Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0317
(660 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 122 3e-29
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 121 8e-29
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 61 1e-10
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 54 1e-08
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 29 0.45
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 29 0.59
SPBC16E9.02c |||CUE domain protein Cue5 |Schizosaccharomyces pom... 28 1.0
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 5.5
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 5.5
SPCC1620.14c |snf22|SPCC830.01c|ATP-dependent DNA helicase Snf22... 25 9.7
SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces... 25 9.7
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 9.7
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 122 bits (295), Expect = 3e-29
Identities = 51/83 (61%), Positives = 68/83 (81%)
Frame = +2
Query: 257 HPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXX 436
HPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RIR++AD C+GLQGFL+FH
Sbjct: 92 HPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGS 151
Query: 437 XXXXLLMERLSVDYGKKSKLEFA 505
LL+ERL+++YGKKS L+F+
Sbjct: 152 GLGALLLERLNMEYGKKSNLQFS 174
Score = 107 bits (256), Expect = 2e-24
Identities = 68/160 (42%), Positives = 88/160 (55%), Gaps = 6/160 (3%)
Frame = +3
Query: 198 VDLEPTVVDEVRTGTYRQLFIQNNLLLVRKMRXXXXXXXXXXLERK------S*IWFWTE 359
VDLEP V+D+VRTG Y+ LF ++ ++ + ++ I +
Sbjct: 72 VDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMAD 131
Query: 360 SASSLTSVPVCKDS*SSTPSVEVPALGSLPY*WSVSPLTTARSLNWSSHLPAPQVSTAVV 539
+ S L V T S LG+L +L +S + PAPQVST+VV
Sbjct: 132 NCSGLQGFLVFHSFGGGTGS----GLGALLLERLNMEYGKKSNLQFSVY-PAPQVSTSVV 186
Query: 540 EPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERP 659
EPYNS+LTTH TL++SDC FMVDNEA YDICRRNLDIERP
Sbjct: 187 EPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERP 226
Score = 95.1 bits (226), Expect = 8e-21
Identities = 48/68 (70%), Positives = 50/68 (73%), Gaps = 5/68 (7%)
Frame = +1
Query: 1 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSET 165
RE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSET
Sbjct: 2 REVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 166 GAGKHVPR 189
G GK VPR
Sbjct: 61 GQGKFVPR 68
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 121 bits (292), Expect = 8e-29
Identities = 53/83 (63%), Positives = 67/83 (80%)
Frame = +2
Query: 257 HPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXX 436
HPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR++AD C+GLQGFL+FH
Sbjct: 88 HPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGS 147
Query: 437 XXXXLLMERLSVDYGKKSKLEFA 505
LL+ERL+++Y KKSKL+F+
Sbjct: 148 GFGALLLERLAMEYTKKSKLQFS 170
Score = 107 bits (258), Expect = 1e-24
Identities = 70/160 (43%), Positives = 85/160 (53%), Gaps = 6/160 (3%)
Frame = +3
Query: 198 VDLEPTVVDEVRTGTYRQLFIQNNLLLVRK------MRXXXXXXXXXXLERKS*IWFWTE 359
VDLEP V+D+VRTG YR LF L+ ++ R E I +
Sbjct: 68 VDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIAD 127
Query: 360 SASSLTSVPVCKDS*SSTPSVEVPALGSLPY*WSVSPLTTARSLNWSSHLPAPQVSTAVV 539
+ S L V T S G+L T L +S + PAPQVST+VV
Sbjct: 128 NCSGLQGFLVFHSFGGGTGS----GFGALLLERLAMEYTKKSKLQFSVY-PAPQVSTSVV 182
Query: 540 EPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERP 659
EPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLDIERP
Sbjct: 183 EPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERP 222
Score = 102 bits (244), Expect = 5e-23
Identities = 44/63 (69%), Positives = 49/63 (77%)
Frame = +1
Query: 1 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 180
RE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK+
Sbjct: 2 REIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKY 61
Query: 181 VPR 189
VPR
Sbjct: 62 VPR 64
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 61.3 bits (142), Expect = 1e-10
Identities = 28/82 (34%), Positives = 44/82 (53%)
Frame = +2
Query: 260 PEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXX 439
P+ +I G+ A N +A+GHYT G E+ D VLD +R+ A+ C LQGF + H
Sbjct: 87 PDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTGSG 146
Query: 440 XXXLLMERLSVDYGKKSKLEFA 505
LL+ ++ +Y + F+
Sbjct: 147 MGTLLLSKIREEYPDRMMATFS 168
Score = 52.4 bits (120), Expect = 6e-08
Identities = 24/50 (48%), Positives = 32/50 (64%)
Frame = +3
Query: 510 PAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERP 659
PAP+ S VVEPYN+ L+ H +E+SD F +DNEA+ I L I+ P
Sbjct: 171 PAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSP 220
Score = 44.4 bits (100), Expect = 1e-05
