Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0298
         (746 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g23250.1 68418.m02720 succinyl-CoA ligase [GDP-forming] alpha...    78   5e-15
At5g08300.1 68418.m00977 succinyl-CoA ligase [GDP-forming] alpha...    77   1e-14
At3g06650.1 68416.m00774 ATP-citrate synthase, putative / ATP-ci...    33   0.15 
At5g49460.1 68418.m06119 ATP-citrate synthase, putative / ATP-ci...    31   0.61 
At1g09730.1 68414.m01092 Ulp1 protease family protein low simila...    27   10.0 

>At5g23250.1 68418.m02720 succinyl-CoA ligase [GDP-forming]
           alpha-chain, mitochondrial, putative / succinyl-CoA
           synthetase, alpha chain, putative / SCS-alpha, putative
           similar to SP|P36967 Succinyl-CoA ligase [GDP-forming]
           alpha-chain, mitochondrial precursor (EC 6.2.1.4)
           (Succinyl-CoA synthetase, alpha chain) (SCS-alpha)
           {Dictyostelium discoideum}; contains Pfam profiles
           PF00549: CoA-ligase, PF02629: CoA binding domain
          Length = 341

 Score = 78.2 bits (184), Expect = 5e-15
 Identities = 43/83 (51%), Positives = 51/83 (61%)
 Frame = +2

Query: 2   FLKDPETKGIILIGEIGGNAEELASEYLTQYNTGQKAKPVVSFIAGLTAPPGRRMXXXXX 181
           F  DP+T+GI+LIGEIGG AEE A+  + +  T    KPVV+FIAGLTAPPGRRM     
Sbjct: 240 FFVDPQTEGIVLIGEIGGTAEEDAAALIKENGTD---KPVVAFIAGLTAPPGRRMGHAGA 296

Query: 182 XXXXXXXXXMDKIKALEKANVIV 250
                     DKIK+L  A V V
Sbjct: 297 IVSGGKGTAQDKIKSLRDAGVKV 319


>At5g08300.1 68418.m00977 succinyl-CoA ligase [GDP-forming]
           alpha-chain, mitochondrial, putative / succinyl-CoA
           synthetase, alpha chain, putative / SCS-alpha, putative
           identical to SP|P53586 Succinyl-CoA ligase [GDP-forming]
           alpha-chain, mitochondrial precursor (EC 6.2.1.4)
           (Succinyl-CoA synthetase, alpha chain) (SCS-alpha)
           {Arabidopsis thaliana}; strong similarity to SP|P13086
           Succinyl-CoA ligase [GDP-forming] alpha-chain,
           mitochondrial precursor {Rattus norvegicus}; contains
           Pfam profiles PF00549: CoA-ligase, PF02629: CoA binding
           domain
          Length = 347

 Score = 77.0 bits (181), Expect = 1e-14
 Identities = 43/83 (51%), Positives = 50/83 (60%)
 Frame = +2

Query: 2   FLKDPETKGIILIGEIGGNAEELASEYLTQYNTGQKAKPVVSFIAGLTAPPGRRMXXXXX 181
           F  DP+T+GI+LIGEIGG AEE A+  +    T    KPVV+FIAGLTAPPGRRM     
Sbjct: 245 FFVDPQTEGIVLIGEIGGTAEEDAAALIKASGT---EKPVVAFIAGLTAPPGRRMGHAGA 301

Query: 182 XXXXXXXXXMDKIKALEKANVIV 250
                     DKIK+L  A V V
Sbjct: 302 IVSGGKGTAQDKIKSLNDAGVKV 324


>At3g06650.1 68416.m00774 ATP-citrate synthase, putative /
           ATP-citrate (pro-S-)-lyase, putative / citrate cleavage
           enzyme, putative strong similarity to ATP:citrate lyase
           [Capsicum annuum] GI:13160653; contains Pfam profiles
           PF00549: CoA-ligase, PF02629: CoA binding domain
          Length = 608

 Score = 33.5 bits (73), Expect = 0.15
 Identities = 16/45 (35%), Positives = 28/45 (62%)
 Frame = +2

Query: 14  PETKGIILIGEIGGNAEELASEYLTQYNTGQKAKPVVSFIAGLTA 148
           P+ K ++++GE+GG  E    E + Q   G+  KPVV++++G  A
Sbjct: 221 PQIKMVVVLGELGGRDEYSLVEAMKQ---GKVTKPVVAWVSGTCA 262


>At5g49460.1 68418.m06119 ATP-citrate synthase, putative /
           ATP-citrate (pro-S-)-lyase, putative / citrate cleavage
           enzyme, putative strong similarity to ATP:citrate lyase
           [Capsicum annuum] GI:13160653; contains Pfam profiles
           PF00549: CoA-ligase, PF02629: CoA binding domain
          Length = 608

 Score = 31.5 bits (68), Expect = 0.61
 Identities = 16/45 (35%), Positives = 28/45 (62%)
 Frame = +2

Query: 14  PETKGIILIGEIGGNAEELASEYLTQYNTGQKAKPVVSFIAGLTA 148
           P+ K ++++GE+GG  E    E L +   G+  KPVV++++G  A
Sbjct: 221 PQIKMMVVLGELGGRDEYSLVEALKE---GKVNKPVVAWVSGTCA 262


>At1g09730.1 68414.m01092 Ulp1 protease family protein low
           similarity to SP|Q9GZR1 SUMO-1-specific protease 1 (EC
           3.4.22.-) (Sentrin-specific protease SENP6) (Protease
           FKSG6) {Homo sapiens}; contains Pfam profile PF02902:
           Ulp1 protease family, C-terminal catalytic domain
          Length = 984

 Score = 27.5 bits (58), Expect = 10.0
 Identities = 14/32 (43%), Positives = 20/32 (62%)
 Frame = -2

Query: 307 SLIVSFLCEVPRPFWPETSDDYISFF*SLNFV 212
           +L+ ++LCE  +    ETSDD  S F +L FV
Sbjct: 586 NLVQTYLCEEWKERHKETSDDISSRFMNLRFV 617


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,569,943
Number of Sequences: 28952
Number of extensions: 273094
Number of successful extensions: 533
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 525
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 531
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1653386488
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)