Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0294
         (779 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d...   118   1e-27
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase...   116   3e-27
SPCC70.08c |||methyltransferase |Schizosaccharomyces pombe|chr 3...    32   0.11 
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo...    29   0.99 
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1...    28   1.7  
SPBC18H10.19 |||human UVRAG |Schizosaccharomyces pombe|chr 2|||M...    27   4.0  
SPAC2F3.15 |lsk1||latrunculin sensitive kinase Lsk1 |Schizosacch...    27   4.0  
SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces ...    26   5.3  

>SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde
           dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 380

 Score =  118 bits (283), Expect = 1e-27
 Identities = 50/81 (61%), Positives = 62/81 (76%)
 Frame = +1

Query: 10  VRSTQGQGVMPDGTRRFRCKGQELYHFMGCSTFSQYTVVLEISLCKVAEAAPLDKVCLLG 189
           +R+TQG+G+MPDGT RF C G  L HFMGCSTFS+YTVV +IS+  +   APLD VCLLG
Sbjct: 119 IRTTQGKGLMPDGTSRFSCNGNTLLHFMGCSTFSEYTVVADISVVAIERLAPLDSVCLLG 178

Query: 190 CGVPTGYGAALNTAKVEPGSN 252
           CG+ TGYGAA  TA ++ G +
Sbjct: 179 CGITTGYGAATITADIKEGDS 199



 Score = 83.8 bits (198), Expect = 2e-17
 Identities = 35/63 (55%), Positives = 45/63 (71%)
 Frame = +3

Query: 321 RIIGVDINPDKFEVAKKFGVNEFVNPKDYDKPIQQVLVDLTDGGLEYTFECIGNVGTMRA 500
           RI G+D+NP+K   A  FG  +F+NP D   PIQ VL+  TDGGL++TF+C GNV  MR+
Sbjct: 223 RIFGIDVNPEKKNWAMSFGATDFINPNDLQSPIQDVLIHETDGGLDWTFDCTGNVHVMRS 282

Query: 501 ALE 509
           ALE
Sbjct: 283 ALE 285



 Score = 73.3 bits (172), Expect = 4e-14
 Identities = 30/39 (76%), Positives = 34/39 (87%)
 Frame = +2

Query: 497 SCTRACHKGWGVSVIIGVAAAGEEISTRPFQLVTGRTWK 613
           S   ACHKGWG S++IGVAAAG+EISTRPFQLVTGR W+
Sbjct: 282 SALEACHKGWGQSIVIGVAAAGQEISTRPFQLVTGRVWR 320



 Score = 54.4 bits (125), Expect = 2e-08
 Identities = 23/40 (57%), Positives = 29/40 (72%)
 Frame = +1

Query: 613 GTAFGGYKSRESVPKLVDEYLEKKLPLDEFVTHNVPLKEI 732
           G AFGG K R  +P LV EYL+ KL +D+++TH  PLKEI
Sbjct: 321 GCAFGGVKGRSQLPDLVKEYLDHKLEIDKYITHRRPLKEI 360


>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 378

 Score =  116 bits (280), Expect = 3e-27
 Identities = 49/81 (60%), Positives = 65/81 (80%)
 Frame = +1

Query: 7   EVRSTQGQGVMPDGTRRFRCKGQELYHFMGCSTFSQYTVVLEISLCKVAEAAPLDKVCLL 186
           ++R TQG+G+MPDGT RF C+ + L H+MGCS+FSQYTVV +ISL  ++ +APL  +CLL
Sbjct: 115 KIRETQGRGLMPDGTSRFSCRDKTLLHYMGCSSFSQYTVVADISLVAISHSAPLRSICLL 174

Query: 187 GCGVPTGYGAALNTAKVEPGS 249
           GCGV TG+GA  ++AKVE GS
Sbjct: 175 GCGVTTGFGAVTHSAKVESGS 195



 Score = 65.7 bits (153), Expect = 7e-12
 Identities = 29/64 (45%), Positives = 43/64 (67%)
 Frame = +3

Query: 318 NRIIGVDINPDKFEVAKKFGVNEFVNPKDYDKPIQQVLVDLTDGGLEYTFECIGNVGTMR 497
           +RII +DIN DK   AKKFG  +F++     K + Q ++D+TDGG++Y F+C GNV  M+
Sbjct: 219 SRIIAIDINADKEVYAKKFGATDFIDSSKV-KDLVQYVIDVTDGGVDYAFDCTGNVTVMQ 277

