BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0307
(646 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC106.02c |srx1||sulfiredoxin|Schizosaccharomyces pombe|chr 2|... 59 6e-10
SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomy... 27 2.3
SPAC823.15 |ppa1||minor serine/threonine protein phosphatase Ppa... 27 3.1
SPBC16C6.09 |ogm4|oma4|protein O-mannosyltransferase Ogm4|Schizo... 25 7.1
SPAC11H11.03c |||SMR and DUF1771 domain protein|Schizosaccharomy... 25 7.1
SPAC6F6.06c |rax2||cell polarity factor Rax2|Schizosaccharomyces... 25 7.1
SPAC631.02 |||bromodomain protein|Schizosaccharomyces pombe|chr ... 25 9.3
>SPBC106.02c |srx1||sulfiredoxin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 124
Score = 58.8 bits (136), Expect = 6e-10
Identities = 25/60 (41%), Positives = 37/60 (61%)
Frame = +1
Query: 334 AGNVPPIDILWIKGSQGGDYYYSFGGCHRFAAYKRLNRPTIPAKLIKSTVSDLKTYLGTA 513
AG +PP+D+L K S G YY++FGGCHR A+ R + KL+ + + L+ YLG +
Sbjct: 58 AGELPPVDVLTFKKS-GKPYYFAFGGCHRLRAHDEAGRKKVRCKLVNCSPNTLRLYLGAS 116
Score = 38.3 bits (85), Expect = 0.001
Identities = 17/40 (42%), Positives = 25/40 (62%)
Frame = +2
Query: 71 MTSVHSAYKEEVHDVPMSVITRPFIPELDEAKVESLMETI 190
MTS+H+ + ++ MS + RP P LD KV S+MET+
Sbjct: 1 MTSIHTGSNNNIVELDMSELIRPIPPVLDMNKVNSMMETM 40
>SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 786
Score = 27.1 bits (57), Expect = 2.3
Identities = 23/79 (29%), Positives = 36/79 (45%), Gaps = 5/79 (6%)
Frame = +3
Query: 306 FCSHN*ARASRQRPANRHTLDQRLSRW-----RLLLQFRRMPQIRSL*TFESSHDSRQTD 470
F HN + P LDQ + ++ R L+ FR P +R L + + R+ D
Sbjct: 382 FVDHNTKTTTWDDPRLPSALDQDVPQYKCDFRRKLIYFRSQPGMRPL-PGQCNVKVRR-D 439
Query: 471 QIYSFRFKDLSRYSTPDLK 527
I+ + ++ RYS DLK
Sbjct: 440 HIFEDSYAEIMRYSAHDLK 458
>SPAC823.15 |ppa1||minor serine/threonine protein phosphatase
Ppa1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 309
Score = 26.6 bits (56), Expect = 3.1
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +3
Query: 390 LLLQFR-RMPQIRSL*TFESSHDSRQTDQIYSFRFKDLSRYSTPDL 524
LL+ F+ R PQ ++ +H+SRQ Q+Y F + L +Y ++
Sbjct: 99 LLIAFKIRYPQRITI--LRGNHESRQITQVYGFYDECLRKYGNANV 142
>SPBC16C6.09 |ogm4|oma4|protein O-mannosyltransferase
Ogm4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 778
Score = 25.4 bits (53), Expect = 7.1
Identities = 12/21 (57%), Positives = 12/21 (57%), Gaps = 1/21 (4%)
Frame = +3
Query: 141 SFRSWTRPKWNLSWK-QYSKQ 200
S WTR KW SW QY KQ
Sbjct: 757 SVDEWTRRKWLDSWVFQYQKQ 777
>SPAC11H11.03c |||SMR and DUF1771 domain protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 206
Score = 25.4 bits (53), Expect = 7.1
Identities = 17/56 (30%), Positives = 24/56 (42%)
Frame = +1
Query: 73 DKCTQRLQGRSP*CSNVGYHQALHSGAGRGQSGISHGNNTVSNVVTALNPRSHKYD 240
D+ Q +Q R C G + LH GRG +H +V L +S KY+
Sbjct: 83 DEAVQAVQQRIENCIRRGDNH-LHIIVGRGNHSANHVEKLRPAIVRMLEQQSIKYN 137
>SPAC6F6.06c |rax2||cell polarity factor Rax2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1155
Score = 25.4 bits (53), Expect = 7.1
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = +3
Query: 210 SAKSTFTQIRRWECLKNHSTIDH 278
S K +FT I RW ++ TI+H
Sbjct: 144 SDKPSFTSIYRWNVTNSNVTIEH 166
>SPAC631.02 |||bromodomain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 727
Score = 25.0 bits (52), Expect = 9.3
Identities = 11/29 (37%), Positives = 17/29 (58%)
Frame = +1
Query: 166 SGISHGNNTVSNVVTALNPRSHKYDDGNA 252
SGI+ NTV+ + A N +S DD ++
Sbjct: 686 SGIAQNKNTVTGNIAAYNTKSLGSDDSSS 714
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,801,198
Number of Sequences: 5004
Number of extensions: 59261
Number of successful extensions: 151
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 147
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 150
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 289756512
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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