Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0309
(576 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 36 0.006
SPBP23A10.11c |||conserved fungal protein|Schizosaccharomyces po... 28 0.85
SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pomb... 27 2.0
SPAC4F10.13c |mpd2||GYF domain|Schizosaccharomyces pombe|chr 1||... 27 2.0
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 27 2.6
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 25 6.0
SPAC15E1.10 ||SPAP7G5.01|PI31 proteasome regulator related|Schiz... 25 6.0
SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual 25 6.0
SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc... 25 7.9
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 25 7.9
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 35.5 bits (78), Expect = 0.006
Identities = 35/129 (27%), Positives = 55/129 (42%), Gaps = 4/129 (3%)
Frame = -2
Query: 491 TMSNAVKNTEKTTVGVDCLHISSCVTVQGLVVTTPPGPSRIQTGNPVGSATSL---SSSV 321
T S +V T T V L ++C T + T+ P S T ++TS+ S+ V
Sbjct: 451 TTSTSVPYTS-TPVTSTPLTTTNCTTSTSIPYTSTPVTSTPLTTTNCTTSTSVPYTSTPV 509
Query: 320 TDNVRAQAEANQVDSTPIAASVLMSQTKKLPRNCPTLGTPL-TAVLYRPLARPRQSTTIT 144
T + + + V STP+ + + T L + P TPL T + P ST +T
Sbjct: 510 TSSNYTISSSTPVTSTPVTTTNCTTSTSVLYTSTPVTSTPLATTNCTTSTSVPYTSTPVT 569
Query: 143 LQSETASKA 117
+ T S +
Sbjct: 570 SSNYTISSS 578
Score = 29.5 bits (63), Expect = 0.37
Identities = 21/84 (25%), Positives = 34/84 (40%), Gaps = 1/84 (1%)
Frame = -2
Query: 365 TGNPVGSATSLSSSVTDNVRAQAEANQVDSTPIAASVLMSQTKKLPRNCPTLGTPLTAV- 189
T PV S +++ T + + V STP+ + + T + P TPLT
Sbjct: 436 TSTPVTSTPLATTNCTTSTSVPYTSTPVTSTPLTTTNCTTSTSIPYTSTPVTSTPLTTTN 495
Query: 188 LYRPLARPRQSTTITLQSETASKA 117
+ P ST +T + T S +
Sbjct: 496 CTTSTSVPYTSTPVTSSNYTISSS 519
Score = 29.1 bits (62), Expect = 0.49
Identities = 25/97 (25%), Positives = 39/97 (40%), Gaps = 2/97 (2%)
Frame = -2
Query: 428 SSCVTVQGLVVTTPPGPSRIQTGNPVGSATSLSSS-VTDNVRAQAEANQVDSTPIAASVL 252
+SC T + T T P S+T L+++ T + + V STP+A +
Sbjct: 391 TSCTTSTSIPPTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNC 450
Query: 251 MSQTKKLPRNCPTLGTPLTAV-LYRPLARPRQSTTIT 144
+ T + P TPLT + P ST +T
Sbjct: 451 TTSTSVPYTSTPVTSTPLTTTNCTTSTSIPYTSTPVT 487
Score = 27.9 bits (59), Expect = 1.1
Identities = 22/82 (26%), Positives = 28/82 (34%)
Frame = -2
Query: 395 TTPPGPSRIQTGNPVGSATSLSSSVTDNVRAQAEANQVDSTPIAASVLMSQTKKLPRNCP 216
T PP S TG S S+S T + N +TP+ +V + T P
Sbjct: 315 TPPPPASTSSTGTSSSPLPSTSTSCTTSTSIPPTGNS--TTPVTPTVPPTSTSSTSTPPP 372
Query: 215 TLGTPLTAVLYRPLARPRQSTT 150
T T PL S T
Sbjct: 373 PASTSSTGTSSSPLLSTSTSCT 394
Score = 26.2 bits (55), Expect = 3.4
Identities = 23/109 (21%), Positives = 43/109 (39%), Gaps = 1/109 (0%)
Frame = -2
Query: 551 CESSTNSATRLGFLHRWVKVTMSNAVKNTEKTTVGVDCLHISSCVTVQGLVVTTPPGPSR 372
C +ST+ G +T + +T T++ + SS T + TT +
Sbjct: 115 CTTSTSIPPTGGSSSLSTPITPTVPPTSTSSTSIPIPPTSTSSTDTNSNPLPTTSTSCTT 174
Query: 371 IQTGNPVGSATSLSSSVTDNV-RAQAEANQVDSTPIAASVLMSQTKKLP 228
+ P G ++SLS+ +T V + + P + S + + LP
Sbjct: 175 STSIPPTGGSSSLSTPITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLP 223
>SPBP23A10.11c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 507
Score = 28.3 bits (60), Expect = 0.85
Identities = 21/56 (37%), Positives = 27/56 (48%)
Frame = -2
Query: 413 VQGLVVTTPPGPSRIQTGNPVGSATSLSSSVTDNVRAQAEANQVDSTPIAASVLMS 246
V G TTP + + + S SLSS+ TDN+ A V T +AASV S
Sbjct: 146 VYGDSATTPAASEAQASSDILSSLASLSSASTDNLAA-----SVTPTTVAASVASS 196
>SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 262
