Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0186
(625 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 138 8e-34
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 138 8e-34
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 138 8e-34
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 51 1e-07
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 27 2.2
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 6.7
SPBC418.01c |his4|SPBC887.20c|imidazoleglycerol-phosphate syntha... 25 8.9
SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2... 25 8.9
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 138 bits (333), Expect = 8e-34
Identities = 59/83 (71%), Positives = 71/83 (85%)
Frame = +1
Query: 13 ADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSG 192
A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSG
Sbjct: 336 ASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSG 395
Query: 193 DAAIVNLVPSKPLCVESFQEFHP 261
DA I +VPSKP+CVE+F ++ P
Sbjct: 396 DACIAKMVPSKPMCVEAFTDYAP 418
Score = 46.8 bits (106), Expect = 3e-06
Identities = 22/22 (100%), Positives = 22/22 (100%)
Frame = +3
Query: 258 PLGRFAVRDMRQTVAVGVIKAV 323
PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 418 PLGRFAVRDMRQTVAVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 138 bits (333), Expect = 8e-34
Identities = 59/83 (71%), Positives = 71/83 (85%)
Frame = +1
Query: 13 ADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSG 192
A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSG
Sbjct: 336 ASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSG 395
Query: 193 DAAIVNLVPSKPLCVESFQEFHP 261
DA I +VPSKP+CVE+F ++ P
Sbjct: 396 DACIAKMVPSKPMCVEAFTDYAP 418
Score = 46.8 bits (106), Expect = 3e-06
Identities = 22/22 (100%), Positives = 22/22 (100%)
Frame = +3
Query: 258 PLGRFAVRDMRQTVAVGVIKAV 323
PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 418 PLGRFAVRDMRQTVAVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 138 bits (333), Expect = 8e-34
Identities = 59/83 (71%), Positives = 71/83 (85%)
Frame = +1
Query: 13 ADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSG 192
A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSG
Sbjct: 336 ASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSG 395
Query: 193 DAAIVNLVPSKPLCVESFQEFHP 261
DA I +VPSKP+CVE+F ++ P
Sbjct: 396 DACIAKMVPSKPMCVEAFTDYAP 418
Score = 46.8 bits (106), Expect = 3e-06
Identities = 22/22 (100%), Positives = 22/22 (100%)
Frame = +3
Query: 258 PLGRFAVRDMRQTVAVGVIKAV 323
PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 418 PLGRFAVRDMRQTVAVGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 51.2 bits (117), Expect = 1e-07
Identities = 25/84 (29%), Positives = 43/84 (51%)
Frame = +1
Query: 4 HEAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSI 183
H F AQ+ +L P ++ GY+ V+ HTA FA++ K+D +T + ++ P
Sbjct: 555 HATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHKLD-KTNRKSKKPPMFA 613
Query: 184 KSGDAAIVNLVPSKPLCVESFQEF 255
G I L P+C+E F+++
Sbjct: 614 TKGMKIIAELETQTPVCMERFEDY 637
Score = 25.4 bits (53), Expect = 6.7
Identities = 12/23 (52%), Positives = 17/23 (73%), Gaps = 1/23 (4%)
Frame = +3
Query: 261 LGRFAVRDMRQTVAVG-VIKAVN 326
+GRF +RD TVAVG V+K ++
Sbjct: 640 MGRFTLRDQGTTVAVGKVVKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 27.1 bits (57), Expect = 2.2
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +3
Query: 255 PPLGRFAVRDMRQTVAVGVIK 317
P LGRF +R TVA G++K
Sbjct: 569 PALGRFILRRSGDTVAAGIVK 589
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 6.7
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = -3
Query: 203 MAASPDLMDFGLTSVDLPVRRSTFS 129
++ +PDL D L+SVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
>SPBC418.01c |his4|SPBC887.20c|imidazoleglycerol-phosphate
synthase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 541
Score = 25.0 bits (52), Expect = 8.9
Identities = 18/61 (29%), Positives = 26/61 (42%)
Frame = +1
Query: 103 IACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFHPSVVLLSV 282
IAC + D K + + + KS + + NL LC FQE VV L++
Sbjct: 233 IACLDVRSNDAGDLVVTKGDQYDVRE-KSSGSEVRNLGKPVELCQRYFQEGADEVVFLNI 291
Query: 283 T 285
T
Sbjct: 292 T 292
>SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 25.0 bits (52), Expect = 8.9
Identities = 10/37 (27%), Positives = 19/37 (51%)
Frame = -1
Query: 382 PLVAFSAALVTLPPPASLKLTALMTPTATVCLMSRTA 272
P + ++ T+PP S+ T + PT ++ + TA
Sbjct: 73 PSTSHNSTTTTVPPTTSMNTTTTVPPTTSLNTTTTTA 109
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,470,786
Number of Sequences: 5004
Number of extensions: 47572
Number of successful extensions: 146
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 138
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 146
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 275671126
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -