Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0305
(581 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_43840| Best HMM Match : NDK (HMM E-Value=0) 124 5e-29
SB_45281| Best HMM Match : No HMM Matches (HMM E-Value=.) 42 3e-04
SB_10572| Best HMM Match : Glyco_hydro_12 (HMM E-Value=3) 29 2.8
SB_57396| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 2.8
SB_13421| Best HMM Match : Harpin (HMM E-Value=0.62) 28 4.8
>SB_43840| Best HMM Match : NDK (HMM E-Value=0)
Length = 786
Score = 124 bits (299), Expect = 5e-29
Identities = 55/80 (68%), Positives = 62/80 (77%)
Frame = +1
Query: 256 VVPMVWEGLNVVKTGRQMLGATNPADSQPGTIRGDLCIQVGRNIIHGSDSVESAKKEIGL 435
V MVWEGL VVKT R MLG T+PA S PGTIRGD I +GRNIIHGSD+VE+AK+EI L
Sbjct: 707 VCAMVWEGLGVVKTARVMLGETDPAKSLPGTIRGDFSIHIGRNIIHGSDAVETAKEEIAL 766
Query: 436 WFTDKEVVGWTPANENWVYE 495
WF D E+V WTP N W+YE
Sbjct: 767 WFKDDELVDWTPCNNPWMYE 786
Score = 110 bits (265), Expect = 7e-25
Identities = 52/67 (77%), Positives = 59/67 (88%)
Frame = +2
Query: 53 ERTFIMVKPDGVQRGLVGTIIERFEKKGFKLVGLKFVWPSEELLQQHYSDLASRPFFPGL 232
ERTFIMVKPDGVQRGLVG II+RFE+KGFKLV LK V SEE L++HY+DLA PF+PGL
Sbjct: 127 ERTFIMVKPDGVQRGLVGEIIKRFEQKGFKLVALKMVQESEEHLKKHYADLAHLPFYPGL 186
Query: 233 VKYMSSG 253
VK+MSSG
Sbjct: 187 VKFMSSG 193
Score = 91.1 bits (216), Expect = 6e-19
Identities = 41/65 (63%), Positives = 49/65 (75%)
Frame = +2
Query: 53 ERTFIMVKPDGVQRGLVGTIIERFEKKGFKLVGLKFVWPSEELLQQHYSDLASRPFFPGL 232
ERTF+M+KPD V RGL+G II RFEKKGFKLV +KFV SE+ ++HY LA F+ GL
Sbjct: 639 ERTFLMIKPDAVSRGLIGEIISRFEKKGFKLVAMKFVKKSEDHFRKHYESLAKLKFYDGL 698
Query: 233 VKYMS 247
KYMS
Sbjct: 699 CKYMS 703
Score = 34.3 bits (75), Expect = 0.074
Identities = 12/17 (70%), Positives = 15/17 (88%)
Frame = +1
Query: 328 ADSQPGTIRGDLCIQVG 378
ADS+PGTIRGD C+ +G
Sbjct: 199 ADSKPGTIRGDFCVHIG 215
>SB_45281| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1256
Score = 42.3 bits (95), Expect = 3e-04
Identities = 25/67 (37%), Positives = 37/67 (55%)
Frame = +2
Query: 53 ERTFIMVKPDGVQRGLVGTIIERFEKKGFKLVGLKFVWPSEELLQQHYSDLASRPFFPGL 232
+ T ++KP+ V+ I +R ++ GFK+ K V S+EL Q Y + + FF GL
Sbjct: 673 QSTVAVIKPE-VEPDQRELIKQRIKEAGFKIQLQKEVTLSKELASQFYHEHEGKDFFEGL 731
Query: 233 VKYMSSG 253
YMSSG
Sbjct: 732 TDYMSSG 738
Score = 39.5 bits (88), Expect = 0.002
Identities = 21/69 (30%), Positives = 39/69 (56%)
Frame = +2
Query: 47 QRERTFIMVKPDGVQRGLVGTIIERFEKKGFKLVGLKFVWPSEELLQQHYSDLASRPFFP 226
Q +RT +++PD + R +I+ + ++ GF++ K + + E ++ YS+ + FF