Score = 27.1 bits (57), Expect = 2.0
Identities = 13/53 (24%), Positives = 27/53 (50%)
Frame = -2
Query: 392 TPPGPSRIQTGNPVGSATSLSSSVTDNVRAQAEANQVDSTPIAASVLMSQTKK 234
TP S + + S+T +SSS+T ++ + A ++ S+ ++ + S K
Sbjct: 148 TPSSSSTTSSSSSSSSSTPISSSITSSISSSASSSVSSSSASSSGSISSADAK 200
>SPAC4F10.13c |mpd2||GYF domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 992
Score = 27.1 bits (57), Expect = 2.0
Identities = 22/87 (25%), Positives = 39/87 (44%), Gaps = 8/87 (9%)
Frame = -2
Query: 383 GPSRIQTGN--PVGSATSLSSS------VTDNVRAQAEANQVDSTPIAASVLMSQTKKLP 228
GPSR T + PVG++ S+ + + D +A+A + S P+A S SQ +
Sbjct: 297 GPSRFPTNSNVPVGNSMSIRDTDSPLNILVDKAKAKASIKENASQPVAPSA--SQREHSA 354
Query: 227 RNCPTLGTPLTAVLYRPLARPRQSTTI 147
N P + ++ A P+ ++
Sbjct: 355 VNSPAAAMSPSTAMFSSEAFPQHLASL 381
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 26.6 bits (56), Expect = 2.6
Identities = 25/63 (39%), Positives = 29/63 (46%)
Frame = -3
Query: 490 P*AMRSKIPRRPPSVWIAST*APALRFRAWLLPPHLGPAGSKPVTLLGLPPACRPALPTT 311
P A S P PPS+ + + AP L A + PP PAG LPPA PA P
Sbjct: 425 PSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPL--PAGMPAAP--PLPPAA-PAPPPA 479
Query: 310 CAP 302
AP
Sbjct: 480 PAP 482
Score = 25.4 bits (53), Expect = 6.0
Identities = 16/42 (38%), Positives = 21/42 (50%), Gaps = 6/42 (14%)
Frame = -3
Query: 394 PPHLGPA-GSKPVTL-----LGLPPACRPALPTTCAPKLKPI 287
PP P+ G +P L + PPA PA+P AP L P+
Sbjct: 365 PPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPL 406
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 25.4 bits (53), Expect = 6.0
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +3
Query: 501 PTVQKPQPGCRVSTALTVEPT 563
PTVQ PQP ++ TA +P+
Sbjct: 1307 PTVQHPQPTKQIPTAAVKDPS 1327
>SPAC15E1.10 ||SPAP7G5.01|PI31 proteasome regulator
related|Schizosaccharomyces pombe|chr 1|||Manual
Length = 265
Score = 25.4 bits (53), Expect = 6.0
Identities = 10/31 (32%), Positives = 17/31 (54%)
Frame = -2
Query: 542 STNSATRLGFLHRWVKVTMSNAVKNTEKTTV 450
S N +TRL F ++W+ + +N N T +
Sbjct: 58 SQNVSTRLIFWNKWIYILCANTSSNITATRI 88
>SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual
Length = 978
Score = 25.4 bits (53), Expect = 6.0
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = +1
Query: 13 NSIRNSNYNGNLPLFVIVHGWNSNGN 90
+SIRNS++N L LF V S+GN
Sbjct: 604 SSIRNSDFNSQLYLFETVGVLISSGN 629
>SPBC16H5.10c |prp43||ATP-dependent RNA helicase
Prp43|Schizosaccharomyces pombe|chr 2|||Manual
Length = 735
Score = 25.0 bits (52), Expect = 7.9
Identities = 15/49 (30%), Positives = 21/49 (42%)
Frame = -2
Query: 419 VTVQGLVVTTPPGPSRIQTGNPVGSATSLSSSVTDNVRAQAEANQVDST 273
+T+ G+V PG S+ + NP SL S AQ A + T
Sbjct: 365 LTIDGIVYVVDPGFSKQKIYNPRIRVESLLVSPISKASAQQRAGRAGRT 413
>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1036
Score = 25.0 bits (52), Expect = 7.9
Identities = 23/77 (29%), Positives = 33/77 (42%)
Frame = -2
Query: 359 NPVGSATSLSSSVTDNVRAQAEANQVDSTPIAASVLMSQTKKLPRNCPTLGTPLTAVLYR 180
N SAT+ SSS++ V + +ST A S + + N +L P +
Sbjct: 233 NSTTSATATSSSISSTVSSSTPLTSSNSTTAATSA-SATSSSAQYNTSSL-LPSSTPSST 290
Query: 179 PLARPRQSTTITLQSET 129
PL+ STT T S T
Sbjct: 291 PLS-SANSTTATSASST 306
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,581,476
Number of Sequences: 5004
Number of extensions: 57249
Number of successful extensions: 199
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 198
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 246098644
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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