Identities = 22/63 (34%), Positives = 30/63 (47%)
Frame = +1
Query: 1 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 180
RE + + GQ G Q+G A W EHG+ G T + N +F+E GK+
Sbjct: 2 REIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKY 59
Query: 181 VPR 189
VPR
Sbjct: 60 VPR 62
Score = 29.9 bits (64), Expect = 0.34
Identities = 12/27 (44%), Positives = 19/27 (70%)
Frame = +3
Query: 195 LVDLEPTVVDEVRTGTYRQLFIQNNLL 275
LVDLEP +D V++G + LF +N++
Sbjct: 65 LVDLEPGTMDAVKSGKFGNLFRPDNII 91
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 54.4 bits (125), Expect = 1e-08
Identities = 26/63 (41%), Positives = 38/63 (60%)
Frame = +1
Query: 1 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 180
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 181 VPR 189
+PR
Sbjct: 61 IPR 63
Score = 41.1 bits (92), Expect = 1e-04
Identities = 25/82 (30%), Positives = 41/82 (50%), Gaps = 2/82 (2%)
Frame = +2
Query: 248 TVVHPEQLITGKED--AANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXX 421
++ +PE ++ K A NN+A G Y+ + I + ++D I + AD L+GF + H
Sbjct: 84 SLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIA 142
Query: 422 XXXXXXXXXLLMERLSVDYGKK 487
L+ERL+ Y KK
Sbjct: 143 GGTGSGLGSFLLERLNDRYPKK 164
Score = 33.5 bits (73), Expect = 0.028
Identities = 16/46 (34%), Positives = 24/46 (52%)
Frame = +3
Query: 522 VSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERP 659
VS VV+PYNS+L ++D ++DN A+ I L + P
Sbjct: 178 VSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNP 223
Score = 31.1 bits (67), Expect = 0.15
Identities = 12/30 (40%), Positives = 20/30 (66%)
Frame = +3
Query: 195 LVDLEPTVVDEVRTGTYRQLFIQNNLLLVR 284
L+DLEP VV+ + + TY L+ N+L+ +
Sbjct: 66 LIDLEPRVVNNILSDTYGSLYNPENILITK 95
>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 674
Score = 29.5 bits (63), Expect = 0.45
Identities = 18/65 (27%), Positives = 36/65 (55%)
Frame = -1
Query: 198 RDSTGYVLASSGLAEESVERIVSTPDGLVCGHLAIRLDAVLQAVKLPAGITDLDSGLANV 19
+DS+ V SSGL + +E +V+ + +A R +A+ + + TD++S L ++
Sbjct: 538 KDSSITVAGSSGLTDSEIEAMVADAEKYRASDMA-RKEAIENGNRAESVCTDIESNL-DI 595
Query: 18 YRDAL 4
++D L
Sbjct: 596 HKDKL 600
>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1024
Score = 29.1 bits (62), Expect = 0.59
Identities = 13/49 (26%), Positives = 23/49 (46%)
Frame = -2
Query: 266 VLDEQLSVCASADLINNSRFKIDETARGTCLPAPVSLKKVLKESSPPPM 120
+++ +L + AD+IN SR+ ID + T + L P P+
Sbjct: 444 LVESKLQNRSFADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQPI 492
>SPBC16E9.02c |||CUE domain protein Cue5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 569
Score = 28.3 bits (60), Expect = 1.0
Identities = 17/66 (25%), Positives = 32/66 (48%)
Frame = +3
Query: 459 SVSPLTTARSLNWSSHLPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRR 638
S SP+ SLN + S A VE + IL ++ +E ++ AF +D+ +
Sbjct: 252 SSSPMMRRSSLNSNRRSVESSSSAAFVEGQSFILDSNGAIEVANSAFALDDSDLESAYNE 311
Query: 639 NLDIER 656
L++++
Sbjct: 312 ELEMKK 317
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 5.5
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +2
Query: 77 STASSLMARCPQTRPSGVETILSTLSSARPELAST 181
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 5.5
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 96 WPDAHRQDHRGWRRFFQHFLQR 161
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPCC1620.14c |snf22|SPCC830.01c|ATP-dependent DNA helicase
Snf22|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1680
Score = 25.0 bits (52), Expect = 9.7
Identities = 13/40 (32%), Positives = 23/40 (57%)
Frame = +3
Query: 405 SSTPSVEVPALGSLPY*WSVSPLTTARSLNWSSHLPAPQV 524
S +PSV+ + S + +SP +A +L SH+P+ +V
Sbjct: 385 SRSPSVDPNTVKSAQHIPRMSPSPSASALKTQSHVPSAKV 424
>SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1639
Score = 25.0 bits (52), Expect = 9.7
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +3
Query: 465 SPLTTARSLNWSSHLPAPQVSTAVVEPYNSILTTHTTLEHS 587
SP + SLN H PAP VS+ N++ +T+ HS
Sbjct: 116 SPQKSRESLNSLCHSPAPSVSSC----GNALNNDNTSASHS 152
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.0 bits (52), Expect = 9.7
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = +2
Query: 20 TLAKPESRSVMPAGSFTAWSTASSLMARCP 109
+L+ P SR++ P S + STASSL P
Sbjct: 181 SLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,748,644
Number of Sequences: 5004
Number of extensions: 56833
Number of successful extensions: 190
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 171
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 188
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 299817502
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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