Query: 498 AALE 509
             L+
Sbjct: 278 QELQ 281



 Score = 49.2 bits (112), Expect = 7e-07
 Identities = 20/31 (64%), Positives = 24/31 (77%)
 Frame = +2

Query: 512 CHKGWGVSVIIGVAAAGEEISTRPFQLVTGR 604
           CHKGWG   +IGVAAAG+ +  RPF +VTGR
Sbjct: 283 CHKGWGKLCVIGVAAAGKTLDFRPFLVVTGR 313



 Score = 48.4 bits (110), Expect = 1e-06
 Identities = 19/40 (47%), Positives = 27/40 (67%)
 Frame = +1

Query: 613 GTAFGGYKSRESVPKLVDEYLEKKLPLDEFVTHNVPLKEI 732
           G+AFGG K R  +P  VDEY++    +DE++T+  PLK I
Sbjct: 317 GSAFGGVKGRSELPNFVDEYMQGHFKVDEYITNEEPLKNI 356


>SPCC70.08c |||methyltransferase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 260

 Score = 31.9 bits (69), Expect = 0.11
 Identities = 10/26 (38%), Positives = 19/26 (73%)
 Frame = +3

Query: 321 RIIGVDINPDKFEVAKKFGVNEFVNP 398
           R++G+D +PD  + A++ G+N +V P
Sbjct: 57  RVVGIDASPDMIKAARELGLNAYVIP 82


>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 2244

 Score = 28.7 bits (61), Expect = 0.99
 Identities = 15/42 (35%), Positives = 23/42 (54%)
 Frame = +1

Query: 580  SIPTCYRSHLEGTAFGGYKSRESVPKLVDEYLEKKLPLDEFV 705
            SIP+     +  TAF G+  RE++P L+    E +L L + V
Sbjct: 1750 SIPSRLAKFVGNTAFTGFGVREAIPLLLTAVNEGRLTLKDVV 1791


>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 800

 Score = 27.9 bits (59), Expect = 1.7
 Identities = 14/38 (36%), Positives = 20/38 (52%)
 Frame = -3

Query: 648 TFSTFVTSKSCSFQVRPVTSWNGRVLISSPAAATPMIT 535
           T +   TS S  +   PVTS N  +  S+P  +TP+ T
Sbjct: 492 TTTNCTTSTSVPYTSTPVTSSNYTISSSTPVTSTPVTT 529



 Score = 27.1 bits (57), Expect = 3.0
 Identities = 13/32 (40%), Positives = 18/32 (56%)
 Frame = -3

Query: 630 TSKSCSFQVRPVTSWNGRVLISSPAAATPMIT 535
           TS S  +   PVTS N  +  S+P  +TP+ T
Sbjct: 557 TSTSVPYTSTPVTSSNYTISSSTPVTSTPVTT 588


>SPBC18H10.19 |||human UVRAG |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 307

 Score = 26.6 bits (56), Expect = 4.0
 Identities = 14/38 (36%), Positives = 22/38 (57%)
 Frame = +1

Query: 634 KSRESVPKLVDEYLEKKLPLDEFVTHNVPLKEIMRHSI 747
           KS+ES+ K   EY+E    L   V  N+ ++ +M H+I
Sbjct: 113 KSKESLQKDEAEYVELANSLKTKVETNIEIRILMAHAI 150


>SPAC2F3.15 |lsk1||latrunculin sensitive kinase Lsk1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 593

 Score = 26.6 bits (56), Expect = 4.0
 Identities = 13/40 (32%), Positives = 23/40 (57%)
 Frame = -3

Query: 642 STFVTSKSCSFQVRPVTSWNGRVLISSPAAATPMITDTPH 523
           S+F  S   S  ++P +++NGR +   P+++ P I   PH
Sbjct: 162 SSFRRSPPSSVHMKP-SAFNGRKVSRRPSSSPPPIPSIPH 200


>SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces
            pombe|chr 3|||Manual
          Length = 1502

 Score = 26.2 bits (55), Expect = 5.3
 Identities = 11/24 (45%), Positives = 13/24 (54%)
 Frame = +1

Query: 139  LCKVAEAAPLDKVCLLGCGVPTGY 210
            +CKV E A L   CLL C   T +
Sbjct: 979  ICKVREIASLSLTCLLDCSKMTTF 1002


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,098,615
Number of Sequences: 5004
Number of extensions: 61627
Number of successful extensions: 171
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 160
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 170
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 377352472
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)