71 (60%), Gaps = 2/71 (2%)
Frame = +2
Query: 8 EHPVLLTEAPLNPKANREKMTQIMFXTFNSPAMYVAIQAVLSLYASGR--TTGIVLDSGD 181
EHP+ +TE NP R MT+++F +N+P++ I + S Y + + ++GIVL+ G+
Sbjct: 115 EHPICMTEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGN 174
Query: 182 GVSHTVPIYEG 214
SH +P+ G
Sbjct: 175 AASHVIPVLNG 185
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 54.4 bits (125), Expect = 1e-08
Identities = 23/84 (27%), Positives = 47/84 (55%)
Frame = +2
Query: 2 PEEHPVLLTEAPLNPKANREKMTQIMFXTFNSPAMYVAIQAVLSLYASGRTTGIVLDSGD 181
P E+ +++TE NP++ R+++ + F + PA Y+ QAV +A+ ++T +++D G
Sbjct: 99 PTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIGS 158
Query: 182 GVSHTVPIYEGYALPHAILRLDLA 253
+ P+ +G + I + LA
Sbjct: 159 DNASVTPVVDGLIIRKGIFKQSLA 182
Score = 42.3 bits (95), Expect = 6e-05
Identities = 25/68 (36%), Positives = 33/68 (48%)
Frame = +1
Query: 304 SFTTTAEREIVRDIKEKLCYVALDLXQEMATAAASTSLEKSYELPDGQVITIGNERFRCP 483
S+ +R ++ + KE + V L A AS+ K +E PDG G ERFR
Sbjct: 243 SYEIFQKRRVIEEWKESVLDV---LDTPFDEAKASSRNPKPFEFPDGVTHKFGQERFRIS 299
Query: 484 EALFQPSF 507
E LF PSF
Sbjct: 300 EILFNPSF 307
Score = 31.5 bits (68), Expect = 0.11
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = +3
Query: 507 PGMESCGIHETVYNSIMKCDVDIR 578
P S G+HE VY SI+ CD ++R
Sbjct: 317 PPQGSVGLHELVYQSILACDSELR 340
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 47.2 bits (107), Expect = 2e-06
Identities = 35/106 (33%), Positives = 52/106 (49%), Gaps = 20/106 (18%)
Frame = +1
Query: 247 LGCRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDLXQEMATA--AASTSLE 420
+G + LT+YL ++++ R Y+ E IV +IKE +CYV+ + ++M + LE
Sbjct: 188 VGGKLLTNYLKEVISYRKYNMME--ETYIVNEIKESVCYVSQNFKEDMEICHEKPRSKLE 245
Query: 421 KSYELPD------------------GQVITIGNERFRCPEALFQPS 504
Y LPD QV+ + NERF PE LF PS
Sbjct: 246 ICYALPDYSTGKHGYIVRDINQKIEQQVLNLSNERFMIPELLFSPS 291
Score = 25.0 bits (52), Expect = 9.7
Identities = 9/30 (30%), Positives = 18/30 (60%)
Frame = +2
Query: 161 IVLDSGDGVSHTVPIYEGYALPHAILRLDL 250
+V+DSG +H +P+ + A+ R+D+
Sbjct: 159 LVIDSGYSFTHIIPVIDFSVQEQAVKRVDV 188
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 37.9 bits (84), Expect = 0.001
Identities = 24/80 (30%), Positives = 36/80 (45%)
Frame = +2
Query: 11 HPVLLTEAPLNPKANREKMTQIMFXTFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVS 190
+PV L +RE TQ F P +A + ++ LYA G G+V+D G +
Sbjct: 147 YPVCLVIPTYWSLYDRELATQFFFEECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKT 206
Query: 191 HTVPIYEGYALPHAILRLDL 250
PI +G + A +L L
Sbjct: 207 DITPILDGQIIFTATQQLPL 226
>SPBC17D11.06 |spp2|pri2|DNA primase large subunit Spp2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 459
Score = 28.7 bits (61), Expect = 0.79
Identities = 14/34 (41%), Positives = 19/34 (55%), Gaps = 3/34 (8%)
Frame = +3
Query: 309 HHYR*EGNRS*HQG---EALLCXPRLXAGDGHRC 401
H Y EGNR ++G + +L P+L GD H C
Sbjct: 353 HTYGLEGNRKNYKGYNCQQILTGPQLGPGDAHGC 386
>SPAC144.01 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 112
Score = 27.5 bits (58), Expect = 1.8
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = -3
Query: 306 RVTPLGEDLHEVVGQVATAKSRRR 235
RVTP E +HE++ Q K R+R
Sbjct: 69 RVTPSEEKIHEMINQKRETKKRKR 92
>SPCC306.05c |ins1||INSIG domain protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 281
Score = 27.5 bits (58), Expect = 1.8
Identities = 11/23 (47%), Positives = 13/23 (56%)
Frame = +3
Query: 192 TPYPSTKVTLCPTPSSVWTWLSR 260
T YP L P+ SS W W+SR
Sbjct: 156 TKYPLGAAPLRPSQSSKWQWISR 178
>SPAC1296.04 |mug65||spore wall assembly protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 26.6 bits (56), Expect = 3.2
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +3
Query: 204 STKVTLCPTPSSVWTW 251
STK CP PS W W
Sbjct: 52 STKTATCPDPSWEWAW 67
>SPBC18E5.09c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 128
Score = 26.2 bits (55), Expect = 4.2
Identities = 14/43 (32%), Positives = 22/43 (51%), Gaps = 1/43 (2%)
Frame = +1
Query: 358 CYVALDLXQEMATAAASTSLEKSYELPDGQVITIGNE-RFRCP 483
CYV+ L +E + + SL SY +PD + I + R+ P
Sbjct: 39 CYVSRPLTKERSCSHPYISLGSSYGIPDAENIEYPRDARYHSP 81
>SPBC2G5.06c |hmt2|cad1|sulfide-quinone
oxidoreductase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 459
Score = 25.4 bits (53), Expect = 7.4
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = -1
Query: 569 DVALHDGVVHGLVDAARFHTRKEGW 495
D +L G + G+VD A++H + GW
Sbjct: 63 DTSLKPGEI-GIVDGAKYHYYQPGW 86
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,383,259
Number of Sequences: 5004
Number of extensions: 43655
Number of successful extensions: 159
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 154
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 301829700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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