Sbjct: 536 QLQRTLALIRPDAL-RSRRESIMSKIQEAGFEIAMSKEMHLTREQAEEFYSEHKDQEFFD 594
Query: 227 GLVKYMSSG 253
LV MSSG
Sbjct: 595 TLVTNMSSG 603
Score = 31.9 bits (69), Expect = 0.39
Identities = 19/68 (27%), Positives = 34/68 (50%), Gaps = 3/68 (4%)
Frame = +1
Query: 265 MVWEGLNVVKTGRQMLGATNPADSQ---PGTIRGDLCIQVGRNIIHGSDSVESAKKEIGL 435
MV + V R ++G +P ++ P +IR L V +N++HG E A K I
Sbjct: 743 MVLSKEDAVSGWRSLMGPVDPEQAKEMAPESIRAALGKDVMKNVVHGPSDPEKAGKVIKE 802
Query: 436 WFTDKEVV 459
+F + +++
Sbjct: 803 FFPEAKIL 810
Score = 29.9 bits (64), Expect = 1.6
Identities = 19/63 (30%), Positives = 32/63 (50%), Gaps = 4/63 (6%)
Frame = +1
Query: 283 NVVKTGRQMLG---ATNPADSQPGTIRGDLCIQVGR-NIIHGSDSVESAKKEIGLWFTDK 450
+ ++ R MLG D P ++R ++ N +HGSD+ E+A+KEI +F +
Sbjct: 614 DAIEGWRGMLGPKEVEKAKDEAPESLRAQFQVEDSPINPLHGSDTAENAEKEIQKFFPMQ 673
Query: 451 EVV 459
V
Sbjct: 674 STV 676
Score = 28.7 bits (61), Expect = 3.7
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = +1
Query: 382 NIIHGSDSVESAKKEIGLWFTD 447
N +HG DS ESA +E+ +F D
Sbjct: 501 NAVHGCDSNESAARELAFFFPD 522
>SB_10572| Best HMM Match : Glyco_hydro_12 (HMM E-Value=3)
Length = 294
Score = 29.1 bits (62), Expect = 2.8
Identities = 9/25 (36%), Positives = 15/25 (60%)
Frame = +2
Query: 209 SRPFFPGLVKYMSSGLWSLWYGRAL 283
+ P PG+ K++ SG W +W G +
Sbjct: 200 NNPILPGVPKFLISGGWPMWCGNEI 224
>SB_57396| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 757
Score = 29.1 bits (62), Expect = 2.8
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +2
Query: 455 LWAGHLQMKTGFMSKFILSVYFVLFS*FMLNIL 553
LW GHL++ G +LS + +L F+LN+L
Sbjct: 296 LWRGHLKVIQGKFGSGVLSYFVLLRWLFLLNVL 328
>SB_13421| Best HMM Match : Harpin (HMM E-Value=0.62)
Length = 375
Score = 28.3 bits (60), Expect = 4.8
Identities = 19/50 (38%), Positives = 22/50 (44%), Gaps = 2/50 (4%)
Frame = -3
Query: 339 LRVSWVSCAKHLTASL--HNIKALPYHRDHSPELMYFTRPGKKGRDAKSL 196
LRVSW S H ASL H +L H SP+ P G+ SL
Sbjct: 226 LRVSWASLKSHGAASLKSHEAASLKSHVAASPKSHGAASPSLMGQHFSSL 275
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,246,408
Number of Sequences: 59808
Number of extensions: 377216
Number of successful extensions: 1061
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 983
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1061
length of database: 16,821,457
effective HSP length: 78
effective length of database: 12,156,433
effective search space used: 1397989795
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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